Sulfolobus sp. F3
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1702 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A550D2Q0|A0A550D2Q0_9CREN Uncharacterized protein (Fragment) OS=Sulfolobus sp. F3 OX=2200886 GN=DJ522_05725 PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 4.13 NAVTEE7 pKa = 4.47 FVLVVVTILIGLVLFSLVSSYY28 pKa = 9.03 STYY31 pKa = 10.32 QVSNYY36 pKa = 10.19 AVLSEE41 pKa = 4.17 AQQIAYY47 pKa = 9.38 NLKK50 pKa = 9.28 IDD52 pKa = 3.97 IVTLEE57 pKa = 4.59 DD58 pKa = 4.74 GYY60 pKa = 9.47 TLIVPYY66 pKa = 10.23 SYY68 pKa = 11.32 SSYY71 pKa = 11.17 NGSLYY76 pKa = 9.95 LTVFQAPAYY85 pKa = 9.74 LINSSNLLNPTMPGISYY102 pKa = 7.71 VTIYY106 pKa = 11.07 NSTSGNQISFIQLDD120 pKa = 3.53 GRR122 pKa = 11.84 IYY124 pKa = 10.83 SLSNQQLPSPLSVII138 pKa = 4.24
Molecular weight: 15.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.923
IPC2_protein 3.923
IPC_protein 3.706
Toseland 3.541
ProMoST 3.923
Dawson 3.719
Bjellqvist 3.897
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.465
Solomon 3.668
Lehninger 3.63
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.846
Patrickios 0.006
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A550D533|A0A550D533_9CREN Uncharacterized protein (Fragment) OS=Sulfolobus sp. F3 OX=2200886 GN=DJ522_01325 PE=4 SV=1
MM1 pKa = 7.56 SFNKK5 pKa = 9.82 FLSLFSLNRR14 pKa = 11.84 SKK16 pKa = 9.92 TKK18 pKa = 10.06 RR19 pKa = 11.84 KK20 pKa = 9.33 PFFTVRR26 pKa = 11.84 DD27 pKa = 3.5 KK28 pKa = 11.76 AEE30 pKa = 3.71 QRR32 pKa = 11.84 GVKK35 pKa = 10.47 GNLQTVAQISFPLFLSPLLVINRR58 pKa = 11.84 KK59 pKa = 8.02 EE60 pKa = 4.39 FILLCNPRR68 pKa = 11.84 PKK70 pKa = 10.58 GRR72 pKa = 11.84 EE73 pKa = 3.61 FHH75 pKa = 6.91 HH76 pKa = 6.81 LCKK79 pKa = 9.85 PWVGFEE85 pKa = 4.39 PGPLPYY91 pKa = 10.51 QIDD94 pKa = 3.85 RR95 pKa = 11.84 QNINYY100 pKa = 8.79 IRR102 pKa = 11.84 EE103 pKa = 4.13 LMSEE107 pKa = 4.45 DD108 pKa = 2.73 IHH110 pKa = 6.61 RR111 pKa = 11.84 HH112 pKa = 3.71 SRR114 pKa = 11.84 EE115 pKa = 3.56 TRR117 pKa = 11.84 KK118 pKa = 10.06 RR119 pKa = 3.4
Molecular weight: 14.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.663
IPC_protein 10.233
Toseland 10.862
ProMoST 10.701
Dawson 10.921
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.242
Grimsley 10.95
Solomon 11.052
Lehninger 11.023
Nozaki 10.847
DTASelect 10.584
Thurlkill 10.847
EMBOSS 11.257
Sillero 10.862
Patrickios 10.979
IPC_peptide 11.052
IPC2_peptide 9.472
IPC2.peptide.svr19 8.754
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1702
0
1702
403996
31
1050
237.4
26.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.584 ± 0.06
0.626 ± 0.02
4.866 ± 0.047
6.762 ± 0.074
4.41 ± 0.049
6.639 ± 0.052
1.307 ± 0.022
9.8 ± 0.074
7.544 ± 0.071
10.208 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.213 ± 0.024
5.028 ± 0.053
3.941 ± 0.045
2.025 ± 0.034
4.365 ± 0.049
6.835 ± 0.048
4.549 ± 0.052
7.484 ± 0.056
1.046 ± 0.022
4.769 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here