Blastomyces parvus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae;

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2B7WR58|A0A2B7WR58_9EURO Acyl-CoA thioester hydrolase OS=Blastomyces parvus OX=2060905 GN=GX51_06442 PE=4 SV=1
MM1 pKa = 8.05ADD3 pKa = 3.37TDD5 pKa = 3.91GLSIYY10 pKa = 10.63DD11 pKa = 3.97EE12 pKa = 4.48IEE14 pKa = 4.49IEE16 pKa = 4.88DD17 pKa = 3.73MTYY20 pKa = 11.23DD21 pKa = 3.5PTLQIYY27 pKa = 9.13HH28 pKa = 5.87YY29 pKa = 8.86PCPCGDD35 pKa = 3.29RR36 pKa = 11.84FEE38 pKa = 5.81IGIADD43 pKa = 3.73LRR45 pKa = 11.84DD46 pKa = 3.61GEE48 pKa = 4.69EE49 pKa = 3.84IAVCPSCSLMVRR61 pKa = 11.84VIFDD65 pKa = 4.38LDD67 pKa = 3.88DD68 pKa = 4.05LPKK71 pKa = 11.0EE72 pKa = 4.26GDD74 pKa = 3.3TGAGAVEE81 pKa = 4.33ITAA84 pKa = 4.44

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2B7XNY1|A0A2B7XNY1_9EURO Uncharacterized protein OS=Blastomyces parvus OX=2060905 GN=GX51_00002 PE=4 SV=1
MM1 pKa = 7.6ASAGAFPSFRR11 pKa = 11.84VVWSGFKK18 pKa = 8.69PTRR21 pKa = 11.84RR22 pKa = 11.84GPILVRR28 pKa = 11.84VRR30 pKa = 11.84GSKK33 pKa = 7.59SQRR36 pKa = 11.84PGFVLEE42 pKa = 4.24SQLLPKK48 pKa = 10.34LLTVGEE54 pKa = 4.48VRR56 pKa = 11.84APRR59 pKa = 11.84YY60 pKa = 9.92DD61 pKa = 3.38DD62 pKa = 3.63GVRR65 pKa = 11.84IEE67 pKa = 4.33CPRR70 pKa = 11.84IRR72 pKa = 11.84RR73 pKa = 11.84VRR75 pKa = 11.84LSNASFQFDD84 pKa = 3.67QPCCQVAAAQSLLPIGARR102 pKa = 11.84ALVLAQLLSTWPRR115 pKa = 11.84LEE117 pKa = 4.01EE118 pKa = 4.18SLTGNLLAPTRR129 pKa = 11.84PDD131 pKa = 3.31YY132 pKa = 11.55ALDD135 pKa = 3.83AATSAEE141 pKa = 4.14QIKK144 pKa = 10.63

Molecular weight: 15.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8197

0

8197

4200543

30

5948

512.4

56.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.281 ± 0.021

1.165 ± 0.009

5.659 ± 0.02

6.215 ± 0.026

3.607 ± 0.017

6.851 ± 0.023

2.453 ± 0.012

4.978 ± 0.017

4.968 ± 0.023

8.778 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.063 ± 0.01

3.877 ± 0.012

6.452 ± 0.03

4.032 ± 0.022

6.319 ± 0.022

8.622 ± 0.033

5.93 ± 0.015

5.796 ± 0.02

1.301 ± 0.009

2.654 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski