Levilactobacillus bambusae
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1886 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V1N1C4|A0A2V1N1C4_9LACO Uncharacterized protein OS=Levilactobacillus bambusae OX=2024736 GN=DCM90_04380 PE=4 SV=1
MM1 pKa = 7.75 FDD3 pKa = 3.23 NHH5 pKa = 5.87 HH6 pKa = 6.64 ARR8 pKa = 11.84 YY9 pKa = 9.68 ASYY12 pKa = 8.47 GTVAVLPGEE21 pKa = 4.58 MIDD24 pKa = 3.97 TVWYY28 pKa = 10.51 LIDD31 pKa = 4.09 QDD33 pKa = 4.2 LQGMFPLEE41 pKa = 4.11 NVLTFHH47 pKa = 7.46 LEE49 pKa = 3.98 AKK51 pKa = 9.69 DD52 pKa = 3.46 GKK54 pKa = 9.88 VAYY57 pKa = 9.99 RR58 pKa = 11.84 FQPTDD63 pKa = 3.58 LDD65 pKa = 4.36 DD66 pKa = 5.69 GIEE69 pKa = 3.98 LSATFDD75 pKa = 3.17 TDD77 pKa = 3.91 FPYY80 pKa = 10.91 SGDD83 pKa = 3.75 LPDD86 pKa = 5.24 TILAYY91 pKa = 10.38 DD92 pKa = 4.45 DD93 pKa = 5.05 GEE95 pKa = 4.36 SQIILLPAEE104 pKa = 4.25 ARR106 pKa = 11.84 NASFFF111 pKa = 4.09
Molecular weight: 12.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.859
IPC_protein 3.846
Toseland 3.63
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.668
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.973
Patrickios 1.914
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.863
Protein with the highest isoelectric point:
>tr|A0A2V1N393|A0A2V1N393_9LACO Uncharacterized protein OS=Levilactobacillus bambusae OX=2024736 GN=DCM90_03970 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.34 KK9 pKa = 7.4 RR10 pKa = 11.84 HH11 pKa = 5.68 RR12 pKa = 11.84 SRR14 pKa = 11.84 VHH16 pKa = 6.12 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 SVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1886
0
1886
563121
20
2101
298.6
33.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.242 ± 0.061
0.416 ± 0.013
6.147 ± 0.071
5.403 ± 0.062
4.041 ± 0.047
6.839 ± 0.051
2.303 ± 0.029
6.474 ± 0.053
5.239 ± 0.058
9.872 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.027
4.546 ± 0.041
3.84 ± 0.033
4.86 ± 0.058
4.432 ± 0.052
6.037 ± 0.074
6.658 ± 0.05
7.324 ± 0.048
1.134 ± 0.023
3.408 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here