Lachnospiraceae bacterium MD335

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9LBG8|R9LBG8_9FIRM Diguanylate cyclase (GGDEF) domain-containing protein OS=Lachnospiraceae bacterium MD335 OX=1235793 GN=C809_00037 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 10.01KK3 pKa = 10.32RR4 pKa = 11.84KK5 pKa = 8.19ITALLMSLVMVCSLTACGGKK25 pKa = 8.34DD26 pKa = 3.07TTTTQPEE33 pKa = 4.38PAPAPAPADD42 pKa = 3.41NAATQDD48 pKa = 3.41TAKK51 pKa = 10.53QDD53 pKa = 3.71TAEE56 pKa = 4.31PAASGDD62 pKa = 3.51AAQAADD68 pKa = 3.56ITLWTYY74 pKa = 10.79PVGSWGDD81 pKa = 3.28AATVDD86 pKa = 5.17DD87 pKa = 5.27MIANFNAAYY96 pKa = 9.4PDD98 pKa = 3.06IHH100 pKa = 6.03VTVEE104 pKa = 3.71YY105 pKa = 10.93LDD107 pKa = 3.8YY108 pKa = 11.48TNGDD112 pKa = 3.66DD113 pKa = 5.12QINTAIEE120 pKa = 4.27GNQAPDD126 pKa = 3.07IVLEE130 pKa = 4.45GPEE133 pKa = 3.97RR134 pKa = 11.84LVANWGARR142 pKa = 11.84GLMVDD147 pKa = 5.69LADD150 pKa = 5.15LWTDD154 pKa = 3.38DD155 pKa = 4.33AKK157 pKa = 11.28AAIYY161 pKa = 10.52DD162 pKa = 4.05SVEE165 pKa = 4.13NACKK169 pKa = 10.56SSDD172 pKa = 3.39GVFYY176 pKa = 9.91EE177 pKa = 4.79YY178 pKa = 9.82PLCMTAHH185 pKa = 6.13TMAINRR191 pKa = 11.84DD192 pKa = 3.06IFEE195 pKa = 4.45AAGALQYY202 pKa = 11.12LDD204 pKa = 4.29EE205 pKa = 5.21EE206 pKa = 4.66KK207 pKa = 10.62GTWTTEE213 pKa = 3.67NFQKK217 pKa = 10.68AVQAVYY223 pKa = 11.15DD224 pKa = 3.79NGQQNVGAVYY234 pKa = 10.63CSGQGGDD241 pKa = 2.96QGTRR245 pKa = 11.84ALINNLYY252 pKa = 10.63GGTFTNPEE260 pKa = 4.01HH261 pKa = 6.41TEE263 pKa = 3.87YY264 pKa = 10.23TANSPEE270 pKa = 4.19NIKK273 pKa = 10.73ALEE276 pKa = 4.21LLKK279 pKa = 11.38SMDD282 pKa = 4.81GINFDD287 pKa = 3.76ASIAGGDD294 pKa = 4.01EE295 pKa = 4.08VNMFCNGTFAMAFCWNATQEE315 pKa = 4.56KK316 pKa = 10.3NNQEE320 pKa = 3.74QGNINFDD327 pKa = 4.2VLPMAFPSEE336 pKa = 4.07NGEE339 pKa = 3.95PQLCGGIWGFGIFDD353 pKa = 4.06NGDD356 pKa = 3.41AAKK359 pKa = 10.07IAASKK364 pKa = 9.63TFIDD368 pKa = 5.9FMANDD373 pKa = 3.92PTQAPLSVQASNFWPVKK390 pKa = 10.27DD391 pKa = 4.76LGNIYY396 pKa = 10.84DD397 pKa = 4.52GDD399 pKa = 4.25DD400 pKa = 4.06LMSEE404 pKa = 4.23YY405 pKa = 11.26AKK407 pKa = 10.3FIPYY411 pKa = 9.15MGDD414 pKa = 3.18YY415 pKa = 8.96YY416 pKa = 11.11QVVGGWAEE424 pKa = 4.84ARR426 pKa = 11.84TAWWNMLQQIGSGTDD441 pKa = 2.74VATAVEE447 pKa = 4.49EE448 pKa = 4.5FVTTANAAAAKK459 pKa = 10.05

Molecular weight: 49.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9KRQ4|R9KRQ4_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium MD335 OX=1235793 GN=C809_01967 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTKK25 pKa = 10.26AGRR28 pKa = 11.84KK29 pKa = 8.26VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4614

0

4614

1393863

20

4709

302.1

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.719 ± 0.044

1.516 ± 0.015

5.857 ± 0.03

7.669 ± 0.038

4.08 ± 0.024

6.707 ± 0.03

1.633 ± 0.018

7.242 ± 0.037

6.989 ± 0.038

8.571 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.039 ± 0.023

4.734 ± 0.031

2.996 ± 0.023

3.416 ± 0.022

4.468 ± 0.037

5.769 ± 0.031

5.56 ± 0.047

6.687 ± 0.031

0.937 ± 0.012

4.411 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski