Eptesicus serotinus papillomavirus 1
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8EH50|W8EH50_9PAPI Minor capsid protein L2 OS=Eptesicus serotinus papillomavirus 1 OX=1464071 GN=L2 PE=3 SV=1
MM1 pKa = 6.92 VVVLRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 9.69 RR10 pKa = 11.84 DD11 pKa = 3.34 TVEE14 pKa = 3.89 NIYY17 pKa = 9.95 RR18 pKa = 11.84 QCIRR22 pKa = 11.84 FGTCPPDD29 pKa = 3.47 VKK31 pKa = 11.2 NKK33 pKa = 10.07 VEE35 pKa = 4.06 NNTLADD41 pKa = 4.04 KK42 pKa = 10.55 ILRR45 pKa = 11.84 YY46 pKa = 10.17 GSGAVYY52 pKa = 10.26 FGGLGIGTGTGRR64 pKa = 11.84 GTTGVSVGRR73 pKa = 11.84 QVPVRR78 pKa = 11.84 PFRR81 pKa = 11.84 PHH83 pKa = 6.83 GPIDD87 pKa = 3.61 TIGIEE92 pKa = 4.26 NIGPRR97 pKa = 11.84 EE98 pKa = 4.08 TPFAPPEE105 pKa = 4.02 RR106 pKa = 11.84 APVDD110 pKa = 3.33 VDD112 pKa = 3.89 LGTGHH117 pKa = 6.6 VEE119 pKa = 3.83 PSDD122 pKa = 3.59 PSVIEE127 pKa = 4.09 PTDD130 pKa = 3.86 LPIDD134 pKa = 3.82 IPTSSVDD141 pKa = 3.5 EE142 pKa = 4.81 VIVIPTPEE150 pKa = 3.67 ITIGGDD156 pKa = 2.93 TTAIIEE162 pKa = 4.38 VTPSVPEE169 pKa = 3.61 NTVISRR175 pKa = 11.84 TQYY178 pKa = 9.63 TNPAFDD184 pKa = 3.59 ISLFSDD190 pKa = 3.52 STSGEE195 pKa = 4.02 LSASDD200 pKa = 4.76 HH201 pKa = 5.87 ITVSGGGGTIVGGHH215 pKa = 5.94 YY216 pKa = 10.21 PLAVPEE222 pKa = 4.48 GEE224 pKa = 4.58 EE225 pKa = 3.74 IPLAEE230 pKa = 4.74 FSPSVSRR237 pKa = 11.84 FRR239 pKa = 11.84 PGQVTEE245 pKa = 4.0 QEE247 pKa = 4.15 EE248 pKa = 4.77 TSFITSTPEE257 pKa = 3.42 GARR260 pKa = 11.84 ARR262 pKa = 11.84 PGRR265 pKa = 11.84 TRR267 pKa = 11.84 PSLYY271 pKa = 9.94 GRR273 pKa = 11.84 RR274 pKa = 11.84 IEE276 pKa = 4.11 QVEE279 pKa = 3.98 ITDD282 pKa = 4.12 PLFIDD287 pKa = 3.64 EE288 pKa = 4.87 PGRR291 pKa = 11.84 LVEE294 pKa = 4.54 FNYY297 pKa = 10.19 VNPAFEE303 pKa = 4.63 GDD305 pKa = 2.87 ITEE308 pKa = 4.78 IFEE311 pKa = 4.75 QDD313 pKa = 3.03 ILDD316 pKa = 3.98 EE317 pKa = 4.41 ARR319 pKa = 11.84 AAPSSAFQDD328 pKa = 3.24 IVRR331 pKa = 11.84 LGRR334 pKa = 11.84 QQLSRR339 pKa = 11.84 SRR341 pKa = 11.84 SGLVRR346 pKa = 11.84 VSRR349 pKa = 11.84 LGQRR353 pKa = 11.84 GTIRR357 pKa = 11.84 TRR359 pKa = 11.84 SGVRR363 pKa = 11.84 IGSTTHH369 pKa = 6.92 FYY371 pKa = 10.55 MDD373 pKa = 5.02 LSPIGADD380 pKa = 2.85 TGIPIEE386 pKa = 4.33 PPGEE390 pKa = 3.98 QSLEE394 pKa = 3.87 ASIVQPFSEE403 pKa = 4.32 EE404 pKa = 4.24 GYY406 pKa = 11.0 EE407 pKa = 4.3 LVNLNDD413 pKa = 3.4 VSGVVPDD420 pKa = 4.17 EE421 pKa = 4.51 SLLDD425 pKa = 3.78 SYY427 pKa = 11.77 EE428 pKa = 3.83 EE429 pKa = 4.4 TEE431 pKa = 5.28 FIGSDD436 pKa = 3.65 LQLVIHH442 pKa = 7.28 DD443 pKa = 4.37 EE444 pKa = 5.1 DD445 pKa = 3.94 IGIDD449 pKa = 3.61 QDD451 pKa = 3.45 SDD453 pKa = 3.95 TVIDD457 pKa = 3.91 IPSGLVKK464 pKa = 10.54 KK465 pKa = 10.57 AGPYY469 pKa = 10.17 YY470 pKa = 9.98 PDD472 pKa = 3.26 IDD474 pKa = 3.86 FAGVNIDD481 pKa = 3.35 YY482 pKa = 10.4 DD483 pKa = 3.69 KK484 pKa = 11.45 SHH486 pKa = 7.13 TGRR489 pKa = 11.84 AGDD492 pKa = 4.07 IVPSDD497 pKa = 3.86 TPIVTLDD504 pKa = 2.68 IWSSDD509 pKa = 3.63 FYY511 pKa = 11.06 LHH513 pKa = 7.01 PSLRR517 pKa = 11.84 RR518 pKa = 11.84 KK519 pKa = 9.13 RR520 pKa = 11.84 RR521 pKa = 11.84 RR522 pKa = 11.84 RR523 pKa = 11.84 RR524 pKa = 11.84 KK525 pKa = 9.39 YY526 pKa = 8.78 VFVYY530 pKa = 10.68
Molecular weight: 57.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.729
IPC2_protein 4.673
IPC_protein 4.635
Toseland 4.482
ProMoST 4.736
Dawson 4.584
Bjellqvist 4.724
Wikipedia 4.457
Rodwell 4.469
Grimsley 4.38
Solomon 4.584
Lehninger 4.533
Nozaki 4.685
DTASelect 4.863
Thurlkill 4.482
EMBOSS 4.469
Sillero 4.749
Patrickios 4.075
IPC_peptide 4.584
IPC2_peptide 4.736
IPC2.peptide.svr19 4.726
Protein with the highest isoelectric point:
>tr|W8EAA5|W8EAA5_9PAPI Replication protein E1 OS=Eptesicus serotinus papillomavirus 1 OX=1464071 GN=E1 PE=3 SV=1
MM1 pKa = 7.82 SEE3 pKa = 4.01 LQSRR7 pKa = 11.84 LDD9 pKa = 3.9 SIQDD13 pKa = 3.6 HH14 pKa = 6.06 ILEE17 pKa = 4.34 LFEE20 pKa = 5.12 RR21 pKa = 11.84 QSTDD25 pKa = 3.42 LKK27 pKa = 11.17 DD28 pKa = 3.78 HH29 pKa = 5.5 EE30 pKa = 4.91 QYY32 pKa = 10.2 WLLIRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 3.85 QAILFLARR47 pKa = 11.84 QKK49 pKa = 10.49 HH50 pKa = 5.07 VRR52 pKa = 11.84 LSMPVPSQAASKK64 pKa = 10.62 SRR66 pKa = 11.84 ARR68 pKa = 11.84 DD69 pKa = 3.74 AIEE72 pKa = 3.68 MSLLIKK78 pKa = 10.46 GLSEE82 pKa = 4.01 SQYY85 pKa = 9.96 GTEE88 pKa = 4.18 EE89 pKa = 3.63 WTLQQVSRR97 pKa = 11.84 EE98 pKa = 3.91 RR99 pKa = 11.84 LLAPPEE105 pKa = 3.92 YY106 pKa = 9.49 TFKK109 pKa = 11.02 KK110 pKa = 8.9 GGRR113 pKa = 11.84 PITVMFDD120 pKa = 3.52 DD121 pKa = 4.81 MPDD124 pKa = 3.04 NTAEE128 pKa = 3.95 YY129 pKa = 8.52 TAWDD133 pKa = 4.66 LIYY136 pKa = 11.08 YY137 pKa = 9.96 QDD139 pKa = 6.11 ADD141 pKa = 4.07 NQWQKK146 pKa = 11.7 AKK148 pKa = 10.81 GDD150 pKa = 3.41 VDD152 pKa = 3.91 MEE154 pKa = 4.24 GLFYY158 pKa = 10.73 KK159 pKa = 10.55 DD160 pKa = 3.43 SEE162 pKa = 4.41 GDD164 pKa = 2.98 KK165 pKa = 10.81 VYY167 pKa = 11.08 YY168 pKa = 10.56 VDD170 pKa = 5.19 FSRR173 pKa = 11.84 EE174 pKa = 3.68 ATKK177 pKa = 10.75 YY178 pKa = 10.31 GRR180 pKa = 11.84 TGKK183 pKa = 9.58 WSLLYY188 pKa = 10.85 NNNALASVDD197 pKa = 3.63 SPGYY201 pKa = 9.64 RR202 pKa = 11.84 DD203 pKa = 3.87 SDD205 pKa = 4.14 TEE207 pKa = 4.18 SSPDD211 pKa = 3.49 SSPVPARR218 pKa = 11.84 SSRR221 pKa = 11.84 NSKK224 pKa = 10.11 SRR226 pKa = 11.84 SPAKK230 pKa = 9.37 DD231 pKa = 2.85 TRR233 pKa = 11.84 RR234 pKa = 11.84 RR235 pKa = 11.84 PRR237 pKa = 11.84 LWLRR241 pKa = 11.84 SKK243 pKa = 10.53 SRR245 pKa = 11.84 SRR247 pKa = 11.84 SRR249 pKa = 11.84 SRR251 pKa = 11.84 SSSRR255 pKa = 11.84 SSGRR259 pKa = 11.84 SRR261 pKa = 11.84 SRR263 pKa = 11.84 SPFRR267 pKa = 11.84 RR268 pKa = 11.84 GASTVLTEE276 pKa = 4.06 RR277 pKa = 11.84 TRR279 pKa = 11.84 RR280 pKa = 11.84 SPGSHH285 pKa = 5.86 LQSPGSPVSSTSAGRR300 pKa = 11.84 RR301 pKa = 11.84 GSAGPRR307 pKa = 11.84 RR308 pKa = 11.84 LGSSSRR314 pKa = 11.84 PRR316 pKa = 11.84 EE317 pKa = 3.85 LAPDD321 pKa = 3.41 QVGSSRR327 pKa = 11.84 QTVSSRR333 pKa = 11.84 PRR335 pKa = 11.84 SSLEE339 pKa = 3.61 RR340 pKa = 11.84 LLEE343 pKa = 4.03 EE344 pKa = 5.07 ARR346 pKa = 11.84 DD347 pKa = 3.95 PPGLLLTGPPNTLKK361 pKa = 10.7 CYY363 pKa = 10.22 RR364 pKa = 11.84 YY365 pKa = 9.78 SLKK368 pKa = 10.61 NKK370 pKa = 9.53 HH371 pKa = 6.26 SSLFTLISTTFHH383 pKa = 5.1 WTEE386 pKa = 3.71 SKK388 pKa = 10.44 GPKK391 pKa = 9.7 RR392 pKa = 11.84 IGDD395 pKa = 3.57 GKK397 pKa = 9.41 MVIMFDD403 pKa = 4.46 DD404 pKa = 3.93 EE405 pKa = 4.58 AARR408 pKa = 11.84 EE409 pKa = 4.19 WFLKK413 pKa = 9.54 TVSLPRR419 pKa = 11.84 TLAAVKK425 pKa = 10.5 VDD427 pKa = 4.08 FGGLL431 pKa = 3.32
Molecular weight: 48.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.227
IPC2_protein 9.399
IPC_protein 9.706
Toseland 10.189
ProMoST 9.882
Dawson 10.365
Bjellqvist 10.043
Wikipedia 10.555
Rodwell 10.643
Grimsley 10.438
Solomon 10.409
Lehninger 10.379
Nozaki 10.16
DTASelect 10.043
Thurlkill 10.233
EMBOSS 10.599
Sillero 10.277
Patrickios 10.218
IPC_peptide 10.409
IPC2_peptide 8.668
IPC2.peptide.svr19 8.591
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2370
105
642
395.0
44.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.696 ± 0.58
1.646 ± 0.73
6.667 ± 0.338
6.287 ± 0.548
4.852 ± 0.568
6.54 ± 0.81
1.857 ± 0.211
4.979 ± 1.071
4.684 ± 0.959
8.776 ± 0.944
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.857 ± 0.38
3.713 ± 0.691
5.907 ± 1.01
4.262 ± 0.427
7.257 ± 1.072
8.692 ± 1.348
5.907 ± 0.43
6.371 ± 0.838
1.266 ± 0.333
2.785 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here