Pseudomonas phage SCYZ1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Krylovvirus; unclassified Krylovvirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4PZS1|A0A2Z4PZS1_9CAUD Uncharacterized protein OS=Pseudomonas phage SCYZ1 OX=2249467 GN=SCYZ1_38 PE=4 SV=1
MM1 pKa = 6.79QKK3 pKa = 10.34VYY5 pKa = 11.11VFIEE9 pKa = 4.43DD10 pKa = 3.99GGDD13 pKa = 3.32GSAMVHH19 pKa = 5.17FTKK22 pKa = 10.63DD23 pKa = 3.07QTLLSRR29 pKa = 11.84LEE31 pKa = 4.31EE32 pKa = 4.18NDD34 pKa = 3.34PEE36 pKa = 4.26YY37 pKa = 10.89FGLNEE42 pKa = 4.92GYY44 pKa = 10.21SDD46 pKa = 3.79TLIFPADD53 pKa = 3.79FDD55 pKa = 4.73LEE57 pKa = 4.44GAGFRR62 pKa = 11.84FYY64 pKa = 11.47VEE66 pKa = 4.7DD67 pKa = 3.82EE68 pKa = 4.37EE69 pKa = 4.87EE70 pKa = 4.13EE71 pKa = 4.34EE72 pKa = 4.3EE73 pKa = 4.34

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q139|A0A2Z4Q139_9CAUD Uncharacterized protein OS=Pseudomonas phage SCYZ1 OX=2249467 GN=SCYZ1_41 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84NNKK5 pKa = 8.17QQTRR9 pKa = 11.84VKK11 pKa = 10.51ASSAVAAVDD20 pKa = 3.58RR21 pKa = 11.84KK22 pKa = 9.42IAKK25 pKa = 10.05AEE27 pKa = 3.93RR28 pKa = 11.84GLAKK32 pKa = 9.99VDD34 pKa = 4.5RR35 pKa = 11.84YY36 pKa = 10.9CDD38 pKa = 3.3AYY40 pKa = 11.09DD41 pKa = 3.75RR42 pKa = 11.84LQLEE46 pKa = 4.64LRR48 pKa = 11.84QLYY51 pKa = 8.8QKK53 pKa = 10.7RR54 pKa = 11.84SVLFKK59 pKa = 10.63GVRR62 pKa = 11.84NAA64 pKa = 4.01

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

14468

47

1043

233.4

25.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.02 ± 0.577

0.816 ± 0.124

6.248 ± 0.21

6.628 ± 0.33

3.981 ± 0.201

8.398 ± 0.345

1.963 ± 0.19

4.693 ± 0.186

5.82 ± 0.252

8.108 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.18

4.555 ± 0.199

4.14 ± 0.186

4.299 ± 0.326

5.474 ± 0.209

5.675 ± 0.216

5.84 ± 0.254

6.76 ± 0.247

1.576 ± 0.139

3.318 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski