Streptomyces sp. WAC00469

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5069 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A429N9R0|A0A429N9R0_9ACTN Glucanase OS=Streptomyces sp. WAC00469 OX=2487415 GN=EF917_14505 PE=3 SV=1
MM1 pKa = 7.87AEE3 pKa = 3.61QTYY6 pKa = 8.2TVSGMSCGHH15 pKa = 6.6CAASITEE22 pKa = 4.31EE23 pKa = 4.22VVEE26 pKa = 4.52VPGVTDD32 pKa = 3.12VDD34 pKa = 3.66VDD36 pKa = 4.17VPAGLVTVRR45 pKa = 11.84GEE47 pKa = 4.32SVDD50 pKa = 3.5ADD52 pKa = 3.57AVRR55 pKa = 11.84AAIVEE60 pKa = 3.99AGYY63 pKa = 10.71EE64 pKa = 4.24VTEE67 pKa = 4.42SMRR70 pKa = 11.84STVAA74 pKa = 3.13

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R9STD6|A0A3R9STD6_9ACTN Ribonuclease 3 OS=Streptomyces sp. WAC00469 OX=2487415 GN=rnc PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5069

0

5069

1630969

24

2240

321.8

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.642 ± 0.051

0.845 ± 0.009

6.044 ± 0.029

5.687 ± 0.039

2.62 ± 0.018

9.315 ± 0.036

2.408 ± 0.016

3.059 ± 0.025

2.117 ± 0.026

10.265 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.659 ± 0.013

1.613 ± 0.016

6.236 ± 0.032

2.871 ± 0.021

8.539 ± 0.041

4.755 ± 0.023

6.155 ± 0.029

8.661 ± 0.038

1.465 ± 0.014

2.044 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski