Stenotrophomonas sp. 278
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4280 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A427CDL5|A0A427CDL5_9GAMM Uncharacterized protein OS=Stenotrophomonas sp. 278 OX=2479851 GN=EGJ34_06475 PE=4 SV=1
MM1 pKa = 7.83 SDD3 pKa = 2.8 TAATTFRR10 pKa = 11.84 TWMCVVCGFIYY21 pKa = 10.51 RR22 pKa = 11.84 EE23 pKa = 3.74 VDD25 pKa = 3.29 GLPEE29 pKa = 4.0 EE30 pKa = 5.55 GIAPGTAWEE39 pKa = 4.8 DD40 pKa = 3.9 VPDD43 pKa = 3.5 TWTCPDD49 pKa = 3.91 CGVTKK54 pKa = 10.7 DD55 pKa = 3.73 DD56 pKa = 4.24 FEE58 pKa = 4.37 MVEE61 pKa = 3.83 IDD63 pKa = 3.32
Molecular weight: 7.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.811
IPC2_protein 3.617
IPC_protein 3.528
Toseland 3.338
ProMoST 3.643
Dawson 3.541
Bjellqvist 3.783
Wikipedia 3.503
Rodwell 3.376
Grimsley 3.261
Solomon 3.49
Lehninger 3.452
Nozaki 3.681
DTASelect 3.859
Thurlkill 3.414
EMBOSS 3.516
Sillero 3.656
Patrickios 0.604
IPC_peptide 3.49
IPC2_peptide 3.617
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A3R8U4R2|A0A3R8U4R2_9GAMM Phage major capsid protein OS=Stenotrophomonas sp. 278 OX=2479851 GN=EGJ34_06735 PE=4 SV=1
MM1 pKa = 7.05 ATKK4 pKa = 10.25 KK5 pKa = 10.11 AAKK8 pKa = 9.45 KK9 pKa = 10.34 KK10 pKa = 9.08 PAAKK14 pKa = 10.11 KK15 pKa = 9.84 AVKK18 pKa = 10.08 KK19 pKa = 9.48 VAKK22 pKa = 9.83 KK23 pKa = 9.89 AAAKK27 pKa = 8.8 KK28 pKa = 7.63 TVKK31 pKa = 10.28 KK32 pKa = 10.09 AAKK35 pKa = 9.64 KK36 pKa = 8.79 ATAKK40 pKa = 10.59 KK41 pKa = 10.02 AVKK44 pKa = 10.06 KK45 pKa = 9.45 VAKK48 pKa = 9.86 KK49 pKa = 9.61 AAARR53 pKa = 11.84 KK54 pKa = 8.88 AVKK57 pKa = 8.71 KK58 pKa = 7.65 TAKK61 pKa = 10.24 KK62 pKa = 10.08 AVKK65 pKa = 9.91 KK66 pKa = 9.52 VAKK69 pKa = 10.07 KK70 pKa = 9.29 ATAKK74 pKa = 10.36 KK75 pKa = 9.74 AAKK78 pKa = 8.5 KK79 pKa = 6.98 TAKK82 pKa = 10.07 KK83 pKa = 10.24 AVRR86 pKa = 11.84 KK87 pKa = 8.57 VAKK90 pKa = 10.1 KK91 pKa = 10.36 AGAKK95 pKa = 9.93 KK96 pKa = 10.13 AVKK99 pKa = 10.04 KK100 pKa = 9.79 VAKK103 pKa = 10.15 KK104 pKa = 10.19 SAAKK108 pKa = 8.65 KK109 pKa = 6.78 TAKK112 pKa = 10.15 KK113 pKa = 10.24 AVRR116 pKa = 11.84 KK117 pKa = 8.57 VAKK120 pKa = 10.1 KK121 pKa = 10.36 AGAKK125 pKa = 9.46 KK126 pKa = 10.17 AVRR129 pKa = 11.84 KK130 pKa = 8.95 VAKK133 pKa = 10.3 KK134 pKa = 9.43 PAAKK138 pKa = 9.88 KK139 pKa = 10.37 ASAKK143 pKa = 10.32 KK144 pKa = 10.01 AVKK147 pKa = 9.96 KK148 pKa = 9.09 VAKK151 pKa = 9.85 RR152 pKa = 11.84 KK153 pKa = 8.17 PAVRR157 pKa = 11.84 KK158 pKa = 10.0 KK159 pKa = 10.1 KK160 pKa = 9.79 AAPVALPATPAPLII174 pKa = 4.24
Molecular weight: 18.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.384
IPC2_protein 11.33
IPC_protein 12.544
Toseland 12.866
ProMoST 13.217
Dawson 12.881
Bjellqvist 12.764
Wikipedia 13.247
Rodwell 13.378
Grimsley 12.896
Solomon 13.261
Lehninger 13.173
Nozaki 12.852
DTASelect 12.764
Thurlkill 12.852
EMBOSS 13.32
Sillero 12.852
Patrickios 13.086
IPC_peptide 13.276
IPC2_peptide 12.193
IPC2.peptide.svr19 8.87
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4280
0
4280
1357534
25
4138
317.2
34.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.535 ± 0.055
0.786 ± 0.013
5.742 ± 0.027
5.266 ± 0.034
3.302 ± 0.023
8.483 ± 0.045
2.206 ± 0.023
4.049 ± 0.03
2.786 ± 0.038
10.766 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.212 ± 0.02
2.739 ± 0.027
5.299 ± 0.031
4.268 ± 0.028
7.255 ± 0.042
5.5 ± 0.033
5.259 ± 0.044
7.648 ± 0.031
1.56 ± 0.018
2.338 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here