Proteome-pI 2.0 - Proteome Isoelectric Point Database

Colobus angolensis palliatus (Peters Angolan colobus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 40241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A2K5IVM2|A0A2K5IVM2_COLAP Kinesin-like protein OS=Colobus angolensis palliatus OX=336983 PE=3 SV=1

MM1 pKa = 7.98DD2 pKa = 3.89VLFAAILAVPLILGQEE18 pKa = 4.28YY19 pKa = 10.37EE20 pKa = 4.08DD21 pKa = 3.9EE22 pKa = 4.23EE23 pKa = 4.66RR24 pKa = 11.84VGEE27 pKa = 4.02EE28 pKa = 3.7EE29 pKa = 4.92YY30 pKa = 11.13YY31 pKa = 10.48QVVYY35 pKa = 10.3YY36 pKa = 7.57YY37 pKa = 10.19TVTPSYY43 pKa = 11.45DD44 pKa = 3.41DD45 pKa = 4.42FSADD49 pKa = 3.3FTIDD53 pKa = 3.3YY54 pKa = 10.76SIFEE58 pKa = 4.24SEE60 pKa = 4.57DD61 pKa = 3.04RR62 pKa = 11.84LNRR65 pKa = 11.84LDD67 pKa = 4.3KK68 pKa = 10.73DD69 pKa = 3.46TTEE72 pKa = 4.3AVGTTISLEE81 pKa = 4.2TARR84 pKa = 11.84ADD86 pKa = 3.33HH87 pKa = 6.74PKK89 pKa = 9.96PVTVKK94 pKa = 10.35PVTTEE99 pKa = 3.9PSPDD103 pKa = 3.43LNDD106 pKa = 3.57AVSSLRR112 pKa = 11.84SPIPLLLSCAFVQAGMYY129 pKa = 9.95FMM131 pKa = 6.19

Molecular weight: 14.73 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A2K5JQH9|A0A2K5JQH9_COLAP Sodium/hydrogen exchanger OS=Colobus angolensis palliatus OX=336983 PE=3 SV=1

MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
20530	19711	40241	21242615	12	34264	527.9	58.93

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.762 ± 0.014	2.197 ± 0.012	4.899 ± 0.009	7.223 ± 0.019	3.64 ± 0.009	6.389 ± 0.019	2.597 ± 0.007	4.498 ± 0.012	5.946 ± 0.018	9.838 ± 0.022

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.182 ± 0.007	3.723 ± 0.009	6.084 ± 0.02	4.807 ± 0.014	5.558 ± 0.011	8.308 ± 0.018	5.369 ± 0.012	6.056 ± 0.014	1.208 ± 0.004	2.684 ± 0.007

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski