Saccharothrix australiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharothrix

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6519 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495VUQ9|A0A495VUQ9_9PSEU 8-oxo-dGTP diphosphatase OS=Saccharothrix australiensis OX=2072 GN=C8E97_1132 PE=4 SV=1
MM1 pKa = 7.48LKK3 pKa = 10.27KK4 pKa = 10.58ASTVAGLVTGLMMIGAPAFAVAGEE28 pKa = 4.13PHH30 pKa = 5.89EE31 pKa = 5.19HH32 pKa = 5.52GHH34 pKa = 6.14FADD37 pKa = 3.66GHH39 pKa = 5.93HH40 pKa = 6.48FADD43 pKa = 3.79GHH45 pKa = 6.1HH46 pKa = 6.42FAANDD51 pKa = 3.47VDD53 pKa = 3.62VDD55 pKa = 4.09SYY57 pKa = 11.87DD58 pKa = 3.76GEE60 pKa = 4.39EE61 pKa = 4.49EE62 pKa = 4.6GDD64 pKa = 3.69DD65 pKa = 4.06GQIGLINFNDD75 pKa = 4.08DD76 pKa = 3.4SDD78 pKa = 4.08LASNINLCQIEE89 pKa = 4.38INVIGIPVLSHH100 pKa = 6.46NDD102 pKa = 3.51GYY104 pKa = 11.8GNGGGQSCVNTDD116 pKa = 4.56NDD118 pKa = 5.17DD119 pKa = 4.03NDD121 pKa = 4.42NNDD124 pKa = 4.27DD125 pKa = 3.98NDD127 pKa = 3.87SLEE130 pKa = 4.38YY131 pKa = 10.8EE132 pKa = 4.49DD133 pKa = 5.22

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495VS79|A0A495VS79_9PSEU DNA-binding transcriptional MerR regulator OS=Saccharothrix australiensis OX=2072 GN=C8E97_0254 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.77GRR42 pKa = 11.84KK43 pKa = 7.91QLSAA47 pKa = 3.9

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6519

0

6519

2270699

29

8263

348.3

37.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.088 ± 0.055

0.773 ± 0.01

6.305 ± 0.025

5.457 ± 0.024

2.75 ± 0.016

9.514 ± 0.038

2.365 ± 0.018

2.542 ± 0.022

1.671 ± 0.02

10.554 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.413 ± 0.012

1.609 ± 0.016

6.218 ± 0.031

2.346 ± 0.017

8.807 ± 0.036

4.691 ± 0.022

5.715 ± 0.034

9.858 ± 0.033

1.523 ± 0.013

1.803 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski