Clostridium amylolyticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3949 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6KKU0|A0A1M6KKU0_9CLOT Cell division protein FtsQ OS=Clostridium amylolyticum OX=1121298 GN=SAMN05444401_3400 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 5.26RR3 pKa = 11.84EE4 pKa = 4.94GYY6 pKa = 9.41VSLWIGNIKK15 pKa = 9.49TDD17 pKa = 4.33DD18 pKa = 3.68EE19 pKa = 4.23LLEE22 pKa = 4.19YY23 pKa = 11.02VEE25 pKa = 5.91LVYY28 pKa = 10.85TDD30 pKa = 3.53AGDD33 pKa = 3.57WLPSQFLKK41 pKa = 10.96DD42 pKa = 3.77FNIDD46 pKa = 3.13IDD48 pKa = 5.74DD49 pKa = 4.7FDD51 pKa = 6.16DD52 pKa = 4.18DD53 pKa = 4.51CIEE56 pKa = 4.32RR57 pKa = 11.84VCLEE61 pKa = 4.01QNVTSITEE69 pKa = 4.74LISGCSYY76 pKa = 11.05EE77 pKa = 5.53DD78 pKa = 3.2IVIPRR83 pKa = 11.84YY84 pKa = 9.81EE85 pKa = 4.72KK86 pKa = 9.57ITDD89 pKa = 3.44GVSIVKK95 pKa = 10.14CNSGILLYY103 pKa = 10.8NFQYY107 pKa = 10.92DD108 pKa = 4.08GNVKK112 pKa = 10.48SINNKK117 pKa = 9.72DD118 pKa = 3.5YY119 pKa = 9.99EE120 pKa = 4.51FKK122 pKa = 10.72FISSVEE128 pKa = 3.93YY129 pKa = 10.82GKK131 pKa = 10.84

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6FUZ0|A0A1M6FUZ0_9CLOT tRNA modification GTPase MnmE OS=Clostridium amylolyticum OX=1121298 GN=mnmE PE=3 SV=1
MM1 pKa = 7.28SEE3 pKa = 3.6KK4 pKa = 10.53RR5 pKa = 11.84EE6 pKa = 3.94PRR8 pKa = 11.84RR9 pKa = 11.84GNSRR13 pKa = 11.84MRR15 pKa = 11.84RR16 pKa = 11.84AKK18 pKa = 10.29RR19 pKa = 11.84KK20 pKa = 8.8VCAFCVDD27 pKa = 3.39KK28 pKa = 11.15ADD30 pKa = 4.34SIDD33 pKa = 3.54YY34 pKa = 10.81KK35 pKa = 11.25DD36 pKa = 3.1IGKK39 pKa = 9.09LRR41 pKa = 11.84KK42 pKa = 9.36YY43 pKa = 8.13VTEE46 pKa = 4.22RR47 pKa = 11.84GKK49 pKa = 10.15ILPRR53 pKa = 11.84RR54 pKa = 11.84ISGTCAKK61 pKa = 9.97HH62 pKa = 5.27QRR64 pKa = 11.84QLTEE68 pKa = 3.85SVKK71 pKa = 10.01RR72 pKa = 11.84ARR74 pKa = 11.84NIALLPFTTEE84 pKa = 3.59

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3949

0

3949

1223844

29

2874

309.9

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.878 ± 0.038

1.055 ± 0.014

5.281 ± 0.027

7.413 ± 0.039

4.478 ± 0.03

6.406 ± 0.038

1.445 ± 0.013

9.708 ± 0.045

8.916 ± 0.039

9.307 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.017

6.078 ± 0.034

2.969 ± 0.02

2.391 ± 0.015

3.335 ± 0.024

6.375 ± 0.03

4.81 ± 0.027

6.415 ± 0.029

0.763 ± 0.013

4.238 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski