Mesorhizobium australicum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7PIS1|A0A1X7PIS1_9RHIZ 2-isopropylmalate synthase OS=Mesorhizobium australicum OX=536018 GN=leuA PE=3 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84LALAVLLAFAASPALAATCAPVSTEE27 pKa = 3.6ARR29 pKa = 11.84ILASVDD35 pKa = 3.19YY36 pKa = 11.15DD37 pKa = 4.97DD38 pKa = 5.89IHH40 pKa = 7.24PDD42 pKa = 2.77WSDD45 pKa = 2.96GSRR48 pKa = 11.84IGLSWSLDD56 pKa = 3.43LLGEE60 pKa = 4.22EE61 pKa = 4.56EE62 pKa = 5.57GEE64 pKa = 4.3DD65 pKa = 3.27GDD67 pKa = 4.37YY68 pKa = 11.44YY69 pKa = 11.34LIGDD73 pKa = 4.65LVDD76 pKa = 3.77PSGDD80 pKa = 3.33VVTEE84 pKa = 4.21SVFVSAMEE92 pKa = 4.16WEE94 pKa = 4.56CDD96 pKa = 3.2ADD98 pKa = 3.59

Molecular weight: 10.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7NDC0|A0A1X7NDC0_9RHIZ Uncharacterized protein OS=Mesorhizobium australicum OX=536018 GN=SAMN02982922_1627 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.15GGRR28 pKa = 11.84AVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5684

0

5684

1733231

38

2827

304.9

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.596 ± 0.045

0.795 ± 0.01

5.644 ± 0.026

5.817 ± 0.025

3.845 ± 0.021

8.669 ± 0.028

1.958 ± 0.013

5.365 ± 0.024

3.368 ± 0.025

9.811 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.016

2.538 ± 0.017

5.137 ± 0.025

2.823 ± 0.016

7.16 ± 0.032

5.465 ± 0.023

5.218 ± 0.02

7.586 ± 0.025

1.346 ± 0.012

2.259 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski