Amino acid dipepetide frequency for Lachnospiraceae bacterium MD335

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.776AlaAla: 7.776 ± 0.111	1.099AlaCys: 1.099 ± 0.03	5.259AlaAsp: 5.259 ± 0.075	5.702AlaGlu: 5.702 ± 0.074	3.214AlaPhe: 3.214 ± 0.056	5.876AlaGly: 5.876 ± 0.078	1.052AlaHis: 1.052 ± 0.029	4.698AlaIle: 4.698 ± 0.068	5.099AlaLys: 5.099 ± 0.066	6.981AlaLeu: 6.981 ± 0.078	2.285AlaMet: 2.285 ± 0.042	2.837AlaAsn: 2.837 ± 0.047	2.015AlaPro: 2.015 ± 0.042	2.949AlaGln: 2.949 ± 0.051	2.815AlaArg: 2.815 ± 0.045	3.915AlaSer: 3.915 ± 0.058	3.135AlaThr: 3.135 ± 0.057	6.728AlaVal: 6.728 ± 0.083	0.642AlaTrp: 0.642 ± 0.025	3.116AlaTyr: 3.116 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.086CysAla: 1.086 ± 0.029	0.29CysCys: 0.29 ± 0.017	0.857CysAsp: 0.857 ± 0.027	0.961CysGlu: 0.961 ± 0.031	0.726CysPhe: 0.726 ± 0.025	1.423CysGly: 1.423 ± 0.04	0.314CysHis: 0.314 ± 0.015	1.104CysIle: 1.104 ± 0.03	0.899CysLys: 0.899 ± 0.029	1.154CysLeu: 1.154 ± 0.031	0.466CysMet: 0.466 ± 0.02	0.647CysAsn: 0.647 ± 0.021	0.555CysPro: 0.555 ± 0.02	0.411CysGln: 0.411 ± 0.018	0.763CysArg: 0.763 ± 0.027	0.865CysSer: 0.865 ± 0.026	0.745CysThr: 0.745 ± 0.027	1.108CysVal: 1.108 ± 0.033	0.113CysTrp: 0.113 ± 0.008	0.669CysTyr: 0.669 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	4.8AspAla: 4.8 ± 0.058	0.886AspCys: 0.886 ± 0.026	3.203AspAsp: 3.203 ± 0.056	4.825AspGlu: 4.825 ± 0.068	2.751AspPhe: 2.751 ± 0.055	4.504AspGly: 4.504 ± 0.074	0.649AspHis: 0.649 ± 0.027	4.814AspIle: 4.814 ± 0.065	4.052AspLys: 4.052 ± 0.063	4.297AspLeu: 4.297 ± 0.044	2.03AspMet: 2.03 ± 0.039	2.663AspAsn: 2.663 ± 0.049	1.323AspPro: 1.323 ± 0.036	0.969AspGln: 0.969 ± 0.025	2.565AspArg: 2.565 ± 0.047	3.213AspSer: 3.213 ± 0.05	3.576AspThr: 3.576 ± 0.063	3.804AspVal: 3.804 ± 0.061	0.628AspTrp: 0.628 ± 0.022	3.021AspTyr: 3.021 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	5.433GluAla: 5.433 ± 0.08	0.954GluCys: 0.954 ± 0.025	4.046GluAsp: 4.046 ± 0.062	6.788GluGlu: 6.788 ± 0.089	2.487GluPhe: 2.487 ± 0.045	4.229GluGly: 4.229 ± 0.069	1.398GluHis: 1.398 ± 0.034	5.701GluIle: 5.701 ± 0.077	6.531GluLys: 6.531 ± 0.079	6.896GluLeu: 6.896 ± 0.079	2.319GluMet: 2.319 ± 0.044	4.522GluAsn: 4.522 ± 0.062	1.903GluPro: 1.903 ± 0.046	3.393GluGln: 3.393 ± 0.056	3.735GluArg: 3.735 ± 0.067	3.509GluSer: 3.509 ± 0.055	4.455GluThr: 4.455 ± 0.075	4.157GluVal: 4.157 ± 0.061	0.783GluTrp: 0.783 ± 0.025	3.454GluTyr: 3.454 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.035PheAla: 3.035 ± 0.052	0.861PheCys: 0.861 ± 0.029	2.713PheAsp: 2.713 ± 0.054	2.777PheGlu: 2.777 ± 0.043	1.819PhePhe: 1.819 ± 0.04	2.863PheGly: 2.863 ± 0.049	0.806PheHis: 0.806 ± 0.029	2.641PheIle: 2.641 ± 0.048	1.888PheLys: 1.888 ± 0.034	3.893PheLeu: 3.893 ± 0.069	1.264PheMet: 1.264 ± 0.033	1.602PheAsn: 1.602 ± 0.038	1.297PhePro: 1.297 ± 0.034	1.323PheGln: 1.323 ± 0.032	1.792PheArg: 1.792 ± 0.039	2.863PheSer: 2.863 ± 0.055	2.332PheThr: 2.332 ± 0.047	2.743PheVal: 2.743 ± 0.046	0.438PheTrp: 0.438 ± 0.021	1.852PheTyr: 1.852 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.787GlyAla: 4.787 ± 0.086	1.149GlyCys: 1.149 ± 0.033	3.429GlyAsp: 3.429 ± 0.052	4.61GlyGlu: 4.61 ± 0.065	2.966GlyPhe: 2.966 ± 0.053	4.373GlyGly: 4.373 ± 0.087	1.09GlyHis: 1.09 ± 0.035	5.881GlyIle: 5.881 ± 0.074	5.298GlyLys: 5.298 ± 0.079	5.242GlyLeu: 5.242 ± 0.068	2.429GlyMet: 2.429 ± 0.041	3.382GlyAsn: 3.382 ± 0.056	0.868GlyPro: 0.868 ± 0.036	1.995GlyGln: 1.995 ± 0.037	2.99GlyArg: 2.99 ± 0.057	3.866GlySer: 3.866 ± 0.062	4.191GlyThr: 4.191 ± 0.076	4.564GlyVal: 4.564 ± 0.054	0.644GlyTrp: 0.644 ± 0.022	3.313GlyTyr: 3.313 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.093HisAla: 1.093 ± 0.035	0.281HisCys: 0.281 ± 0.016	0.918HisAsp: 0.918 ± 0.028	1.093HisGlu: 1.093 ± 0.031	0.814HisPhe: 0.814 ± 0.026	1.086HisGly: 1.086 ± 0.031	0.347HisHis: 0.347 ± 0.019	1.356HisIle: 1.356 ± 0.035	0.98HisLys: 0.98 ± 0.027	1.323HisLeu: 1.323 ± 0.033	0.53HisMet: 0.53 ± 0.02	0.756HisAsn: 0.756 ± 0.026	0.702HisPro: 0.702 ± 0.021	0.496HisGln: 0.496 ± 0.019	0.717HisArg: 0.717 ± 0.026	0.932HisSer: 0.932 ± 0.027	0.97HisThr: 0.97 ± 0.028	1.024HisVal: 1.024 ± 0.028	0.145HisTrp: 0.145 ± 0.01	0.761HisTyr: 0.761 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.798IleAla: 5.798 ± 0.084	1.278IleCys: 1.278 ± 0.033	4.212IleAsp: 4.212 ± 0.06	4.962IleGlu: 4.962 ± 0.067	2.748IlePhe: 2.748 ± 0.049	4.749IleGly: 4.749 ± 0.067	1.244IleHis: 1.244 ± 0.032	4.913IleIle: 4.913 ± 0.064	4.627IleLys: 4.627 ± 0.072	6.313IleLeu: 6.313 ± 0.086	2.111IleMet: 2.111 ± 0.044	3.221IleAsn: 3.221 ± 0.059	2.792IlePro: 2.792 ± 0.048	2.269IleGln: 2.269 ± 0.042	3.541IleArg: 3.541 ± 0.059	4.882IleSer: 4.882 ± 0.065	4.401IleThr: 4.401 ± 0.074	4.813IleVal: 4.813 ± 0.055	0.633IleTrp: 0.633 ± 0.024	2.913IleTyr: 2.913 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	5.224LysAla: 5.224 ± 0.079	0.786LysCys: 0.786 ± 0.027	3.958LysAsp: 3.958 ± 0.054	6.36LysGlu: 6.36 ± 0.077	1.878LysPhe: 1.878 ± 0.04	4.317LysGly: 4.317 ± 0.059	1.073LysHis: 1.073 ± 0.028	4.932LysIle: 4.932 ± 0.07	6.096LysLys: 6.096 ± 0.08	5.472LysLeu: 5.472 ± 0.076	2.076LysMet: 2.076 ± 0.042	4.171LysAsn: 4.171 ± 0.061	2.093LysPro: 2.093 ± 0.039	2.618LysGln: 2.618 ± 0.044	3.388LysArg: 3.388 ± 0.063	3.741LysSer: 3.741 ± 0.058	4.045LysThr: 4.045 ± 0.062	4.019LysVal: 4.019 ± 0.062	0.699LysTrp: 0.699 ± 0.026	2.944LysTyr: 2.944 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	6.412LeuAla: 6.412 ± 0.075	1.509LeuCys: 1.509 ± 0.037	5.006LeuAsp: 5.006 ± 0.068	6.316LeuGlu: 6.316 ± 0.083	3.835LeuPhe: 3.835 ± 0.065	5.207LeuGly: 5.207 ± 0.055	1.447LeuHis: 1.447 ± 0.031	5.615LeuIle: 5.615 ± 0.075	5.874LeuLys: 5.874 ± 0.062	8.101LeuLeu: 8.101 ± 0.115	2.543LeuMet: 2.543 ± 0.049	3.81LeuAsn: 3.81 ± 0.052	3.122LeuPro: 3.122 ± 0.042	2.787LeuGln: 2.787 ± 0.051	3.739LeuArg: 3.739 ± 0.061	6.184LeuSer: 6.184 ± 0.075	4.925LeuThr: 4.925 ± 0.07	4.925LeuVal: 4.925 ± 0.066	0.788LeuTrp: 0.788 ± 0.027	3.567LeuTyr: 3.567 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.348MetAla: 2.348 ± 0.048	0.387MetCys: 0.387 ± 0.015	1.804MetAsp: 1.804 ± 0.035	2.652MetGlu: 2.652 ± 0.051	0.989MetPhe: 0.989 ± 0.03	1.945MetGly: 1.945 ± 0.037	0.446MetHis: 0.446 ± 0.017	2.066MetIle: 2.066 ± 0.041	2.393MetLys: 2.393 ± 0.04	2.705MetLeu: 2.705 ± 0.047	0.827MetMet: 0.827 ± 0.022	1.602MetAsn: 1.602 ± 0.035	1.143MetPro: 1.143 ± 0.029	1.214MetGln: 1.214 ± 0.027	1.409MetArg: 1.409 ± 0.032	1.746MetSer: 1.746 ± 0.034	1.764MetThr: 1.764 ± 0.039	1.733MetVal: 1.733 ± 0.034	0.23MetTrp: 0.23 ± 0.013	0.987MetTyr: 0.987 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.909AsnAla: 3.909 ± 0.057	0.651AsnCys: 0.651 ± 0.024	2.503AsnAsp: 2.503 ± 0.046	3.289AsnGlu: 3.289 ± 0.052	1.725AsnPhe: 1.725 ± 0.034	3.924AsnGly: 3.924 ± 0.063	0.811AsnHis: 0.811 ± 0.027	3.72AsnIle: 3.72 ± 0.049	2.835AsnLys: 2.835 ± 0.045	3.664AsnLeu: 3.664 ± 0.055	1.515AsnMet: 1.515 ± 0.032	2.289AsnAsn: 2.289 ± 0.056	2.004AsnPro: 2.004 ± 0.042	1.479AsnGln: 1.479 ± 0.031	2.253AsnArg: 2.253 ± 0.042	2.549AsnSer: 2.549 ± 0.049	2.622AsnThr: 2.622 ± 0.049	3.127AsnVal: 3.127 ± 0.06	0.42AsnTrp: 0.42 ± 0.019	2.05AsnTyr: 2.05 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	2.37ProAla: 2.37 ± 0.039	0.413ProCys: 0.413 ± 0.018	2.182ProAsp: 2.182 ± 0.045	2.716ProGlu: 2.716 ± 0.057	1.432ProPhe: 1.432 ± 0.034	1.522ProGly: 1.522 ± 0.039	0.526ProHis: 0.526 ± 0.02	2.017ProIle: 2.017 ± 0.039	2.15ProLys: 2.15 ± 0.045	2.345ProLeu: 2.345 ± 0.048	0.819ProMet: 0.819 ± 0.025	1.324ProAsn: 1.324 ± 0.036	0.754ProPro: 0.754 ± 0.026	1.221ProGln: 1.221 ± 0.031	0.913ProArg: 0.913 ± 0.027	1.578ProSer: 1.578 ± 0.039	1.363ProThr: 1.363 ± 0.044	2.71ProVal: 2.71 ± 0.045	0.237ProTrp: 0.237 ± 0.016	1.363ProTyr: 1.363 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	2.482GlnAla: 2.482 ± 0.047	0.448GlnCys: 0.448 ± 0.02	1.652GlnAsp: 1.652 ± 0.034	3.023GlnGlu: 3.023 ± 0.052	1.238GlnPhe: 1.238 ± 0.027	1.992GlnGly: 1.992 ± 0.04	0.463GlnHis: 0.463 ± 0.019	2.569GlnIle: 2.569 ± 0.045	2.853GlnLys: 2.853 ± 0.043	2.876GlnLeu: 2.876 ± 0.048	1.147GlnMet: 1.147 ± 0.028	1.963GlnAsn: 1.963 ± 0.039	0.969GlnPro: 0.969 ± 0.032	1.34GlnGln: 1.34 ± 0.039	1.538GlnArg: 1.538 ± 0.035	1.823GlnSer: 1.823 ± 0.043	1.999GlnThr: 1.999 ± 0.046	1.831GlnVal: 1.831 ± 0.038	0.349GlnTrp: 0.349 ± 0.017	1.602GlnTyr: 1.602 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.889ArgAla: 2.889 ± 0.049	0.612ArgCys: 0.612 ± 0.022	2.271ArgAsp: 2.271 ± 0.051	3.858ArgGlu: 3.858 ± 0.061	1.971ArgPhe: 1.971 ± 0.046	2.413ArgGly: 2.413 ± 0.051	0.801ArgHis: 0.801 ± 0.025	3.671ArgIle: 3.671 ± 0.058	3.508ArgLys: 3.508 ± 0.057	4.028ArgLeu: 4.028 ± 0.073	1.603ArgMet: 1.603 ± 0.035	2.161ArgAsn: 2.161 ± 0.043	1.095ArgPro: 1.095 ± 0.034	1.841ArgGln: 1.841 ± 0.043	2.408ArgArg: 2.408 ± 0.053	2.086ArgSer: 2.086 ± 0.043	2.336ArgThr: 2.336 ± 0.043	2.658ArgVal: 2.658 ± 0.049	0.435ArgTrp: 0.435 ± 0.02	2.032ArgTyr: 2.032 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	4.639SerAla: 4.639 ± 0.064	0.788SerCys: 0.788 ± 0.025	3.692SerAsp: 3.692 ± 0.064	4.264SerGlu: 4.264 ± 0.062	2.674SerPhe: 2.674 ± 0.051	4.729SerGly: 4.729 ± 0.072	0.924SerHis: 0.924 ± 0.03	4.145SerIle: 4.145 ± 0.065	3.566SerLys: 3.566 ± 0.052	4.777SerLeu: 4.777 ± 0.066	1.702SerMet: 1.702 ± 0.039	2.436SerAsn: 2.436 ± 0.043	1.537SerPro: 1.537 ± 0.034	1.827SerGln: 1.827 ± 0.041	2.535SerArg: 2.535 ± 0.049	3.37SerSer: 3.37 ± 0.064	2.675SerThr: 2.675 ± 0.048	4.384SerVal: 4.384 ± 0.065	0.485SerTrp: 0.485 ± 0.019	2.541SerTyr: 2.541 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	4.932ThrAla: 4.932 ± 0.081	0.647ThrCys: 0.647 ± 0.02	3.755ThrAsp: 3.755 ± 0.061	3.997ThrGlu: 3.997 ± 0.071	2.164ThrPhe: 2.164 ± 0.041	4.537ThrGly: 4.537 ± 0.063	0.843ThrHis: 0.843 ± 0.022	3.907ThrIle: 3.907 ± 0.071	3.344ThrLys: 3.344 ± 0.056	4.795ThrLeu: 4.795 ± 0.065	1.38ThrMet: 1.38 ± 0.032	2.246ThrAsn: 2.246 ± 0.048	2.007ThrPro: 2.007 ± 0.038	1.931ThrGln: 1.931 ± 0.04	1.86ThrArg: 1.86 ± 0.034	2.797ThrSer: 2.797 ± 0.05	2.731ThrThr: 2.731 ± 0.061	4.884ThrVal: 4.884 ± 0.089	0.495ThrTrp: 0.495 ± 0.022	2.352ThrTyr: 2.352 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	4.382ValAla: 4.382 ± 0.068	1.217ValCys: 1.217 ± 0.032	3.568ValAsp: 3.568 ± 0.054	4.42ValGlu: 4.42 ± 0.062	3.045ValPhe: 3.045 ± 0.051	3.823ValGly: 3.823 ± 0.061	1.032ValHis: 1.032 ± 0.029	5.005ValIle: 5.005 ± 0.063	4.504ValLys: 4.504 ± 0.066	6.267ValLeu: 6.267 ± 0.072	1.914ValMet: 1.914 ± 0.039	3.017ValAsn: 3.017 ± 0.05	2.273ValPro: 2.273 ± 0.043	2.078ValGln: 2.078 ± 0.039	3.093ValArg: 3.093 ± 0.048	4.842ValSer: 4.842 ± 0.064	4.351ValThr: 4.351 ± 0.087	4.432ValVal: 4.432 ± 0.063	0.678ValTrp: 0.678 ± 0.023	2.933ValTyr: 2.933 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.548TrpAla: 0.548 ± 0.02	0.171TrpCys: 0.171 ± 0.011	0.582TrpAsp: 0.582 ± 0.023	0.748TrpGlu: 0.748 ± 0.026	0.413TrpPhe: 0.413 ± 0.019	0.626TrpGly: 0.626 ± 0.023	0.198TrpHis: 0.198 ± 0.013	0.666TrpIle: 0.666 ± 0.023	0.746TrpLys: 0.746 ± 0.025	0.867TrpLeu: 0.867 ± 0.026	0.243TrpMet: 0.243 ± 0.013	0.584TrpAsn: 0.584 ± 0.021	0.13TrpPro: 0.13 ± 0.011	0.39TrpGln: 0.39 ± 0.016	0.421TrpArg: 0.421 ± 0.02	0.492TrpSer: 0.492 ± 0.019	0.447TrpThr: 0.447 ± 0.017	0.534TrpVal: 0.534 ± 0.018	0.105TrpTrp: 0.105 ± 0.009	0.457TrpTyr: 0.457 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.252TyrAla: 3.252 ± 0.05	0.726TyrCys: 0.726 ± 0.026	2.966TyrAsp: 2.966 ± 0.059	3.329TyrGlu: 3.329 ± 0.051	1.911TyrPhe: 1.911 ± 0.037	2.928TyrGly: 2.928 ± 0.045	0.859TyrHis: 0.859 ± 0.03	2.996TyrIle: 2.996 ± 0.044	2.649TyrLys: 2.649 ± 0.046	3.719TyrLeu: 3.719 ± 0.055	1.186TyrMet: 1.186 ± 0.031	2.106TyrAsn: 2.106 ± 0.043	1.374TyrPro: 1.374 ± 0.034	1.626TyrGln: 1.626 ± 0.034	2.201TyrArg: 2.201 ± 0.046	2.439TyrSer: 2.439 ± 0.046	2.54TyrThr: 2.54 ± 0.062	2.698TyrVal: 2.698 ± 0.047	0.423TyrTrp: 0.423 ± 0.02	2.179TyrTyr: 2.179 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4614 proteins (1393863 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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