Cordyceps confragosa (Lecanicillium lecanii)
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8125 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A179IFA8|A0A179IFA8_CORDF DNA polymerase kappa OS=Cordyceps confragosa OX=2714763 GN=LLEC1_02068 PE=4 SV=1
MM1 pKa = 7.31 EE2 pKa = 4.2 QACYY6 pKa = 9.12 PAIIDD11 pKa = 4.33 LLLSYY16 pKa = 10.72 GDD18 pKa = 4.03 KK19 pKa = 10.94 SILGLGDD26 pKa = 4.93 DD27 pKa = 4.26 SGQMALHH34 pKa = 6.48 YY35 pKa = 9.4 AAQMGNTNDD44 pKa = 3.36 IQILTDD50 pKa = 3.34 KK51 pKa = 11.15 GADD54 pKa = 3.46 ADD56 pKa = 5.51 LIDD59 pKa = 3.58 NN60 pKa = 4.91
Molecular weight: 6.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.643
IPC_protein 3.579
Toseland 3.363
ProMoST 3.821
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.643
Rodwell 3.414
Grimsley 3.287
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 4.05
Thurlkill 3.465
EMBOSS 3.643
Sillero 3.719
Patrickios 0.604
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.701
Protein with the highest isoelectric point:
>tr|A0A179I5S8|A0A179I5S8_CORDF Uncharacterized protein OS=Cordyceps confragosa OX=2714763 GN=LLEC1_06952 PE=3 SV=1
MM1 pKa = 7.51 PFSHH5 pKa = 6.98 RR6 pKa = 11.84 KK7 pKa = 7.5 HH8 pKa = 5.89 HH9 pKa = 6.2 HH10 pKa = 4.76 TTVAAPPKK18 pKa = 9.99 RR19 pKa = 11.84 RR20 pKa = 11.84 GLFSRR25 pKa = 11.84 RR26 pKa = 11.84 AAHH29 pKa = 6.24 HH30 pKa = 6.14 HH31 pKa = 5.24 TTTTTTTTAAPRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 GLFSRR50 pKa = 11.84 RR51 pKa = 11.84 PVAQHH56 pKa = 4.68 HH57 pKa = 5.91 HH58 pKa = 4.66 QRR60 pKa = 11.84 KK61 pKa = 8.46 PSLGDD66 pKa = 3.63 KK67 pKa = 10.63 ISGALLKK74 pKa = 10.93 LRR76 pKa = 11.84 GSLTNRR82 pKa = 11.84 PGVKK86 pKa = 9.93 AAGTRR91 pKa = 11.84 RR92 pKa = 11.84 MRR94 pKa = 11.84 GTDD97 pKa = 2.98 GRR99 pKa = 11.84 GSHH102 pKa = 6.11 HH103 pKa = 7.16 HH104 pKa = 5.65 YY105 pKa = 10.98
Molecular weight: 11.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.362
IPC2_protein 10.877
IPC_protein 12.354
Toseland 12.515
ProMoST 13.013
Dawson 12.515
Bjellqvist 12.515
Wikipedia 12.998
Rodwell 12.179
Grimsley 12.559
Solomon 13.013
Lehninger 12.91
Nozaki 12.515
DTASelect 12.515
Thurlkill 12.515
EMBOSS 13.013
Sillero 12.515
Patrickios 11.901
IPC_peptide 13.013
IPC2_peptide 12.003
IPC2.peptide.svr19 9.108
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8125
0
8125
4131730
40
4965
508.5
56.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.405 ± 0.021
1.25 ± 0.011
5.892 ± 0.018
5.909 ± 0.026
3.712 ± 0.014
6.851 ± 0.027
2.407 ± 0.01
4.704 ± 0.016
4.807 ± 0.022
8.893 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.289 ± 0.011
3.566 ± 0.013
5.76 ± 0.026
4.096 ± 0.019
6.137 ± 0.024
8.075 ± 0.031
5.833 ± 0.015
6.167 ± 0.022
1.418 ± 0.009
2.665 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here