Amino acid dipepetide frequency for Psychrobacillus sp. OK032

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.737AlaAla: 5.737 ± 0.101	0.572AlaCys: 0.572 ± 0.025	3.364AlaAsp: 3.364 ± 0.058	4.783AlaGlu: 4.783 ± 0.078	3.497AlaPhe: 3.497 ± 0.052	5.25AlaGly: 5.25 ± 0.071	1.284AlaHis: 1.284 ± 0.036	6.527AlaIle: 6.527 ± 0.089	4.853AlaLys: 4.853 ± 0.074	7.173AlaLeu: 7.173 ± 0.084	2.05AlaMet: 2.05 ± 0.048	3.122AlaAsn: 3.122 ± 0.062	2.092AlaPro: 2.092 ± 0.047	2.243AlaGln: 2.243 ± 0.052	2.524AlaArg: 2.524 ± 0.046	4.304AlaSer: 4.304 ± 0.061	4.012AlaThr: 4.012 ± 0.06	5.32AlaVal: 5.32 ± 0.061	0.594AlaTrp: 0.594 ± 0.021	2.414AlaTyr: 2.414 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.425CysAla: 0.425 ± 0.019	0.071CysCys: 0.071 ± 0.008	0.36CysAsp: 0.36 ± 0.019	0.401CysGlu: 0.401 ± 0.019	0.281CysPhe: 0.281 ± 0.016	0.582CysGly: 0.582 ± 0.026	0.163CysHis: 0.163 ± 0.012	0.535CysIle: 0.535 ± 0.023	0.346CysLys: 0.346 ± 0.017	0.589CysLeu: 0.589 ± 0.024	0.148CysMet: 0.148 ± 0.011	0.268CysAsn: 0.268 ± 0.014	0.305CysPro: 0.305 ± 0.017	0.192CysGln: 0.192 ± 0.013	0.265CysArg: 0.265 ± 0.016	0.453CysSer: 0.453 ± 0.022	0.39CysThr: 0.39 ± 0.021	0.385CysVal: 0.385 ± 0.019	0.068CysTrp: 0.068 ± 0.007	0.206CysTyr: 0.206 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.644AspAla: 3.644 ± 0.063	0.319AspCys: 0.319 ± 0.017	2.365AspAsp: 2.365 ± 0.05	4.302AspGlu: 4.302 ± 0.064	2.518AspPhe: 2.518 ± 0.049	3.319AspGly: 3.319 ± 0.055	0.99AspHis: 0.99 ± 0.03	4.181AspIle: 4.181 ± 0.063	2.988AspLys: 2.988 ± 0.046	4.778AspLeu: 4.778 ± 0.069	1.395AspMet: 1.395 ± 0.036	1.829AspAsn: 1.829 ± 0.038	1.81AspPro: 1.81 ± 0.039	1.704AspGln: 1.704 ± 0.034	1.907AspArg: 1.907 ± 0.039	2.704AspSer: 2.704 ± 0.047	2.519AspThr: 2.519 ± 0.048	3.716AspVal: 3.716 ± 0.057	0.588AspTrp: 0.588 ± 0.024	1.994AspTyr: 1.994 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.471GluAla: 5.471 ± 0.076	0.351GluCys: 0.351 ± 0.018	3.749GluAsp: 3.749 ± 0.06	7.28GluGlu: 7.28 ± 0.108	2.631GluPhe: 2.631 ± 0.047	4.269GluGly: 4.269 ± 0.068	1.454GluHis: 1.454 ± 0.035	5.922GluIle: 5.922 ± 0.077	6.479GluLys: 6.479 ± 0.094	7.184GluLeu: 7.184 ± 0.083	2.307GluMet: 2.307 ± 0.044	3.814GluAsn: 3.814 ± 0.062	1.906GluPro: 1.906 ± 0.045	3.603GluGln: 3.603 ± 0.058	3.296GluArg: 3.296 ± 0.056	3.686GluSer: 3.686 ± 0.056	4.015GluThr: 4.015 ± 0.065	5.45GluVal: 5.45 ± 0.081	0.856GluTrp: 0.856 ± 0.03	2.21GluTyr: 2.21 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.233PheAla: 3.233 ± 0.053	0.308PheCys: 0.308 ± 0.017	2.382PheAsp: 2.382 ± 0.05	3.085PheGlu: 3.085 ± 0.045	2.346PhePhe: 2.346 ± 0.058	3.453PheGly: 3.453 ± 0.056	1.007PheHis: 1.007 ± 0.027	4.051PheIle: 4.051 ± 0.074	2.338PheLys: 2.338 ± 0.042	4.411PheLeu: 4.411 ± 0.074	1.185PheMet: 1.185 ± 0.032	1.973PheAsn: 1.973 ± 0.044	1.704PhePro: 1.704 ± 0.04	1.626PheGln: 1.626 ± 0.038	1.443PheArg: 1.443 ± 0.033	3.367PheSer: 3.367 ± 0.056	2.756PheThr: 2.756 ± 0.042	3.427PheVal: 3.427 ± 0.053	0.482PheTrp: 0.482 ± 0.025	1.712PheTyr: 1.712 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	5.003GlyAla: 5.003 ± 0.078	0.536GlyCys: 0.536 ± 0.026	3.156GlyAsp: 3.156 ± 0.048	4.462GlyGlu: 4.462 ± 0.063	3.369GlyPhe: 3.369 ± 0.059	4.685GlyGly: 4.685 ± 0.085	1.302GlyHis: 1.302 ± 0.03	6.198GlyIle: 6.198 ± 0.085	4.854GlyLys: 4.854 ± 0.065	6.368GlyLeu: 6.368 ± 0.08	2.045GlyMet: 2.045 ± 0.043	2.801GlyAsn: 2.801 ± 0.045	1.713GlyPro: 1.713 ± 0.04	2.236GlyGln: 2.236 ± 0.052	2.457GlyArg: 2.457 ± 0.043	3.978GlySer: 3.978 ± 0.057	4.102GlyThr: 4.102 ± 0.073	5.005GlyVal: 5.005 ± 0.069	0.722GlyTrp: 0.722 ± 0.021	2.767GlyTyr: 2.767 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.397HisAla: 1.397 ± 0.034	0.164HisCys: 0.164 ± 0.012	0.949HisAsp: 0.949 ± 0.029	1.369HisGlu: 1.369 ± 0.035	1.04HisPhe: 1.04 ± 0.036	1.312HisGly: 1.312 ± 0.037	0.563HisHis: 0.563 ± 0.025	1.58HisIle: 1.58 ± 0.038	0.98HisLys: 0.98 ± 0.024	2.038HisLeu: 2.038 ± 0.052	0.522HisMet: 0.522 ± 0.019	0.727HisAsn: 0.727 ± 0.026	1.078HisPro: 1.078 ± 0.034	0.745HisGln: 0.745 ± 0.028	0.797HisArg: 0.797 ± 0.027	1.178HisSer: 1.178 ± 0.031	1.08HisThr: 1.08 ± 0.03	1.383HisVal: 1.383 ± 0.031	0.186HisTrp: 0.186 ± 0.012	0.804HisTyr: 0.804 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.664IleAla: 6.664 ± 0.067	0.622IleCys: 0.622 ± 0.025	4.451IleAsp: 4.451 ± 0.058	6.144IleGlu: 6.144 ± 0.067	3.506IlePhe: 3.506 ± 0.072	6.256IleGly: 6.256 ± 0.083	1.718IleHis: 1.718 ± 0.036	6.871IleIle: 6.871 ± 0.11	4.36IleLys: 4.36 ± 0.068	7.466IleLeu: 7.466 ± 0.1	1.929IleMet: 1.929 ± 0.041	3.307IleAsn: 3.307 ± 0.064	3.512IlePro: 3.512 ± 0.06	3.171IleGln: 3.171 ± 0.053	3.152IleArg: 3.152 ± 0.053	5.265IleSer: 5.265 ± 0.066	4.697IleThr: 4.697 ± 0.068	6.278IleVal: 6.278 ± 0.072	0.704IleTrp: 0.704 ± 0.024	2.442IleTyr: 2.442 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.358LysAla: 4.358 ± 0.075	0.309LysCys: 0.309 ± 0.017	3.563LysAsp: 3.563 ± 0.055	6.811LysGlu: 6.811 ± 0.077	2.12LysPhe: 2.12 ± 0.042	4.198LysGly: 4.198 ± 0.063	1.25LysHis: 1.25 ± 0.029	4.679LysIle: 4.679 ± 0.064	5.516LysLys: 5.516 ± 0.073	5.864LysLeu: 5.864 ± 0.076	2.228LysMet: 2.228 ± 0.039	3.263LysAsn: 3.263 ± 0.052	2.089LysPro: 2.089 ± 0.04	2.97LysGln: 2.97 ± 0.049	3.053LysArg: 3.053 ± 0.053	3.619LysSer: 3.619 ± 0.052	3.774LysThr: 3.774 ± 0.054	4.739LysVal: 4.739 ± 0.06	0.895LysTrp: 0.895 ± 0.028	2.164LysTyr: 2.164 ± 0.044	0.0LysXaa: 0.0 ± 0.0
Leu	7.32LeuAla: 7.32 ± 0.082	0.577LeuCys: 0.577 ± 0.022	4.728LeuAsp: 4.728 ± 0.056	6.855LeuGlu: 6.855 ± 0.094	4.913LeuPhe: 4.913 ± 0.082	6.311LeuGly: 6.311 ± 0.076	1.958LeuHis: 1.958 ± 0.044	7.24LeuIle: 7.24 ± 0.109	6.247LeuLys: 6.247 ± 0.077	10.216LeuLeu: 10.216 ± 0.128	2.533LeuMet: 2.533 ± 0.045	4.219LeuAsn: 4.219 ± 0.059	3.815LeuPro: 3.815 ± 0.054	3.692LeuGln: 3.692 ± 0.061	3.504LeuArg: 3.504 ± 0.053	6.634LeuSer: 6.634 ± 0.074	5.698LeuThr: 5.698 ± 0.087	6.56LeuVal: 6.56 ± 0.081	0.784LeuTrp: 0.784 ± 0.025	3.019LeuTyr: 3.019 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	1.932MetAla: 1.932 ± 0.039	0.136MetCys: 0.136 ± 0.011	1.475MetAsp: 1.475 ± 0.041	2.136MetGlu: 2.136 ± 0.042	1.04MetPhe: 1.04 ± 0.031	1.749MetGly: 1.749 ± 0.047	0.515MetHis: 0.515 ± 0.023	2.149MetIle: 2.149 ± 0.048	2.498MetLys: 2.498 ± 0.042	2.59MetLeu: 2.59 ± 0.05	0.876MetMet: 0.876 ± 0.043	1.602MetAsn: 1.602 ± 0.038	0.991MetPro: 0.991 ± 0.031	1.004MetGln: 1.004 ± 0.028	1.058MetArg: 1.058 ± 0.025	1.702MetSer: 1.702 ± 0.04	1.811MetThr: 1.811 ± 0.033	1.613MetVal: 1.613 ± 0.039	0.208MetTrp: 0.208 ± 0.014	0.809MetTyr: 0.809 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.123AsnAla: 3.123 ± 0.063	0.317AsnCys: 0.317 ± 0.017	2.222AsnAsp: 2.222 ± 0.045	3.718AsnGlu: 3.718 ± 0.053	1.81AsnPhe: 1.81 ± 0.043	3.429AsnGly: 3.429 ± 0.06	0.952AsnHis: 0.952 ± 0.032	3.616AsnIle: 3.616 ± 0.051	2.968AsnLys: 2.968 ± 0.06	3.919AsnLeu: 3.919 ± 0.052	1.213AsnMet: 1.213 ± 0.032	2.196AsnAsn: 2.196 ± 0.047	2.085AsnPro: 2.085 ± 0.044	1.556AsnGln: 1.556 ± 0.039	1.964AsnArg: 1.964 ± 0.044	2.531AsnSer: 2.531 ± 0.054	2.375AsnThr: 2.375 ± 0.048	3.114AsnVal: 3.114 ± 0.053	0.521AsnTrp: 0.521 ± 0.02	1.602AsnTyr: 1.602 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	2.228ProAla: 2.228 ± 0.048	0.195ProCys: 0.195 ± 0.014	1.706ProAsp: 1.706 ± 0.036	2.752ProGlu: 2.752 ± 0.052	2.031ProPhe: 2.031 ± 0.044	2.227ProGly: 2.227 ± 0.053	0.746ProHis: 0.746 ± 0.028	3.134ProIle: 3.134 ± 0.048	2.222ProLys: 2.222 ± 0.043	3.376ProLeu: 3.376 ± 0.056	0.839ProMet: 0.839 ± 0.026	1.774ProAsn: 1.774 ± 0.043	0.9ProPro: 0.9 ± 0.031	1.087ProGln: 1.087 ± 0.027	0.981ProArg: 0.981 ± 0.027	2.26ProSer: 2.26 ± 0.039	2.192ProThr: 2.192 ± 0.043	2.829ProVal: 2.829 ± 0.051	0.338ProTrp: 0.338 ± 0.018	1.351ProTyr: 1.351 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.691GlnAla: 2.691 ± 0.052	0.181GlnCys: 0.181 ± 0.012	1.556GlnAsp: 1.556 ± 0.036	2.563GlnGlu: 2.563 ± 0.054	1.595GlnPhe: 1.595 ± 0.038	2.013GlnGly: 2.013 ± 0.05	0.756GlnHis: 0.756 ± 0.024	2.703GlnIle: 2.703 ± 0.048	2.68GlnLys: 2.68 ± 0.05	4.204GlnLeu: 4.204 ± 0.068	1.131GlnMet: 1.131 ± 0.034	1.642GlnAsn: 1.642 ± 0.04	1.127GlnPro: 1.127 ± 0.032	1.75GlnGln: 1.75 ± 0.044	1.28GlnArg: 1.28 ± 0.034	2.17GlnSer: 2.17 ± 0.044	2.122GlnThr: 2.122 ± 0.042	2.503GlnVal: 2.503 ± 0.05	0.346GlnTrp: 0.346 ± 0.017	1.255GlnTyr: 1.255 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.425ArgAla: 2.425 ± 0.05	0.224ArgCys: 0.224 ± 0.014	1.919ArgAsp: 1.919 ± 0.041	2.902ArgGlu: 2.902 ± 0.053	1.793ArgPhe: 1.793 ± 0.037	2.194ArgGly: 2.194 ± 0.051	0.723ArgHis: 0.723 ± 0.024	3.028ArgIle: 3.028 ± 0.063	3.025ArgLys: 3.025 ± 0.056	3.815ArgLeu: 3.815 ± 0.057	1.221ArgMet: 1.221 ± 0.034	1.852ArgAsn: 1.852 ± 0.038	1.212ArgPro: 1.212 ± 0.039	1.376ArgGln: 1.376 ± 0.034	1.515ArgArg: 1.515 ± 0.044	2.025ArgSer: 2.025 ± 0.04	1.958ArgThr: 1.958 ± 0.044	2.613ArgVal: 2.613 ± 0.047	0.339ArgTrp: 0.339 ± 0.017	1.346ArgTyr: 1.346 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.785SerAla: 3.785 ± 0.062	0.343SerCys: 0.343 ± 0.019	2.816SerAsp: 2.816 ± 0.048	3.949SerGlu: 3.949 ± 0.059	3.382SerPhe: 3.382 ± 0.058	4.426SerGly: 4.426 ± 0.077	1.172SerHis: 1.172 ± 0.027	5.642SerIle: 5.642 ± 0.071	3.984SerLys: 3.984 ± 0.06	6.048SerLeu: 6.048 ± 0.078	1.758SerMet: 1.758 ± 0.036	2.773SerAsn: 2.773 ± 0.05	2.202SerPro: 2.202 ± 0.043	1.803SerGln: 1.803 ± 0.04	2.149SerArg: 2.149 ± 0.048	3.892SerSer: 3.892 ± 0.071	3.453SerThr: 3.453 ± 0.052	4.32SerVal: 4.32 ± 0.059	0.555SerTrp: 0.555 ± 0.02	2.242SerTyr: 2.242 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.094ThrAla: 4.094 ± 0.069	0.305ThrCys: 0.305 ± 0.016	2.67ThrAsp: 2.67 ± 0.048	3.955ThrGlu: 3.955 ± 0.071	2.801ThrPhe: 2.801 ± 0.05	4.168ThrGly: 4.168 ± 0.061	1.093ThrHis: 1.093 ± 0.03	5.01ThrIle: 5.01 ± 0.066	3.74ThrLys: 3.74 ± 0.052	5.504ThrLeu: 5.504 ± 0.069	1.364ThrMet: 1.364 ± 0.036	2.835ThrAsn: 2.835 ± 0.054	2.385ThrPro: 2.385 ± 0.05	1.495ThrGln: 1.495 ± 0.033	1.769ThrArg: 1.769 ± 0.034	3.616ThrSer: 3.616 ± 0.065	3.372ThrThr: 3.372 ± 0.063	4.249ThrVal: 4.249 ± 0.059	0.537ThrTrp: 0.537 ± 0.02	2.018ThrTyr: 2.018 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	5.244ValAla: 5.244 ± 0.075	0.554ValCys: 0.554 ± 0.021	3.723ValAsp: 3.723 ± 0.06	5.273ValGlu: 5.273 ± 0.077	3.27ValPhe: 3.27 ± 0.06	4.819ValGly: 4.819 ± 0.074	1.336ValHis: 1.336 ± 0.028	5.969ValIle: 5.969 ± 0.082	4.504ValLys: 4.504 ± 0.064	7.014ValLeu: 7.014 ± 0.084	1.908ValMet: 1.908 ± 0.043	3.167ValAsn: 3.167 ± 0.049	2.69ValPro: 2.69 ± 0.046	2.538ValGln: 2.538 ± 0.045	2.564ValArg: 2.564 ± 0.048	4.632ValSer: 4.632 ± 0.061	4.218ValThr: 4.218 ± 0.065	5.369ValVal: 5.369 ± 0.075	0.619ValTrp: 0.619 ± 0.025	2.276ValTyr: 2.276 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.593TrpAla: 0.593 ± 0.022	0.064TrpCys: 0.064 ± 0.007	0.517TrpAsp: 0.517 ± 0.019	0.616TrpGlu: 0.616 ± 0.023	0.521TrpPhe: 0.521 ± 0.021	0.643TrpGly: 0.643 ± 0.026	0.214TrpHis: 0.214 ± 0.016	0.855TrpIle: 0.855 ± 0.031	0.738TrpLys: 0.738 ± 0.026	1.034TrpLeu: 1.034 ± 0.03	0.351TrpMet: 0.351 ± 0.015	0.527TrpAsn: 0.527 ± 0.022	0.23TrpPro: 0.23 ± 0.015	0.307TrpGln: 0.307 ± 0.016	0.402TrpArg: 0.402 ± 0.018	0.623TrpSer: 0.623 ± 0.026	0.552TrpThr: 0.552 ± 0.021	0.603TrpVal: 0.603 ± 0.022	0.125TrpTrp: 0.125 ± 0.011	0.33TrpTyr: 0.33 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.35TyrAla: 2.35 ± 0.047	0.281TyrCys: 0.281 ± 0.019	1.898TyrAsp: 1.898 ± 0.042	2.576TyrGlu: 2.576 ± 0.052	1.827TyrPhe: 1.827 ± 0.039	2.457TyrGly: 2.457 ± 0.043	0.696TyrHis: 0.696 ± 0.022	2.625TyrIle: 2.625 ± 0.05	2.146TyrLys: 2.146 ± 0.038	3.232TyrLeu: 3.232 ± 0.054	0.894TyrMet: 0.894 ± 0.026	1.542TyrAsn: 1.542 ± 0.037	1.322TyrPro: 1.322 ± 0.037	1.169TyrGln: 1.169 ± 0.033	1.424TyrArg: 1.424 ± 0.042	2.055TyrSer: 2.055 ± 0.044	1.887TyrThr: 1.887 ± 0.039	2.208TyrVal: 2.208 ± 0.045	0.376TyrTrp: 0.376 ± 0.018	1.376TyrTyr: 1.376 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4290 proteins (1198288 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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