Amino acid dipepetide frequency for Pseudonocardia sp. HH130629-09

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.433AlaAla: 23.433 ± 0.222	1.021AlaCys: 1.021 ± 0.028	9.301AlaAsp: 9.301 ± 0.092	8.587AlaGlu: 8.587 ± 0.095	3.403AlaPhe: 3.403 ± 0.058	15.356AlaGly: 15.356 ± 0.137	2.885AlaHis: 2.885 ± 0.049	3.553AlaIle: 3.553 ± 0.059	1.797AlaLys: 1.797 ± 0.044	14.81AlaLeu: 14.81 ± 0.127	2.463AlaMet: 2.463 ± 0.04	1.667AlaAsn: 1.667 ± 0.037	7.604AlaPro: 7.604 ± 0.102	3.319AlaGln: 3.319 ± 0.052	11.426AlaArg: 11.426 ± 0.103	5.39AlaSer: 5.39 ± 0.059	7.806AlaThr: 7.806 ± 0.092	13.909AlaVal: 13.909 ± 0.108	1.812AlaTrp: 1.812 ± 0.042	1.986AlaTyr: 1.986 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.094CysAla: 1.094 ± 0.031	0.083CysCys: 0.083 ± 0.009	0.49CysAsp: 0.49 ± 0.017	0.369CysGlu: 0.369 ± 0.017	0.185CysPhe: 0.185 ± 0.011	0.984CysGly: 0.984 ± 0.027	0.158CysHis: 0.158 ± 0.012	0.185CysIle: 0.185 ± 0.012	0.092CysLys: 0.092 ± 0.008	0.601CysLeu: 0.601 ± 0.021	0.094CysMet: 0.094 ± 0.008	0.103CysAsn: 0.103 ± 0.008	0.487CysPro: 0.487 ± 0.02	0.123CysGln: 0.123 ± 0.009	0.628CysArg: 0.628 ± 0.024	0.423CysSer: 0.423 ± 0.019	0.45CysThr: 0.45 ± 0.017	0.617CysVal: 0.617 ± 0.022	0.12CysTrp: 0.12 ± 0.009	0.152CysTyr: 0.152 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	9.069AspAla: 9.069 ± 0.095	0.377AspCys: 0.377 ± 0.017	4.716AspAsp: 4.716 ± 0.066	4.015AspGlu: 4.015 ± 0.059	1.265AspPhe: 1.265 ± 0.032	7.308AspGly: 7.308 ± 0.075	1.529AspHis: 1.529 ± 0.033	1.483AspIle: 1.483 ± 0.037	0.729AspLys: 0.729 ± 0.025	6.832AspLeu: 6.832 ± 0.068	0.775AspMet: 0.775 ± 0.023	0.711AspAsn: 0.711 ± 0.022	5.69AspPro: 5.69 ± 0.07	1.361AspGln: 1.361 ± 0.035	6.163AspArg: 6.163 ± 0.072	2.071AspSer: 2.071 ± 0.045	3.513AspThr: 3.513 ± 0.049	5.801AspVal: 5.801 ± 0.067	0.897AspTrp: 0.897 ± 0.026	1.047AspTyr: 1.047 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	6.077GluAla: 6.077 ± 0.079	0.296GluCys: 0.296 ± 0.016	2.337GluAsp: 2.337 ± 0.045	2.495GluGlu: 2.495 ± 0.05	1.537GluPhe: 1.537 ± 0.031	3.541GluGly: 3.541 ± 0.052	1.662GluHis: 1.662 ± 0.036	2.405GluIle: 2.405 ± 0.042	1.161GluLys: 1.161 ± 0.038	6.99GluLeu: 6.99 ± 0.086	0.83GluMet: 0.83 ± 0.023	0.935GluAsn: 0.935 ± 0.027	3.37GluPro: 3.37 ± 0.054	2.336GluGln: 2.336 ± 0.047	5.518GluArg: 5.518 ± 0.07	2.229GluSer: 2.229 ± 0.04	2.75GluThr: 2.75 ± 0.044	4.912GluVal: 4.912 ± 0.06	0.711GluTrp: 0.711 ± 0.022	0.88GluTyr: 0.88 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.742PheAla: 3.742 ± 0.058	0.265PheCys: 0.265 ± 0.013	2.029PheAsp: 2.029 ± 0.042	1.165PheGlu: 1.165 ± 0.032	0.765PhePhe: 0.765 ± 0.029	3.017PheGly: 3.017 ± 0.048	0.603PheHis: 0.603 ± 0.021	0.511PheIle: 0.511 ± 0.019	0.297PheLys: 0.297 ± 0.014	2.433PheLeu: 2.433 ± 0.051	0.289PheMet: 0.289 ± 0.014	0.391PheAsn: 0.391 ± 0.019	1.323PhePro: 1.323 ± 0.03	0.467PheGln: 0.467 ± 0.015	1.774PheArg: 1.774 ± 0.037	1.343PheSer: 1.343 ± 0.034	1.807PheThr: 1.807 ± 0.041	2.453PheVal: 2.453 ± 0.044	0.394PheTrp: 0.394 ± 0.019	0.499PheTyr: 0.499 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	12.174GlyAla: 12.174 ± 0.124	0.847GlyCys: 0.847 ± 0.028	5.655GlyAsp: 5.655 ± 0.058	5.122GlyGlu: 5.122 ± 0.058	2.922GlyPhe: 2.922 ± 0.051	9.167GlyGly: 9.167 ± 0.099	2.337GlyHis: 2.337 ± 0.046	3.476GlyIle: 3.476 ± 0.059	1.569GlyLys: 1.569 ± 0.044	9.885GlyLeu: 9.885 ± 0.087	2.078GlyMet: 2.078 ± 0.036	1.475GlyAsn: 1.475 ± 0.031	6.258GlyPro: 6.258 ± 0.08	2.34GlyGln: 2.34 ± 0.051	8.574GlyArg: 8.574 ± 0.086	5.286GlySer: 5.286 ± 0.06	6.771GlyThr: 6.771 ± 0.084	8.866GlyVal: 8.866 ± 0.072	1.814GlyTrp: 1.814 ± 0.039	2.111GlyTyr: 2.111 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.713HisAla: 2.713 ± 0.043	0.189HisCys: 0.189 ± 0.011	1.655HisAsp: 1.655 ± 0.034	1.137HisGlu: 1.137 ± 0.032	0.519HisPhe: 0.519 ± 0.021	2.558HisGly: 2.558 ± 0.04	0.715HisHis: 0.715 ± 0.03	0.462HisIle: 0.462 ± 0.018	0.227HisLys: 0.227 ± 0.012	2.449HisLeu: 2.449 ± 0.041	0.274HisMet: 0.274 ± 0.015	0.289HisAsn: 0.289 ± 0.013	1.824HisPro: 1.824 ± 0.037	0.491HisGln: 0.491 ± 0.021	2.222HisArg: 2.222 ± 0.042	0.927HisSer: 0.927 ± 0.03	1.238HisThr: 1.238 ± 0.03	1.903HisVal: 1.903 ± 0.035	0.323HisTrp: 0.323 ± 0.015	0.444HisTyr: 0.444 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	4.735IleAla: 4.735 ± 0.07	0.259IleCys: 0.259 ± 0.013	2.254IleAsp: 2.254 ± 0.042	1.802IleGlu: 1.802 ± 0.036	0.579IlePhe: 0.579 ± 0.022	3.605IleGly: 3.605 ± 0.059	0.5IleHis: 0.5 ± 0.017	0.71IleIle: 0.71 ± 0.03	0.442IleLys: 0.442 ± 0.018	2.134IleLeu: 2.134 ± 0.042	0.405IleMet: 0.405 ± 0.018	0.601IleAsn: 0.601 ± 0.024	1.619IlePro: 1.619 ± 0.038	0.515IleGln: 0.515 ± 0.021	2.129IleArg: 2.129 ± 0.04	1.491IleSer: 1.491 ± 0.03	2.019IleThr: 2.019 ± 0.038	2.985IleVal: 2.985 ± 0.051	0.345IleTrp: 0.345 ± 0.015	0.461IleTyr: 0.461 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	1.743LysAla: 1.743 ± 0.047	0.077LysCys: 0.077 ± 0.007	0.737LysAsp: 0.737 ± 0.024	0.66LysGlu: 0.66 ± 0.027	0.314LysPhe: 0.314 ± 0.015	1.107LysGly: 1.107 ± 0.035	0.304LysHis: 0.304 ± 0.017	0.689LysIle: 0.689 ± 0.025	0.444LysLys: 0.444 ± 0.02	1.446LysLeu: 1.446 ± 0.035	0.257LysMet: 0.257 ± 0.016	0.311LysAsn: 0.311 ± 0.017	0.819LysPro: 0.819 ± 0.028	0.447LysGln: 0.447 ± 0.019	1.083LysArg: 1.083 ± 0.033	0.693LysSer: 0.693 ± 0.024	0.821LysThr: 0.821 ± 0.026	1.359LysVal: 1.359 ± 0.038	0.165LysTrp: 0.165 ± 0.011	0.268LysTyr: 0.268 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	16.14LeuAla: 16.14 ± 0.133	0.76LeuCys: 0.76 ± 0.02	7.762LeuAsp: 7.762 ± 0.078	4.411LeuGlu: 4.411 ± 0.067	2.507LeuPhe: 2.507 ± 0.041	9.746LeuGly: 9.746 ± 0.091	2.389LeuHis: 2.389 ± 0.045	2.602LeuIle: 2.602 ± 0.049	1.144LeuLys: 1.144 ± 0.032	11.27LeuLeu: 11.27 ± 0.116	1.339LeuMet: 1.339 ± 0.031	1.362LeuAsn: 1.362 ± 0.033	6.422LeuPro: 6.422 ± 0.075	2.041LeuGln: 2.041 ± 0.037	9.074LeuArg: 9.074 ± 0.101	5.01LeuSer: 5.01 ± 0.066	6.392LeuThr: 6.392 ± 0.059	10.443LeuVal: 10.443 ± 0.092	1.187LeuTrp: 1.187 ± 0.031	1.532LeuTyr: 1.532 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	2.013MetAla: 2.013 ± 0.035	0.123MetCys: 0.123 ± 0.009	0.775MetAsp: 0.775 ± 0.021	0.608MetGlu: 0.608 ± 0.02	0.454MetPhe: 0.454 ± 0.017	1.243MetGly: 1.243 ± 0.031	0.343MetHis: 0.343 ± 0.016	0.685MetIle: 0.685 ± 0.022	0.28MetLys: 0.28 ± 0.016	1.776MetLeu: 1.776 ± 0.031	0.221MetMet: 0.221 ± 0.013	0.34MetAsn: 0.34 ± 0.015	1.043MetPro: 1.043 ± 0.027	0.4MetGln: 0.4 ± 0.016	1.367MetArg: 1.367 ± 0.03	1.257MetSer: 1.257 ± 0.029	1.54MetThr: 1.54 ± 0.032	1.405MetVal: 1.405 ± 0.033	0.189MetTrp: 0.189 ± 0.012	0.233MetTyr: 0.233 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	1.901AsnAla: 1.901 ± 0.037	0.115AsnCys: 0.115 ± 0.009	0.861AsnAsp: 0.861 ± 0.028	0.613AsnGlu: 0.613 ± 0.019	0.39AsnPhe: 0.39 ± 0.018	1.567AsnGly: 1.567 ± 0.039	0.33AsnHis: 0.33 ± 0.015	0.512AsnIle: 0.512 ± 0.02	0.239AsnLys: 0.239 ± 0.014	1.513AsnLeu: 1.513 ± 0.035	0.243AsnMet: 0.243 ± 0.013	0.319AsnAsn: 0.319 ± 0.017	1.149AsnPro: 1.149 ± 0.031	0.409AsnGln: 0.409 ± 0.019	1.188AsnArg: 1.188 ± 0.031	0.67AsnSer: 0.67 ± 0.023	0.912AsnThr: 0.912 ± 0.027	1.245AsnVal: 1.245 ± 0.032	0.217AsnTrp: 0.217 ± 0.011	0.327AsnTyr: 0.327 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.438ProAla: 9.438 ± 0.105	0.345ProCys: 0.345 ± 0.017	5.427ProAsp: 5.427 ± 0.068	4.077ProGlu: 4.077 ± 0.057	1.467ProPhe: 1.467 ± 0.032	7.456ProGly: 7.456 ± 0.089	1.328ProHis: 1.328 ± 0.033	1.431ProIle: 1.431 ± 0.033	0.794ProLys: 0.794 ± 0.027	5.172ProLeu: 5.172 ± 0.065	1.021ProMet: 1.021 ± 0.029	0.832ProAsn: 0.832 ± 0.022	3.581ProPro: 3.581 ± 0.078	1.621ProGln: 1.621 ± 0.033	4.284ProArg: 4.284 ± 0.058	3.122ProSer: 3.122 ± 0.047	3.553ProThr: 3.553 ± 0.059	6.312ProVal: 6.312 ± 0.068	0.924ProTrp: 0.924 ± 0.025	1.029ProTyr: 1.029 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.085GlnAla: 3.085 ± 0.049	0.148GlnCys: 0.148 ± 0.011	1.176GlnAsp: 1.176 ± 0.027	1.038GlnGlu: 1.038 ± 0.029	0.594GlnPhe: 0.594 ± 0.022	1.861GlnGly: 1.861 ± 0.044	0.561GlnHis: 0.561 ± 0.018	0.989GlnIle: 0.989 ± 0.025	0.42GlnLys: 0.42 ± 0.021	2.75GlnLeu: 2.75 ± 0.041	0.438GlnMet: 0.438 ± 0.015	0.453GlnAsn: 0.453 ± 0.02	1.282GlnPro: 1.282 ± 0.029	0.985GlnGln: 0.985 ± 0.036	2.331GlnArg: 2.331 ± 0.043	0.966GlnSer: 0.966 ± 0.028	1.154GlnThr: 1.154 ± 0.031	2.47GlnVal: 2.47 ± 0.038	0.353GlnTrp: 0.353 ± 0.016	0.483GlnTyr: 0.483 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	11.172ArgAla: 11.172 ± 0.111	0.653ArgCys: 0.653 ± 0.023	5.074ArgAsp: 5.074 ± 0.064	4.75ArgGlu: 4.75 ± 0.066	2.481ArgPhe: 2.481 ± 0.049	6.455ArgGly: 6.455 ± 0.07	2.072ArgHis: 2.072 ± 0.035	3.41ArgIle: 3.41 ± 0.046	1.087ArgLys: 1.087 ± 0.033	8.65ArgLeu: 8.65 ± 0.087	1.873ArgMet: 1.873 ± 0.033	1.299ArgAsn: 1.299 ± 0.039	5.46ArgPro: 5.46 ± 0.069	1.775ArgGln: 1.775 ± 0.036	8.687ArgArg: 8.687 ± 0.107	4.436ArgSer: 4.436 ± 0.059	5.644ArgThr: 5.644 ± 0.06	6.931ArgVal: 6.931 ± 0.074	1.532ArgTrp: 1.532 ± 0.032	1.774ArgTyr: 1.774 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	6.194SerAla: 6.194 ± 0.065	0.381SerCys: 0.381 ± 0.016	2.7SerAsp: 2.7 ± 0.04	2.208SerGlu: 2.208 ± 0.036	1.345SerPhe: 1.345 ± 0.03	5.734SerGly: 5.734 ± 0.069	0.81SerHis: 0.81 ± 0.023	1.339SerIle: 1.339 ± 0.031	0.64SerLys: 0.64 ± 0.021	4.272SerLeu: 4.272 ± 0.057	1.052SerMet: 1.052 ± 0.026	0.712SerAsn: 0.712 ± 0.024	3.115SerPro: 3.115 ± 0.053	1.021SerGln: 1.021 ± 0.029	3.661SerArg: 3.661 ± 0.047	2.633SerSer: 2.633 ± 0.051	3.072SerThr: 3.072 ± 0.046	4.186SerVal: 4.186 ± 0.053	0.869SerTrp: 0.869 ± 0.021	0.894SerTyr: 0.894 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.05ThrAla: 9.05 ± 0.091	0.459ThrCys: 0.459 ± 0.017	4.028ThrAsp: 4.028 ± 0.06	3.251ThrGlu: 3.251 ± 0.05	1.517ThrPhe: 1.517 ± 0.03	7.216ThrGly: 7.216 ± 0.084	1.168ThrHis: 1.168 ± 0.029	1.752ThrIle: 1.752 ± 0.039	0.754ThrLys: 0.754 ± 0.026	5.571ThrLeu: 5.571 ± 0.071	0.927ThrMet: 0.927 ± 0.026	0.861ThrAsn: 0.861 ± 0.026	4.224ThrPro: 4.224 ± 0.058	1.046ThrGln: 1.046 ± 0.026	4.394ThrArg: 4.394 ± 0.061	2.809ThrSer: 2.809 ± 0.047	4.132ThrThr: 4.132 ± 0.061	6.714ThrVal: 6.714 ± 0.061	0.891ThrTrp: 0.891 ± 0.026	1.016ThrTyr: 1.016 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	13.814ValAla: 13.814 ± 0.109	0.739ValCys: 0.739 ± 0.024	6.379ValAsp: 6.379 ± 0.069	5.182ValGlu: 5.182 ± 0.059	2.318ValPhe: 2.318 ± 0.044	7.955ValGly: 7.955 ± 0.094	2.17ValHis: 2.17 ± 0.041	2.545ValIle: 2.545 ± 0.052	1.117ValLys: 1.117 ± 0.033	11.387ValLeu: 11.387 ± 0.108	1.266ValMet: 1.266 ± 0.034	1.428ValAsn: 1.428 ± 0.031	6.117ValPro: 6.117 ± 0.074	1.981ValGln: 1.981 ± 0.039	7.684ValArg: 7.684 ± 0.083	4.316ValSer: 4.316 ± 0.058	6.179ValThr: 6.179 ± 0.072	10.724ValVal: 10.724 ± 0.108	1.093ValTrp: 1.093 ± 0.032	1.383ValTyr: 1.383 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.731TrpAla: 1.731 ± 0.038	0.143TrpCys: 0.143 ± 0.009	0.807TrpAsp: 0.807 ± 0.023	0.649TrpGlu: 0.649 ± 0.024	0.519TrpPhe: 0.519 ± 0.02	1.041TrpGly: 1.041 ± 0.03	0.323TrpHis: 0.323 ± 0.015	0.508TrpIle: 0.508 ± 0.019	0.251TrpLys: 0.251 ± 0.013	1.626TrpLeu: 1.626 ± 0.037	0.275TrpMet: 0.275 ± 0.015	0.31TrpAsn: 0.31 ± 0.013	0.824TrpPro: 0.824 ± 0.024	0.439TrpGln: 0.439 ± 0.017	1.321TrpArg: 1.321 ± 0.03	0.949TrpSer: 0.949 ± 0.024	1.044TrpThr: 1.044 ± 0.028	1.115TrpVal: 1.115 ± 0.03	0.359TrpTrp: 0.359 ± 0.016	0.253TrpTyr: 0.253 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.211TyrAla: 2.211 ± 0.037	0.159TyrCys: 0.159 ± 0.011	1.187TyrAsp: 1.187 ± 0.028	0.835TyrGlu: 0.835 ± 0.026	0.487TyrPhe: 0.487 ± 0.019	1.808TyrGly: 1.808 ± 0.04	0.367TyrHis: 0.367 ± 0.016	0.341TyrIle: 0.341 ± 0.016	0.263TyrLys: 0.263 ± 0.013	1.968TyrLeu: 1.968 ± 0.043	0.178TyrMet: 0.178 ± 0.012	0.319TyrAsn: 0.319 ± 0.016	1.003TyrPro: 1.003 ± 0.025	0.421TyrGln: 0.421 ± 0.017	1.707TyrArg: 1.707 ± 0.033	0.815TyrSer: 0.815 ± 0.025	0.981TyrThr: 0.981 ± 0.027	1.429TyrVal: 1.429 ± 0.033	0.292TyrTrp: 0.292 ± 0.013	0.342TyrTyr: 0.342 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4780 proteins (1499939 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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