Turkey aviadenovirus 4
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5NHL0|U5NHL0_9ADEN ORF8 OS=Turkey aviadenovirus 4 OX=1408257 PE=4 SV=1
MM1 pKa = 7.51 SSAPFHH7 pKa = 6.34 GMSISGRR14 pKa = 11.84 KK15 pKa = 9.14 RR16 pKa = 11.84 SSNASNDD23 pKa = 2.91 EE24 pKa = 4.15 AEE26 pKa = 4.42 PVKK29 pKa = 10.72 APKK32 pKa = 9.81 LQAGARR38 pKa = 11.84 VTINAQNAEE47 pKa = 4.35 LDD49 pKa = 3.61 LVYY52 pKa = 10.23 PFWYY56 pKa = 9.92 EE57 pKa = 3.97 STSTGGNPSVNPPLLDD73 pKa = 3.61 PSGPLYY79 pKa = 10.46 VQSNMLKK86 pKa = 9.83 VRR88 pKa = 11.84 TAAPVEE94 pKa = 4.2 VANGSITLAYY104 pKa = 10.41 DD105 pKa = 3.33 DD106 pKa = 5.01 TLTLDD111 pKa = 4.58 DD112 pKa = 4.52 QNKK115 pKa = 9.39 LQINVEE121 pKa = 4.15 PNGPLRR127 pKa = 11.84 STPDD131 pKa = 3.54 GLDD134 pKa = 3.33 LAIDD138 pKa = 3.66 STLEE142 pKa = 3.58 VDD144 pKa = 3.05 NWEE147 pKa = 4.85 LGVKK151 pKa = 10.0 LNPAGPIDD159 pKa = 4.01 ASAAGLDD166 pKa = 3.6 INVDD170 pKa = 3.42 EE171 pKa = 4.61 TLLVAEE177 pKa = 5.32 DD178 pKa = 3.9 STNQRR183 pKa = 11.84 YY184 pKa = 8.55 EE185 pKa = 4.22 LGVHH189 pKa = 6.76 LNPNGPITADD199 pKa = 3.23 SDD201 pKa = 4.26 GLDD204 pKa = 3.85 LEE206 pKa = 5.04 INPEE210 pKa = 4.17 TLTVTNDD217 pKa = 3.11 SNTGGVLSVVLKK229 pKa = 9.94 PQGGLQSSLLGIGVAVDD246 pKa = 3.38 RR247 pKa = 11.84 TLTIDD252 pKa = 3.31 QNTVEE257 pKa = 4.4 VKK259 pKa = 10.08 IDD261 pKa = 3.36 PTGPLTSSDD270 pKa = 3.46 NGLSLDD276 pKa = 3.71 YY277 pKa = 9.8 DD278 pKa = 3.8 TTDD281 pKa = 3.83 FSVQAGKK288 pKa = 10.8 LSLLKK293 pKa = 10.17 TPTVSANAKK302 pKa = 7.85 LTSGSSTMTAFSAYY316 pKa = 8.97 IANSSQQNFNCSYY329 pKa = 11.1 YY330 pKa = 9.65 LQQWLVDD337 pKa = 3.8 GLIMTSLYY345 pKa = 10.8 LKK347 pKa = 10.26 LDD349 pKa = 3.66 RR350 pKa = 11.84 AHH352 pKa = 6.92 FSNMGSDD359 pKa = 3.31 EE360 pKa = 4.2 SSRR363 pKa = 11.84 NAKK366 pKa = 8.79 WFTFWISSTSNFNLSNISEE385 pKa = 4.26 PTIEE389 pKa = 4.46 PSTVQWNAFLPASNYY404 pKa = 8.38 SQPASFQYY412 pKa = 7.27 TTSGGVTNMYY422 pKa = 8.54 YY423 pKa = 10.08 QPSSGAMQTFLPVTTGDD440 pKa = 3.7 WANTTYY446 pKa = 11.25 DD447 pKa = 3.7 PGSVNICALPVSVASSASSSRR468 pKa = 11.84 MMICFNFRR476 pKa = 11.84 CTNSGIFNSNATTGTMSIGPIFFTCPASAYY506 pKa = 7.25 TAPP509 pKa = 4.03
Molecular weight: 54.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.132
IPC2_protein 4.177
IPC_protein 4.177
Toseland 3.961
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.075
Rodwell 3.999
Grimsley 3.872
Solomon 4.151
Lehninger 4.113
Nozaki 4.266
DTASelect 4.507
Thurlkill 3.999
EMBOSS 4.088
Sillero 4.291
Patrickios 3.147
IPC_peptide 4.151
IPC2_peptide 4.279
IPC2.peptide.svr19 4.212
Protein with the highest isoelectric point:
>tr|U5NHK2|U5NHK2_9ADEN DNA-binding protein OS=Turkey aviadenovirus 4 OX=1408257 GN=DBP PE=3 SV=1
MM1 pKa = 7.68 SILISPNDD9 pKa = 3.25 NRR11 pKa = 11.84 GWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SSMRR24 pKa = 11.84 GVGTRR29 pKa = 11.84 RR30 pKa = 11.84 PRR32 pKa = 11.84 RR33 pKa = 11.84 SRR35 pKa = 11.84 MTLRR39 pKa = 11.84 TLLGLGTVSRR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 NRR54 pKa = 11.84 RR55 pKa = 11.84 TRR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 SSRR62 pKa = 11.84 PASTTSRR69 pKa = 11.84 LVVVRR74 pKa = 11.84 TRR76 pKa = 11.84 RR77 pKa = 11.84 GGRR80 pKa = 3.17
Molecular weight: 9.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
32
1
33
12730
67
1268
385.8
43.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.913 ± 0.376
1.948 ± 0.246
5.499 ± 0.224
5.907 ± 0.441
4.108 ± 0.27
5.947 ± 0.332
2.537 ± 0.313
4.478 ± 0.17
4.116 ± 0.377
8.578 ± 0.4
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.632 ± 0.173
4.588 ± 0.319
6.19 ± 0.288
3.778 ± 0.192
6.701 ± 0.458
7.714 ± 0.413
6.237 ± 0.364
6.394 ± 0.318
1.445 ± 0.14
4.289 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here