Xanthomonas phage XPV1
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S7I6B3|A0A3S7I6B3_9CAUD Uncharacterized protein OS=Xanthomonas phage XPV1 OX=2099860 PE=4 SV=1
MM1 pKa = 7.73 IPDD4 pKa = 3.69 WDD6 pKa = 5.04 DD7 pKa = 3.86 APDD10 pKa = 3.48 WAQYY14 pKa = 9.22 AACDD18 pKa = 4.09 YY19 pKa = 11.07 NGVWYY24 pKa = 8.83 WYY26 pKa = 9.63 DD27 pKa = 3.46 AEE29 pKa = 4.92 PYY31 pKa = 9.28 IHH33 pKa = 7.58 PSGVWAWYY41 pKa = 9.41 GVRR44 pKa = 11.84 AEE46 pKa = 5.07 AINPITVDD54 pKa = 2.82 WTDD57 pKa = 2.83 SKK59 pKa = 10.75 MRR61 pKa = 11.84 RR62 pKa = 11.84 PP63 pKa = 3.67
Molecular weight: 7.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.896
IPC2_protein 4.075
IPC_protein 3.986
Toseland 3.77
ProMoST 4.19
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.986
Rodwell 3.821
Grimsley 3.681
Solomon 3.986
Lehninger 3.935
Nozaki 4.139
DTASelect 4.406
Thurlkill 3.846
EMBOSS 3.986
Sillero 4.113
Patrickios 0.477
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 4.009
Protein with the highest isoelectric point:
>tr|A0A3S7I6I4|A0A3S7I6I4_9CAUD DNA polymerase I OS=Xanthomonas phage XPV1 OX=2099860 PE=4 SV=1
MM1 pKa = 7.57 SRR3 pKa = 11.84 QTNSTTKK10 pKa = 10.33 RR11 pKa = 11.84 NPRR14 pKa = 11.84 DD15 pKa = 3.5 FLDD18 pKa = 4.41 ADD20 pKa = 3.84 ATPVPTWTVRR30 pKa = 11.84 EE31 pKa = 4.08 LVDD34 pKa = 3.54 AVGIEE39 pKa = 4.11 TAAQLWQISTNNARR53 pKa = 11.84 QVRR56 pKa = 11.84 HH57 pKa = 6.34 RR58 pKa = 11.84 GAASLEE64 pKa = 4.05 RR65 pKa = 11.84 MQVLHH70 pKa = 5.96 AHH72 pKa = 6.11 IRR74 pKa = 11.84 TNEE77 pKa = 3.3 AHH79 pKa = 5.88 YY80 pKa = 10.86 RR81 pKa = 11.84 EE82 pKa = 4.44 TLVTIYY88 pKa = 9.8 STGAFRR94 pKa = 11.84 RR95 pKa = 11.84 QSS97 pKa = 3.0
Molecular weight: 11.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.589
IPC_protein 10.833
Toseland 10.672
ProMoST 10.847
Dawson 10.789
Bjellqvist 10.672
Wikipedia 11.155
Rodwell 10.599
Grimsley 10.862
Solomon 11.082
Lehninger 11.008
Nozaki 10.657
DTASelect 10.672
Thurlkill 10.701
EMBOSS 11.125
Sillero 10.745
Patrickios 10.423
IPC_peptide 11.067
IPC2_peptide 9.926
IPC2.peptide.svr19 8.717
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
13923
47
610
180.8
19.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.368 ± 0.395
0.776 ± 0.094
6.392 ± 0.315
4.511 ± 0.296
2.887 ± 0.175
7.814 ± 0.535
1.451 ± 0.141
4.511 ± 0.208
3.053 ± 0.234
8.482 ± 0.32
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.277 ± 0.133
3.369 ± 0.21
5.624 ± 0.302
4.23 ± 0.231
6.572 ± 0.329
6.241 ± 0.216
7.089 ± 0.331
7.441 ± 0.277
1.731 ± 0.157
3.182 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here