Helicobacter bizzozeronii CCUG 35545
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2512 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H5VEU7|H5VEU7_HELBI Flagellar FliJ protein OS=Helicobacter bizzozeronii CCUG 35545 OX=1002805 GN=HBZS_122270 PE=3 SV=1
MM1 pKa = 7.38 EE2 pKa = 5.74 AFIIDD7 pKa = 3.96 LWDD10 pKa = 3.72 KK11 pKa = 11.09 YY12 pKa = 10.75 AATWGYY18 pKa = 10.84 LILFFWSILEE28 pKa = 4.1 GEE30 pKa = 4.5 IGLILAGIASYY41 pKa = 10.25 AGHH44 pKa = 6.31 MNIFLAILVAGLGGFTGDD62 pKa = 4.46 QIYY65 pKa = 9.55 FLCGQDD71 pKa = 2.99
Molecular weight: 7.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.922
IPC2_protein 3.91
IPC_protein 3.706
Toseland 3.541
ProMoST 3.77
Dawson 3.719
Bjellqvist 4.024
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.465
Solomon 3.668
Lehninger 3.63
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.846
Patrickios 0.006
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.909
Protein with the highest isoelectric point:
>tr|H5VEK2|H5VEK2_HELBI F420_oxidored domain-containing protein OS=Helicobacter bizzozeronii CCUG 35545 OX=1002805 GN=HBZS_121320 PE=4 SV=1
MM1 pKa = 7.29 SAGNILYY8 pKa = 9.85 VFSSRR13 pKa = 11.84 RR14 pKa = 11.84 ACNAFCAQQEE24 pKa = 4.43 DD25 pKa = 4.92 GFLPTIWSVSEE36 pKa = 4.71 FYY38 pKa = 11.13 SQVSYY43 pKa = 11.34 VPHH46 pKa = 6.09 LQKK49 pKa = 10.74 NPRR52 pKa = 11.84 GRR54 pKa = 11.84 APSALNACDD63 pKa = 3.51 PGGARR68 pKa = 11.84 RR69 pKa = 11.84 GRR71 pKa = 11.84 FKK73 pKa = 10.88 RR74 pKa = 11.84 FRR76 pKa = 11.84 KK77 pKa = 9.66 LDD79 pKa = 4.25 RR80 pKa = 11.84 II81 pKa = 4.12
Molecular weight: 9.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.604
IPC_protein 10.423
Toseland 10.335
ProMoST 10.116
Dawson 10.526
Bjellqvist 10.292
Wikipedia 10.745
Rodwell 10.657
Grimsley 10.599
Solomon 10.613
Lehninger 10.57
Nozaki 10.394
DTASelect 10.262
Thurlkill 10.379
EMBOSS 10.745
Sillero 10.452
Patrickios 10.423
IPC_peptide 10.599
IPC2_peptide 9.619
IPC2.peptide.svr19 8.359
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2512
0
2512
517845
37
2004
206.1
23.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.767 ± 0.051
1.352 ± 0.027
4.713 ± 0.038
5.736 ± 0.066
4.937 ± 0.042
6.631 ± 0.06
2.782 ± 0.029
6.46 ± 0.045
7.084 ± 0.054
11.289 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.454 ± 0.026
4.345 ± 0.05
4.237 ± 0.04
4.921 ± 0.048
3.985 ± 0.036
6.276 ± 0.047
4.561 ± 0.048
5.971 ± 0.048
0.904 ± 0.019
3.594 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here