Amino acid dipepetide frequency for Sulfolobus sp. F3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.408AlaAla: 2.408 ± 0.102
0.381AlaCys: 0.381 ± 0.028
2.369AlaAsp: 2.369 ± 0.077
3.52AlaGlu: 3.52 ± 0.104
2.824AlaPhe: 2.824 ± 0.091
3.936AlaGly: 3.936 ± 0.096
0.777AlaHis: 0.777 ± 0.043
6.025AlaIle: 6.025 ± 0.126
4.131AlaLys: 4.131 ± 0.115
6.48AlaLeu: 6.48 ± 0.15
1.307AlaMet: 1.307 ± 0.053
2.413AlaAsn: 2.413 ± 0.081
1.542AlaPro: 1.542 ± 0.067
1.082AlaGln: 1.082 ± 0.052
2.443AlaArg: 2.443 ± 0.087
4.168AlaSer: 4.168 ± 0.1
2.554AlaThr: 2.554 ± 0.081
3.985AlaVal: 3.985 ± 0.128
0.517AlaTrp: 0.517 ± 0.037
2.975AlaTyr: 2.975 ± 0.1
0.0AlaXaa: 0.0 ± 0.0
Cys
0.28CysAla: 0.28 ± 0.027
0.077CysCys: 0.077 ± 0.014
0.297CysAsp: 0.297 ± 0.023
0.473CysGlu: 0.473 ± 0.042
0.248CysPhe: 0.248 ± 0.028
0.559CysGly: 0.559 ± 0.045
0.109CysHis: 0.109 ± 0.017
0.455CysIle: 0.455 ± 0.03
0.616CysLys: 0.616 ± 0.042
0.413CysLeu: 0.413 ± 0.032
0.131CysMet: 0.131 ± 0.019
0.369CysAsn: 0.369 ± 0.034
0.493CysPro: 0.493 ± 0.042
0.099CysGln: 0.099 ± 0.014
0.267CysArg: 0.267 ± 0.024
0.347CysSer: 0.347 ± 0.03
0.29CysThr: 0.29 ± 0.029
0.399CysVal: 0.399 ± 0.036
0.101CysTrp: 0.101 ± 0.015
0.233CysTyr: 0.233 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
2.406AspAla: 2.406 ± 0.08
0.29AspCys: 0.29 ± 0.025
2.418AspAsp: 2.418 ± 0.081
4.183AspGlu: 4.183 ± 0.105
2.334AspPhe: 2.334 ± 0.074
2.733AspGly: 2.733 ± 0.089
0.567AspHis: 0.567 ± 0.038
4.899AspIle: 4.899 ± 0.108
4.017AspLys: 4.017 ± 0.106
5.064AspLeu: 5.064 ± 0.103
1.101AspMet: 1.101 ± 0.049
2.29AspAsn: 2.29 ± 0.092
2.334AspPro: 2.334 ± 0.087
0.866AspGln: 0.866 ± 0.047
1.886AspArg: 1.886 ± 0.061
2.715AspSer: 2.715 ± 0.081
1.594AspThr: 1.594 ± 0.064
4.005AspVal: 4.005 ± 0.108
0.587AspTrp: 0.587 ± 0.038
2.371AspTyr: 2.371 ± 0.074
0.0AspXaa: 0.0 ± 0.0
Glu
3.965GluAla: 3.965 ± 0.122
0.384GluCys: 0.384 ± 0.029
3.483GluAsp: 3.483 ± 0.101
6.181GluGlu: 6.181 ± 0.142
2.599GluPhe: 2.599 ± 0.078
4.292GluGly: 4.292 ± 0.106
0.713GluHis: 0.713 ± 0.043
7.287GluIle: 7.287 ± 0.141
6.121GluLys: 6.121 ± 0.151
6.725GluLeu: 6.725 ± 0.14
1.614GluMet: 1.614 ± 0.058
3.166GluAsn: 3.166 ± 0.102
1.797GluPro: 1.797 ± 0.063
1.158GluGln: 1.158 ± 0.055
2.921GluArg: 2.921 ± 0.085
3.176GluSer: 3.176 ± 0.082
2.347GluThr: 2.347 ± 0.08
5.79GluVal: 5.79 ± 0.13
0.728GluTrp: 0.728 ± 0.043
3.178GluTyr: 3.178 ± 0.089
0.0GluXaa: 0.0 ± 0.0
Phe
2.369PheAla: 2.369 ± 0.096
0.233PheCys: 0.233 ± 0.025
2.047PheAsp: 2.047 ± 0.076
2.29PheGlu: 2.29 ± 0.075
1.975PhePhe: 1.975 ± 0.091
2.782PheGly: 2.782 ± 0.085
0.757PheHis: 0.757 ± 0.044
4.116PheIle: 4.116 ± 0.116
2.963PheLys: 2.963 ± 0.083
4.874PheLeu: 4.874 ± 0.123
1.092PheMet: 1.092 ± 0.052
2.567PheAsn: 2.567 ± 0.079
2.003PhePro: 2.003 ± 0.076
0.948PheGln: 0.948 ± 0.053
1.735PheArg: 1.735 ± 0.061
3.663PheSer: 3.663 ± 0.11
2.262PheThr: 2.262 ± 0.073
2.785PheVal: 2.785 ± 0.085
0.446PheTrp: 0.446 ± 0.037
2.191PheTyr: 2.191 ± 0.076
0.0PheXaa: 0.0 ± 0.0
Gly
3.408GlyAla: 3.408 ± 0.12
0.46GlyCys: 0.46 ± 0.041
3.072GlyAsp: 3.072 ± 0.1
4.371GlyGlu: 4.371 ± 0.108
3.334GlyPhe: 3.334 ± 0.089
4.567GlyGly: 4.567 ± 0.132
0.98GlyHis: 0.98 ± 0.045
7.431GlyIle: 7.431 ± 0.163
5.644GlyLys: 5.644 ± 0.126
6.344GlyLeu: 6.344 ± 0.133
1.634GlyMet: 1.634 ± 0.076
3.042GlyAsn: 3.042 ± 0.09
1.757GlyPro: 1.757 ± 0.064
1.111GlyGln: 1.111 ± 0.048
2.785GlyArg: 2.785 ± 0.093
4.025GlySer: 4.025 ± 0.1
2.901GlyThr: 2.901 ± 0.085
5.094GlyVal: 5.094 ± 0.125
0.849GlyTrp: 0.849 ± 0.05
3.579GlyTyr: 3.579 ± 0.092
0.0GlyXaa: 0.0 ± 0.0
His
0.75HisAla: 0.75 ± 0.043
0.114HisCys: 0.114 ± 0.019
0.639HisAsp: 0.639 ± 0.037
0.847HisGlu: 0.847 ± 0.043
0.584HisPhe: 0.584 ± 0.034
1.101HisGly: 1.101 ± 0.062
0.282HisHis: 0.282 ± 0.028
1.047HisIle: 1.047 ± 0.048
0.824HisLys: 0.824 ± 0.045
1.312HisLeu: 1.312 ± 0.059
0.28HisMet: 0.28 ± 0.026
0.517HisAsn: 0.517 ± 0.035
0.822HisPro: 0.822 ± 0.041
0.25HisGln: 0.25 ± 0.024
0.525HisArg: 0.525 ± 0.036
0.819HisSer: 0.819 ± 0.046
0.589HisThr: 0.589 ± 0.037
0.988HisVal: 0.988 ± 0.051
0.146HisTrp: 0.146 ± 0.018
0.636HisTyr: 0.636 ± 0.043
0.0HisXaa: 0.0 ± 0.0
Ile
6.312IleAla: 6.312 ± 0.128
0.54IleCys: 0.54 ± 0.033
5.032IleAsp: 5.032 ± 0.122
5.956IleGlu: 5.956 ± 0.133
3.993IlePhe: 3.993 ± 0.116
6.478IleGly: 6.478 ± 0.161
1.106IleHis: 1.106 ± 0.048
9.646IleIle: 9.646 ± 0.198
7.119IleLys: 7.119 ± 0.16
9.683IleLeu: 9.683 ± 0.194
1.988IleMet: 1.988 ± 0.076
5.094IleAsn: 5.094 ± 0.134
4.181IlePro: 4.181 ± 0.109
1.871IleGln: 1.871 ± 0.06
4.418IleArg: 4.418 ± 0.108
7.772IleSer: 7.772 ± 0.166
5.01IleThr: 5.01 ± 0.099
6.911IleVal: 6.911 ± 0.136
0.688IleTrp: 0.688 ± 0.043
4.203IleTyr: 4.203 ± 0.112
0.0IleXaa: 0.0 ± 0.0
Lys
3.955LysAla: 3.955 ± 0.106
0.537LysCys: 0.537 ± 0.044
4.282LysAsp: 4.282 ± 0.111
6.426LysGlu: 6.426 ± 0.124
2.787LysPhe: 2.787 ± 0.096
5.146LysGly: 5.146 ± 0.111
0.703LysHis: 0.703 ± 0.045
7.701LysIle: 7.701 ± 0.16
5.411LysLys: 5.411 ± 0.15
7.22LysLeu: 7.22 ± 0.141
1.683LysMet: 1.683 ± 0.062
3.418LysAsn: 3.418 ± 0.086
2.708LysPro: 2.708 ± 0.081
1.465LysGln: 1.465 ± 0.064
3.634LysArg: 3.634 ± 0.09
4.205LysSer: 4.205 ± 0.11
2.748LysThr: 2.748 ± 0.081
6.807LysVal: 6.807 ± 0.143
0.797LysTrp: 0.797 ± 0.042
3.809LysTyr: 3.809 ± 0.105
0.0LysXaa: 0.0 ± 0.0
Leu
6.594LeuAla: 6.594 ± 0.151
0.512LeuCys: 0.512 ± 0.038
5.072LeuAsp: 5.072 ± 0.116
6.413LeuGlu: 6.413 ± 0.149
4.201LeuPhe: 4.201 ± 0.124
7.693LeuGly: 7.693 ± 0.15
1.356LeuHis: 1.356 ± 0.062
9.119LeuIle: 9.119 ± 0.194
7.27LeuLys: 7.27 ± 0.142
9.871LeuLeu: 9.871 ± 0.209
2.151LeuMet: 2.151 ± 0.069
5.302LeuAsn: 5.302 ± 0.121
4.354LeuPro: 4.354 ± 0.105
1.978LeuGln: 1.978 ± 0.067
4.876LeuArg: 4.876 ± 0.115
7.97LeuSer: 7.97 ± 0.148
5.022LeuThr: 5.022 ± 0.103
7.156LeuVal: 7.156 ± 0.134
0.879LeuTrp: 0.879 ± 0.045
4.292LeuTyr: 4.292 ± 0.101
0.0LeuXaa: 0.0 ± 0.0
Met
1.384MetAla: 1.384 ± 0.065
0.121MetCys: 0.121 ± 0.018
1.005MetAsp: 1.005 ± 0.048
1.421MetGlu: 1.421 ± 0.059
0.839MetPhe: 0.839 ± 0.044
1.421MetGly: 1.421 ± 0.062
0.275MetHis: 0.275 ± 0.026
2.116MetIle: 2.116 ± 0.072
2.074MetLys: 2.074 ± 0.073
2.181MetLeu: 2.181 ± 0.07
0.589MetMet: 0.589 ± 0.046
1.074MetAsn: 1.074 ± 0.046
0.931MetPro: 0.931 ± 0.046
0.451MetGln: 0.451 ± 0.036
1.203MetArg: 1.203 ± 0.052
1.715MetSer: 1.715 ± 0.071
0.995MetThr: 0.995 ± 0.047
1.314MetVal: 1.314 ± 0.049
0.29MetTrp: 0.29 ± 0.028
0.728MetTyr: 0.728 ± 0.043
0.0MetXaa: 0.0 ± 0.0
Asn
2.886AsnAla: 2.886 ± 0.085
0.349AsnCys: 0.349 ± 0.031
2.408AsnAsp: 2.408 ± 0.091
3.451AsnGlu: 3.451 ± 0.095
2.139AsnPhe: 2.139 ± 0.074
3.205AsnGly: 3.205 ± 0.093
0.512AsnHis: 0.512 ± 0.036
4.465AsnIle: 4.465 ± 0.121
3.671AsnLys: 3.671 ± 0.098
5.089AsnLeu: 5.089 ± 0.129
0.921AsnMet: 0.921 ± 0.045
2.653AsnAsn: 2.653 ± 0.094
2.488AsnPro: 2.488 ± 0.071
1.106AsnGln: 1.106 ± 0.054
1.797AsnArg: 1.797 ± 0.063
3.505AsnSer: 3.505 ± 0.098
2.156AsnThr: 2.156 ± 0.084
4.342AsnVal: 4.342 ± 0.114
0.554AsnTrp: 0.554 ± 0.043
2.582AsnTyr: 2.582 ± 0.085
0.0AsnXaa: 0.0 ± 0.0
Pro
1.951ProAla: 1.951 ± 0.071
0.23ProCys: 0.23 ± 0.025
1.705ProAsp: 1.705 ± 0.07
2.426ProGlu: 2.426 ± 0.089
2.149ProPhe: 2.149 ± 0.076
2.106ProGly: 2.106 ± 0.077
0.619ProHis: 0.619 ± 0.038
3.822ProIle: 3.822 ± 0.084
2.765ProLys: 2.765 ± 0.083
4.106ProLeu: 4.106 ± 0.122
0.792ProMet: 0.792 ± 0.045
2.01ProAsn: 2.01 ± 0.072
1.871ProPro: 1.871 ± 0.078
1.082ProGln: 1.082 ± 0.057
1.502ProArg: 1.502 ± 0.059
3.0ProSer: 3.0 ± 0.095
2.027ProThr: 2.027 ± 0.072
2.512ProVal: 2.512 ± 0.083
0.493ProTrp: 0.493 ± 0.035
2.24ProTyr: 2.24 ± 0.073
0.0ProXaa: 0.0 ± 0.0
Gln
1.235GlnAla: 1.235 ± 0.061
0.089GlnCys: 0.089 ± 0.016
0.901GlnAsp: 0.901 ± 0.045
1.22GlnGlu: 1.22 ± 0.053
0.886GlnPhe: 0.886 ± 0.047
1.488GlnGly: 1.488 ± 0.061
0.255GlnHis: 0.255 ± 0.027
1.988GlnIle: 1.988 ± 0.068
1.347GlnLys: 1.347 ± 0.062
2.158GlnLeu: 2.158 ± 0.086
0.525GlnMet: 0.525 ± 0.035
1.059GlnAsn: 1.059 ± 0.052
0.76GlnPro: 0.76 ± 0.044
0.666GlnGln: 0.666 ± 0.049
1.017GlnArg: 1.017 ± 0.055
1.022GlnSer: 1.022 ± 0.055
0.901GlnThr: 0.901 ± 0.053
1.639GlnVal: 1.639 ± 0.071
0.205GlnTrp: 0.205 ± 0.02
0.884GlnTyr: 0.884 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.374ArgAla: 2.374 ± 0.077
0.329ArgCys: 0.329 ± 0.032
2.416ArgAsp: 2.416 ± 0.091
3.713ArgGlu: 3.713 ± 0.102
1.743ArgPhe: 1.743 ± 0.066
3.208ArgGly: 3.208 ± 0.108
0.502ArgHis: 0.502 ± 0.032
4.007ArgIle: 4.007 ± 0.107
3.517ArgLys: 3.517 ± 0.097
4.188ArgLeu: 4.188 ± 0.092
0.874ArgMet: 0.874 ± 0.049
2.079ArgAsn: 2.079 ± 0.065
1.354ArgPro: 1.354 ± 0.061
0.973ArgGln: 0.973 ± 0.058
2.701ArgArg: 2.701 ± 0.086
2.186ArgSer: 2.186 ± 0.067
1.559ArgThr: 1.559 ± 0.059
3.448ArgVal: 3.448 ± 0.101
0.522ArgTrp: 0.522 ± 0.04
1.955ArgTyr: 1.955 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
3.5SerAla: 3.5 ± 0.098
0.374SerCys: 0.374 ± 0.031
2.797SerAsp: 2.797 ± 0.093
4.064SerGlu: 4.064 ± 0.097
3.29SerPhe: 3.29 ± 0.094
4.151SerGly: 4.151 ± 0.108
1.054SerHis: 1.054 ± 0.056
6.607SerIle: 6.607 ± 0.132
5.035SerLys: 5.035 ± 0.103
8.003SerLeu: 8.003 ± 0.186
1.559SerMet: 1.559 ± 0.067
3.399SerAsn: 3.399 ± 0.098
2.921SerPro: 2.921 ± 0.078
1.757SerGln: 1.757 ± 0.069
2.629SerArg: 2.629 ± 0.085
5.359SerSer: 5.359 ± 0.135
3.483SerThr: 3.483 ± 0.114
4.507SerVal: 4.507 ± 0.094
0.723SerTrp: 0.723 ± 0.041
3.136SerTyr: 3.136 ± 0.111
0.0SerXaa: 0.0 ± 0.0
Thr
2.713ThrAla: 2.713 ± 0.081
0.304ThrCys: 0.304 ± 0.028
1.819ThrAsp: 1.819 ± 0.058
2.196ThrGlu: 2.196 ± 0.077
2.3ThrPhe: 2.3 ± 0.074
3.121ThrGly: 3.121 ± 0.101
0.725ThrHis: 0.725 ± 0.043
4.555ThrIle: 4.555 ± 0.129
2.547ThrLys: 2.547 ± 0.078
5.334ThrLeu: 5.334 ± 0.112
0.941ThrMet: 0.941 ± 0.053
2.082ThrAsn: 2.082 ± 0.074
2.225ThrPro: 2.225 ± 0.083
0.955ThrGln: 0.955 ± 0.052
1.686ThrArg: 1.686 ± 0.054
3.468ThrSer: 3.468 ± 0.105
2.688ThrThr: 2.688 ± 0.124
3.193ThrVal: 3.193 ± 0.093
0.512ThrTrp: 0.512 ± 0.036
2.126ThrTyr: 2.126 ± 0.077
0.0ThrXaa: 0.0 ± 0.0
Val
4.064ValAla: 4.064 ± 0.111
0.483ValCys: 0.483 ± 0.041
3.733ValAsp: 3.733 ± 0.098
4.911ValGlu: 4.911 ± 0.11
3.114ValPhe: 3.114 ± 0.095
4.651ValGly: 4.651 ± 0.123
0.97ValHis: 0.97 ± 0.05
7.866ValIle: 7.866 ± 0.156
6.545ValLys: 6.545 ± 0.134
6.958ValLeu: 6.958 ± 0.148
1.678ValMet: 1.678 ± 0.066
4.406ValAsn: 4.406 ± 0.117
2.671ValPro: 2.671 ± 0.078
1.257ValGln: 1.257 ± 0.062
3.285ValArg: 3.285 ± 0.095
5.24ValSer: 5.24 ± 0.122
3.483ValThr: 3.483 ± 0.103
5.562ValVal: 5.562 ± 0.155
0.644ValTrp: 0.644 ± 0.042
3.322ValTyr: 3.322 ± 0.097
0.0ValXaa: 0.0 ± 0.0
Trp
0.599TrpAla: 0.599 ± 0.038
0.052TrpCys: 0.052 ± 0.013
0.562TrpAsp: 0.562 ± 0.042
0.599TrpGlu: 0.599 ± 0.041
0.47TrpPhe: 0.47 ± 0.041
0.74TrpGly: 0.74 ± 0.045
0.171TrpHis: 0.171 ± 0.022
0.96TrpIle: 0.96 ± 0.053
0.814TrpLys: 0.814 ± 0.046
1.097TrpLeu: 1.097 ± 0.053
0.223TrpMet: 0.223 ± 0.023
0.572TrpAsn: 0.572 ± 0.041
0.28TrpPro: 0.28 ± 0.03
0.191TrpGln: 0.191 ± 0.025
0.564TrpArg: 0.564 ± 0.035
0.698TrpSer: 0.698 ± 0.043
0.505TrpThr: 0.505 ± 0.036
0.646TrpVal: 0.646 ± 0.043
0.173TrpTrp: 0.173 ± 0.026
0.54TrpTyr: 0.54 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.686TyrAla: 2.686 ± 0.085
0.396TyrCys: 0.396 ± 0.038
2.602TyrAsp: 2.602 ± 0.089
2.965TyrGlu: 2.965 ± 0.101
2.29TyrPhe: 2.29 ± 0.074
3.01TyrGly: 3.01 ± 0.085
0.636TyrHis: 0.636 ± 0.041
3.889TyrIle: 3.889 ± 0.087
3.012TyrLys: 3.012 ± 0.092
4.98TyrLeu: 4.98 ± 0.128
1.045TyrMet: 1.045 ± 0.046
2.767TyrAsn: 2.767 ± 0.082
1.918TyrPro: 1.918 ± 0.058
0.978TyrGln: 0.978 ± 0.054
1.775TyrArg: 1.775 ± 0.069
3.295TyrSer: 3.295 ± 0.105
2.376TyrThr: 2.376 ± 0.088
3.76TyrVal: 3.76 ± 0.09
0.601TyrTrp: 0.601 ± 0.042
2.705TyrTyr: 2.705 ± 0.095
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1702 proteins (403996 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski