Fathead minnow nidovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Tornidovirineae; Tobaniviridae; Piscanivirinae; Bafinivirus; Pimfabavirus; Fathead minnow nidovirus 1

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6LMN2|I6LMN2_9NIDO Membrane protein OS=Fathead minnow nidovirus OX=889873 GN=M PE=4 SV=1
MM1 pKa = 7.31MLLILPLILLCSSTHH16 pKa = 6.47AYY18 pKa = 9.16RR19 pKa = 11.84VQFVAPVPLNWINCYY34 pKa = 7.76TTDD37 pKa = 3.64CVSWQACPTTVAALVCVRR55 pKa = 11.84FTAVAPSFYY64 pKa = 10.8LSANNTLIGYY74 pKa = 8.4NADD77 pKa = 3.46PNTFYY82 pKa = 11.51DD83 pKa = 5.49LITMVQQSTYY93 pKa = 10.88SFSQAQTDD101 pKa = 3.92TPPTKK106 pKa = 10.18QCNGPSTILSGQCLTPADD124 pKa = 3.95SCLDD128 pKa = 3.86PLCPTTSFFEE138 pKa = 4.33TGSLDD143 pKa = 4.07SLTLDD148 pKa = 3.84TQCVASYY155 pKa = 10.32KK156 pKa = 10.64GYY158 pKa = 9.81GSVYY162 pKa = 9.03GTPCEE167 pKa = 4.02PFAPQLSPYY176 pKa = 7.91FTKK179 pKa = 10.68NVPNTPGFTDD189 pKa = 3.36YY190 pKa = 11.21GVNRR194 pKa = 11.84QSPKK198 pKa = 9.9LGCYY202 pKa = 7.25YY203 pKa = 10.21EE204 pKa = 4.71KK205 pKa = 9.75WAKK208 pKa = 9.84YY209 pKa = 9.42RR210 pKa = 11.84PKK212 pKa = 10.34PYY214 pKa = 9.56TATIDD219 pKa = 3.68SLAGCDD225 pKa = 2.8IVFFFVNTLSDD236 pKa = 3.11RR237 pKa = 11.84SGNMGVFEE245 pKa = 4.99RR246 pKa = 11.84EE247 pKa = 3.64EE248 pKa = 4.63GDD250 pKa = 3.32WNTIAAIKK258 pKa = 9.64TKK260 pKa = 10.47FPTTQVFLTFGGWTSDD276 pKa = 3.44NKK278 pKa = 10.52IMSAAMSNSTILNDD292 pKa = 3.06INTLSSRR299 pKa = 11.84LGVSVDD305 pKa = 4.06FDD307 pKa = 3.78IEE309 pKa = 4.51FPGSSNGNAAVFPYY323 pKa = 10.67DD324 pKa = 3.42EE325 pKa = 4.64ALITSFMTDD334 pKa = 1.69ICNFQHH340 pKa = 6.82SAGRR344 pKa = 11.84QCAWGGLSVGSHH356 pKa = 5.3WHH358 pKa = 5.49PTLINKK364 pKa = 9.75LSTIAGVDD372 pKa = 3.23YY373 pKa = 10.58FPIFGYY379 pKa = 10.34DD380 pKa = 3.3LHH382 pKa = 7.79GSWAPIPRR390 pKa = 11.84QQSALVNYY398 pKa = 10.27SPDD401 pKa = 3.45PTVSGGGPLNTYY413 pKa = 9.96SLITSVDD420 pKa = 3.2NFLTIVPASKK430 pKa = 10.71LILGLPMYY438 pKa = 10.83ARR440 pKa = 11.84GYY442 pKa = 10.78LVDD445 pKa = 4.01SSNSVLGSFPLTGPMLGPGEE465 pKa = 4.36TGSDD469 pKa = 3.91LIVTTTTYY477 pKa = 11.47GEE479 pKa = 4.37VPATYY484 pKa = 10.71NPVTDD489 pKa = 4.79TIRR492 pKa = 11.84RR493 pKa = 11.84DD494 pKa = 3.2PDD496 pKa = 3.13QPGFYY501 pKa = 9.9IMEE504 pKa = 4.64RR505 pKa = 11.84PLPSAQKK512 pKa = 10.47GLIYY516 pKa = 10.45FMNTTTFAHH525 pKa = 6.18IHH527 pKa = 5.34TVLKK531 pKa = 10.78AKK533 pKa = 10.4GVFQYY538 pKa = 11.41YY539 pKa = 10.36LYY541 pKa = 10.82ASSMDD546 pKa = 3.36QNVPSTSLKK555 pKa = 8.56YY556 pKa = 10.93SKK558 pKa = 10.17IIKK561 pKa = 8.53TNRR564 pKa = 11.84QRR566 pKa = 11.84RR567 pKa = 11.84AVNPSTVFYY576 pKa = 10.33NYY578 pKa = 7.21PTSAVNVPTNCPNIVLWAEE597 pKa = 4.01GLVATTSIYY606 pKa = 10.31QGCQRR611 pKa = 11.84EE612 pKa = 4.16NAASSLCWYY621 pKa = 8.77RR622 pKa = 11.84QSSFLCVPSTEE633 pKa = 4.05VTLITKK639 pKa = 6.91TTKK642 pKa = 9.57SCKK645 pKa = 10.26GINTNTLLPTSSASSVMCLDD665 pKa = 3.43LTLYY669 pKa = 6.7TTEE672 pKa = 4.53VSLCGNINAPTPTTKK687 pKa = 10.33VQLSLPSPDD696 pKa = 3.35SLTWGYY702 pKa = 11.34ASTPIYY708 pKa = 10.31SGYY711 pKa = 8.52LTHH714 pKa = 7.03TEE716 pKa = 4.49VISKK720 pKa = 10.21PPFCTSFTSLPSCAEE735 pKa = 4.54LICGSNSLCYY745 pKa = 9.77TKK747 pKa = 10.1LTEE750 pKa = 4.24FGKK753 pKa = 11.12LNMCTALGDD762 pKa = 4.46AISVYY767 pKa = 10.9NSVITDD773 pKa = 3.4IVEE776 pKa = 4.22SQATLDD782 pKa = 3.6ALYY785 pKa = 10.57TEE787 pKa = 4.64MLDD790 pKa = 3.64VAMTKK795 pKa = 10.54FGTVAQTRR803 pKa = 11.84KK804 pKa = 9.78KK805 pKa = 10.57RR806 pKa = 11.84FVEE809 pKa = 6.08FIALGVASVALVEE822 pKa = 4.14ATVAIGLAVANANDD836 pKa = 3.24IRR838 pKa = 11.84EE839 pKa = 4.09LRR841 pKa = 11.84GDD843 pKa = 3.73VKK845 pKa = 10.94NLRR848 pKa = 11.84DD849 pKa = 3.32EE850 pKa = 4.19SAANFKK856 pKa = 10.81AIEE859 pKa = 4.22EE860 pKa = 4.13KK861 pKa = 10.88HH862 pKa = 5.48NAFVKK867 pKa = 8.04TTADD871 pKa = 3.08GFAAQNEE878 pKa = 4.55VNKK881 pKa = 10.44GFNSNIDD888 pKa = 3.56TLQNSLKK895 pKa = 10.96DD896 pKa = 3.54MAIVTSQKK904 pKa = 10.33FKK906 pKa = 11.04EE907 pKa = 4.1LDD909 pKa = 3.21QAIQSLVSSINSLQLKK925 pKa = 10.22LDD927 pKa = 3.67TEE929 pKa = 4.58IQLSANSDD937 pKa = 3.51SALARR942 pKa = 11.84VLTALSLGHH951 pKa = 5.73RR952 pKa = 11.84TNRR955 pKa = 11.84NLDD958 pKa = 3.31RR959 pKa = 11.84HH960 pKa = 5.76ISQVRR965 pKa = 11.84TCLASIQQNSLAGCVNQGSNSIPLGIRR992 pKa = 11.84VFNEE996 pKa = 3.64TGDD999 pKa = 3.72LKK1001 pKa = 10.85IVFFYY1006 pKa = 9.87ATRR1009 pKa = 11.84QRR1011 pKa = 11.84LGYY1014 pKa = 9.6LQFKK1018 pKa = 8.64ATSAFCTNGTLVTAAPGCIFTANGTQIEE1046 pKa = 4.24HH1047 pKa = 6.35SKK1049 pKa = 10.34IGPLTPCGSSFVSLPIATCPTGSVDD1074 pKa = 3.55IEE1076 pKa = 4.4GVKK1079 pKa = 10.5PSLTPNFEE1087 pKa = 4.15VPQLEE1092 pKa = 4.46LKK1094 pKa = 10.19PPKK1097 pKa = 10.61LEE1099 pKa = 4.19VNLTNVNLSDD1109 pKa = 4.5YY1110 pKa = 10.03IRR1112 pKa = 11.84PITPVDD1118 pKa = 3.6LSKK1121 pKa = 10.85IEE1123 pKa = 4.31DD1124 pKa = 3.42LQARR1128 pKa = 11.84FDD1130 pKa = 4.06KK1131 pKa = 11.34LNLTLTEE1138 pKa = 4.43LSHH1141 pKa = 6.91ISSSGSGLPTWAIIFIVCVAVVIVLVFIVTIYY1173 pKa = 10.47MNTRR1177 pKa = 11.84QSTRR1181 pKa = 11.84LDD1183 pKa = 3.38SFLSKK1188 pKa = 10.31IRR1190 pKa = 3.6

Molecular weight: 129.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6LMN3|I6LMN3_9NIDO Nucleocapsid protein OS=Fathead minnow nidovirus OX=889873 GN=N PE=4 SV=1
MM1 pKa = 7.56SFPMQPAYY9 pKa = 10.47FPLQQRR15 pKa = 11.84QRR17 pKa = 11.84RR18 pKa = 11.84PRR20 pKa = 11.84AVAGAKK26 pKa = 9.84PKK28 pKa = 10.42FKK30 pKa = 10.52SAPPSPAVINAAVKK44 pKa = 10.64KK45 pKa = 9.73EE46 pKa = 4.06VSTHH50 pKa = 5.57IPMPPISMQEE60 pKa = 3.87KK61 pKa = 9.61SGLRR65 pKa = 11.84ALDD68 pKa = 3.19VRR70 pKa = 11.84NNFSTAEE77 pKa = 3.98LAGLYY82 pKa = 10.01AQLIQHH88 pKa = 7.18LNHH91 pKa = 6.08CHH93 pKa = 4.82GRR95 pKa = 11.84MVFMPGDD102 pKa = 4.08TISQGFVQVQLKK114 pKa = 10.33LRR116 pKa = 11.84LPSDD120 pKa = 3.29QCLKK124 pKa = 10.04MVSAHH129 pKa = 6.17SFAEE133 pKa = 3.87QRR135 pKa = 11.84LLASEE140 pKa = 5.04GEE142 pKa = 4.22ASVTTTLLSSLDD154 pKa = 3.66KK155 pKa = 11.05QAVNSLNAKK164 pKa = 9.89DD165 pKa = 3.42

Molecular weight: 18.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

13614

165

7174

2722.8

304.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.847 ± 0.515

1.814 ± 0.192

4.348 ± 0.19

4.415 ± 0.41

4.606 ± 0.102

4.26 ± 0.333

3.012 ± 0.371

6.339 ± 0.22

5.406 ± 0.361

9.079 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.035 ± 0.085

5.362 ± 0.124

5.428 ± 0.191

4.356 ± 0.169

2.637 ± 0.187

8.219 ± 0.336

10.842 ± 0.594

6.846 ± 0.172

0.654 ± 0.062

4.495 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski