Amino acid dipepetide frequency for Clostridium amylolyticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.575AlaAla: 4.575 ± 0.077	0.669AlaCys: 0.669 ± 0.026	2.523AlaAsp: 2.523 ± 0.051	3.817AlaGlu: 3.817 ± 0.071	2.81AlaPhe: 2.81 ± 0.049	3.92AlaGly: 3.92 ± 0.054	0.872AlaHis: 0.872 ± 0.03	5.604AlaIle: 5.604 ± 0.071	4.628AlaLys: 4.628 ± 0.07	6.761AlaLeu: 6.761 ± 0.089	1.783AlaMet: 1.783 ± 0.036	2.468AlaAsn: 2.468 ± 0.048	1.486AlaPro: 1.486 ± 0.041	1.494AlaGln: 1.494 ± 0.035	1.981AlaArg: 1.981 ± 0.044	3.618AlaSer: 3.618 ± 0.054	2.583AlaThr: 2.583 ± 0.057	4.459AlaVal: 4.459 ± 0.06	0.418AlaTrp: 0.418 ± 0.018	2.313AlaTyr: 2.313 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.592CysAla: 0.592 ± 0.023	0.158CysCys: 0.158 ± 0.015	0.582CysAsp: 0.582 ± 0.023	0.674CysGlu: 0.674 ± 0.019	0.461CysPhe: 0.461 ± 0.018	0.954CysGly: 0.954 ± 0.032	0.222CysHis: 0.222 ± 0.013	1.016CysIle: 1.016 ± 0.03	0.86CysLys: 0.86 ± 0.028	0.787CysLeu: 0.787 ± 0.028	0.253CysMet: 0.253 ± 0.015	0.601CysAsn: 0.601 ± 0.025	0.436CysPro: 0.436 ± 0.025	0.246CysGln: 0.246 ± 0.014	0.36CysArg: 0.36 ± 0.015	0.723CysSer: 0.723 ± 0.025	0.554CysThr: 0.554 ± 0.023	0.572CysVal: 0.572 ± 0.027	0.074CysTrp: 0.074 ± 0.008	0.423CysTyr: 0.423 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.683AspAla: 2.683 ± 0.051	0.486AspCys: 0.486 ± 0.02	2.298AspAsp: 2.298 ± 0.049	4.144AspGlu: 4.144 ± 0.065	2.831AspPhe: 2.831 ± 0.047	3.067AspGly: 3.067 ± 0.058	0.619AspHis: 0.619 ± 0.025	6.152AspIle: 6.152 ± 0.076	5.439AspLys: 5.439 ± 0.073	4.618AspLeu: 4.618 ± 0.07	1.596AspMet: 1.596 ± 0.038	3.294AspAsn: 3.294 ± 0.052	1.36AspPro: 1.36 ± 0.037	0.686AspGln: 0.686 ± 0.025	1.634AspArg: 1.634 ± 0.034	3.048AspSer: 3.048 ± 0.056	2.509AspThr: 2.509 ± 0.045	3.224AspVal: 3.224 ± 0.051	0.386AspTrp: 0.386 ± 0.019	2.732AspTyr: 2.732 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	4.514GluAla: 4.514 ± 0.073	0.581GluCys: 0.581 ± 0.023	4.528GluAsp: 4.528 ± 0.07	7.191GluGlu: 7.191 ± 0.09	3.008GluPhe: 3.008 ± 0.056	4.351GluGly: 4.351 ± 0.067	0.909GluHis: 0.909 ± 0.027	6.962GluIle: 6.962 ± 0.079	7.449GluLys: 7.449 ± 0.089	6.723GluLeu: 6.723 ± 0.081	1.968GluMet: 1.968 ± 0.043	4.988GluAsn: 4.988 ± 0.058	1.517GluPro: 1.517 ± 0.032	1.727GluGln: 1.727 ± 0.043	2.618GluArg: 2.618 ± 0.046	3.635GluSer: 3.635 ± 0.052	2.857GluThr: 2.857 ± 0.044	5.07GluVal: 5.07 ± 0.073	0.463GluTrp: 0.463 ± 0.02	3.071GluTyr: 3.071 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.38PheAla: 2.38 ± 0.047	0.486PheCys: 0.486 ± 0.02	2.231PheAsp: 2.231 ± 0.043	2.504PheGlu: 2.504 ± 0.046	2.075PhePhe: 2.075 ± 0.049	2.773PheGly: 2.773 ± 0.047	0.675PheHis: 0.675 ± 0.023	4.858PheIle: 4.858 ± 0.081	3.932PheLys: 3.932 ± 0.054	4.053PheLeu: 4.053 ± 0.065	1.306PheMet: 1.306 ± 0.032	3.065PheAsn: 3.065 ± 0.048	1.345PhePro: 1.345 ± 0.034	1.234PheGln: 1.234 ± 0.031	1.27PheArg: 1.27 ± 0.029	3.252PheSer: 3.252 ± 0.058	2.477PheThr: 2.477 ± 0.041	2.575PheVal: 2.575 ± 0.049	0.376PheTrp: 0.376 ± 0.019	1.916PheTyr: 1.916 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	4.186GlyAla: 4.186 ± 0.071	0.791GlyCys: 0.791 ± 0.028	3.143GlyAsp: 3.143 ± 0.064	4.372GlyGlu: 4.372 ± 0.063	3.223GlyPhe: 3.223 ± 0.064	4.266GlyGly: 4.266 ± 0.072	0.987GlyHis: 0.987 ± 0.031	6.727GlyIle: 6.727 ± 0.086	5.667GlyLys: 5.667 ± 0.078	5.411GlyLeu: 5.411 ± 0.082	1.781GlyMet: 1.781 ± 0.041	3.228GlyAsn: 3.228 ± 0.066	1.243GlyPro: 1.243 ± 0.033	1.456GlyGln: 1.456 ± 0.038	2.165GlyArg: 2.165 ± 0.043	3.724GlySer: 3.724 ± 0.062	3.363GlyThr: 3.363 ± 0.061	4.711GlyVal: 4.711 ± 0.067	0.556GlyTrp: 0.556 ± 0.023	3.06GlyTyr: 3.06 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	0.718HisAla: 0.718 ± 0.025	0.232HisCys: 0.232 ± 0.015	0.689HisAsp: 0.689 ± 0.021	0.944HisGlu: 0.944 ± 0.03	0.663HisPhe: 0.663 ± 0.027	1.078HisGly: 1.078 ± 0.031	0.353HisHis: 0.353 ± 0.021	1.379HisIle: 1.379 ± 0.037	1.169HisLys: 1.169 ± 0.031	1.256HisLeu: 1.256 ± 0.032	0.391HisMet: 0.391 ± 0.018	0.882HisAsn: 0.882 ± 0.03	0.645HisPro: 0.645 ± 0.025	0.313HisGln: 0.313 ± 0.015	0.605HisArg: 0.605 ± 0.021	0.898HisSer: 0.898 ± 0.028	0.646HisThr: 0.646 ± 0.023	0.784HisVal: 0.784 ± 0.023	0.157HisTrp: 0.157 ± 0.012	0.643HisTyr: 0.643 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.794IleAla: 5.794 ± 0.079	1.039IleCys: 1.039 ± 0.037	5.344IleAsp: 5.344 ± 0.063	6.61IleGlu: 6.61 ± 0.085	4.281IlePhe: 4.281 ± 0.065	5.766IleGly: 5.766 ± 0.084	1.278IleHis: 1.278 ± 0.038	9.586IleIle: 9.586 ± 0.112	8.741IleLys: 8.741 ± 0.085	9.025IleLeu: 9.025 ± 0.109	2.577IleMet: 2.577 ± 0.044	6.375IleAsn: 6.375 ± 0.08	3.469IlePro: 3.469 ± 0.054	2.182IleGln: 2.182 ± 0.04	2.991IleArg: 2.991 ± 0.053	6.855IleSer: 6.855 ± 0.079	5.21IleThr: 5.21 ± 0.072	5.72IleVal: 5.72 ± 0.071	0.581IleTrp: 0.581 ± 0.023	3.661IleTyr: 3.661 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	5.444LysAla: 5.444 ± 0.071	0.737LysCys: 0.737 ± 0.029	6.138LysAsp: 6.138 ± 0.075	8.705LysGlu: 8.705 ± 0.087	3.028LysPhe: 3.028 ± 0.046	5.424LysGly: 5.424 ± 0.066	1.214LysHis: 1.214 ± 0.037	7.594LysIle: 7.594 ± 0.081	7.55LysLys: 7.55 ± 0.088	7.652LysLeu: 7.652 ± 0.081	2.298LysMet: 2.298 ± 0.048	6.0LysAsn: 6.0 ± 0.074	2.289LysPro: 2.289 ± 0.039	2.075LysGln: 2.075 ± 0.047	2.957LysArg: 2.957 ± 0.052	5.215LysSer: 5.215 ± 0.084	3.863LysThr: 3.863 ± 0.065	6.234LysVal: 6.234 ± 0.081	0.654LysTrp: 0.654 ± 0.024	4.095LysTyr: 4.095 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	5.36LeuAla: 5.36 ± 0.075	1.057LeuCys: 1.057 ± 0.03	4.838LeuAsp: 4.838 ± 0.071	6.372LeuGlu: 6.372 ± 0.081	3.956LeuPhe: 3.956 ± 0.062	6.286LeuGly: 6.286 ± 0.076	1.205LeuHis: 1.205 ± 0.034	8.423LeuIle: 8.423 ± 0.123	8.59LeuLys: 8.59 ± 0.086	8.437LeuLeu: 8.437 ± 0.115	2.641LeuMet: 2.641 ± 0.052	5.934LeuAsn: 5.934 ± 0.064	3.129LeuPro: 3.129 ± 0.054	2.526LeuGln: 2.526 ± 0.042	3.201LeuArg: 3.201 ± 0.051	6.877LeuSer: 6.877 ± 0.084	4.671LeuThr: 4.671 ± 0.069	5.37LeuVal: 5.37 ± 0.076	0.712LeuTrp: 0.712 ± 0.027	3.485LeuTyr: 3.485 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	1.838MetAla: 1.838 ± 0.042	0.247MetCys: 0.247 ± 0.015	1.746MetAsp: 1.746 ± 0.041	2.142MetGlu: 2.142 ± 0.044	1.03MetPhe: 1.03 ± 0.031	1.869MetGly: 1.869 ± 0.039	0.413MetHis: 0.413 ± 0.02	2.204MetIle: 2.204 ± 0.049	2.623MetLys: 2.623 ± 0.043	2.508MetLeu: 2.508 ± 0.045	0.723MetMet: 0.723 ± 0.03	1.622MetAsn: 1.622 ± 0.037	0.97MetPro: 0.97 ± 0.026	0.726MetGln: 0.726 ± 0.025	0.977MetArg: 0.977 ± 0.029	1.714MetSer: 1.714 ± 0.044	1.094MetThr: 1.094 ± 0.035	1.905MetVal: 1.905 ± 0.039	0.167MetTrp: 0.167 ± 0.012	0.873MetTyr: 0.873 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.013AsnAla: 3.013 ± 0.053	0.61AsnCys: 0.61 ± 0.025	2.689AsnAsp: 2.689 ± 0.054	4.094AsnGlu: 4.094 ± 0.064	2.616AsnPhe: 2.616 ± 0.048	3.319AsnGly: 3.319 ± 0.063	0.941AsnHis: 0.941 ± 0.03	6.854AsnIle: 6.854 ± 0.085	5.95AsnLys: 5.95 ± 0.084	5.633AsnLeu: 5.633 ± 0.067	1.646AsnMet: 1.646 ± 0.031	4.354AsnAsn: 4.354 ± 0.068	2.181AsnPro: 2.181 ± 0.052	1.252AsnGln: 1.252 ± 0.032	1.92AsnArg: 1.92 ± 0.04	4.119AsnSer: 4.119 ± 0.069	2.935AsnThr: 2.935 ± 0.046	3.365AsnVal: 3.365 ± 0.054	0.476AsnTrp: 0.476 ± 0.021	2.817AsnTyr: 2.817 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	1.589ProAla: 1.589 ± 0.04	0.293ProCys: 0.293 ± 0.014	1.413ProAsp: 1.413 ± 0.034	2.593ProGlu: 2.593 ± 0.05	1.495ProPhe: 1.495 ± 0.039	1.894ProGly: 1.894 ± 0.045	0.543ProHis: 0.543 ± 0.02	2.723ProIle: 2.723 ± 0.045	2.281ProLys: 2.281 ± 0.046	2.857ProLeu: 2.857 ± 0.051	0.846ProMet: 0.846 ± 0.034	1.526ProAsn: 1.526 ± 0.036	0.704ProPro: 0.704 ± 0.024	0.9ProGln: 0.9 ± 0.025	0.853ProArg: 0.853 ± 0.03	1.907ProSer: 1.907 ± 0.042	1.476ProThr: 1.476 ± 0.033	2.13ProVal: 2.13 ± 0.052	0.289ProTrp: 0.289 ± 0.017	1.372ProTyr: 1.372 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	1.436GlnAla: 1.436 ± 0.039	0.308GlnCys: 0.308 ± 0.018	1.347GlnAsp: 1.347 ± 0.033	1.949GlnGlu: 1.949 ± 0.041	0.924GlnPhe: 0.924 ± 0.025	2.09GlnGly: 2.09 ± 0.045	0.335GlnHis: 0.335 ± 0.016	1.892GlnIle: 1.892 ± 0.043	1.966GlnLys: 1.966 ± 0.043	2.116GlnLeu: 2.116 ± 0.042	0.627GlnMet: 0.627 ± 0.022	1.321GlnAsn: 1.321 ± 0.04	0.628GlnPro: 0.628 ± 0.023	0.713GlnGln: 0.713 ± 0.025	1.052GlnArg: 1.052 ± 0.029	1.418GlnSer: 1.418 ± 0.033	0.899GlnThr: 0.899 ± 0.03	1.558GlnVal: 1.558 ± 0.038	0.274GlnTrp: 0.274 ± 0.016	1.053GlnTyr: 1.053 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	1.877ArgAla: 1.877 ± 0.039	0.382ArgCys: 0.382 ± 0.018	1.829ArgAsp: 1.829 ± 0.037	2.908ArgGlu: 2.908 ± 0.048	1.479ArgPhe: 1.479 ± 0.036	1.984ArgGly: 1.984 ± 0.045	0.462ArgHis: 0.462 ± 0.022	3.049ArgIle: 3.049 ± 0.051	2.985ArgLys: 2.985 ± 0.052	2.982ArgLeu: 2.982 ± 0.053	0.964ArgMet: 0.964 ± 0.031	2.031ArgAsn: 2.031 ± 0.046	0.856ArgPro: 0.856 ± 0.028	0.909ArgGln: 0.909 ± 0.024	1.43ArgArg: 1.43 ± 0.042	1.633ArgSer: 1.633 ± 0.039	1.552ArgThr: 1.552 ± 0.039	2.23ArgVal: 2.23 ± 0.044	0.273ArgTrp: 0.273 ± 0.015	1.538ArgTyr: 1.538 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.281SerAla: 3.281 ± 0.053	0.649SerCys: 0.649 ± 0.023	2.826SerAsp: 2.826 ± 0.047	4.152SerGlu: 4.152 ± 0.067	3.317SerPhe: 3.317 ± 0.054	4.168SerGly: 4.168 ± 0.056	0.982SerHis: 0.982 ± 0.031	6.578SerIle: 6.578 ± 0.085	5.878SerLys: 5.878 ± 0.077	6.363SerLeu: 6.363 ± 0.075	1.807SerMet: 1.807 ± 0.038	3.539SerAsn: 3.539 ± 0.062	1.819SerPro: 1.819 ± 0.039	1.695SerGln: 1.695 ± 0.039	2.03SerArg: 2.03 ± 0.042	4.418SerSer: 4.418 ± 0.059	3.174SerThr: 3.174 ± 0.046	3.753SerVal: 3.753 ± 0.052	0.516SerTrp: 0.516 ± 0.022	2.801SerTyr: 2.801 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	3.17ThrAla: 3.17 ± 0.051	0.446ThrCys: 0.446 ± 0.018	2.236ThrAsp: 2.236 ± 0.051	3.027ThrGlu: 3.027 ± 0.058	2.177ThrPhe: 2.177 ± 0.05	3.578ThrGly: 3.578 ± 0.059	0.814ThrHis: 0.814 ± 0.024	4.551ThrIle: 4.551 ± 0.064	3.49ThrLys: 3.49 ± 0.056	5.187ThrLeu: 5.187 ± 0.071	1.159ThrMet: 1.159 ± 0.028	2.465ThrAsn: 2.465 ± 0.049	1.901ThrPro: 1.901 ± 0.035	1.177ThrGln: 1.177 ± 0.038	1.489ThrArg: 1.489 ± 0.035	3.196ThrSer: 3.196 ± 0.056	2.543ThrThr: 2.543 ± 0.056	3.263ThrVal: 3.263 ± 0.063	0.421ThrTrp: 0.421 ± 0.018	1.806ThrTyr: 1.806 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	3.795ValAla: 3.795 ± 0.06	0.747ValCys: 0.747 ± 0.026	3.599ValAsp: 3.599 ± 0.057	4.425ValGlu: 4.425 ± 0.062	2.921ValPhe: 2.921 ± 0.052	4.036ValGly: 4.036 ± 0.07	0.847ValHis: 0.847 ± 0.028	6.274ValIle: 6.274 ± 0.071	5.546ValLys: 5.546 ± 0.08	6.18ValLeu: 6.18 ± 0.08	1.712ValMet: 1.712 ± 0.035	3.701ValAsn: 3.701 ± 0.059	2.111ValPro: 2.111 ± 0.046	1.519ValGln: 1.519 ± 0.039	1.984ValArg: 1.984 ± 0.044	4.211ValSer: 4.211 ± 0.065	3.272ValThr: 3.272 ± 0.055	4.404ValVal: 4.404 ± 0.072	0.375ValTrp: 0.375 ± 0.018	2.495ValTyr: 2.495 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.394TrpAla: 0.394 ± 0.02	0.115TrpCys: 0.115 ± 0.01	0.409TrpAsp: 0.409 ± 0.018	0.475TrpGlu: 0.475 ± 0.021	0.361TrpPhe: 0.361 ± 0.019	0.551TrpGly: 0.551 ± 0.023	0.133TrpHis: 0.133 ± 0.011	0.689TrpIle: 0.689 ± 0.024	0.578TrpLys: 0.578 ± 0.02	0.703TrpLeu: 0.703 ± 0.026	0.235TrpMet: 0.235 ± 0.014	0.502TrpAsn: 0.502 ± 0.019	0.22TrpPro: 0.22 ± 0.015	0.267TrpGln: 0.267 ± 0.018	0.332TrpArg: 0.332 ± 0.016	0.455TrpSer: 0.455 ± 0.019	0.337TrpThr: 0.337 ± 0.021	0.481TrpVal: 0.481 ± 0.022	0.102TrpTrp: 0.102 ± 0.008	0.292TrpTyr: 0.292 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.143TyrAla: 2.143 ± 0.042	0.515TyrCys: 0.515 ± 0.022	2.398TyrAsp: 2.398 ± 0.051	3.033TyrGlu: 3.033 ± 0.051	2.127TyrPhe: 2.127 ± 0.045	2.687TyrGly: 2.687 ± 0.049	0.641TyrHis: 0.641 ± 0.026	3.97TyrIle: 3.97 ± 0.068	3.842TyrLys: 3.842 ± 0.055	3.816TyrLeu: 3.816 ± 0.061	1.077TyrMet: 1.077 ± 0.03	2.888TyrAsn: 2.888 ± 0.049	1.378TyrPro: 1.378 ± 0.044	0.812TyrGln: 0.812 ± 0.027	1.503TyrArg: 1.503 ± 0.041	2.829TyrSer: 2.829 ± 0.05	2.078TyrThr: 2.078 ± 0.046	2.347TyrVal: 2.347 ± 0.043	0.362TyrTrp: 0.362 ± 0.018	1.934TyrTyr: 1.934 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3949 proteins (1223844 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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