Xanthomonas phage XPV1 
Average proteome isoelectric point is 6.26 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A3S7I6B3|A0A3S7I6B3_9CAUD Uncharacterized protein OS=Xanthomonas phage XPV1 OX=2099860 PE=4 SV=1 
MM1 pKa = 7.73  IPDD4 pKa = 3.69  WDD6 pKa = 5.04  DD7 pKa = 3.86  APDD10 pKa = 3.48  WAQYY14 pKa = 9.22  AACDD18 pKa = 4.09  YY19 pKa = 11.07  NGVWYY24 pKa = 8.83  WYY26 pKa = 9.63  DD27 pKa = 3.46  AEE29 pKa = 4.92  PYY31 pKa = 9.28  IHH33 pKa = 7.58  PSGVWAWYY41 pKa = 9.41  GVRR44 pKa = 11.84  AEE46 pKa = 5.07  AINPITVDD54 pKa = 2.82  WTDD57 pKa = 2.83  SKK59 pKa = 10.75  MRR61 pKa = 11.84  RR62 pKa = 11.84  PP63 pKa = 3.67   
 Molecular weight: 7.48 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.896 
IPC2_protein 4.075 
IPC_protein 3.986 
Toseland    3.77 
ProMoST     4.19 
Dawson      3.999 
Bjellqvist  4.164 
Wikipedia   3.986 
Rodwell     3.821 
Grimsley    3.681 
Solomon     3.986 
Lehninger   3.935 
Nozaki      4.139 
DTASelect   4.406 
Thurlkill   3.846 
EMBOSS      3.986 
Sillero     4.113 
Patrickios  0.477 
IPC_peptide 3.973 
IPC2_peptide  4.088 
IPC2.peptide.svr19  4.009 
 Protein with the highest isoelectric point: 
>tr|A0A3S7I6I4|A0A3S7I6I4_9CAUD DNA polymerase I OS=Xanthomonas phage XPV1 OX=2099860 PE=4 SV=1 
MM1 pKa = 7.57  SRR3 pKa = 11.84  QTNSTTKK10 pKa = 10.33  RR11 pKa = 11.84  NPRR14 pKa = 11.84  DD15 pKa = 3.5  FLDD18 pKa = 4.41  ADD20 pKa = 3.84  ATPVPTWTVRR30 pKa = 11.84  EE31 pKa = 4.08  LVDD34 pKa = 3.54  AVGIEE39 pKa = 4.11  TAAQLWQISTNNARR53 pKa = 11.84  QVRR56 pKa = 11.84  HH57 pKa = 6.34  RR58 pKa = 11.84  GAASLEE64 pKa = 4.05  RR65 pKa = 11.84  MQVLHH70 pKa = 5.96  AHH72 pKa = 6.11  IRR74 pKa = 11.84  TNEE77 pKa = 3.3  AHH79 pKa = 5.88  YY80 pKa = 10.86  RR81 pKa = 11.84  EE82 pKa = 4.44  TLVTIYY88 pKa = 9.8  STGAFRR94 pKa = 11.84  RR95 pKa = 11.84  QSS97 pKa = 3.0   
 Molecular weight: 11.08 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.319 
IPC2_protein 9.589 
IPC_protein 10.833 
Toseland    10.672 
ProMoST     10.847 
Dawson      10.789 
Bjellqvist  10.672 
Wikipedia   11.155 
Rodwell     10.599 
Grimsley    10.862 
Solomon     11.082 
Lehninger   11.008 
Nozaki      10.657 
DTASelect   10.672 
Thurlkill   10.701 
EMBOSS      11.125 
Sillero     10.745 
Patrickios  10.423 
IPC_peptide 11.067 
IPC2_peptide  9.926 
IPC2.peptide.svr19  8.717 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        77 
 
        
        0
 
        
        77 
         
        13923
 
        47
 
        610
 
        180.8
 
        19.63
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        12.368 ± 0.395
0.776 ± 0.094
 
        6.392 ± 0.315
4.511 ± 0.296
 
        2.887 ± 0.175
7.814 ± 0.535
 
        1.451 ± 0.141
4.511 ± 0.208
       
        3.053 ± 0.234
8.482 ± 0.32
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.277 ± 0.133
3.369 ± 0.21
 
        5.624 ± 0.302
4.23 ± 0.231
 
        6.572 ± 0.329
6.241 ± 0.216
 
        7.089 ± 0.331
7.441 ± 0.277
       
        1.731 ± 0.157
3.182 ± 0.161
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here