Lottiidibacillus patelloidae
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3053 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A263BT11|A0A263BT11_9BACI SelB translation factor OS=Lottiidibacillus patelloidae OX=2670334 GN=selB PE=4 SV=1
MM1 pKa = 7.2 KK2 pKa = 10.4 RR3 pKa = 11.84 KK4 pKa = 9.08 LALFLTFILLFSQSVFPLNRR24 pKa = 11.84 AFSEE28 pKa = 4.36 YY29 pKa = 10.74 GSNQIFSNVDD39 pKa = 3.0 WLPYY43 pKa = 10.54 AVGEE47 pKa = 4.49 GNDD50 pKa = 4.46 PLDD53 pKa = 3.78 QNPNSTDD60 pKa = 2.86 IAGNSEE66 pKa = 4.37 LPAVSYY72 pKa = 10.85 SYY74 pKa = 11.51 DD75 pKa = 3.16 STYY78 pKa = 11.03 LYY80 pKa = 11.11 FKK82 pKa = 10.14 MRR84 pKa = 11.84 LDD86 pKa = 4.7 ANPLNNKK93 pKa = 9.18 NDD95 pKa = 3.62 NFDD98 pKa = 3.33 QYY100 pKa = 11.94 AWTVLFNTDD109 pKa = 3.17 GNEE112 pKa = 4.05 HH113 pKa = 6.53 DD114 pKa = 3.76 WEE116 pKa = 4.61 YY117 pKa = 10.68 MVSLNGLAEE126 pKa = 4.11 TVQIGKK132 pKa = 8.33 NTDD135 pKa = 3.16 KK136 pKa = 11.19 EE137 pKa = 4.08 LDD139 pKa = 3.51 SWGDD143 pKa = 3.52 QAEE146 pKa = 4.29 DD147 pKa = 3.71 WLWTGPAIDD156 pKa = 4.55 YY157 pKa = 11.21 ADD159 pKa = 4.65 ALPIDD164 pKa = 3.98 VSLSNFNNDD173 pKa = 2.57 QDD175 pKa = 4.28 FFAYY179 pKa = 8.83 WAVPWTDD186 pKa = 4.18 FAAVTGLTPTSMIQLFYY203 pKa = 10.18 ATSANASNYY212 pKa = 10.07 NKK214 pKa = 10.29 DD215 pKa = 3.21 RR216 pKa = 11.84 MGGHH220 pKa = 6.3 PTSFADD226 pKa = 3.49 AWGEE230 pKa = 3.78 PILINGTTPNPPTIEE245 pKa = 3.94 IAGGDD250 pKa = 3.62 TKK252 pKa = 9.12 TTNDD256 pKa = 3.68 NTPLISGSSSAADD269 pKa = 3.63 GSFVDD274 pKa = 3.41 VTLYY278 pKa = 10.95 LKK280 pKa = 10.52 SDD282 pKa = 3.34 PTKK285 pKa = 9.78 TYY287 pKa = 9.41 TYY289 pKa = 8.85 QTTVEE294 pKa = 3.93 NGQWQTLVTTALNNGVYY311 pKa = 9.74 NVSAIVEE318 pKa = 4.03 EE319 pKa = 4.87 DD320 pKa = 3.56 GQFGIDD326 pKa = 3.69 EE327 pKa = 4.44 QEE329 pKa = 4.14 LTVDD333 pKa = 4.25 PSQIIAPEE341 pKa = 3.88 ITITTPSTGSAVTDD355 pKa = 3.51 STPTISGSYY364 pKa = 9.7 TSGTSTNNIVVEE376 pKa = 5.17 FINGNNVVATGSASLANGVWEE397 pKa = 4.33 FTPNSNLPLGTYY409 pKa = 7.15 QVKK412 pKa = 8.46 ATITDD417 pKa = 4.58 EE418 pKa = 4.05 INQTSTSSIEE428 pKa = 3.95 LSVAEE433 pKa = 4.48 AKK435 pKa = 10.32 VLNINIDD442 pKa = 3.85 TINPLVTNIVTPVFTGSSNAMDD464 pKa = 3.87 GVTIFVTINNTSYY477 pKa = 7.77 TTTVSNSSWSVQWPEE492 pKa = 3.56 ALAEE496 pKa = 4.11 GTYY499 pKa = 10.36 VVSASVEE506 pKa = 4.29 EE507 pKa = 4.21 QGQTASDD514 pKa = 4.49 SINLIVDD521 pKa = 3.56 TTKK524 pKa = 9.5 PTLSINGGTEE534 pKa = 3.49 RR535 pKa = 11.84 TTNEE539 pKa = 3.87 STPTISGTTDD549 pKa = 2.93 APDD552 pKa = 3.4 NSVVTITLNGKK563 pKa = 9.86 SYY565 pKa = 7.67 TTTVTDD571 pKa = 3.85 GTWTVDD577 pKa = 3.17 VGEE580 pKa = 4.6 LTDD583 pKa = 3.71 GQYY586 pKa = 11.2 EE587 pKa = 4.29 VTATITDD594 pKa = 3.65 EE595 pKa = 4.6 AGNTTTAKK603 pKa = 10.12 QSLTIDD609 pKa = 3.4 TTGPAIVITSPEE621 pKa = 3.61 NGAILNNHH629 pKa = 5.83 TPTVTGTVEE638 pKa = 3.81 AGSTVLVEE646 pKa = 5.89 FIDD649 pKa = 3.91 VNNSVVHH656 pKa = 6.25 SGHH659 pKa = 5.97 ATVTNEE665 pKa = 2.96 NWTYY669 pKa = 10.9 TPTGNLNEE677 pKa = 4.13 GTYY680 pKa = 10.13 SVKK683 pKa = 10.26 ATGTDD688 pKa = 3.07 ALGNPKK694 pKa = 8.84 STSITITIDD703 pKa = 2.93 VTAPTLTINGGTEE716 pKa = 3.59 RR717 pKa = 11.84 TTNDD721 pKa = 2.83 STPTVSGTTDD731 pKa = 3.2 APNGAIVTVTVNGQSYY747 pKa = 7.83 TATVTDD753 pKa = 4.2 GTWTVTVDD761 pKa = 3.27 EE762 pKa = 5.87 LIDD765 pKa = 3.42 GTYY768 pKa = 10.61 EE769 pKa = 3.8 VTATITDD776 pKa = 3.48 EE777 pKa = 4.48 AGNVSTKK784 pKa = 8.89 TQTLILSTIGPEE796 pKa = 4.05 LKK798 pKa = 9.69 IDD800 pKa = 3.88 SPEE803 pKa = 3.96 EE804 pKa = 3.75 GALLSNYY811 pKa = 9.64 HH812 pKa = 6.72 PIISGTTNGDD822 pKa = 2.94 KK823 pKa = 10.11 VTITIYY829 pKa = 11.52 DD830 pKa = 3.71 MLEE833 pKa = 4.18 KK834 pKa = 9.95 IIEE837 pKa = 4.3 TGVAEE842 pKa = 5.1 LDD844 pKa = 3.48 EE845 pKa = 5.29 DD846 pKa = 5.31 GNWTYY851 pKa = 10.5 NTVSSLSDD859 pKa = 3.07 GTYY862 pKa = 9.56 LAKK865 pKa = 10.63 AISTFNNGNDD875 pKa = 3.5 TEE877 pKa = 4.58 ASVSFIIDD885 pKa = 3.14 VTAPTLTIDD894 pKa = 3.69 GGTEE898 pKa = 3.51 RR899 pKa = 11.84 TTNDD903 pKa = 3.1 STPTISGTTDD913 pKa = 2.93 APDD916 pKa = 3.4 NSVVTITLNGKK927 pKa = 9.86 SYY929 pKa = 7.67 TTTVTDD935 pKa = 4.05 GTWTIDD941 pKa = 3.25 TDD943 pKa = 3.67 EE944 pKa = 4.77 LTDD947 pKa = 3.44 GTYY950 pKa = 10.84 EE951 pKa = 3.98 VTATITDD958 pKa = 3.61 EE959 pKa = 4.61 AGNSATAKK967 pKa = 10.04 QSLTIDD973 pKa = 3.42 TTGPTIAITSPEE985 pKa = 3.93 NGAVLNDD992 pKa = 3.43 QTPTVTGTVEE1002 pKa = 3.86 AGSTVIVEE1010 pKa = 5.91 FIDD1013 pKa = 3.63 EE1014 pKa = 4.32 NNSVVHH1020 pKa = 6.38 SGHH1023 pKa = 5.9 ATVANEE1029 pKa = 3.27 NWTYY1033 pKa = 10.91 TPTEE1037 pKa = 3.86 NLLEE1041 pKa = 4.1 GTYY1044 pKa = 7.93 TVKK1047 pKa = 10.32 ATGTDD1052 pKa = 3.09 ALGNPKK1058 pKa = 8.84 STSITITIDD1067 pKa = 2.93 VTAPTLTIDD1076 pKa = 3.96 GGAEE1080 pKa = 3.66 RR1081 pKa = 11.84 TTNDD1085 pKa = 2.82 STPTVSGTTDD1095 pKa = 3.04 APDD1098 pKa = 3.4 NSVVTITLNGKK1109 pKa = 9.86 SYY1111 pKa = 7.68 TTTVTNGTWTIDD1123 pKa = 3.23 VDD1125 pKa = 3.85 EE1126 pKa = 5.03 LTDD1129 pKa = 3.39 GTYY1132 pKa = 10.84 EE1133 pKa = 3.98 VTATITDD1140 pKa = 3.53 EE1141 pKa = 4.68 AGNSTTATQSLTIDD1155 pKa = 3.4 TLAPEE1160 pKa = 4.18 VSIDD1164 pKa = 3.61 NPLDD1168 pKa = 3.61 NSVLEE1173 pKa = 4.93 LGDD1176 pKa = 3.61 IPITGTADD1184 pKa = 3.03 VGSKK1188 pKa = 8.23 VTLVLDD1194 pKa = 4.22 DD1195 pKa = 4.88 NEE1197 pKa = 5.5 PITLTANEE1205 pKa = 4.07 NGEE1208 pKa = 4.59 WIYY1211 pKa = 11.03 HH1212 pKa = 5.08 PTPILSEE1219 pKa = 4.61 GIHH1222 pKa = 5.89 IVTVTAEE1229 pKa = 4.11 DD1230 pKa = 3.67 DD1231 pKa = 3.42 QGNVSEE1237 pKa = 4.65 PVSISFKK1244 pKa = 10.71 VVDD1247 pKa = 4.42 PNVIDD1252 pKa = 3.52 EE1253 pKa = 4.52 TEE1255 pKa = 3.86 INITIDD1261 pKa = 5.39 LSAQPQNILGNGIDD1275 pKa = 3.89 HH1276 pKa = 7.48 TILTAKK1282 pKa = 9.52 LTDD1285 pKa = 3.75 TNGEE1289 pKa = 3.92 PLVNYY1294 pKa = 7.1 EE1295 pKa = 4.54 VKK1297 pKa = 10.64 FSAPVGSFPEE1307 pKa = 4.24 GFIAFTDD1314 pKa = 3.47 QTGIATVKK1322 pKa = 10.01 YY1323 pKa = 9.64 VSAKK1327 pKa = 9.3 TEE1329 pKa = 3.93 SDD1331 pKa = 3.15 EE1332 pKa = 4.23 TLSYY1336 pKa = 10.66 LVSAKK1341 pKa = 10.42 AYY1343 pKa = 9.74 NLDD1346 pKa = 3.63 GNFVEE1351 pKa = 5.05 QEE1353 pKa = 3.98 QISISFNPAIISGVVIDD1370 pKa = 4.89 NEE1372 pKa = 4.51 TNEE1375 pKa = 4.94 PIHH1378 pKa = 5.79 NAKK1381 pKa = 9.97 VVLSSDD1387 pKa = 4.11 FNQDD1391 pKa = 2.45 GTIDD1395 pKa = 3.61 FTTTVLSDD1403 pKa = 3.39 VEE1405 pKa = 4.3 GKK1407 pKa = 10.31 YY1408 pKa = 10.95 VITIPNGNVSYY1419 pKa = 10.65 LLEE1422 pKa = 3.84 ITKK1425 pKa = 10.02 PVLIGNTMQDD1435 pKa = 3.03 VTFKK1439 pKa = 11.01 QSVTAGSVSGSGTDD1453 pKa = 3.12 EE1454 pKa = 4.64 FYY1456 pKa = 10.28 STEE1459 pKa = 4.04 SATGIILLSDD1469 pKa = 4.22 GSNGTLSLEE1478 pKa = 4.37 NYY1480 pKa = 10.05 DD1481 pKa = 4.08 SIIVEE1486 pKa = 4.33 VEE1488 pKa = 4.15 DD1489 pKa = 3.91 EE1490 pKa = 4.42 SGDD1493 pKa = 3.47 ITSLNGSVDD1502 pKa = 2.77 ARR1504 pKa = 11.84 TGVFLIDD1511 pKa = 3.57 GLDD1514 pKa = 3.42 VNKK1517 pKa = 10.64 AYY1519 pKa = 8.87 TLKK1522 pKa = 10.48 FYY1524 pKa = 11.22 YY1525 pKa = 10.57 VFEE1528 pKa = 5.78 DD1529 pKa = 4.09 GSKK1532 pKa = 9.88 IQVSSLAVVISNDD1545 pKa = 3.19 GEE1547 pKa = 4.51 LNISSCLIDD1556 pKa = 4.04 PYY1558 pKa = 11.08 GTITNKK1564 pKa = 8.52 EE1565 pKa = 4.09 TGEE1568 pKa = 4.24 VIEE1571 pKa = 4.38 GAVVEE1576 pKa = 5.23 LYY1578 pKa = 10.95 YY1579 pKa = 11.06 ADD1581 pKa = 3.36 TVRR1584 pKa = 11.84 NQEE1587 pKa = 3.75 NGRR1590 pKa = 11.84 VKK1592 pKa = 10.24 DD1593 pKa = 3.79 TLVEE1597 pKa = 4.03 LPILTFPPNDD1607 pKa = 2.97 NHH1609 pKa = 6.23 NPQNSSVEE1617 pKa = 3.87 GKK1619 pKa = 9.08 YY1620 pKa = 10.78 AYY1622 pKa = 8.92 MVYY1625 pKa = 10.44 PEE1627 pKa = 4.36 ADD1629 pKa = 3.41 YY1630 pKa = 11.49 YY1631 pKa = 10.73 ILVKK1635 pKa = 10.84 KK1636 pKa = 10.45 NGYY1639 pKa = 7.78 EE1640 pKa = 4.04 LYY1642 pKa = 9.12 EE1643 pKa = 4.45 SPVIEE1648 pKa = 4.22 VNQEE1652 pKa = 3.56 IVRR1655 pKa = 11.84 HH1656 pKa = 6.18 DD1657 pKa = 3.71 IEE1659 pKa = 4.71 LTPIPQEE1666 pKa = 4.18 LPATATSYY1674 pKa = 11.59 YY1675 pKa = 9.69 NYY1677 pKa = 10.47 LVTGVLLLFIGVLTIFLYY1695 pKa = 10.51 RR1696 pKa = 11.84 RR1697 pKa = 11.84 NRR1699 pKa = 11.84 YY1700 pKa = 9.85 SNN1702 pKa = 3.37
Molecular weight: 181.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.91
Patrickios 1.494
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A263BS86|A0A263BS86_9BACI Uncharacterized protein OS=Lottiidibacillus patelloidae OX=2670334 GN=CIB95_11750 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.02 KK14 pKa = 8.47 VHH16 pKa = 5.57 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSTKK25 pKa = 10.29 NGRR28 pKa = 11.84 KK29 pKa = 8.91 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3053
0
3053
875866
26
2026
286.9
32.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.496 ± 0.049
0.701 ± 0.013
5.066 ± 0.037
7.692 ± 0.051
4.697 ± 0.044
6.487 ± 0.046
2.052 ± 0.023
8.373 ± 0.044
7.528 ± 0.049
9.711 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.638 ± 0.022
5.042 ± 0.035
3.356 ± 0.031
3.358 ± 0.027
3.719 ± 0.032
5.95 ± 0.033
5.502 ± 0.037
6.936 ± 0.04
0.977 ± 0.018
3.719 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here