Wallal virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3RCS2|U3RCS2_9REOV Outer capsid protein VP2 OS=Wallal virus OX=40061 PE=3 SV=1
MM1 pKa = 7.17EE2 pKa = 4.63QRR4 pKa = 11.84TQLRR8 pKa = 11.84RR9 pKa = 11.84VFPKK13 pKa = 9.44TVCVLDD19 pKa = 3.98PKK21 pKa = 11.37GEE23 pKa = 4.49TICGMIAKK31 pKa = 9.04SQHH34 pKa = 5.8KK35 pKa = 9.11PYY37 pKa = 10.63CLVRR41 pKa = 11.84TGRR44 pKa = 11.84VICLKK49 pKa = 10.45AISQPPPKK57 pKa = 10.25AYY59 pKa = 10.07VLEE62 pKa = 4.32VVKK65 pKa = 10.86CGAYY69 pKa = 10.14RR70 pKa = 11.84LIDD73 pKa = 4.01GQDD76 pKa = 3.32QISIMLNKK84 pKa = 10.22DD85 pKa = 3.53CVEE88 pKa = 4.14MTSEE92 pKa = 4.78RR93 pKa = 11.84WEE95 pKa = 3.78EE96 pKa = 3.64WQYY99 pKa = 11.89EE100 pKa = 4.32MVSIKK105 pKa = 10.64PMVVSLQLEE114 pKa = 4.47YY115 pKa = 11.16GVVDD119 pKa = 4.52AEE121 pKa = 4.24LKK123 pKa = 9.0YY124 pKa = 10.91GKK126 pKa = 10.43GFGSVEE132 pKa = 3.96PYY134 pKa = 10.32KK135 pKa = 10.94KK136 pKa = 10.35NEE138 pKa = 4.01LEE140 pKa = 4.12RR141 pKa = 11.84GEE143 pKa = 4.86VPTLPGVQQSAVGLRR158 pKa = 11.84EE159 pKa = 4.19LRR161 pKa = 11.84TKK163 pKa = 10.43LKK165 pKa = 10.5KK166 pKa = 10.0EE167 pKa = 3.96RR168 pKa = 11.84EE169 pKa = 4.12EE170 pKa = 4.09KK171 pKa = 9.34VQGRR175 pKa = 11.84SVEE178 pKa = 4.12LPTMKK183 pKa = 10.22KK184 pKa = 10.56VNVQAEE190 pKa = 4.21QGFEE194 pKa = 3.52WSAVRR199 pKa = 11.84EE200 pKa = 4.23KK201 pKa = 10.62VVEE204 pKa = 4.03MEE206 pKa = 4.74EE207 pKa = 4.49EE208 pKa = 3.88IKK210 pKa = 10.76DD211 pKa = 3.65WAEE214 pKa = 4.05HH215 pKa = 5.94GSSEE219 pKa = 5.14EE220 pKa = 4.17EE221 pKa = 4.27INDD224 pKa = 3.58KK225 pKa = 10.62QQTDD229 pKa = 3.63DD230 pKa = 4.01EE231 pKa = 4.83EE232 pKa = 4.64VVKK235 pKa = 11.03DD236 pKa = 4.34DD237 pKa = 5.03DD238 pKa = 5.61DD239 pKa = 3.96EE240 pKa = 4.42ATEE243 pKa = 4.11EE244 pKa = 4.36GLVKK248 pKa = 10.26VDD250 pKa = 3.92SFITGDD256 pKa = 3.51YY257 pKa = 9.61VNYY260 pKa = 9.92VSGMSKK266 pKa = 10.48KK267 pKa = 9.99KK268 pKa = 10.28DD269 pKa = 3.41EE270 pKa = 4.42RR271 pKa = 11.84LSGLSLSFPKK281 pKa = 10.17IEE283 pKa = 4.27GEE285 pKa = 4.13FEE287 pKa = 3.82KK288 pKa = 11.0VLCIKK293 pKa = 9.73KK294 pKa = 8.42TEE296 pKa = 4.06QTGFPVFEE304 pKa = 4.19VDD306 pKa = 3.67QATKK310 pKa = 9.09TFRR313 pKa = 11.84FRR315 pKa = 11.84MIGKK319 pKa = 8.62CDD321 pKa = 2.96RR322 pKa = 11.84VFVVKK327 pKa = 10.33RR328 pKa = 11.84SMSMVYY334 pKa = 10.21LPAGGPLL341 pKa = 3.3

Molecular weight: 38.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3RHX5|U3RHX5_9REOV RNA-directed RNA polymerase OS=Wallal virus OX=40061 PE=3 SV=1
MM1 pKa = 7.56LSEE4 pKa = 3.89LAARR8 pKa = 11.84FEE10 pKa = 4.53SEE12 pKa = 3.97KK13 pKa = 10.4VRR15 pKa = 11.84YY16 pKa = 9.15SATEE20 pKa = 3.44QQLITKK26 pKa = 9.37EE27 pKa = 4.09EE28 pKa = 4.03EE29 pKa = 3.71EE30 pKa = 4.66PIYY33 pKa = 11.15EE34 pKa = 4.26RR35 pKa = 11.84MDD37 pKa = 3.63KK38 pKa = 11.05AVVPYY43 pKa = 10.1SRR45 pKa = 11.84PPSYY49 pKa = 11.25VPTAPSGVSGKK60 pKa = 10.15DD61 pKa = 3.18VSLDD65 pKa = 3.23ILNNAMSNTTGATNAMKK82 pKa = 9.8MEE84 pKa = 3.92KK85 pKa = 8.9TAYY88 pKa = 9.82SSYY91 pKa = 11.01AEE93 pKa = 4.15AMRR96 pKa = 11.84DD97 pKa = 3.57SVPVQNVKK105 pKa = 9.99TSVNALIIPRR115 pKa = 11.84LRR117 pKa = 11.84NEE119 pKa = 3.7LSGLKK124 pKa = 9.8RR125 pKa = 11.84KK126 pKa = 8.23RR127 pKa = 11.84TLVHH131 pKa = 6.06IALIVTAAVTMITSFSSLVKK151 pKa = 10.27DD152 pKa = 3.99FQVSIPSGTEE162 pKa = 3.62SGNSTVTVQVPKK174 pKa = 10.13WFASFSAVFNTVNLVATGLMISFARR199 pKa = 11.84MEE201 pKa = 4.3KK202 pKa = 9.87MLDD205 pKa = 3.48GQIGMIKK212 pKa = 9.98KK213 pKa = 10.15EE214 pKa = 3.9IMKK217 pKa = 10.35KK218 pKa = 9.4EE219 pKa = 4.22SYY221 pKa = 10.73NEE223 pKa = 3.85AVRR226 pKa = 11.84KK227 pKa = 9.57SVTAIDD233 pKa = 3.75MTSLFQEE240 pKa = 4.32AEE242 pKa = 4.21GANGG246 pKa = 3.23

Molecular weight: 26.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6186

246

1306

618.6

71.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.24 ± 0.527

1.099 ± 0.285

5.706 ± 0.285

7.646 ± 0.528

3.831 ± 0.235

5.48 ± 0.381

2.069 ± 0.227

6.579 ± 0.435

6.531 ± 0.787

8.697 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.767 ± 0.309

4.64 ± 0.255

3.831 ± 0.332

3.686 ± 0.31

6.466 ± 0.336

5.674 ± 0.369

5.318 ± 0.373

7.355 ± 0.363

1.406 ± 0.265

3.977 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski