Striga asiatica (Asiatic witchweed) (Buchnera asiatica)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Orobanchaceae; Buchnereae; Striga

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33322 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A7PX02|A0A5A7PX02_STRAF ABC-type multidrug transport system OS=Striga asiatica OX=4170 GN=STAS_13778 PE=4 SV=1
MM1 pKa = 7.08QGVVLDD7 pKa = 4.43RR8 pKa = 11.84SMLDD12 pKa = 3.37SLYY15 pKa = 10.98DD16 pKa = 3.66AAMTQNNTNGTSLQMAGVPSNNPFEE41 pKa = 5.62SDD43 pKa = 3.8DD44 pKa = 3.91YY45 pKa = 9.29STQMFYY51 pKa = 11.18DD52 pKa = 4.47PSQPNQLQLAEE63 pKa = 4.89IPQQQQTQQMIGYY76 pKa = 9.18DD77 pKa = 3.78YY78 pKa = 10.31KK79 pKa = 11.03QVIGIPQQQQEE90 pKa = 4.4TSLVVYY96 pKa = 10.18DD97 pKa = 4.74SNQQQEE103 pKa = 3.94QSFVNKK109 pKa = 10.34NLMQMTDD116 pKa = 3.55TPLEE120 pKa = 4.13TPMQQQEE127 pKa = 4.15KK128 pKa = 9.34PIVAFEE134 pKa = 3.93PMGNSRR140 pKa = 11.84NPFDD144 pKa = 3.58

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A7NZI8|A0A5A7NZI8_STRAF NAD(P)-binding Rossmann-fold superfamily protein OS=Striga asiatica OX=4170 GN=STAS_01571 PE=4 SV=1
MM1 pKa = 7.97KK2 pKa = 10.05IRR4 pKa = 11.84ASVRR8 pKa = 11.84KK9 pKa = 8.91ICKK12 pKa = 8.34KK13 pKa = 9.8CRR15 pKa = 11.84LIRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84GRR22 pKa = 11.84IINMTKK28 pKa = 9.96ARR30 pKa = 11.84PRR32 pKa = 11.84TGSRR36 pKa = 11.84RR37 pKa = 11.84NVRR40 pKa = 11.84IGSRR44 pKa = 11.84RR45 pKa = 11.84IVRR48 pKa = 11.84IGSRR52 pKa = 11.84RR53 pKa = 11.84KK54 pKa = 9.7VRR56 pKa = 11.84IISRR60 pKa = 11.84KK61 pKa = 6.11STHH64 pKa = 7.22RR65 pKa = 11.84IPKK68 pKa = 9.97GLIHH72 pKa = 5.96VQSSFNNTIVTVTDD86 pKa = 3.22LRR88 pKa = 11.84GRR90 pKa = 11.84VLSWSSAGTLGFKK103 pKa = 10.4GPRR106 pKa = 11.84KK107 pKa = 8.48RR108 pKa = 11.84TPFAAQTTAADD119 pKa = 4.99AIRR122 pKa = 11.84TVVDD126 pKa = 2.88QGMQRR131 pKa = 11.84AAVMLKK137 pKa = 10.43GAA139 pKa = 4.54

Molecular weight: 15.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33322

0

33322

12314773

99

5358

369.6

41.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.916 ± 0.013

1.897 ± 0.006

5.242 ± 0.009

6.406 ± 0.013

4.191 ± 0.009

6.737 ± 0.013

2.421 ± 0.006

5.108 ± 0.009

5.916 ± 0.012

9.577 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.005

4.363 ± 0.009

5.24 ± 0.013

3.422 ± 0.01

6.012 ± 0.011

8.831 ± 0.013

4.793 ± 0.008

6.606 ± 0.01

1.325 ± 0.005

2.676 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski