Amino acid dipepetide frequency for Streptomyces sp. WAC00469

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.037AlaAla: 22.037 ± 0.194
1.188AlaCys: 1.188 ± 0.028
8.337AlaAsp: 8.337 ± 0.085
8.876AlaGlu: 8.876 ± 0.106
3.369AlaPhe: 3.369 ± 0.048
13.146AlaGly: 13.146 ± 0.126
3.09AlaHis: 3.09 ± 0.044
3.077AlaIle: 3.077 ± 0.052
2.688AlaLys: 2.688 ± 0.053
14.381AlaLeu: 14.381 ± 0.109
2.342AlaMet: 2.342 ± 0.044
1.684AlaAsn: 1.684 ± 0.036
7.316AlaPro: 7.316 ± 0.096
4.061AlaGln: 4.061 ± 0.058
11.245AlaArg: 11.245 ± 0.112
5.689AlaSer: 5.689 ± 0.065
6.705AlaThr: 6.705 ± 0.075
12.695AlaVal: 12.695 ± 0.113
1.823AlaTrp: 1.823 ± 0.036
2.671AlaTyr: 2.671 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.231CysAla: 1.231 ± 0.033
0.118CysCys: 0.118 ± 0.01
0.502CysAsp: 0.502 ± 0.018
0.415CysGlu: 0.415 ± 0.015
0.246CysPhe: 0.246 ± 0.013
1.016CysGly: 1.016 ± 0.026
0.202CysHis: 0.202 ± 0.011
0.165CysIle: 0.165 ± 0.011
0.107CysLys: 0.107 ± 0.008
0.796CysLeu: 0.796 ± 0.022
0.132CysMet: 0.132 ± 0.009
0.131CysAsn: 0.131 ± 0.008
0.549CysPro: 0.549 ± 0.019
0.142CysGln: 0.142 ± 0.009
0.714CysArg: 0.714 ± 0.02
0.422CysSer: 0.422 ± 0.016
0.587CysThr: 0.587 ± 0.019
0.712CysVal: 0.712 ± 0.022
0.128CysTrp: 0.128 ± 0.009
0.137CysTyr: 0.137 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.811AspAla: 7.811 ± 0.083
0.435AspCys: 0.435 ± 0.018
3.803AspAsp: 3.803 ± 0.052
3.849AspGlu: 3.849 ± 0.06
1.59AspPhe: 1.59 ± 0.03
6.595AspGly: 6.595 ± 0.076
1.462AspHis: 1.462 ± 0.024
1.85AspIle: 1.85 ± 0.04
1.246AspLys: 1.246 ± 0.034
6.24AspLeu: 6.24 ± 0.071
0.769AspMet: 0.769 ± 0.022
0.88AspAsn: 0.88 ± 0.026
4.529AspPro: 4.529 ± 0.052
1.493AspGln: 1.493 ± 0.032
5.247AspArg: 5.247 ± 0.068
2.263AspSer: 2.263 ± 0.042
3.545AspThr: 3.545 ± 0.046
4.828AspVal: 4.828 ± 0.055
0.977AspTrp: 0.977 ± 0.029
1.026AspTyr: 1.026 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
7.403GluAla: 7.403 ± 0.109
0.337GluCys: 0.337 ± 0.012
3.044GluAsp: 3.044 ± 0.048
3.906GluGlu: 3.906 ± 0.077
1.316GluPhe: 1.316 ± 0.031
4.169GluGly: 4.169 ± 0.055
1.608GluHis: 1.608 ± 0.035
2.285GluIle: 2.285 ± 0.044
1.464GluLys: 1.464 ± 0.041
6.504GluLeu: 6.504 ± 0.083
0.868GluMet: 0.868 ± 0.024
1.016GluAsn: 1.016 ± 0.028
3.41GluPro: 3.41 ± 0.05
2.536GluGln: 2.536 ± 0.039
5.667GluArg: 5.667 ± 0.073
2.202GluSer: 2.202 ± 0.039
2.838GluThr: 2.838 ± 0.043
4.522GluVal: 4.522 ± 0.051
0.688GluTrp: 0.688 ± 0.021
1.088GluTyr: 1.088 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
3.531PheAla: 3.531 ± 0.052
0.267PheCys: 0.267 ± 0.012
1.924PheAsp: 1.924 ± 0.034
1.334PheGlu: 1.334 ± 0.03
0.843PhePhe: 0.843 ± 0.025
2.777PheGly: 2.777 ± 0.046
0.63PheHis: 0.63 ± 0.018
0.638PheIle: 0.638 ± 0.021
0.482PheLys: 0.482 ± 0.017
2.535PheLeu: 2.535 ± 0.052
0.354PheMet: 0.354 ± 0.015
0.511PheAsn: 0.511 ± 0.018
1.367PhePro: 1.367 ± 0.025
0.641PheGln: 0.641 ± 0.02
1.849PheArg: 1.849 ± 0.04
1.342PheSer: 1.342 ± 0.031
1.958PheThr: 1.958 ± 0.038
2.253PheVal: 2.253 ± 0.04
0.411PheTrp: 0.411 ± 0.015
0.555PheTyr: 0.555 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
10.775GlyAla: 10.775 ± 0.113
0.877GlyCys: 0.877 ± 0.024
5.121GlyAsp: 5.121 ± 0.056
4.961GlyGlu: 4.961 ± 0.055
2.729GlyPhe: 2.729 ± 0.046
8.413GlyGly: 8.413 ± 0.101
2.405GlyHis: 2.405 ± 0.041
3.373GlyIle: 3.373 ± 0.05
2.327GlyLys: 2.327 ± 0.042
9.218GlyLeu: 9.218 ± 0.084
1.863GlyMet: 1.863 ± 0.035
1.609GlyAsn: 1.609 ± 0.038
5.377GlyPro: 5.377 ± 0.076
2.622GlyGln: 2.622 ± 0.044
8.357GlyArg: 8.357 ± 0.075
5.011GlySer: 5.011 ± 0.07
6.764GlyThr: 6.764 ± 0.082
7.511GlyVal: 7.511 ± 0.074
1.665GlyTrp: 1.665 ± 0.035
2.169GlyTyr: 2.169 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.779HisAla: 2.779 ± 0.044
0.239HisCys: 0.239 ± 0.011
1.429HisAsp: 1.429 ± 0.026
1.264HisGlu: 1.264 ± 0.03
0.633HisPhe: 0.633 ± 0.021
2.683HisGly: 2.683 ± 0.049
0.697HisHis: 0.697 ± 0.022
0.704HisIle: 0.704 ± 0.021
0.375HisLys: 0.375 ± 0.015
2.499HisLeu: 2.499 ± 0.039
0.327HisMet: 0.327 ± 0.013
0.366HisAsn: 0.366 ± 0.015
1.94HisPro: 1.94 ± 0.035
0.649HisGln: 0.649 ± 0.023
2.413HisArg: 2.413 ± 0.046
0.951HisSer: 0.951 ± 0.023
1.47HisThr: 1.47 ± 0.028
1.778HisVal: 1.778 ± 0.036
0.396HisTrp: 0.396 ± 0.015
0.487HisTyr: 0.487 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
4.645IleAla: 4.645 ± 0.062
0.302IleCys: 0.302 ± 0.015
2.154IleAsp: 2.154 ± 0.041
1.93IleGlu: 1.93 ± 0.039
0.657IlePhe: 0.657 ± 0.02
3.389IleGly: 3.389 ± 0.051
0.597IleHis: 0.597 ± 0.019
0.773IleIle: 0.773 ± 0.025
0.754IleLys: 0.754 ± 0.024
2.302IleLeu: 2.302 ± 0.042
0.427IleMet: 0.427 ± 0.018
0.632IleAsn: 0.632 ± 0.021
1.709IlePro: 1.709 ± 0.035
0.671IleGln: 0.671 ± 0.02
2.271IleArg: 2.271 ± 0.035
1.511IleSer: 1.511 ± 0.029
2.163IleThr: 2.163 ± 0.043
2.822IleVal: 2.822 ± 0.048
0.35IleTrp: 0.35 ± 0.015
0.528IleTyr: 0.528 ± 0.02
0.0IleXaa: 0.0 ± 0.0
Lys
2.952LysAla: 2.952 ± 0.055
0.12LysCys: 0.12 ± 0.009
1.351LysAsp: 1.351 ± 0.036
1.238LysGlu: 1.238 ± 0.038
0.407LysPhe: 0.407 ± 0.018
1.815LysGly: 1.815 ± 0.041
0.416LysHis: 0.416 ± 0.017
0.894LysIle: 0.894 ± 0.026
0.919LysLys: 0.919 ± 0.035
1.963LysLeu: 1.963 ± 0.039
0.393LysMet: 0.393 ± 0.017
0.559LysAsn: 0.559 ± 0.022
1.25LysPro: 1.25 ± 0.031
0.74LysGln: 0.74 ± 0.022
1.453LysArg: 1.453 ± 0.033
0.988LysSer: 0.988 ± 0.028
1.242LysThr: 1.242 ± 0.035
1.839LysVal: 1.839 ± 0.036
0.223LysTrp: 0.223 ± 0.011
0.412LysTyr: 0.412 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.856LeuAla: 14.856 ± 0.118
0.902LeuCys: 0.902 ± 0.026
6.645LeuAsp: 6.645 ± 0.073
4.657LeuGlu: 4.657 ± 0.058
2.5LeuPhe: 2.5 ± 0.045
9.044LeuGly: 9.044 ± 0.096
2.457LeuHis: 2.457 ± 0.044
3.181LeuIle: 3.181 ± 0.046
2.069LeuLys: 2.069 ± 0.044
11.298LeuLeu: 11.298 ± 0.13
1.555LeuMet: 1.555 ± 0.03
1.53LeuAsn: 1.53 ± 0.033
6.652LeuPro: 6.652 ± 0.069
2.427LeuGln: 2.427 ± 0.04
9.064LeuArg: 9.064 ± 0.096
5.057LeuSer: 5.057 ± 0.059
6.764LeuThr: 6.764 ± 0.063
8.835LeuVal: 8.835 ± 0.086
1.253LeuTrp: 1.253 ± 0.032
1.902LeuTyr: 1.902 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
2.166MetAla: 2.166 ± 0.035
0.136MetCys: 0.136 ± 0.008
0.829MetAsp: 0.829 ± 0.02
0.697MetGlu: 0.697 ± 0.023
0.415MetPhe: 0.415 ± 0.018
1.243MetGly: 1.243 ± 0.029
0.371MetHis: 0.371 ± 0.016
0.662MetIle: 0.662 ± 0.024
0.387MetLys: 0.387 ± 0.014
1.626MetLeu: 1.626 ± 0.034
0.273MetMet: 0.273 ± 0.014
0.373MetAsn: 0.373 ± 0.016
1.059MetPro: 1.059 ± 0.022
0.446MetGln: 0.446 ± 0.017
1.416MetArg: 1.416 ± 0.03
1.264MetSer: 1.264 ± 0.024
1.504MetThr: 1.504 ± 0.031
1.221MetVal: 1.221 ± 0.03
0.19MetTrp: 0.19 ± 0.011
0.313MetTyr: 0.313 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.034AsnAla: 2.034 ± 0.039
0.137AsnCys: 0.137 ± 0.01
0.866AsnAsp: 0.866 ± 0.02
0.744AsnGlu: 0.744 ± 0.02
0.428AsnPhe: 0.428 ± 0.017
1.692AsnGly: 1.692 ± 0.04
0.37AsnHis: 0.37 ± 0.016
0.607AsnIle: 0.607 ± 0.021
0.395AsnLys: 0.395 ± 0.016
1.526AsnLeu: 1.526 ± 0.034
0.297AsnMet: 0.297 ± 0.014
0.375AsnAsn: 0.375 ± 0.018
1.287AsnPro: 1.287 ± 0.031
0.463AsnGln: 0.463 ± 0.02
1.183AsnArg: 1.183 ± 0.026
0.779AsnSer: 0.779 ± 0.024
0.991AsnThr: 0.991 ± 0.028
1.319AsnVal: 1.319 ± 0.032
0.261AsnTrp: 0.261 ± 0.014
0.375AsnTyr: 0.375 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
9.095ProAla: 9.095 ± 0.107
0.4ProCys: 0.4 ± 0.016
4.675ProAsp: 4.675 ± 0.056
4.485ProGlu: 4.485 ± 0.051
1.5ProPhe: 1.5 ± 0.03
6.805ProGly: 6.805 ± 0.088
1.516ProHis: 1.516 ± 0.037
1.139ProIle: 1.139 ± 0.029
1.128ProLys: 1.128 ± 0.032
5.376ProLeu: 5.376 ± 0.065
0.917ProMet: 0.917 ± 0.026
0.814ProAsn: 0.814 ± 0.024
3.734ProPro: 3.734 ± 0.081
1.984ProGln: 1.984 ± 0.046
4.39ProArg: 4.39 ± 0.056
3.21ProSer: 3.21 ± 0.051
2.994ProThr: 2.994 ± 0.049
5.833ProVal: 5.833 ± 0.062
0.905ProTrp: 0.905 ± 0.027
1.46ProTyr: 1.46 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.979GlnAla: 3.979 ± 0.05
0.177GlnCys: 0.177 ± 0.01
1.574GlnAsp: 1.574 ± 0.033
1.576GlnGlu: 1.576 ± 0.031
0.616GlnPhe: 0.616 ± 0.021
2.407GlnGly: 2.407 ± 0.047
0.708GlnHis: 0.708 ± 0.022
1.129GlnIle: 1.129 ± 0.03
0.66GlnLys: 0.66 ± 0.024
3.035GlnLeu: 3.035 ± 0.047
0.491GlnMet: 0.491 ± 0.017
0.497GlnAsn: 0.497 ± 0.02
1.798GlnPro: 1.798 ± 0.047
1.361GlnGln: 1.361 ± 0.037
2.488GlnArg: 2.488 ± 0.039
1.201GlnSer: 1.201 ± 0.025
1.367GlnThr: 1.367 ± 0.033
2.568GlnVal: 2.568 ± 0.039
0.449GlnTrp: 0.449 ± 0.018
0.631GlnTyr: 0.631 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
10.498ArgAla: 10.498 ± 0.109
0.683ArgCys: 0.683 ± 0.022
4.491ArgAsp: 4.491 ± 0.063
4.744ArgGlu: 4.744 ± 0.061
2.376ArgPhe: 2.376 ± 0.04
5.993ArgGly: 5.993 ± 0.065
2.316ArgHis: 2.316 ± 0.045
3.494ArgIle: 3.494 ± 0.045
1.644ArgLys: 1.644 ± 0.036
9.488ArgLeu: 9.488 ± 0.106
1.814ArgMet: 1.814 ± 0.036
1.288ArgAsn: 1.288 ± 0.03
5.603ArgPro: 5.603 ± 0.073
2.59ArgGln: 2.59 ± 0.046
8.911ArgArg: 8.911 ± 0.105
4.161ArgSer: 4.161 ± 0.054
5.945ArgThr: 5.945 ± 0.072
6.108ArgVal: 6.108 ± 0.069
1.418ArgTrp: 1.418 ± 0.031
1.825ArgTyr: 1.825 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
6.462SerAla: 6.462 ± 0.067
0.41SerCys: 0.41 ± 0.016
2.565SerAsp: 2.565 ± 0.043
2.222SerGlu: 2.222 ± 0.039
1.488SerPhe: 1.488 ± 0.03
5.54SerGly: 5.54 ± 0.063
0.996SerHis: 0.996 ± 0.027
1.242SerIle: 1.242 ± 0.027
0.911SerLys: 0.911 ± 0.029
4.511SerLeu: 4.511 ± 0.056
0.961SerMet: 0.961 ± 0.021
0.756SerAsn: 0.756 ± 0.022
3.077SerPro: 3.077 ± 0.047
1.131SerGln: 1.131 ± 0.028
3.696SerArg: 3.696 ± 0.058
2.662SerSer: 2.662 ± 0.046
2.849SerThr: 2.849 ± 0.04
4.114SerVal: 4.114 ± 0.05
0.838SerTrp: 0.838 ± 0.023
1.12SerTyr: 1.12 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
9.008ThrAla: 9.008 ± 0.087
0.489ThrCys: 0.489 ± 0.017
3.72ThrAsp: 3.72 ± 0.051
3.299ThrGlu: 3.299 ± 0.054
1.582ThrPhe: 1.582 ± 0.034
6.849ThrGly: 6.849 ± 0.076
1.237ThrHis: 1.237 ± 0.033
1.592ThrIle: 1.592 ± 0.038
1.098ThrLys: 1.098 ± 0.03
5.671ThrLeu: 5.671 ± 0.059
0.895ThrMet: 0.895 ± 0.025
0.871ThrAsn: 0.871 ± 0.024
4.182ThrPro: 4.182 ± 0.053
1.292ThrGln: 1.292 ± 0.034
4.213ThrArg: 4.213 ± 0.052
3.029ThrSer: 3.029 ± 0.053
3.787ThrThr: 3.787 ± 0.061
6.53ThrVal: 6.53 ± 0.072
0.902ThrTrp: 0.902 ± 0.026
1.305ThrTyr: 1.305 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.825ValAla: 10.825 ± 0.086
0.875ValCys: 0.875 ± 0.023
5.034ValAsp: 5.034 ± 0.056
4.722ValGlu: 4.722 ± 0.061
2.422ValPhe: 2.422 ± 0.044
6.312ValGly: 6.312 ± 0.07
2.222ValHis: 2.222 ± 0.045
2.752ValIle: 2.752 ± 0.045
1.771ValLys: 1.771 ± 0.04
9.888ValLeu: 9.888 ± 0.098
1.405ValMet: 1.405 ± 0.031
1.517ValAsn: 1.517 ± 0.037
5.726ValPro: 5.726 ± 0.064
2.218ValGln: 2.218 ± 0.039
7.613ValArg: 7.613 ± 0.072
4.102ValSer: 4.102 ± 0.05
5.892ValThr: 5.892 ± 0.071
8.545ValVal: 8.545 ± 0.097
1.141ValTrp: 1.141 ± 0.029
1.625ValTyr: 1.625 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.654TrpAla: 1.654 ± 0.034
0.166TrpCys: 0.166 ± 0.011
0.842TrpAsp: 0.842 ± 0.025
0.703TrpGlu: 0.703 ± 0.021
0.476TrpPhe: 0.476 ± 0.018
1.017TrpGly: 1.017 ± 0.029
0.379TrpHis: 0.379 ± 0.016
0.523TrpIle: 0.523 ± 0.019
0.352TrpLys: 0.352 ± 0.016
1.768TrpLeu: 1.768 ± 0.031
0.268TrpMet: 0.268 ± 0.012
0.356TrpAsn: 0.356 ± 0.015
0.752TrpPro: 0.752 ± 0.023
0.629TrpGln: 0.629 ± 0.021
1.303TrpArg: 1.303 ± 0.026
0.824TrpSer: 0.824 ± 0.023
0.985TrpThr: 0.985 ± 0.026
0.968TrpVal: 0.968 ± 0.027
0.322TrpTrp: 0.322 ± 0.014
0.367TrpTyr: 0.367 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.679TyrAla: 2.679 ± 0.047
0.193TyrCys: 0.193 ± 0.012
1.535TyrAsp: 1.535 ± 0.036
1.25TyrGlu: 1.25 ± 0.029
0.609TyrPhe: 0.609 ± 0.019
2.243TyrGly: 2.243 ± 0.035
0.4TyrHis: 0.4 ± 0.017
0.506TyrIle: 0.506 ± 0.017
0.395TyrLys: 0.395 ± 0.016
2.024TyrLeu: 2.024 ± 0.04
0.24TyrMet: 0.24 ± 0.011
0.364TyrAsn: 0.364 ± 0.015
1.04TyrPro: 1.04 ± 0.023
0.616TyrGln: 0.616 ± 0.019
1.895TyrArg: 1.895 ± 0.042
0.885TyrSer: 0.885 ± 0.028
1.2TyrThr: 1.2 ± 0.031
1.605TyrVal: 1.605 ± 0.03
0.316TyrTrp: 0.316 ± 0.013
0.445TyrTyr: 0.445 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5069 proteins (1630969 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski