Hyphopichia burtonii NRRL Y-1933

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Hyphopichia; Hyphopichia burtonii

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5994 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E4RJN4|A0A1E4RJN4_9ASCO Uncharacterized protein OS=Hyphopichia burtonii NRRL Y-1933 OX=984485 GN=HYPBUDRAFT_147823 PE=4 SV=1
MM1 pKa = 7.5ILSILTSLSVLMTVTAFISNEE22 pKa = 3.76QLLPTLISNYY32 pKa = 9.43RR33 pKa = 11.84DD34 pKa = 3.03SGLNQDD40 pKa = 3.16QLTYY44 pKa = 10.7IFLQNQVLDD53 pKa = 4.09NEE55 pKa = 4.71SDD57 pKa = 3.65DD58 pKa = 4.17TTSLLLDD65 pKa = 3.91NGVVLPLTQPANDD78 pKa = 3.4SHH80 pKa = 6.75IASISNWFNDD90 pKa = 3.0HH91 pKa = 6.61SAVFDD96 pKa = 4.28SNLPYY101 pKa = 10.92YY102 pKa = 10.27NSTVAYY108 pKa = 10.5DD109 pKa = 3.4NGEE112 pKa = 4.11EE113 pKa = 4.1VNPEE117 pKa = 3.9VISRR121 pKa = 11.84LRR123 pKa = 11.84EE124 pKa = 3.62NLGFGTSSLLLTPTATNLILFWNADD149 pKa = 3.18KK150 pKa = 11.3YY151 pKa = 11.45

Molecular weight: 16.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E4RFE4|A0A1E4RFE4_9ASCO GPI ethanolamine phosphate transferase 1 OS=Hyphopichia burtonii NRRL Y-1933 OX=984485 GN=HYPBUDRAFT_142010 PE=3 SV=1
SS1 pKa = 6.45QKK3 pKa = 9.29TFKK6 pKa = 10.81VKK8 pKa = 10.47QKK10 pKa = 10.02LAKK13 pKa = 9.66AQKK16 pKa = 9.21QNRR19 pKa = 11.84PLPQWIRR26 pKa = 11.84LRR28 pKa = 11.84SGNTIRR34 pKa = 11.84YY35 pKa = 5.78NAKK38 pKa = 8.47RR39 pKa = 11.84RR40 pKa = 11.84HH41 pKa = 4.11WRR43 pKa = 11.84RR44 pKa = 11.84TKK46 pKa = 11.12LNII49 pKa = 3.83

Molecular weight: 6.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5993

1

5994

2810767

49

4956

468.9

53.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.125 ± 0.031

1.053 ± 0.011

6.121 ± 0.023

6.453 ± 0.035

4.6 ± 0.022

5.047 ± 0.032

2.136 ± 0.013

7.107 ± 0.026

7.502 ± 0.031

9.85 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.725 ± 0.011

6.739 ± 0.035

4.506 ± 0.025

4.054 ± 0.023

3.842 ± 0.021

9.047 ± 0.041

5.279 ± 0.018

5.257 ± 0.024

1.002 ± 0.009

3.554 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski