Candidatus Vidania fulgoroideae
Average proteome isoelectric point is 9.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346E0L8|A0A346E0L8_9PROT tRNA-specific 2-thiouridylase MnmA OS=Candidatus Vidania fulgoroideae OX=881286 GN=C9I84_136 PE=4 SV=1
MM1 pKa = 6.82 EE2 pKa = 4.81 TSFGLYY8 pKa = 9.7 IMNNIIGIDD17 pKa = 3.42 LGTTNSCVSIMEE29 pKa = 4.39 GNTAKK34 pKa = 10.74 VIEE37 pKa = 4.28 NSEE40 pKa = 4.47 GYY42 pKa = 7.92 RR43 pKa = 11.84 TTPSVVAYY51 pKa = 10.55 CNDD54 pKa = 3.91 TILVGEE60 pKa = 4.25 SAKK63 pKa = 10.53 RR64 pKa = 11.84 QSLTNPKK71 pKa = 8.55 NTIFAVKK78 pKa = 10.02 RR79 pKa = 11.84 LIGRR83 pKa = 11.84 KK84 pKa = 8.15 FTDD87 pKa = 3.42 PEE89 pKa = 3.79 MLEE92 pKa = 4.28 NIKK95 pKa = 9.68 TSPYY99 pKa = 10.21 KK100 pKa = 10.03 IVKK103 pKa = 9.91 AEE105 pKa = 4.4 NGDD108 pKa = 3.13 AWVEE112 pKa = 3.83 IEE114 pKa = 4.28 NGKK117 pKa = 10.25 KK118 pKa = 9.42 IAPQQVSSEE127 pKa = 4.06 ILKK130 pKa = 10.61 KK131 pKa = 9.62 MKK133 pKa = 9.1 KK134 pKa = 7.37 TAEE137 pKa = 4.12 SYY139 pKa = 10.92 LGFEE143 pKa = 4.26 IKK145 pKa = 10.25 EE146 pKa = 4.06 AVITVPAYY154 pKa = 10.64 FNDD157 pKa = 3.76 SQRR160 pKa = 11.84 QATKK164 pKa = 10.34 DD165 pKa = 2.98 AGMIAGLKK173 pKa = 9.49 INRR176 pKa = 11.84 IVNEE180 pKa = 3.8 PTAAALAFGLDD191 pKa = 3.5 KK192 pKa = 11.27 NIEE195 pKa = 4.09 KK196 pKa = 10.2 EE197 pKa = 4.16 KK198 pKa = 10.86 KK199 pKa = 9.32 IVVYY203 pKa = 10.53 DD204 pKa = 3.88 LGGGTFDD211 pKa = 3.48 ISVIEE216 pKa = 4.31 ISMIDD221 pKa = 3.46 NEE223 pKa = 4.42 KK224 pKa = 10.37 QFEE227 pKa = 4.31 VLSTNGDD234 pKa = 3.7 TLLGGEE240 pKa = 4.45 DD241 pKa = 4.16 FDD243 pKa = 5.27 RR244 pKa = 11.84 KK245 pKa = 9.96 IIKK248 pKa = 9.84 FLIDD252 pKa = 3.39 KK253 pKa = 9.17 YY254 pKa = 11.32 LSIEE258 pKa = 4.57 GIDD261 pKa = 3.7 LEE263 pKa = 4.48 KK264 pKa = 10.92 DD265 pKa = 2.72 ILALQRR271 pKa = 11.84 LKK273 pKa = 10.74 EE274 pKa = 4.03 ASEE277 pKa = 4.1 KK278 pKa = 10.74 AKK280 pKa = 10.89 KK281 pKa = 9.69 EE282 pKa = 3.8 LSNLEE287 pKa = 3.95 STEE290 pKa = 3.65 INLPYY295 pKa = 10.24 ISINSSGPKK304 pKa = 9.42 HH305 pKa = 5.85 MKK307 pKa = 9.96 ILLTRR312 pKa = 11.84 AKK314 pKa = 10.74 YY315 pKa = 10.07 EE316 pKa = 3.83 FLIEE320 pKa = 4.41 DD321 pKa = 4.26 LVKK324 pKa = 10.03 KK325 pKa = 8.57 TLIPCEE331 pKa = 3.96 TALKK335 pKa = 10.49 DD336 pKa = 3.61 SLINISDD343 pKa = 3.45 IDD345 pKa = 4.57 DD346 pKa = 4.6 IILVGGMTRR355 pKa = 11.84 TPIIQKK361 pKa = 9.68 RR362 pKa = 11.84 VRR364 pKa = 11.84 EE365 pKa = 4.04 FFKK368 pKa = 11.01 RR369 pKa = 11.84 EE370 pKa = 3.63 PKK372 pKa = 10.62 KK373 pKa = 10.81 NINPDD378 pKa = 3.03 EE379 pKa = 4.18 AVAIGASIQAQILSGDD395 pKa = 3.72 RR396 pKa = 11.84 KK397 pKa = 10.66 DD398 pKa = 4.46 VLLLDD403 pKa = 3.87 VTPLSLGIEE412 pKa = 4.29 TLGGVMTKK420 pKa = 9.89 MIVKK424 pKa = 8.49 NTTIPTKK431 pKa = 10.22 CSQVFSTAEE440 pKa = 3.82 EE441 pKa = 4.14 NQNSVTIKK449 pKa = 9.68 VYY451 pKa = 9.95 QGEE454 pKa = 4.15 RR455 pKa = 11.84 SVAKK459 pKa = 9.57 TNKK462 pKa = 10.23 LLGEE466 pKa = 4.31 FNLEE470 pKa = 4.23 EE471 pKa = 4.48 IEE473 pKa = 4.05 PAPRR477 pKa = 11.84 GVPQIEE483 pKa = 4.25 VMFDD487 pKa = 2.82 IDD489 pKa = 4.69 SNGILNVSARR499 pKa = 11.84 NKK501 pKa = 8.89 VTGKK505 pKa = 9.64 EE506 pKa = 3.77 NKK508 pKa = 9.46 IVIKK512 pKa = 10.67 VNSGLTEE519 pKa = 3.9 EE520 pKa = 5.2 EE521 pKa = 3.92 IEE523 pKa = 4.75 NMINEE528 pKa = 4.17 AKK530 pKa = 10.6 KK531 pKa = 10.65 NEE533 pKa = 4.14 EE534 pKa = 3.66 KK535 pKa = 10.99 DD536 pKa = 3.62 KK537 pKa = 10.64 ITLEE541 pKa = 4.28 TINLKK546 pKa = 10.62 NKK548 pKa = 9.91 IEE550 pKa = 3.86 NLINQSEE557 pKa = 4.39 KK558 pKa = 10.36 IIKK561 pKa = 9.86 EE562 pKa = 3.79 NLNFIEE568 pKa = 4.37 KK569 pKa = 10.12 EE570 pKa = 4.01 NYY572 pKa = 10.18 LEE574 pKa = 5.62 AEE576 pKa = 4.46 TILNEE581 pKa = 4.37 NKK583 pKa = 9.78 ISYY586 pKa = 9.96 LNKK589 pKa = 10.13 KK590 pKa = 10.42 KK591 pKa = 10.58 EE592 pKa = 3.9 DD593 pKa = 3.62 LEE595 pKa = 4.56 LNIINIEE602 pKa = 4.03 NVMKK606 pKa = 10.64 KK607 pKa = 10.7 VNDD610 pKa = 4.54 DD611 pKa = 2.99 ILRR614 pKa = 11.84 NKK616 pKa = 9.4 KK617 pKa = 9.92
Molecular weight: 69.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.351
IPC2_protein 5.448
IPC_protein 5.359
Toseland 5.232
ProMoST 5.436
Dawson 5.283
Bjellqvist 5.423
Wikipedia 5.105
Rodwell 5.207
Grimsley 5.143
Solomon 5.283
Lehninger 5.232
Nozaki 5.385
DTASelect 5.486
Thurlkill 5.219
EMBOSS 5.13
Sillero 5.474
Patrickios 4.647
IPC_peptide 5.296
IPC2_peptide 5.461
IPC2.peptide.svr19 5.627
Protein with the highest isoelectric point:
>tr|A0A346E0I9|A0A346E0I9_9PROT Uncharacterized protein OS=Candidatus Vidania fulgoroideae OX=881286 GN=C9I84_102 PE=4 SV=1
MM1 pKa = 7.21 SRR3 pKa = 11.84 VKK5 pKa = 10.61 RR6 pKa = 11.84 GVFSKK11 pKa = 10.51 KK12 pKa = 7.65 KK13 pKa = 8.23 HH14 pKa = 5.34 KK15 pKa = 10.55 KK16 pKa = 8.88 IIKK19 pKa = 8.27 RR20 pKa = 11.84 CKK22 pKa = 9.66 GFTGRR27 pKa = 11.84 RR28 pKa = 11.84 KK29 pKa = 9.78 NVFRR33 pKa = 11.84 ISKK36 pKa = 8.49 QAYY39 pKa = 8.26 IKK41 pKa = 10.37 SLQYY45 pKa = 10.12 FYY47 pKa = 11.35 RR48 pKa = 11.84 DD49 pKa = 3.18 TRR51 pKa = 11.84 NKK53 pKa = 10.1 KK54 pKa = 9.68 RR55 pKa = 11.84 DD56 pKa = 3.57 FRR58 pKa = 11.84 KK59 pKa = 9.99 LWIININFYY68 pKa = 10.34 LRR70 pKa = 11.84 KK71 pKa = 10.15 YY72 pKa = 8.44 NIKK75 pKa = 8.76 YY76 pKa = 10.45 SRR78 pKa = 11.84 FIKK81 pKa = 10.22 YY82 pKa = 9.78 IKK84 pKa = 9.71 EE85 pKa = 3.65 LNININRR92 pKa = 11.84 KK93 pKa = 8.24 SLYY96 pKa = 9.43 YY97 pKa = 10.31 LCKK100 pKa = 9.62 EE101 pKa = 4.06 YY102 pKa = 11.36 NMKK105 pKa = 10.61 KK106 pKa = 10.3 LIIYY110 pKa = 10.06 INEE113 pKa = 3.94 KK114 pKa = 10.59 KK115 pKa = 10.73 YY116 pKa = 10.83 LQNN119 pKa = 3.4
Molecular weight: 15.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.275
IPC2_protein 10.233
IPC_protein 10.672
Toseland 10.818
ProMoST 10.423
Dawson 10.935
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 11.447
Grimsley 10.979
Solomon 10.979
Lehninger 10.965
Nozaki 10.789
DTASelect 10.584
Thurlkill 10.818
EMBOSS 11.213
Sillero 10.847
Patrickios 11.155
IPC_peptide 10.994
IPC2_peptide 9.37
IPC2.peptide.svr19 8.099
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
154
0
154
42964
37
1292
279.0
33.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.543 ± 0.133
1.576 ± 0.055
2.795 ± 0.092
4.19 ± 0.178
7.595 ± 0.277
4.029 ± 0.184
0.968 ± 0.059
14.74 ± 0.187
19.156 ± 0.37
7.8 ± 0.126
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.536 ± 0.063
9.943 ± 0.189
1.769 ± 0.087
1.213 ± 0.063
2.9 ± 0.103
6.28 ± 0.156
3.182 ± 0.091
3.475 ± 0.125
0.314 ± 0.05
4.976 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here