Amino acid dipepetide frequency for Pseudomonas phage SCYZ1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.677AlaAla: 9.677 ± 1.244
0.691AlaCys: 0.691 ± 0.232
5.53AlaAsp: 5.53 ± 0.682
7.051AlaGlu: 7.051 ± 0.873
2.834AlaPhe: 2.834 ± 0.398
7.604AlaGly: 7.604 ± 1.042
1.521AlaHis: 1.521 ± 0.323
4.424AlaIle: 4.424 ± 0.61
5.184AlaLys: 5.184 ± 0.61
7.673AlaLeu: 7.673 ± 0.744
3.18AlaMet: 3.18 ± 0.435
4.009AlaAsn: 4.009 ± 0.491
3.111AlaPro: 3.111 ± 0.567
4.7AlaGln: 4.7 ± 0.839
3.525AlaArg: 3.525 ± 0.571
4.908AlaSer: 4.908 ± 0.549
5.461AlaThr: 5.461 ± 0.615
5.806AlaVal: 5.806 ± 0.829
1.037AlaTrp: 1.037 ± 0.248
2.281AlaTyr: 2.281 ± 0.415
0.0AlaXaa: 0.0 ± 0.0
Cys
0.484CysAla: 0.484 ± 0.19
0.069CysCys: 0.069 ± 0.062
0.415CysAsp: 0.415 ± 0.152
0.553CysGlu: 0.553 ± 0.208
0.276CysPhe: 0.276 ± 0.158
0.76CysGly: 0.76 ± 0.266
0.484CysHis: 0.484 ± 0.153
0.276CysIle: 0.276 ± 0.13
0.484CysLys: 0.484 ± 0.219
0.415CysLeu: 0.415 ± 0.186
0.207CysMet: 0.207 ± 0.124
0.415CysAsn: 0.415 ± 0.16
0.553CysPro: 0.553 ± 0.168
0.346CysGln: 0.346 ± 0.169
0.346CysArg: 0.346 ± 0.13
0.553CysSer: 0.553 ± 0.218
0.553CysThr: 0.553 ± 0.213
0.691CysVal: 0.691 ± 0.236
0.069CysTrp: 0.069 ± 0.062
0.207CysTyr: 0.207 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
5.046AspAla: 5.046 ± 0.781
0.415AspCys: 0.415 ± 0.146
4.078AspAsp: 4.078 ± 0.589
4.286AspGlu: 4.286 ± 0.605
3.111AspPhe: 3.111 ± 0.446
5.668AspGly: 5.668 ± 0.793
1.175AspHis: 1.175 ± 0.261
2.903AspIle: 2.903 ± 0.396
3.249AspLys: 3.249 ± 0.449
5.53AspLeu: 5.53 ± 0.654
1.59AspMet: 1.59 ± 0.285
2.074AspAsn: 2.074 ± 0.299
3.733AspPro: 3.733 ± 0.538
2.35AspGln: 2.35 ± 0.419
3.18AspArg: 3.18 ± 0.436
3.664AspSer: 3.664 ± 0.454
2.972AspThr: 2.972 ± 0.38
4.493AspVal: 4.493 ± 0.544
1.313AspTrp: 1.313 ± 0.225
1.659AspTyr: 1.659 ± 0.442
0.0AspXaa: 0.0 ± 0.0
Glu
6.567GluAla: 6.567 ± 1.003
0.622GluCys: 0.622 ± 0.203
3.525GluAsp: 3.525 ± 0.455
5.875GluGlu: 5.875 ± 0.837
2.696GluPhe: 2.696 ± 0.508
6.014GluGly: 6.014 ± 0.765
1.59GluHis: 1.59 ± 0.396
2.765GluIle: 2.765 ± 0.476
3.802GluLys: 3.802 ± 0.435
6.705GluLeu: 6.705 ± 0.709
1.728GluMet: 1.728 ± 0.265
2.972GluAsn: 2.972 ± 0.423
2.212GluPro: 2.212 ± 0.46
2.35GluGln: 2.35 ± 0.467
3.594GluArg: 3.594 ± 0.533
2.765GluSer: 2.765 ± 0.468
2.143GluThr: 2.143 ± 0.346
4.7GluVal: 4.7 ± 0.56
0.899GluTrp: 0.899 ± 0.288
2.765GluTyr: 2.765 ± 0.395
0.0GluXaa: 0.0 ± 0.0
Phe
2.834PheAla: 2.834 ± 0.425
0.346PheCys: 0.346 ± 0.174
2.972PheAsp: 2.972 ± 0.325
2.558PheGlu: 2.558 ± 0.502
1.382PhePhe: 1.382 ± 0.303
4.216PheGly: 4.216 ± 0.448
1.106PheHis: 1.106 ± 0.242
2.419PheIle: 2.419 ± 0.328
2.281PheLys: 2.281 ± 0.327
2.903PheLeu: 2.903 ± 0.374
1.106PheMet: 1.106 ± 0.301
1.935PheAsn: 1.935 ± 0.358
1.382PhePro: 1.382 ± 0.366
2.005PheGln: 2.005 ± 0.375
1.452PheArg: 1.452 ± 0.305
2.627PheSer: 2.627 ± 0.408
2.074PheThr: 2.074 ± 0.359
2.35PheVal: 2.35 ± 0.381
0.553PheTrp: 0.553 ± 0.172
1.313PheTyr: 1.313 ± 0.349
0.0PheXaa: 0.0 ± 0.0
Gly
8.156GlyAla: 8.156 ± 1.027
0.968GlyCys: 0.968 ± 0.292
4.631GlyAsp: 4.631 ± 0.582
3.94GlyGlu: 3.94 ± 0.497
2.903GlyPhe: 2.903 ± 0.478
7.12GlyGly: 7.12 ± 1.126
1.521GlyHis: 1.521 ± 0.318
4.078GlyIle: 4.078 ± 0.414
6.498GlyLys: 6.498 ± 0.829
6.498GlyLeu: 6.498 ± 0.669
2.419GlyMet: 2.419 ± 0.427
3.733GlyAsn: 3.733 ± 0.493
1.935GlyPro: 1.935 ± 0.363
3.387GlyGln: 3.387 ± 0.693
4.839GlyArg: 4.839 ± 0.485
5.737GlySer: 5.737 ± 0.552
5.184GlyThr: 5.184 ± 0.732
5.53GlyVal: 5.53 ± 0.668
1.382GlyTrp: 1.382 ± 0.338
3.525GlyTyr: 3.525 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
1.313HisAla: 1.313 ± 0.349
0.069HisCys: 0.069 ± 0.075
0.899HisAsp: 0.899 ± 0.256
1.313HisGlu: 1.313 ± 0.335
0.899HisPhe: 0.899 ± 0.211
1.728HisGly: 1.728 ± 0.343
0.346HisHis: 0.346 ± 0.187
1.382HisIle: 1.382 ± 0.353
0.968HisLys: 0.968 ± 0.274
2.281HisLeu: 2.281 ± 0.323
0.346HisMet: 0.346 ± 0.144
0.622HisAsn: 0.622 ± 0.191
0.76HisPro: 0.76 ± 0.2
0.76HisGln: 0.76 ± 0.2
1.244HisArg: 1.244 ± 0.289
0.899HisSer: 0.899 ± 0.228
1.175HisThr: 1.175 ± 0.333
1.244HisVal: 1.244 ± 0.264
0.76HisTrp: 0.76 ± 0.276
0.622HisTyr: 0.622 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
4.147IleAla: 4.147 ± 0.538
0.346IleCys: 0.346 ± 0.169
2.972IleAsp: 2.972 ± 0.474
2.765IleGlu: 2.765 ± 0.399
1.244IlePhe: 1.244 ± 0.283
3.94IleGly: 3.94 ± 0.579
0.691IleHis: 0.691 ± 0.218
1.866IleIle: 1.866 ± 0.407
3.456IleLys: 3.456 ± 0.521
3.249IleLeu: 3.249 ± 0.426
1.659IleMet: 1.659 ± 0.407
2.972IleAsn: 2.972 ± 0.495
2.143IlePro: 2.143 ± 0.388
2.074IleGln: 2.074 ± 0.406
2.419IleArg: 2.419 ± 0.368
2.765IleSer: 2.765 ± 0.475
2.765IleThr: 2.765 ± 0.478
3.041IleVal: 3.041 ± 0.453
0.829IleTrp: 0.829 ± 0.224
1.521IleTyr: 1.521 ± 0.297
0.0IleXaa: 0.0 ± 0.0
Lys
5.53LysAla: 5.53 ± 0.634
0.484LysCys: 0.484 ± 0.221
3.802LysAsp: 3.802 ± 0.477
4.078LysGlu: 4.078 ± 0.436
2.834LysPhe: 2.834 ± 0.36
4.7LysGly: 4.7 ± 0.596
1.382LysHis: 1.382 ± 0.35
2.972LysIle: 2.972 ± 0.482
2.074LysLys: 2.074 ± 0.35
4.977LysLeu: 4.977 ± 0.67
1.382LysMet: 1.382 ± 0.3
1.866LysAsn: 1.866 ± 0.425
2.765LysPro: 2.765 ± 0.564
2.281LysGln: 2.281 ± 0.412
2.972LysArg: 2.972 ± 0.557
3.249LysSer: 3.249 ± 0.567
3.664LysThr: 3.664 ± 0.556
4.631LysVal: 4.631 ± 0.53
0.622LysTrp: 0.622 ± 0.201
1.935LysTyr: 1.935 ± 0.262
0.0LysXaa: 0.0 ± 0.0
Leu
6.567LeuAla: 6.567 ± 0.722
0.691LeuCys: 0.691 ± 0.212
5.046LeuAsp: 5.046 ± 0.553
5.737LeuGlu: 5.737 ± 0.648
3.18LeuPhe: 3.18 ± 0.423
6.428LeuGly: 6.428 ± 0.757
1.452LeuHis: 1.452 ± 0.269
2.834LeuIle: 2.834 ± 0.372
5.945LeuLys: 5.945 ± 0.635
6.083LeuLeu: 6.083 ± 0.554
1.935LeuMet: 1.935 ± 0.515
3.664LeuAsn: 3.664 ± 0.39
3.664LeuPro: 3.664 ± 0.545
3.111LeuGln: 3.111 ± 0.482
5.875LeuArg: 5.875 ± 0.685
5.737LeuSer: 5.737 ± 0.58
5.046LeuThr: 5.046 ± 0.677
4.7LeuVal: 4.7 ± 0.7
1.382LeuTrp: 1.382 ± 0.304
2.005LeuTyr: 2.005 ± 0.366
0.0LeuXaa: 0.0 ± 0.0
Met
3.18MetAla: 3.18 ± 0.681
0.346MetCys: 0.346 ± 0.175
1.935MetAsp: 1.935 ± 0.312
1.59MetGlu: 1.59 ± 0.323
0.829MetPhe: 0.829 ± 0.237
1.866MetGly: 1.866 ± 0.457
0.622MetHis: 0.622 ± 0.195
1.175MetIle: 1.175 ± 0.32
1.452MetLys: 1.452 ± 0.346
2.558MetLeu: 2.558 ± 0.422
0.553MetMet: 0.553 ± 0.221
1.037MetAsn: 1.037 ± 0.244
0.899MetPro: 0.899 ± 0.222
1.037MetGln: 1.037 ± 0.289
1.175MetArg: 1.175 ± 0.27
2.627MetSer: 2.627 ± 0.493
1.452MetThr: 1.452 ± 0.279
1.313MetVal: 1.313 ± 0.315
0.346MetTrp: 0.346 ± 0.126
0.899MetTyr: 0.899 ± 0.233
0.0MetXaa: 0.0 ± 0.0
Asn
3.802AsnAla: 3.802 ± 0.535
0.346AsnCys: 0.346 ± 0.136
2.627AsnAsp: 2.627 ± 0.376
2.074AsnGlu: 2.074 ± 0.351
2.074AsnPhe: 2.074 ± 0.254
3.664AsnGly: 3.664 ± 0.58
0.76AsnHis: 0.76 ± 0.252
2.627AsnIle: 2.627 ± 0.4
2.212AsnLys: 2.212 ± 0.406
3.18AsnLeu: 3.18 ± 0.359
1.382AsnMet: 1.382 ± 0.359
2.074AsnAsn: 2.074 ± 0.409
2.212AsnPro: 2.212 ± 0.351
2.212AsnGln: 2.212 ± 0.34
3.111AsnArg: 3.111 ± 0.447
2.765AsnSer: 2.765 ± 0.397
3.111AsnThr: 3.111 ± 0.39
3.525AsnVal: 3.525 ± 0.531
0.76AsnTrp: 0.76 ± 0.196
1.037AsnTyr: 1.037 ± 0.247
0.0AsnXaa: 0.0 ± 0.0
Pro
3.94ProAla: 3.94 ± 0.519
0.346ProCys: 0.346 ± 0.164
3.525ProAsp: 3.525 ± 0.517
3.318ProGlu: 3.318 ± 0.574
2.35ProPhe: 2.35 ± 0.354
3.18ProGly: 3.18 ± 0.523
0.691ProHis: 0.691 ± 0.243
1.935ProIle: 1.935 ± 0.403
2.143ProLys: 2.143 ± 0.359
2.627ProLeu: 2.627 ± 0.304
1.037ProMet: 1.037 ± 0.26
1.866ProAsn: 1.866 ± 0.326
1.106ProPro: 1.106 ± 0.267
2.281ProGln: 2.281 ± 0.495
1.175ProArg: 1.175 ± 0.336
2.281ProSer: 2.281 ± 0.413
2.696ProThr: 2.696 ± 0.34
2.834ProVal: 2.834 ± 0.467
0.622ProTrp: 0.622 ± 0.179
1.452ProTyr: 1.452 ± 0.376
0.0ProXaa: 0.0 ± 0.0
Gln
4.562GlnAla: 4.562 ± 0.692
0.207GlnCys: 0.207 ± 0.115
2.765GlnAsp: 2.765 ± 0.433
3.525GlnGlu: 3.525 ± 0.71
1.521GlnPhe: 1.521 ± 0.292
3.249GlnGly: 3.249 ± 0.497
0.484GlnHis: 0.484 ± 0.186
1.728GlnIle: 1.728 ± 0.338
1.866GlnLys: 1.866 ± 0.324
4.7GlnLeu: 4.7 ± 0.57
0.829GlnMet: 0.829 ± 0.196
1.728GlnAsn: 1.728 ± 0.331
1.382GlnPro: 1.382 ± 0.403
2.35GlnGln: 2.35 ± 0.643
3.249GlnArg: 3.249 ± 0.472
2.074GlnSer: 2.074 ± 0.42
2.35GlnThr: 2.35 ± 0.377
2.627GlnVal: 2.627 ± 0.423
0.622GlnTrp: 0.622 ± 0.224
1.175GlnTyr: 1.175 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
4.562ArgAla: 4.562 ± 0.502
0.346ArgCys: 0.346 ± 0.118
3.249ArgAsp: 3.249 ± 0.475
2.627ArgGlu: 2.627 ± 0.465
1.797ArgPhe: 1.797 ± 0.368
3.802ArgGly: 3.802 ± 0.539
1.106ArgHis: 1.106 ± 0.345
3.318ArgIle: 3.318 ± 0.489
4.078ArgLys: 4.078 ± 0.478
4.7ArgLeu: 4.7 ± 0.676
1.521ArgMet: 1.521 ± 0.342
3.318ArgAsn: 3.318 ± 0.541
2.212ArgPro: 2.212 ± 0.386
2.281ArgGln: 2.281 ± 0.344
3.111ArgArg: 3.111 ± 0.392
2.972ArgSer: 2.972 ± 0.442
3.387ArgThr: 3.387 ± 0.454
3.525ArgVal: 3.525 ± 0.46
0.76ArgTrp: 0.76 ± 0.239
2.074ArgTyr: 2.074 ± 0.296
0.0ArgXaa: 0.0 ± 0.0
Ser
5.046SerAla: 5.046 ± 0.653
0.484SerCys: 0.484 ± 0.172
3.387SerAsp: 3.387 ± 0.508
3.733SerGlu: 3.733 ± 0.427
1.797SerPhe: 1.797 ± 0.307
5.392SerGly: 5.392 ± 0.696
1.313SerHis: 1.313 ± 0.303
2.972SerIle: 2.972 ± 0.426
2.903SerLys: 2.903 ± 0.434
3.94SerLeu: 3.94 ± 0.455
1.935SerMet: 1.935 ± 0.274
2.143SerAsn: 2.143 ± 0.532
2.419SerPro: 2.419 ± 0.42
2.972SerGln: 2.972 ± 0.399
3.318SerArg: 3.318 ± 0.429
2.558SerSer: 2.558 ± 0.348
3.733SerThr: 3.733 ± 0.489
3.525SerVal: 3.525 ± 0.456
1.175SerTrp: 1.175 ± 0.306
2.005SerTyr: 2.005 ± 0.426
0.0SerXaa: 0.0 ± 0.0
Thr
5.322ThrAla: 5.322 ± 0.66
0.484ThrCys: 0.484 ± 0.175
2.765ThrAsp: 2.765 ± 0.399
3.456ThrGlu: 3.456 ± 0.386
2.696ThrPhe: 2.696 ± 0.427
5.184ThrGly: 5.184 ± 0.571
1.037ThrHis: 1.037 ± 0.277
2.074ThrIle: 2.074 ± 0.389
3.387ThrLys: 3.387 ± 0.463
5.322ThrLeu: 5.322 ± 0.527
1.175ThrMet: 1.175 ± 0.26
2.627ThrAsn: 2.627 ± 0.419
3.664ThrPro: 3.664 ± 0.518
2.419ThrGln: 2.419 ± 0.401
2.903ThrArg: 2.903 ± 0.437
2.558ThrSer: 2.558 ± 0.402
3.733ThrThr: 3.733 ± 0.403
4.908ThrVal: 4.908 ± 0.597
0.968ThrTrp: 0.968 ± 0.316
1.728ThrTyr: 1.728 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
5.599ValAla: 5.599 ± 0.736
0.415ValCys: 0.415 ± 0.155
3.94ValAsp: 3.94 ± 0.604
4.769ValGlu: 4.769 ± 0.601
3.18ValPhe: 3.18 ± 0.394
5.599ValGly: 5.599 ± 0.678
1.313ValHis: 1.313 ± 0.293
2.696ValIle: 2.696 ± 0.43
3.456ValLys: 3.456 ± 0.476
4.286ValLeu: 4.286 ± 0.706
1.797ValMet: 1.797 ± 0.389
3.871ValAsn: 3.871 ± 0.456
3.318ValPro: 3.318 ± 0.366
2.143ValGln: 2.143 ± 0.288
4.424ValArg: 4.424 ± 0.533
3.18ValSer: 3.18 ± 0.456
4.424ValThr: 4.424 ± 0.641
4.977ValVal: 4.977 ± 0.561
0.899ValTrp: 0.899 ± 0.237
3.318ValTyr: 3.318 ± 0.489
0.0ValXaa: 0.0 ± 0.0
Trp
1.382TrpAla: 1.382 ± 0.324
0.138TrpCys: 0.138 ± 0.098
0.968TrpAsp: 0.968 ± 0.196
1.037TrpGlu: 1.037 ± 0.254
0.829TrpPhe: 0.829 ± 0.234
1.59TrpGly: 1.59 ± 0.319
0.138TrpHis: 0.138 ± 0.087
0.622TrpIle: 0.622 ± 0.216
0.829TrpLys: 0.829 ± 0.253
1.313TrpLeu: 1.313 ± 0.337
0.484TrpMet: 0.484 ± 0.148
0.829TrpAsn: 0.829 ± 0.259
0.415TrpPro: 0.415 ± 0.189
0.415TrpGln: 0.415 ± 0.132
1.106TrpArg: 1.106 ± 0.278
1.313TrpSer: 1.313 ± 0.336
0.622TrpThr: 0.622 ± 0.224
0.968TrpVal: 0.968 ± 0.241
0.276TrpTrp: 0.276 ± 0.16
0.484TrpTyr: 0.484 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.488TyrAla: 2.488 ± 0.39
0.346TyrCys: 0.346 ± 0.172
3.456TyrAsp: 3.456 ± 0.428
2.005TyrGlu: 2.005 ± 0.389
1.382TyrPhe: 1.382 ± 0.331
2.281TyrGly: 2.281 ± 0.347
0.899TyrHis: 0.899 ± 0.261
1.866TyrIle: 1.866 ± 0.417
1.935TyrLys: 1.935 ± 0.354
2.143TyrLeu: 2.143 ± 0.357
0.553TyrMet: 0.553 ± 0.21
1.797TyrAsn: 1.797 ± 0.359
1.521TyrPro: 1.521 ± 0.317
1.521TyrGln: 1.521 ± 0.26
1.728TyrArg: 1.728 ± 0.366
1.521TyrSer: 1.521 ± 0.343
1.866TyrThr: 1.866 ± 0.301
2.212TyrVal: 2.212 ± 0.401
0.484TyrTrp: 0.484 ± 0.165
1.175TyrTyr: 1.175 ± 0.249
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (14468 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski