Pseudonocardia sp. HH130629-09

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; unclassified Pseudonocardia

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4780 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3UB28|A0A0M3UB28_9PSEU Luciferase OS=Pseudonocardia sp. HH130629-09 OX=1641402 GN=XF36_10420 PE=4 SV=1
MM1 pKa = 7.79ALQLDD6 pKa = 4.12PQLLEE11 pKa = 5.06ILACPCDD18 pKa = 3.54AHH20 pKa = 7.45AVLTPGEE27 pKa = 4.24DD28 pKa = 3.11RR29 pKa = 11.84LTCTACGRR37 pKa = 11.84AFPVTDD43 pKa = 5.51GIPVLLLDD51 pKa = 4.18EE52 pKa = 5.17ALPGTGPAGDD62 pKa = 4.2GRR64 pKa = 11.84DD65 pKa = 3.7DD66 pKa = 3.67GG67 pKa = 4.63

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4QED5|A0A0M4QED5_9PSEU Uncharacterized protein OS=Pseudonocardia sp. HH130629-09 OX=1641402 GN=XF36_25585 PE=4 SV=1
MM1 pKa = 7.58SSTATSKK8 pKa = 11.18APASSPWPKK17 pKa = 10.83SPMPWTRR24 pKa = 11.84NVKK27 pKa = 10.48FSRR30 pKa = 11.84PPSAASRR37 pKa = 11.84VASAARR43 pKa = 11.84APFGSMPTTSRR54 pKa = 11.84HH55 pKa = 5.34RR56 pKa = 11.84PASSSAVV63 pKa = 3.29

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4780

0

4780

1499939

30

9453

313.8

33.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.153 ± 0.063

0.744 ± 0.01

6.535 ± 0.03

5.297 ± 0.037

2.557 ± 0.02

9.873 ± 0.036

2.256 ± 0.018

2.959 ± 0.028

1.375 ± 0.026

10.353 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.63 ± 0.012

1.472 ± 0.018

6.322 ± 0.04

2.354 ± 0.018

8.521 ± 0.046

4.684 ± 0.025

6.178 ± 0.026

9.578 ± 0.035

1.449 ± 0.013

1.711 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski