Marinobacter sp. F3R11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter; unclassified Marinobacter

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3017 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366WJ66|A0A366WJ66_9ALTE Flagellar motor protein MotD OS=Marinobacter sp. F3R11 OX=2267231 GN=DS878_06825 PE=3 SV=1
MM1 pKa = 7.31AQQHH5 pKa = 5.71KK6 pKa = 10.74LKK8 pKa = 10.64FLVPALGLMMTACGGSDD25 pKa = 3.16NKK27 pKa = 10.33ISEE30 pKa = 4.15QADD33 pKa = 3.36DD34 pKa = 5.51GGASDD39 pKa = 4.8NDD41 pKa = 3.58YY42 pKa = 11.1SQLVIDD48 pKa = 5.58ARR50 pKa = 11.84TQTQYY55 pKa = 12.02LNLATGEE62 pKa = 4.45VVSSDD67 pKa = 3.93GDD69 pKa = 3.07WDD71 pKa = 3.86LAFNRR76 pKa = 11.84TSVLLNSGASGNGNVAGAMGDD97 pKa = 3.79EE98 pKa = 4.04QAEE101 pKa = 4.49FYY103 pKa = 11.02DD104 pKa = 4.56ADD106 pKa = 4.33GNPDD110 pKa = 3.29LNKK113 pKa = 10.06FVGATADD120 pKa = 3.86GEE122 pKa = 4.34LDD124 pKa = 3.42HH125 pKa = 7.14LKK127 pKa = 10.93DD128 pKa = 3.68SFPAPEE134 pKa = 4.03SWISDD139 pKa = 3.39DD140 pKa = 3.4VVYY143 pKa = 11.09AFGDD147 pKa = 3.45GWSVYY152 pKa = 10.79GDD154 pKa = 3.19GGVISEE160 pKa = 4.59VPDD163 pKa = 2.76IGYY166 pKa = 9.74LVRR169 pKa = 11.84SAEE172 pKa = 4.04GDD174 pKa = 3.25SYY176 pKa = 12.0ARR178 pKa = 11.84MRR180 pKa = 11.84IVDD183 pKa = 3.89FNFPTRR189 pKa = 11.84SGNGIEE195 pKa = 4.22SFNLEE200 pKa = 4.42FEE202 pKa = 4.51VQSAGTTQLSGTTINFTEE220 pKa = 4.25PLEE223 pKa = 4.17YY224 pKa = 10.61DD225 pKa = 3.58GGDD228 pKa = 3.02ACFDD232 pKa = 3.85FDD234 pKa = 4.02TNAVVDD240 pKa = 4.72CSSSDD245 pKa = 3.05TWDD248 pKa = 3.33VLIGFSGRR256 pKa = 11.84EE257 pKa = 3.87WYY259 pKa = 10.81LKK261 pKa = 9.69TNSGPSGAGQGGALGPIDD279 pKa = 3.8WSEE282 pKa = 3.77LSAYY286 pKa = 9.13TSATIDD292 pKa = 3.54SATGEE297 pKa = 4.56SLTQAYY303 pKa = 10.17ASDD306 pKa = 3.73STGGLFTDD314 pKa = 4.17NSWYY318 pKa = 10.52AYY320 pKa = 10.19NLQGAHH326 pKa = 6.89KK327 pKa = 9.12LWPNFRR333 pKa = 11.84VYY335 pKa = 10.97LIDD338 pKa = 4.67SDD340 pKa = 5.39SEE342 pKa = 4.16DD343 pKa = 3.3ASAPVYY349 pKa = 10.96AMQIINYY356 pKa = 8.84YY357 pKa = 9.78GADD360 pKa = 3.78GNSGQPEE367 pKa = 4.16IRR369 pKa = 11.84WKK371 pKa = 10.52EE372 pKa = 3.6ISLTTGEE379 pKa = 4.11NN380 pKa = 3.21

Molecular weight: 40.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366WVL1|A0A366WVL1_9ALTE Bifunctional protein GlmU OS=Marinobacter sp. F3R11 OX=2267231 GN=glmU PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.34VISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.41GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3017

0

3017

1025962

17

4222

340.1

37.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.694 ± 0.042

0.965 ± 0.016

5.67 ± 0.043

6.514 ± 0.047

3.804 ± 0.029

7.891 ± 0.041

2.148 ± 0.023

5.507 ± 0.037

3.775 ± 0.033

10.614 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.024

3.33 ± 0.031

4.63 ± 0.032

3.907 ± 0.03

6.164 ± 0.036

6.25 ± 0.039

5.23 ± 0.038

7.309 ± 0.038

1.343 ± 0.02

2.617 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski