Bovine rotavirus C

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus C

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060NEB5|A0A060NEB5_9REOV Non-structural protein 5 OS=Bovine rotavirus C OX=31588 GN=NSP5 PE=3 SV=1
MM1 pKa = 7.33VCTTLYY7 pKa = 8.57TVCVVLCILLMYY19 pKa = 10.38ILLFRR24 pKa = 11.84KK25 pKa = 9.74VIHH28 pKa = 6.25LLIDD32 pKa = 3.23MSLIAFVISSCIGLSNAQFFANDD55 pKa = 3.36MLYY58 pKa = 10.69DD59 pKa = 3.91GNVEE63 pKa = 4.23GVINTTNIFNVEE75 pKa = 4.08SLCIYY80 pKa = 9.69FPNSAVGQPGPGKK93 pKa = 10.38SDD95 pKa = 3.68GLINDD100 pKa = 3.42NNYY103 pKa = 10.47AQTLAALFEE112 pKa = 4.7TKK114 pKa = 10.21GFPKK118 pKa = 10.62GSVNFNTYY126 pKa = 8.17TKK128 pKa = 10.27ISDD131 pKa = 4.65FINSIEE137 pKa = 4.2MTCSYY142 pKa = 10.65NIVIIPEE149 pKa = 3.94TLANSEE155 pKa = 4.34TIEE158 pKa = 4.51QVAEE162 pKa = 3.75WVLNVWKK169 pKa = 9.84CDD171 pKa = 3.2NMNVDD176 pKa = 3.42IYY178 pKa = 10.87TYY180 pKa = 10.33EE181 pKa = 3.99QVGKK185 pKa = 10.47DD186 pKa = 3.77NFWAAIGEE194 pKa = 4.39DD195 pKa = 4.29CDD197 pKa = 4.69VAVCPLDD204 pKa = 3.33TTMNGIGCTPASTEE218 pKa = 4.15TYY220 pKa = 9.71EE221 pKa = 4.26VLSNDD226 pKa = 3.46TQLALIDD233 pKa = 3.65VVDD236 pKa = 4.03NVKK239 pKa = 10.26HH240 pKa = 6.8RR241 pKa = 11.84IQLNQVTCKK250 pKa = 10.36LRR252 pKa = 11.84NCVKK256 pKa = 10.87GEE258 pKa = 3.72ARR260 pKa = 11.84LNTAIVRR267 pKa = 11.84ISNSSSFDD275 pKa = 3.44NSLSPLNNGQKK286 pKa = 8.7TRR288 pKa = 11.84SFKK291 pKa = 10.88INAKK295 pKa = 8.92KK296 pKa = 8.19WWKK299 pKa = 9.97IFYY302 pKa = 9.64TIIDD306 pKa = 4.23YY307 pKa = 10.94INTFIQSMTPRR318 pKa = 11.84HH319 pKa = 5.35RR320 pKa = 11.84AIYY323 pKa = 9.33PEE325 pKa = 3.79GWMLRR330 pKa = 11.84YY331 pKa = 9.45AA332 pKa = 4.23

Molecular weight: 37.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060NE87|A0A060NE87_9REOV RNA-directed RNA polymerase OS=Bovine rotavirus C OX=31588 GN=VP1 PE=3 SV=1
MM1 pKa = 7.5EE2 pKa = 6.6FINQTFFSEE11 pKa = 4.33SSEE14 pKa = 4.1SKK16 pKa = 9.93MDD18 pKa = 3.76VIPYY22 pKa = 10.04VLGIVLALTNGNRR35 pKa = 11.84VLKK38 pKa = 10.1IINFIITLFKK48 pKa = 11.09KK49 pKa = 9.65MVIAINVAISKK60 pKa = 8.74WKK62 pKa = 10.51RR63 pKa = 11.84EE64 pKa = 4.07NNDD67 pKa = 3.07VKK69 pKa = 11.18HH70 pKa = 5.05EE71 pKa = 4.19TKK73 pKa = 10.37DD74 pKa = 2.92IHH76 pKa = 8.3KK77 pKa = 9.91EE78 pKa = 3.81VEE80 pKa = 4.0EE81 pKa = 4.22VMTQIRR87 pKa = 11.84EE88 pKa = 4.04MRR90 pKa = 11.84IHH92 pKa = 6.57LTALFNSIHH101 pKa = 7.55DD102 pKa = 5.21DD103 pKa = 3.4NTKK106 pKa = 9.4WRR108 pKa = 11.84MSEE111 pKa = 3.9SIRR114 pKa = 11.84RR115 pKa = 11.84EE116 pKa = 3.92KK117 pKa = 10.18KK118 pKa = 10.33NEE120 pKa = 3.8MKK122 pKa = 10.69AHH124 pKa = 6.47AGVTTSKK131 pKa = 10.26QQLNNTSGLEE141 pKa = 3.99MEE143 pKa = 4.59VCLL146 pKa = 5.46

Molecular weight: 16.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5590

146

1090

508.2

58.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.136 ± 0.233

1.27 ± 0.354

5.975 ± 0.306

5.689 ± 0.335

4.436 ± 0.26

3.971 ± 0.414

1.807 ± 0.2

7.639 ± 0.295

6.655 ± 0.565

8.014 ± 0.473

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.47 ± 0.145

6.941 ± 0.346

2.916 ± 0.263

3.184 ± 0.415

4.741 ± 0.223

7.782 ± 0.512

6.369 ± 0.422

6.887 ± 0.379

1.127 ± 0.208

4.991 ± 0.505
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski