Liberibacter phage SC1
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7DUI0|E7DUI0_9CAUD Uncharacterized protein OS=Liberibacter phage SC1 OX=941969 GN=SC1_gp190 PE=4 SV=1
MM1 pKa = 7.48 GALKK5 pKa = 10.6 NHH7 pKa = 6.01 FHH9 pKa = 7.93 DD10 pKa = 5.19 EE11 pKa = 4.16 INEE14 pKa = 3.7 NFYY17 pKa = 11.02 FHH19 pKa = 6.71 SHH21 pKa = 6.94 PNADD25 pKa = 3.54 PDD27 pKa = 3.9 ISIEE31 pKa = 4.02 MQISEE36 pKa = 4.21 NQRR39 pKa = 11.84 YY40 pKa = 9.2 LDD42 pKa = 4.37 EE43 pKa = 5.99 EE44 pKa = 4.27 ISQCNAVVDD53 pKa = 3.79 VFKK56 pKa = 11.01 RR57 pKa = 11.84 SDD59 pKa = 3.32 STILDD64 pKa = 3.6 KK65 pKa = 11.4 LDD67 pKa = 4.08 AVDD70 pKa = 5.19 DD71 pKa = 4.32 LKK73 pKa = 10.54 TYY75 pKa = 10.69 ISLLQATAKK84 pKa = 10.16 NLKK87 pKa = 10.12 SLLKK91 pKa = 10.06 EE92 pKa = 3.88 YY93 pKa = 10.14 WEE95 pKa = 4.41 EE96 pKa = 4.11 SLDD99 pKa = 4.21 GEE101 pKa = 4.55 DD102 pKa = 4.66 DD103 pKa = 3.84 EE104 pKa = 6.1 EE105 pKa = 5.64 IYY107 pKa = 10.58 EE108 pKa = 5.08 HH109 pKa = 7.59 PDD111 pKa = 2.95 QEE113 pKa = 4.6 HH114 pKa = 6.7 RR115 pKa = 11.84 EE116 pKa = 4.1 DD117 pKa = 4.28 YY118 pKa = 10.37 YY119 pKa = 11.83 ANQII123 pKa = 3.68
Molecular weight: 14.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.202
IPC2_protein 4.24
IPC_protein 4.19
Toseland 4.024
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.062
Rodwell 4.037
Grimsley 3.935
Solomon 4.151
Lehninger 4.113
Nozaki 4.279
DTASelect 4.457
Thurlkill 4.037
EMBOSS 4.075
Sillero 4.317
Patrickios 3.236
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.219
Protein with the highest isoelectric point:
>tr|E7DUH5|E7DUH5_9CAUD Uncharacterized protein OS=Liberibacter phage SC1 OX=941969 GN=SC1_gp160 PE=4 SV=1
MM1 pKa = 7.36 FCYY4 pKa = 9.87 PLKK7 pKa = 10.39 TNAHH11 pKa = 6.36 CKK13 pKa = 9.81 GALKK17 pKa = 10.54 RR18 pKa = 11.84 GTLKK22 pKa = 10.21 TEE24 pKa = 3.88 GGIQKK29 pKa = 9.11 VRR31 pKa = 11.84 SKK33 pKa = 11.16 GVAKK37 pKa = 10.14 HH38 pKa = 6.27 HH39 pKa = 5.92 PLKK42 pKa = 10.42 TEE44 pKa = 3.8 GGIQKK49 pKa = 9.06 VRR51 pKa = 11.84 SKK53 pKa = 11.11 GAVKK57 pKa = 10.16 HH58 pKa = 5.85 RR59 pKa = 11.84 PPP61 pKa = 4.79
Molecular weight: 6.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.237
IPC2_protein 9.853
IPC_protein 10.014
Toseland 11.023
ProMoST 10.496
Dawson 11.067
Bjellqvist 10.672
Wikipedia 11.184
Rodwell 11.681
Grimsley 11.082
Solomon 11.14
Lehninger 11.125
Nozaki 10.994
DTASelect 10.657
Thurlkill 10.979
EMBOSS 11.389
Sillero 10.994
Patrickios 11.418
IPC_peptide 11.155
IPC2_peptide 9.414
IPC2.peptide.svr19 8.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
40
0
40
12124
61
1340
303.1
34.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.739 ± 0.363
0.949 ± 0.17
6.285 ± 0.275
7.638 ± 0.427
3.769 ± 0.281
5.757 ± 0.254
2.276 ± 0.195
5.922 ± 0.327
8.157 ± 0.402
9.378 ± 0.4
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.186 ± 0.164
4.124 ± 0.24
3.712 ± 0.265
4.124 ± 0.279
5.708 ± 0.248
7.357 ± 0.29
5.394 ± 0.204
5.988 ± 0.241
1.336 ± 0.139
3.2 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here