Psychrobacillus sp. OK032

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Psychrobacillus; unclassified Psychrobacillus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4290 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9UPD9|A0A1H9UPD9_9BACI Spore germination protein KB OS=Psychrobacillus sp. OK032 OX=1884358 GN=SAMN05518872_104247 PE=3 SV=1
MM1 pKa = 7.71TINDD5 pKa = 4.56AIFGEE10 pKa = 4.3LEE12 pKa = 3.9YY13 pKa = 11.21DD14 pKa = 4.03FGWSRR19 pKa = 11.84VITIDD24 pKa = 3.3FFGNATEE31 pKa = 4.36IDD33 pKa = 4.41LIIDD37 pKa = 3.87GEE39 pKa = 4.12EE40 pKa = 4.03DD41 pKa = 3.61GQFDD45 pKa = 3.68EE46 pKa = 5.15GQYY49 pKa = 9.17TAYY52 pKa = 10.88NSLRR56 pKa = 11.84GNWNDD61 pKa = 3.76LQLNLLNSILTYY73 pKa = 10.0YY74 pKa = 9.64NKK76 pKa = 9.78KK77 pKa = 9.77RR78 pKa = 11.84QEE80 pKa = 3.82LGYY83 pKa = 10.61DD84 pKa = 2.94IEE86 pKa = 5.19LNEE89 pKa = 4.42NYY91 pKa = 9.97PVVEE95 pKa = 4.21TTNQILEE102 pKa = 4.39MISLDD107 pKa = 4.2GIVVPYY113 pKa = 11.04ADD115 pKa = 3.26IFEE118 pKa = 4.49ARR120 pKa = 11.84HH121 pKa = 5.36IGITFNCTWDD131 pKa = 3.72TEE133 pKa = 4.38NGLGLRR139 pKa = 11.84LLDD142 pKa = 3.8EE143 pKa = 4.83KK144 pKa = 10.45VTEE147 pKa = 4.01VGYY150 pKa = 10.87QDD152 pKa = 3.56VAII155 pKa = 4.79

Molecular weight: 17.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9X5T7|A0A1H9X5T7_9BACI Adenylosuccinate synthetase OS=Psychrobacillus sp. OK032 OX=1884358 GN=purA PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.55RR3 pKa = 11.84TFQPNSRR10 pKa = 11.84KK11 pKa = 9.32RR12 pKa = 11.84AKK14 pKa = 10.19NHH16 pKa = 4.65GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.66NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4290

0

4290

1198288

28

1738

279.3

31.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.172 ± 0.039

0.643 ± 0.011

4.957 ± 0.031

7.593 ± 0.046

4.629 ± 0.029

6.776 ± 0.038

1.989 ± 0.02

8.251 ± 0.043

6.746 ± 0.031

9.682 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.019

4.523 ± 0.025

3.517 ± 0.021

3.627 ± 0.025

3.75 ± 0.028

6.069 ± 0.029

5.698 ± 0.027

7.168 ± 0.029

0.984 ± 0.015

3.434 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski