Amino acid dipepetide frequency for Fathead minnow nidovirus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.554AlaAla: 4.554 ± 0.65
0.514AlaCys: 0.514 ± 0.244
2.13AlaAsp: 2.13 ± 0.348
1.983AlaGlu: 1.983 ± 0.862
2.938AlaPhe: 2.938 ± 0.536
3.526AlaGly: 3.526 ± 0.662
2.424AlaHis: 2.424 ± 0.416
3.453AlaIle: 3.453 ± 0.562
1.91AlaLys: 1.91 ± 1.089
5.509AlaLeu: 5.509 ± 0.381
0.882AlaMet: 0.882 ± 0.172
3.306AlaAsn: 3.306 ± 0.398
2.277AlaPro: 2.277 ± 0.757
2.718AlaGln: 2.718 ± 0.448
1.396AlaArg: 1.396 ± 0.262
5.069AlaSer: 5.069 ± 0.979
6.464AlaThr: 6.464 ± 0.519
4.04AlaVal: 4.04 ± 2.433
0.441AlaTrp: 0.441 ± 0.266
2.938AlaTyr: 2.938 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
1.322CysAla: 1.322 ± 0.248
0.514CysCys: 0.514 ± 0.133
0.661CysAsp: 0.661 ± 0.252
0.661CysGlu: 0.661 ± 0.1
0.661CysPhe: 0.661 ± 0.173
0.882CysGly: 0.882 ± 0.299
0.22CysHis: 0.22 ± 0.544
0.955CysIle: 0.955 ± 0.148
1.175CysLys: 1.175 ± 0.198
2.424CysLeu: 2.424 ± 0.561
0.22CysMet: 0.22 ± 0.251
1.102CysAsn: 1.102 ± 0.292
1.249CysPro: 1.249 ± 0.446
0.808CysGln: 0.808 ± 0.152
0.367CysArg: 0.367 ± 0.095
1.616CysSer: 1.616 ± 0.346
1.543CysThr: 1.543 ± 0.259
1.322CysVal: 1.322 ± 0.722
0.073CysTrp: 0.073 ± 0.136
0.367CysTyr: 0.367 ± 0.387
0.0CysXaa: 0.0 ± 0.0
Asp
1.249AspAla: 1.249 ± 0.207
1.102AspCys: 1.102 ± 0.195
1.69AspAsp: 1.69 ± 0.46
1.616AspGlu: 1.616 ± 0.239
2.277AspPhe: 2.277 ± 0.488
2.277AspGly: 2.277 ± 0.318
1.028AspHis: 1.028 ± 0.276
2.938AspIle: 2.938 ± 0.565
2.351AspLys: 2.351 ± 0.376
5.436AspLeu: 5.436 ± 0.831
1.249AspMet: 1.249 ± 0.547
2.645AspAsn: 2.645 ± 0.473
1.836AspPro: 1.836 ± 0.429
1.249AspGln: 1.249 ± 0.533
1.469AspArg: 1.469 ± 0.254
4.04AspSer: 4.04 ± 0.451
3.6AspThr: 3.6 ± 0.184
2.571AspVal: 2.571 ± 0.283
0.294AspTrp: 0.294 ± 0.101
2.498AspTyr: 2.498 ± 0.528
0.0AspXaa: 0.0 ± 0.0
Glu
2.13GluAla: 2.13 ± 0.484
0.367GluCys: 0.367 ± 0.095
2.791GluAsp: 2.791 ± 0.628
3.526GluGlu: 3.526 ± 0.753
1.91GluPhe: 1.91 ± 0.192
1.469GluGly: 1.469 ± 0.432
1.175GluHis: 1.175 ± 0.216
3.379GluIle: 3.379 ± 0.752
1.91GluLys: 1.91 ± 0.313
3.893GluLeu: 3.893 ± 0.2
1.175GluMet: 1.175 ± 0.216
1.91GluAsn: 1.91 ± 0.377
2.13GluPro: 2.13 ± 0.435
1.983GluGln: 1.983 ± 0.472
1.396GluArg: 1.396 ± 0.231
3.673GluSer: 3.673 ± 0.755
4.114GluThr: 4.114 ± 0.734
3.379GluVal: 3.379 ± 0.635
0.294GluTrp: 0.294 ± 0.084
1.543GluTyr: 1.543 ± 0.421
0.0GluXaa: 0.0 ± 0.0
Phe
1.836PheAla: 1.836 ± 0.355
0.735PheCys: 0.735 ± 0.221
1.543PheAsp: 1.543 ± 0.238
2.351PheGlu: 2.351 ± 0.345
3.232PhePhe: 3.232 ± 0.478
1.69PheGly: 1.69 ± 0.407
1.396PheHis: 1.396 ± 0.397
3.085PheIle: 3.085 ± 0.292
2.057PheLys: 2.057 ± 0.318
2.938PheLeu: 2.938 ± 0.223
1.028PheMet: 1.028 ± 0.482
1.983PheAsn: 1.983 ± 0.14
2.791PhePro: 2.791 ± 0.821
2.057PheGln: 2.057 ± 0.136
1.028PheArg: 1.028 ± 0.266
4.408PheSer: 4.408 ± 0.818
4.848PheThr: 4.848 ± 0.467
4.187PheVal: 4.187 ± 0.267
0.147PheTrp: 0.147 ± 0.042
2.718PheTyr: 2.718 ± 0.325
0.0PheXaa: 0.0 ± 0.0
Gly
1.836GlyAla: 1.836 ± 0.789
1.469GlyCys: 1.469 ± 0.534
3.012GlyAsp: 3.012 ± 0.262
1.69GlyGlu: 1.69 ± 0.349
1.763GlyPhe: 1.763 ± 0.922
1.616GlyGly: 1.616 ± 0.375
1.69GlyHis: 1.69 ± 0.173
2.351GlyIle: 2.351 ± 0.401
2.057GlyLys: 2.057 ± 0.28
3.6GlyLeu: 3.6 ± 0.929
0.808GlyMet: 0.808 ± 0.215
2.13GlyAsn: 2.13 ± 0.254
1.983GlyPro: 1.983 ± 0.451
0.735GlyGln: 0.735 ± 0.112
1.028GlyArg: 1.028 ± 0.425
4.775GlySer: 4.775 ± 1.061
3.673GlyThr: 3.673 ± 0.334
3.453GlyVal: 3.453 ± 0.521
0.147GlyTrp: 0.147 ± 0.128
2.791GlyTyr: 2.791 ± 0.62
0.0GlyXaa: 0.0 ± 0.0
His
2.498HisAla: 2.498 ± 0.338
0.735HisCys: 0.735 ± 0.476
0.955HisAsp: 0.955 ± 0.248
0.955HisGlu: 0.955 ± 0.248
1.028HisPhe: 1.028 ± 0.233
1.469HisGly: 1.469 ± 0.58
0.808HisHis: 0.808 ± 0.215
2.13HisIle: 2.13 ± 0.533
1.836HisLys: 1.836 ± 0.369
2.718HisLeu: 2.718 ± 0.572
0.882HisMet: 0.882 ± 0.227
1.836HisAsn: 1.836 ± 0.588
1.249HisPro: 1.249 ± 0.136
1.102HisGln: 1.102 ± 0.284
1.028HisArg: 1.028 ± 0.167
2.424HisSer: 2.424 ± 0.366
2.938HisThr: 2.938 ± 0.538
1.836HisVal: 1.836 ± 0.334
0.147HisTrp: 0.147 ± 0.128
1.543HisTyr: 1.543 ± 0.432
0.0HisXaa: 0.0 ± 0.0
Ile
2.351IleAla: 2.351 ± 0.36
1.322IleCys: 1.322 ± 0.166
2.865IleAsp: 2.865 ± 0.517
3.673IleGlu: 3.673 ± 0.561
3.673IlePhe: 3.673 ± 0.411
2.277IleGly: 2.277 ± 0.237
2.204IleHis: 2.204 ± 0.46
3.673IleIle: 3.673 ± 0.459
3.6IleLys: 3.6 ± 0.702
5.73IleLeu: 5.73 ± 0.508
1.102IleMet: 1.102 ± 0.218
3.526IleAsn: 3.526 ± 0.686
3.453IlePro: 3.453 ± 0.568
2.057IleGln: 2.057 ± 0.318
1.763IleArg: 1.763 ± 0.462
5.289IleSer: 5.289 ± 0.66
6.979IleThr: 6.979 ± 1.223
3.6IleVal: 3.6 ± 0.884
0.514IleTrp: 0.514 ± 0.16
3.746IleTyr: 3.746 ± 0.368
0.0IleXaa: 0.0 ± 0.0
Lys
1.543LysAla: 1.543 ± 0.29
0.808LysCys: 0.808 ± 0.261
1.396LysAsp: 1.396 ± 0.595
3.379LysGlu: 3.379 ± 0.548
3.232LysPhe: 3.232 ± 0.239
1.836LysGly: 1.836 ± 0.467
2.571LysHis: 2.571 ± 0.552
3.379LysIle: 3.379 ± 0.389
2.277LysLys: 2.277 ± 0.497
5.73LysLeu: 5.73 ± 0.307
0.735LysMet: 0.735 ± 0.476
2.791LysAsn: 2.791 ± 0.515
5.363LysPro: 5.363 ± 0.391
3.159LysGln: 3.159 ± 0.473
1.469LysArg: 1.469 ± 0.295
3.453LysSer: 3.453 ± 0.786
3.967LysThr: 3.967 ± 0.625
4.334LysVal: 4.334 ± 0.998
0.294LysTrp: 0.294 ± 0.101
2.351LysTyr: 2.351 ± 0.191
0.0LysXaa: 0.0 ± 0.0
Leu
5.656LeuAla: 5.656 ± 1.018
3.012LeuCys: 3.012 ± 0.487
4.481LeuAsp: 4.481 ± 1.128
3.085LeuGlu: 3.085 ± 0.588
3.306LeuPhe: 3.306 ± 0.649
4.775LeuGly: 4.775 ± 0.883
3.085LeuHis: 3.085 ± 0.675
4.848LeuIle: 4.848 ± 1.091
6.317LeuLys: 6.317 ± 0.457
7.052LeuLeu: 7.052 ± 2.143
1.763LeuMet: 1.763 ± 0.418
4.187LeuAsn: 4.187 ± 0.577
4.334LeuPro: 4.334 ± 0.49
4.261LeuGln: 4.261 ± 0.287
3.159LeuArg: 3.159 ± 0.739
5.656LeuSer: 5.656 ± 1.202
11.46LeuThr: 11.46 ± 1.137
6.171LeuVal: 6.171 ± 0.589
0.441LeuTrp: 0.441 ± 0.261
3.746LeuTyr: 3.746 ± 0.309
0.0LeuXaa: 0.0 ± 0.0
Met
0.808MetAla: 0.808 ± 0.163
0.441MetCys: 0.441 ± 0.227
1.249MetAsp: 1.249 ± 0.219
0.588MetGlu: 0.588 ± 0.091
0.588MetPhe: 0.588 ± 0.154
1.249MetGly: 1.249 ± 0.136
0.367MetHis: 0.367 ± 0.095
1.028MetIle: 1.028 ± 0.266
1.175MetLys: 1.175 ± 0.306
3.159MetLeu: 3.159 ± 0.277
0.294MetMet: 0.294 ± 0.101
1.102MetAsn: 1.102 ± 0.189
0.514MetPro: 0.514 ± 1.063
1.102MetGln: 1.102 ± 0.984
0.367MetArg: 0.367 ± 0.095
1.102MetSer: 1.102 ± 1.211
1.249MetThr: 1.249 ± 0.186
1.983MetVal: 1.983 ± 0.866
0.147MetTrp: 0.147 ± 0.042
1.836MetTyr: 1.836 ± 0.269
0.0MetXaa: 0.0 ± 0.0
Asn
2.938AsnAla: 2.938 ± 0.859
0.808AsnCys: 0.808 ± 0.295
1.616AsnAsp: 1.616 ± 0.267
2.791AsnGlu: 2.791 ± 0.285
2.645AsnPhe: 2.645 ± 0.472
2.791AsnGly: 2.791 ± 0.219
1.616AsnHis: 1.616 ± 0.513
4.261AsnIle: 4.261 ± 0.768
3.159AsnLys: 3.159 ± 0.509
5.363AsnLeu: 5.363 ± 0.372
1.69AsnMet: 1.69 ± 0.256
3.453AsnAsn: 3.453 ± 0.516
2.571AsnPro: 2.571 ± 0.472
2.057AsnGln: 2.057 ± 0.334
1.396AsnArg: 1.396 ± 0.284
3.746AsnSer: 3.746 ± 0.815
4.775AsnThr: 4.775 ± 0.885
2.498AsnVal: 2.498 ± 0.278
0.808AsnTrp: 0.808 ± 0.119
2.645AsnTyr: 2.645 ± 0.504
0.0AsnXaa: 0.0 ± 0.0
Pro
2.424ProAla: 2.424 ± 1.024
0.514ProCys: 0.514 ± 0.207
2.498ProAsp: 2.498 ± 0.408
1.836ProGlu: 1.836 ± 0.33
1.983ProPhe: 1.983 ± 0.386
1.763ProGly: 1.763 ± 0.583
1.616ProHis: 1.616 ± 0.291
3.6ProIle: 3.6 ± 0.315
3.012ProLys: 3.012 ± 0.361
4.775ProLeu: 4.775 ± 0.59
1.028ProMet: 1.028 ± 1.027
3.012ProAsn: 3.012 ± 0.489
3.085ProPro: 3.085 ± 0.924
2.277ProGln: 2.277 ± 0.377
0.955ProArg: 0.955 ± 0.463
5.289ProSer: 5.289 ± 0.643
8.301ProThr: 8.301 ± 1.053
3.306ProVal: 3.306 ± 0.554
0.514ProTrp: 0.514 ± 0.133
2.498ProTyr: 2.498 ± 0.377
0.0ProXaa: 0.0 ± 0.0
Gln
3.746GlnAla: 3.746 ± 0.176
1.322GlnCys: 1.322 ± 0.561
1.69GlnAsp: 1.69 ± 0.503
1.322GlnGlu: 1.322 ± 0.635
1.616GlnPhe: 1.616 ± 0.239
1.396GlnGly: 1.396 ± 0.615
1.249GlnHis: 1.249 ± 0.397
3.012GlnIle: 3.012 ± 0.469
2.13GlnLys: 2.13 ± 0.36
2.791GlnLeu: 2.791 ± 0.837
0.735GlnMet: 0.735 ± 0.189
2.351GlnAsn: 2.351 ± 0.36
1.396GlnPro: 1.396 ± 0.381
3.673GlnGln: 3.673 ± 0.365
1.028GlnArg: 1.028 ± 1.63
4.334GlnSer: 4.334 ± 0.633
5.142GlnThr: 5.142 ± 0.673
3.012GlnVal: 3.012 ± 0.349
0.367GlnTrp: 0.367 ± 0.095
1.249GlnTyr: 1.249 ± 0.222
0.0GlnXaa: 0.0 ± 0.0
Arg
1.396ArgAla: 1.396 ± 0.954
0.294ArgCys: 0.294 ± 0.101
1.102ArgAsp: 1.102 ± 0.189
1.469ArgGlu: 1.469 ± 0.281
1.175ArgPhe: 1.175 ± 0.277
1.322ArgGly: 1.322 ± 0.2
0.441ArgHis: 0.441 ± 0.225
1.763ArgIle: 1.763 ± 0.454
1.616ArgLys: 1.616 ± 0.306
2.498ArgLeu: 2.498 ± 0.796
1.396ArgMet: 1.396 ± 0.395
1.175ArgAsn: 1.175 ± 0.633
1.469ArgPro: 1.469 ± 0.432
1.763ArgGln: 1.763 ± 1.007
1.469ArgArg: 1.469 ± 0.389
1.396ArgSer: 1.396 ± 0.252
1.983ArgThr: 1.983 ± 0.336
1.836ArgVal: 1.836 ± 0.201
0.0ArgTrp: 0.0 ± 0.0
0.808ArgTyr: 0.808 ± 0.214
0.0ArgXaa: 0.0 ± 0.0
Ser
6.024SerAla: 6.024 ± 1.019
1.175SerCys: 1.175 ± 0.267
3.82SerAsp: 3.82 ± 0.265
3.893SerGlu: 3.893 ± 0.779
3.012SerPhe: 3.012 ± 1.339
4.261SerGly: 4.261 ± 0.33
1.69SerHis: 1.69 ± 0.152
3.893SerIle: 3.893 ± 0.444
5.069SerLys: 5.069 ± 0.767
8.154SerLeu: 8.154 ± 1.358
0.514SerMet: 0.514 ± 0.749
3.6SerAsn: 3.6 ± 0.545
3.82SerPro: 3.82 ± 0.236
3.526SerGln: 3.526 ± 0.236
0.955SerArg: 0.955 ± 0.246
7.052SerSer: 7.052 ± 0.679
10.284SerThr: 10.284 ± 0.771
7.419SerVal: 7.419 ± 0.41
0.808SerTrp: 0.808 ± 0.188
3.232SerTyr: 3.232 ± 0.526
0.0SerXaa: 0.0 ± 0.0
Thr
7.419ThrAla: 7.419 ± 0.29
1.469ThrCys: 1.469 ± 0.224
4.922ThrAsp: 4.922 ± 0.728
5.583ThrGlu: 5.583 ± 0.843
5.216ThrPhe: 5.216 ± 0.774
3.012ThrGly: 3.012 ± 0.455
3.6ThrHis: 3.6 ± 0.804
6.979ThrIle: 6.979 ± 0.256
4.995ThrLys: 4.995 ± 0.668
9.256ThrLeu: 9.256 ± 0.398
2.645ThrMet: 2.645 ± 0.585
5.069ThrAsn: 5.069 ± 0.51
9.182ThrPro: 9.182 ± 1.533
4.187ThrGln: 4.187 ± 0.754
1.69ThrArg: 1.69 ± 0.404
9.403ThrSer: 9.403 ± 1.214
13.002ThrThr: 13.002 ± 1.121
6.685ThrVal: 6.685 ± 0.871
0.735ThrTrp: 0.735 ± 0.198
3.379ThrTyr: 3.379 ± 0.276
0.0ThrXaa: 0.0 ± 0.0
Val
4.775ValAla: 4.775 ± 1.534
0.661ValCys: 0.661 ± 0.206
3.012ValAsp: 3.012 ± 0.52
2.645ValGlu: 2.645 ± 0.429
3.232ValPhe: 3.232 ± 0.907
2.424ValGly: 2.424 ± 0.514
1.616ValHis: 1.616 ± 0.43
3.6ValIle: 3.6 ± 0.333
4.334ValLys: 4.334 ± 0.362
6.317ValLeu: 6.317 ± 0.553
1.322ValMet: 1.322 ± 0.197
4.554ValAsn: 4.554 ± 0.978
3.526ValPro: 3.526 ± 0.67
3.159ValGln: 3.159 ± 0.9
2.938ValArg: 2.938 ± 0.321
5.069ValSer: 5.069 ± 0.65
8.815ValThr: 8.815 ± 0.968
3.82ValVal: 3.82 ± 0.565
0.367ValTrp: 0.367 ± 0.095
2.277ValTyr: 2.277 ± 0.377
0.0ValXaa: 0.0 ± 0.0
Trp
0.661TrpAla: 0.661 ± 0.585
0.073TrpCys: 0.073 ± 0.046
0.294TrpAsp: 0.294 ± 0.186
0.294TrpGlu: 0.294 ± 0.084
0.294TrpPhe: 0.294 ± 0.236
0.147TrpGly: 0.147 ± 0.272
0.22TrpHis: 0.22 ± 0.111
0.661TrpIle: 0.661 ± 0.123
0.294TrpLys: 0.294 ± 0.186
0.808TrpLeu: 0.808 ± 0.214
0.0TrpMet: 0.0 ± 0.0
0.661TrpAsn: 0.661 ± 0.1
0.073TrpPro: 0.073 ± 0.046
0.22TrpGln: 0.22 ± 0.111
0.147TrpArg: 0.147 ± 0.042
0.22TrpSer: 0.22 ± 0.062
0.367TrpThr: 0.367 ± 0.11
0.588TrpVal: 0.588 ± 0.168
0.0TrpTrp: 0.0 ± 0.0
0.514TrpTyr: 0.514 ± 0.104
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.306TyrAla: 3.306 ± 0.323
0.808TyrCys: 0.808 ± 0.215
1.763TyrAsp: 1.763 ± 0.374
0.808TyrGlu: 0.808 ± 0.119
2.277TyrPhe: 2.277 ± 0.484
2.424TyrGly: 2.424 ± 0.364
1.102TyrHis: 1.102 ± 0.412
4.408TyrIle: 4.408 ± 0.905
2.791TyrLys: 2.791 ± 0.628
2.645TyrLeu: 2.645 ± 0.331
0.808TyrMet: 0.808 ± 0.163
3.232TyrAsn: 3.232 ± 0.496
1.983TyrPro: 1.983 ± 0.245
1.469TyrGln: 1.469 ± 0.324
1.322TyrArg: 1.322 ± 0.29
4.187TyrSer: 4.187 ± 0.623
4.922TyrThr: 4.922 ± 0.772
2.424TyrVal: 2.424 ± 0.472
0.0TyrTrp: 0.0 ± 0.0
2.277TyrTyr: 2.277 ± 0.55
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5 proteins (13614 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski