Salmonella phage vB_StyS-sam

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5K7YBR7|A0A5K7YBR7_9CAUD Uncharacterized protein OS=Salmonella phage vB_StyS-sam OX=2664131 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.45SKK4 pKa = 10.9VLIVTIEE11 pKa = 4.18TAWVGASEE19 pKa = 4.31VVEE22 pKa = 4.17IEE24 pKa = 3.98LDD26 pKa = 3.51GSEE29 pKa = 4.27TEE31 pKa = 4.8EE32 pKa = 4.44EE33 pKa = 4.41LEE35 pKa = 4.78DD36 pKa = 3.55MAKK39 pKa = 10.58EE40 pKa = 4.02EE41 pKa = 4.14FGNYY45 pKa = 8.92CNYY48 pKa = 8.92GWRR51 pKa = 11.84VEE53 pKa = 4.24EE54 pKa = 4.19

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5K7YCA0|A0A5K7YCA0_9CAUD Uncharacterized protein OS=Salmonella phage vB_StyS-sam OX=2664131 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84EE3 pKa = 4.1LPRR6 pKa = 11.84PSSTKK11 pKa = 10.2LATLILEE18 pKa = 4.88GSTKK22 pKa = 10.19PVIPLTVEE30 pKa = 4.28LGASIVMLCSVVEE43 pKa = 3.93RR44 pKa = 11.84MPTKK48 pKa = 10.15RR49 pKa = 11.84RR50 pKa = 11.84PFTRR54 pKa = 11.84QAPEE58 pKa = 3.43IYY60 pKa = 9.43IWFAGKK66 pKa = 9.9FLVLVSIIAILVNTVSWARR85 pKa = 11.84II86 pKa = 3.5

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13309

33

828

201.7

22.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.467 ± 0.59

1.187 ± 0.158

5.801 ± 0.213

6.544 ± 0.415

3.689 ± 0.181

7.521 ± 0.348

1.713 ± 0.182

5.034 ± 0.23

5.793 ± 0.325

7.754 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.148

4.501 ± 0.228

4.11 ± 0.27

3.509 ± 0.232

6.034 ± 0.28

6.499 ± 0.327

6.439 ± 0.313

7.356 ± 0.291

1.352 ± 0.132

3.186 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski