Chobar Gorge virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4M948|A0A0H4M948_9REOV Core protein VP7 OS=Chobar Gorge virus OX=1679172 GN=VP7 PE=3 SV=1
MM1 pKa = 7.77AEE3 pKa = 3.44QAAAADD9 pKa = 3.7QRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84NPDD16 pKa = 3.0PTAAPYY22 pKa = 10.72LRR24 pKa = 11.84GEE26 pKa = 4.4EE27 pKa = 4.1VRR29 pKa = 11.84DD30 pKa = 3.85DD31 pKa = 4.09NGPLLSVFALQEE43 pKa = 4.01IINKK47 pKa = 8.4VRR49 pKa = 11.84EE50 pKa = 4.16AQTRR54 pKa = 11.84LASSVAEE61 pKa = 4.38TPTASPQVEE70 pKa = 4.06AVLKK74 pKa = 10.71GISDD78 pKa = 4.3LDD80 pKa = 3.85SVKK83 pKa = 10.82GYY85 pKa = 8.6TIQNTVPISYY95 pKa = 9.33IHH97 pKa = 6.63EE98 pKa = 4.24AVQSDD103 pKa = 3.46EE104 pKa = 4.1RR105 pKa = 11.84FFRR108 pKa = 11.84IDD110 pKa = 2.7RR111 pKa = 11.84HH112 pKa = 4.7MLEE115 pKa = 4.4TSAVAADD122 pKa = 4.15VDD124 pKa = 4.14CDD126 pKa = 4.1DD127 pKa = 4.34PQGLITAIVKK137 pKa = 10.12RR138 pKa = 11.84IHH140 pKa = 6.29YY141 pKa = 9.59YY142 pKa = 9.37RR143 pKa = 11.84DD144 pKa = 3.12KK145 pKa = 11.55GSFLLWNTPTHH156 pKa = 6.53FIEE159 pKa = 4.5GRR161 pKa = 11.84EE162 pKa = 4.14VVDD165 pKa = 3.97QAALGVDD172 pKa = 3.02IDD174 pKa = 3.92SLLRR178 pKa = 11.84AVHH181 pKa = 5.44PRR183 pKa = 11.84EE184 pKa = 3.27RR185 pKa = 11.84HH186 pKa = 5.26RR187 pKa = 11.84IQRR190 pKa = 11.84EE191 pKa = 4.1LEE193 pKa = 3.67MHH195 pKa = 6.61LVMNAEE201 pKa = 4.03VRR203 pKa = 11.84GVQVDD208 pKa = 4.21TYY210 pKa = 8.85TTALPQTVYY219 pKa = 10.28EE220 pKa = 4.08VHH222 pKa = 6.34RR223 pKa = 11.84QLTEE227 pKa = 3.85YY228 pKa = 10.73VLEE231 pKa = 4.26GQRR234 pKa = 11.84GVFHH238 pKa = 7.47EE239 pKa = 4.29SMRR242 pKa = 11.84WLQMYY247 pKa = 10.82GEE249 pKa = 4.39FKK251 pKa = 10.77DD252 pKa = 4.94LEE254 pKa = 4.25FSEE257 pKa = 5.33EE258 pKa = 4.45LLTDD262 pKa = 3.65IYY264 pKa = 10.81CTDD267 pKa = 4.16TIYY270 pKa = 11.16CMSYY274 pKa = 10.05HH275 pKa = 6.7LPVNPTVIWEE285 pKa = 4.18VPRR288 pKa = 11.84CGVANLLMNAALGIPTGRR306 pKa = 11.84YY307 pKa = 8.86INPSPKK313 pKa = 9.3IASITVTSRR322 pKa = 11.84VTTTSAFSQMQSIVPTEE339 pKa = 3.61AQMNDD344 pKa = 2.55VRR346 pKa = 11.84KK347 pKa = 9.88IYY349 pKa = 10.11LALMFPNQIVIDD361 pKa = 3.99VRR363 pKa = 11.84AEE365 pKa = 3.78PGNTVDD371 pKa = 4.06QVVHH375 pKa = 6.35AVAGVVGKK383 pKa = 10.69LMFSFGPNIFNITARR398 pKa = 11.84TARR401 pKa = 11.84LLDD404 pKa = 4.07RR405 pKa = 11.84ACSMYY410 pKa = 10.74IRR412 pKa = 11.84MTTDD416 pKa = 2.76DD417 pKa = 3.86RR418 pKa = 11.84RR419 pKa = 11.84TIRR422 pKa = 11.84HH423 pKa = 5.73GRR425 pKa = 11.84TGDD428 pKa = 3.67PLDD431 pKa = 3.63FLIIQGQRR439 pKa = 11.84QVDD442 pKa = 4.17CNQLANDD449 pKa = 3.81PHH451 pKa = 6.38TGAGYY456 pKa = 9.64NAWRR460 pKa = 11.84VDD462 pKa = 3.53DD463 pKa = 5.42LGDD466 pKa = 3.63RR467 pKa = 11.84EE468 pKa = 4.41CPYY471 pKa = 8.84PHH473 pKa = 6.2VRR475 pKa = 11.84RR476 pKa = 11.84FIKK479 pKa = 10.6YY480 pKa = 10.18LGYY483 pKa = 9.08TPEE486 pKa = 6.01DD487 pKa = 3.77IIDD490 pKa = 3.57EE491 pKa = 4.36RR492 pKa = 11.84FTGQDD497 pKa = 2.68HH498 pKa = 7.22RR499 pKa = 11.84FPLMDD504 pKa = 4.34LLGNVLAVAGHH515 pKa = 4.91TNEE518 pKa = 3.97RR519 pKa = 11.84NYY521 pKa = 11.05LEE523 pKa = 5.48GMLQHH528 pKa = 6.06HH529 pKa = 6.56VVRR532 pKa = 11.84FAHH535 pKa = 7.13ISQICNRR542 pKa = 11.84DD543 pKa = 3.25LSSAFSAPDD552 pKa = 3.29DD553 pKa = 3.76RR554 pKa = 11.84FAQLGEE560 pKa = 4.07QIGNGWIAGDD570 pKa = 4.08GPLVLDD576 pKa = 4.11VSYY579 pKa = 10.72HH580 pKa = 6.39AIWFAYY586 pKa = 9.13KK587 pKa = 9.67MRR589 pKa = 11.84FLPISRR595 pKa = 11.84PDD597 pKa = 3.4MQLTQPLIEE606 pKa = 5.01SIYY609 pKa = 10.57GSQISIAKK617 pKa = 9.23YY618 pKa = 7.79QAQRR622 pKa = 11.84IDD624 pKa = 3.48DD625 pKa = 4.24FVRR628 pKa = 11.84EE629 pKa = 4.21NPEE632 pKa = 3.91SFPGIRR638 pKa = 11.84PMDD641 pKa = 3.18VWKK644 pKa = 10.69CVVSEE649 pKa = 3.97LPEE652 pKa = 4.67AIRR655 pKa = 11.84HH656 pKa = 4.47VLEE659 pKa = 5.29LVGQHH664 pKa = 6.12AFINGRR670 pKa = 11.84DD671 pKa = 3.49INLWKK676 pKa = 9.1YY677 pKa = 7.8TPAMQEE683 pKa = 4.23SLPLLCEE690 pKa = 3.71RR691 pKa = 11.84AAWDD695 pKa = 3.49AFRR698 pKa = 11.84GPEE701 pKa = 3.4AVMFTRR707 pKa = 11.84EE708 pKa = 4.07VYY710 pKa = 9.98LHH712 pKa = 6.9RR713 pKa = 11.84EE714 pKa = 4.33IIPEE718 pKa = 3.81FTLEE722 pKa = 3.95DD723 pKa = 3.56VEE725 pKa = 4.51KK726 pKa = 10.46FRR728 pKa = 11.84RR729 pKa = 11.84DD730 pKa = 2.92AEE732 pKa = 4.56YY733 pKa = 9.43YY734 pKa = 10.21TNIHH738 pKa = 6.69LNTPPADD745 pKa = 3.12EE746 pKa = 3.66RR747 pKa = 11.84VYY749 pKa = 11.19LNRR752 pKa = 11.84SSMLQRR758 pKa = 11.84AGEE761 pKa = 4.13GRR763 pKa = 11.84LNIAMRR769 pKa = 11.84RR770 pKa = 11.84YY771 pKa = 10.3LDD773 pKa = 4.17DD774 pKa = 3.77GLYY777 pKa = 9.89VQVGNILRR785 pKa = 11.84PLRR788 pKa = 11.84FQVYY792 pKa = 9.52KK793 pKa = 10.71ALPPLDD799 pKa = 3.52VRR801 pKa = 11.84EE802 pKa = 4.36ALPYY806 pKa = 9.74TYY808 pKa = 10.43TRR810 pKa = 11.84IKK812 pKa = 10.86NDD814 pKa = 2.98GPTARR819 pKa = 11.84VNVSLSKK826 pKa = 10.4SVHH829 pKa = 5.56GFLMLYY835 pKa = 9.11TADD838 pKa = 3.56EE839 pKa = 4.1QAFPDD844 pKa = 3.86EE845 pKa = 4.47MTTLVPAVCLTYY857 pKa = 10.57IKK859 pKa = 9.79TPDD862 pKa = 3.32MPFQRR867 pKa = 11.84VTIDD871 pKa = 3.36DD872 pKa = 3.84ALGVVMRR879 pKa = 11.84DD880 pKa = 2.98VMAVRR885 pKa = 11.84GKK887 pKa = 10.15IRR889 pKa = 11.84IHH891 pKa = 7.26DD892 pKa = 3.64LTAALKK898 pKa = 10.45AGSKK902 pKa = 9.71FASPQLDD909 pKa = 3.03

Molecular weight: 103.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4M5E8|A0A0H4M5E8_9REOV Core protein VP4 OS=Chobar Gorge virus OX=1679172 GN=VP3 PE=3 SV=1
MM1 pKa = 7.51SSEE4 pKa = 4.14RR5 pKa = 11.84VKK7 pKa = 11.13LNSPSGSSMSGLLLGKK23 pKa = 9.79KK24 pKa = 5.91WWKK27 pKa = 10.09EE28 pKa = 3.21IRR30 pKa = 11.84KK31 pKa = 8.97LLRR34 pKa = 11.84ISARR38 pKa = 11.84KK39 pKa = 9.74EE40 pKa = 3.55EE41 pKa = 4.45MLTGEE46 pKa = 4.66YY47 pKa = 10.19DD48 pKa = 3.12RR49 pKa = 11.84KK50 pKa = 8.95TYY52 pKa = 11.22AEE54 pKa = 4.24TLSALRR60 pKa = 11.84TQYY63 pKa = 8.89GTQILKK69 pKa = 10.45RR70 pKa = 11.84EE71 pKa = 4.06TRR73 pKa = 11.84MQMMLNSMPVAQLARR88 pKa = 11.84LPTPTRR94 pKa = 11.84RR95 pKa = 11.84LQRR98 pKa = 11.84TVGTVIVEE106 pKa = 4.12LQRR109 pKa = 11.84TINQTGLEE117 pKa = 4.02RR118 pKa = 11.84WRR120 pKa = 11.84VAVRR124 pKa = 11.84YY125 pKa = 9.28ALTRR129 pKa = 11.84IPRR132 pKa = 11.84AWRR135 pKa = 11.84RR136 pKa = 11.84SEE138 pKa = 4.16RR139 pKa = 11.84IRR141 pKa = 11.84AARR144 pKa = 11.84LFAYY148 pKa = 10.18LRR150 pKa = 11.84AEE152 pKa = 4.22TEE154 pKa = 3.88EE155 pKa = 3.88QRR157 pKa = 11.84QVLATLTRR165 pKa = 11.84LQVEE169 pKa = 4.36EE170 pKa = 4.64RR171 pKa = 11.84LLSTSQISEE180 pKa = 4.46FVTDD184 pKa = 3.35WQEE187 pKa = 3.6KK188 pKa = 8.92LRR190 pKa = 11.84QIFGFFPHH198 pKa = 6.3PQKK201 pKa = 10.94LIRR204 pKa = 11.84QLYY207 pKa = 7.59SSFWVRR213 pKa = 11.84RR214 pKa = 11.84SLSRR218 pKa = 11.84SEE220 pKa = 3.79SRR222 pKa = 11.84RR223 pKa = 11.84IRR225 pKa = 11.84SLNRR229 pKa = 11.84KK230 pKa = 6.64MRR232 pKa = 11.84RR233 pKa = 11.84LGLEE237 pKa = 3.93GG238 pKa = 3.57

Molecular weight: 28.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

6180

192

1284

515.0

57.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.078 ± 0.71

1.343 ± 0.224

5.502 ± 0.439

6.068 ± 0.347

3.058 ± 0.299

5.906 ± 0.225

2.476 ± 0.265

5.55 ± 0.244

3.333 ± 0.426

9.32 ± 0.667

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.816 ± 0.18

2.783 ± 0.298

4.968 ± 0.392

3.932 ± 0.232

8.948 ± 0.459

6.246 ± 0.463

6.634 ± 0.351

7.201 ± 0.323

1.262 ± 0.292

3.576 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski