Mount Mabu Lophuromys virus 1
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GJ83|A0A2P1GJ83_9MONO Nucleocapsid OS=Mount Mabu Lophuromys virus 1 OX=2116559 GN=N PE=3 SV=1
MM1 pKa = 7.7 AGVDD5 pKa = 3.95 PNLDD9 pKa = 4.27 LINNGIQTIEE19 pKa = 4.68 FIQSNRR25 pKa = 11.84 DD26 pKa = 3.71 EE27 pKa = 4.17 IQKK30 pKa = 9.55 TYY32 pKa = 10.32 GRR34 pKa = 11.84 SSIGQPRR41 pKa = 11.84 TKK43 pKa = 10.11 EE44 pKa = 3.38 RR45 pKa = 11.84 AAAWEE50 pKa = 4.64 AYY52 pKa = 10.29 LEE54 pKa = 4.55 SKK56 pKa = 10.51 DD57 pKa = 3.76 GHH59 pKa = 6.49 PKK61 pKa = 8.24 GQRR64 pKa = 11.84 RR65 pKa = 11.84 GGEE68 pKa = 4.19 SQTSDD73 pKa = 3.44 SEE75 pKa = 4.43 SSSRR79 pKa = 11.84 EE80 pKa = 3.91 GNSDD84 pKa = 3.41 LGSSLSQSSLSEE96 pKa = 3.73 INPRR100 pKa = 11.84 EE101 pKa = 4.09 STGSGNYY108 pKa = 9.47 KK109 pKa = 10.25 GGYY112 pKa = 10.01 DD113 pKa = 3.7 EE114 pKa = 6.34 DD115 pKa = 4.11 PAEE118 pKa = 4.14 LHH120 pKa = 6.53 SGTRR124 pKa = 11.84 DD125 pKa = 3.25 DD126 pKa = 5.93 CRR128 pKa = 11.84 RR129 pKa = 11.84 EE130 pKa = 4.02 SNRR133 pKa = 11.84 NGGGGTIIGEE143 pKa = 4.67 GTDD146 pKa = 3.44 STQGGNSSHH155 pKa = 6.89 EE156 pKa = 4.25 IDD158 pKa = 4.97 DD159 pKa = 3.88 GDD161 pKa = 3.8 YY162 pKa = 11.55 DD163 pKa = 5.21 RR164 pKa = 11.84 ICLLDD169 pKa = 3.64 QATEE173 pKa = 3.72 DD174 pKa = 3.56 HH175 pKa = 6.62 LAGKK179 pKa = 7.43 EE180 pKa = 4.26 TPGKK184 pKa = 10.55 LEE186 pKa = 3.69 VRR188 pKa = 11.84 EE189 pKa = 4.42 ARR191 pKa = 11.84 PSDD194 pKa = 3.04 IAEE197 pKa = 4.26 ILSEE201 pKa = 4.32 DD202 pKa = 3.16 SSRR205 pKa = 11.84 THH207 pKa = 5.86 RR208 pKa = 11.84 RR209 pKa = 11.84 LRR211 pKa = 11.84 GIRR214 pKa = 11.84 SVSRR218 pKa = 11.84 EE219 pKa = 4.16 SEE221 pKa = 4.22 SEE223 pKa = 4.0 EE224 pKa = 4.45 SDD226 pKa = 3.39 VSPVKK231 pKa = 10.55 KK232 pKa = 10.46 GIAGSIVSMSSKK244 pKa = 10.47 GKK246 pKa = 7.92 HH247 pKa = 4.78 TSGSGATQHH256 pKa = 6.78 ALLSPSTPTNRR267 pKa = 11.84 SVSADD272 pKa = 3.54 DD273 pKa = 3.79 ARR275 pKa = 11.84 EE276 pKa = 3.91 SARR279 pKa = 11.84 TARR282 pKa = 11.84 EE283 pKa = 3.26 ISIDD287 pKa = 3.54 DD288 pKa = 3.85 DD289 pKa = 4.46 LGVFSSFGGGSDD301 pKa = 3.18 ISDD304 pKa = 4.03 KK305 pKa = 10.37 IDD307 pKa = 4.13 RR308 pKa = 11.84 MLEE311 pKa = 3.91 NQNEE315 pKa = 4.41 ILSKK319 pKa = 10.44 LQVLAEE325 pKa = 3.93 VSEE328 pKa = 4.43 EE329 pKa = 3.56 IKK331 pKa = 10.81 GIKK334 pKa = 9.62 KK335 pKa = 9.99 ILTNHH340 pKa = 6.04 SLSLSTLEE348 pKa = 5.39 GYY350 pKa = 10.35 INDD353 pKa = 3.97 LMIVIPKK360 pKa = 10.22 SGISNSDD367 pKa = 2.95 GDD369 pKa = 4.44 DD370 pKa = 3.43 EE371 pKa = 5.19 KK372 pKa = 11.67 NPDD375 pKa = 3.14 LRR377 pKa = 11.84 MVVGRR382 pKa = 11.84 DD383 pKa = 2.97 KK384 pKa = 11.4 SRR386 pKa = 11.84 GVTEE390 pKa = 3.59 VSKK393 pKa = 10.82 RR394 pKa = 11.84 KK395 pKa = 9.54 RR396 pKa = 11.84 PKK398 pKa = 8.69 STFGEE403 pKa = 4.41 EE404 pKa = 3.47 IGTGRR409 pKa = 11.84 TIDD412 pKa = 3.7 EE413 pKa = 4.89 EE414 pKa = 4.51 YY415 pKa = 11.26 LLDD418 pKa = 4.64 PLDD421 pKa = 4.02 FKK423 pKa = 11.6 KK424 pKa = 10.81 NNAANFVPDD433 pKa = 3.71 NTPVSMSIIASMVKK447 pKa = 10.11 KK448 pKa = 10.16 RR449 pKa = 11.84 VHH451 pKa = 7.11 DD452 pKa = 4.27 PDD454 pKa = 3.74 TRR456 pKa = 11.84 DD457 pKa = 3.34 EE458 pKa = 4.52 LLMLVEE464 pKa = 5.02 SNLGSIPMKK473 pKa = 10.77 DD474 pKa = 3.02 MYY476 pKa = 10.85 EE477 pKa = 4.13 QIRR480 pKa = 11.84 KK481 pKa = 9.5 HH482 pKa = 5.96 IDD484 pKa = 3.13 QMDD487 pKa = 3.37 SS488 pKa = 2.94
Molecular weight: 53.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.0
IPC2_protein 4.927
IPC_protein 4.889
Toseland 4.774
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.724
Rodwell 4.749
Grimsley 4.685
Solomon 4.851
Lehninger 4.813
Nozaki 4.965
DTASelect 5.13
Thurlkill 4.762
EMBOSS 4.749
Sillero 5.029
Patrickios 4.418
IPC_peptide 4.863
IPC2_peptide 5.016
IPC2.peptide.svr19 5.025
Protein with the highest isoelectric point:
>tr|A0A2P1GJA5|A0A2P1GJA5_9MONO Matrix protein OS=Mount Mabu Lophuromys virus 1 OX=2116559 GN=M PE=4 SV=1
MM1 pKa = 7.46 YY2 pKa = 10.18 YY3 pKa = 10.28 KK4 pKa = 10.69 KK5 pKa = 10.7 LRR7 pKa = 11.84 IKK9 pKa = 10.18 VIHH12 pKa = 6.11 ITMEE16 pKa = 4.1 GKK18 pKa = 10.45 ADD20 pKa = 4.19 FLPSSWSEE28 pKa = 3.59 GGILHH33 pKa = 7.39 AIEE36 pKa = 5.77 AEE38 pKa = 3.95 ADD40 pKa = 3.79 DD41 pKa = 4.46 EE42 pKa = 4.8 GKK44 pKa = 10.45 LVPKK48 pKa = 10.29 YY49 pKa = 10.37 RR50 pKa = 11.84 VVNPGWNSRR59 pKa = 11.84 KK60 pKa = 10.14 GSGFMYY66 pKa = 10.6 LLIYY70 pKa = 9.71 GIIEE74 pKa = 4.06 EE75 pKa = 4.95 KK76 pKa = 10.34 EE77 pKa = 4.24 SKK79 pKa = 10.24 DD80 pKa = 3.21 KK81 pKa = 11.12 KK82 pKa = 10.54 NRR84 pKa = 11.84 GFKK87 pKa = 10.73 SFGAFPLGVGRR98 pKa = 11.84 SRR100 pKa = 11.84 ADD102 pKa = 3.38 PQDD105 pKa = 3.92 LLDD108 pKa = 5.57 AVLQLDD114 pKa = 3.34 ITVRR118 pKa = 11.84 RR119 pKa = 11.84 TAGAGEE125 pKa = 4.27 KK126 pKa = 10.54 LVMGTNNINPILTPWKK142 pKa = 10.52 GILTNGAVFQAIKK155 pKa = 10.33 VCNNVDD161 pKa = 3.72 TIMVDD166 pKa = 3.4 RR167 pKa = 11.84 PQKK170 pKa = 10.24 LRR172 pKa = 11.84 PIFLTITLLTDD183 pKa = 3.93 AGCYY187 pKa = 9.55 KK188 pKa = 10.35 IPRR191 pKa = 11.84 TILDD195 pKa = 3.74 FRR197 pKa = 11.84 ASGAMSFNLLVVLSIGADD215 pKa = 3.44 FTPYY219 pKa = 8.34 GTKK222 pKa = 10.78 GMINEE227 pKa = 4.52 DD228 pKa = 3.87 GEE230 pKa = 4.55 RR231 pKa = 11.84 VTTFMVHH238 pKa = 5.85 IGNFVRR244 pKa = 11.84 RR245 pKa = 11.84 CGKK248 pKa = 9.89 EE249 pKa = 3.61 YY250 pKa = 10.9 SVDD253 pKa = 3.38 YY254 pKa = 10.77 CRR256 pKa = 11.84 QKK258 pKa = 10.21 VDD260 pKa = 3.46 KK261 pKa = 9.78 MDD263 pKa = 3.58 LRR265 pKa = 11.84 FSLGAVGGLSFHH277 pKa = 6.77 VKK279 pKa = 10.21 LAGKK283 pKa = 9.68 VSKK286 pKa = 8.5 TLRR289 pKa = 11.84 AQLGHH294 pKa = 6.65 RR295 pKa = 11.84 TTICYY300 pKa = 10.8 SLMDD304 pKa = 4.0 TNPYY308 pKa = 9.65 LNRR311 pKa = 11.84 VMWKK315 pKa = 10.16 AEE317 pKa = 3.75 CSIQKK322 pKa = 8.05 VTAVFQPSVPRR333 pKa = 11.84 DD334 pKa = 3.23 FKK336 pKa = 11.1 IYY338 pKa = 10.6 DD339 pKa = 3.84 DD340 pKa = 4.07 VLIDD344 pKa = 3.55 HH345 pKa = 7.21 TGKK348 pKa = 10.1 ILKK351 pKa = 9.27 QQ352 pKa = 3.26
Molecular weight: 39.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.878
IPC2_protein 8.814
IPC_protein 8.712
Toseland 9.487
ProMoST 9.238
Dawson 9.75
Bjellqvist 9.472
Wikipedia 9.911
Rodwell 10.101
Grimsley 9.809
Solomon 9.78
Lehninger 9.75
Nozaki 9.633
DTASelect 9.428
Thurlkill 9.604
EMBOSS 9.926
Sillero 9.706
Patrickios 5.245
IPC_peptide 9.78
IPC2_peptide 8.097
IPC2.peptide.svr19 7.91
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5363
155
2174
670.4
75.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.96 ± 0.639
1.417 ± 0.273
6.023 ± 0.36
5.519 ± 0.714
3.636 ± 0.512
5.948 ± 0.806
1.846 ± 0.396
7.869 ± 0.451
6.172 ± 0.373
9.528 ± 0.745
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.666 ± 0.238
5.762 ± 0.545
4.55 ± 0.439
3.841 ± 0.233
5.221 ± 0.51
8.186 ± 0.73
6.228 ± 0.771
5.668 ± 0.36
0.951 ± 0.178
4.009 ± 0.671
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here