Donghicola eburneus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Donghicola

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4MXU6|A0A1M4MXU6_9RHOB Putative MobA protein OS=Donghicola eburneus OX=393278 GN=mobA1 PE=4 SV=1
MM1 pKa = 7.12STTFQVMYY9 pKa = 10.18LGQLALIDD17 pKa = 4.05SVQGDD22 pKa = 3.89EE23 pKa = 4.13TAEE26 pKa = 3.87NAGAILGSYY35 pKa = 9.75YY36 pKa = 10.9NSTDD40 pKa = 3.08PAYY43 pKa = 10.84DD44 pKa = 3.06HH45 pKa = 7.25TYY47 pKa = 10.96QLTADD52 pKa = 4.57RR53 pKa = 11.84LSEE56 pKa = 4.53DD57 pKa = 4.08ADD59 pKa = 3.56DD60 pKa = 5.34TYY62 pKa = 11.91DD63 pKa = 3.28VDD65 pKa = 4.33NGGGHH70 pKa = 7.32DD71 pKa = 3.46RR72 pKa = 11.84FFVNGTGPYY81 pKa = 10.58DD82 pKa = 3.93FDD84 pKa = 5.07AVAEE88 pKa = 4.06YY89 pKa = 10.57QITLTYY95 pKa = 10.24IDD97 pKa = 4.22GTTANITAYY106 pKa = 10.31VFQDD110 pKa = 3.53TTGHH114 pKa = 5.41TFIAPEE120 pKa = 3.89TTYY123 pKa = 11.35NADD126 pKa = 3.1QAALTDD132 pKa = 4.1KK133 pKa = 10.59PIQSVSLNSVTSYY146 pKa = 10.6TGDD149 pKa = 3.18KK150 pKa = 10.39DD151 pKa = 3.62GDD153 pKa = 4.11MIASRR158 pKa = 11.84VNEE161 pKa = 4.4PYY163 pKa = 10.62AGPVDD168 pKa = 3.87GTNGNDD174 pKa = 3.37SMGVGYY180 pKa = 9.35TDD182 pKa = 3.62AQGHH186 pKa = 7.32AITEE190 pKa = 4.34DD191 pKa = 3.38ADD193 pKa = 3.76VIYY196 pKa = 9.92GYY198 pKa = 10.86DD199 pKa = 3.54GADD202 pKa = 3.58TIDD205 pKa = 3.79GGGGSDD211 pKa = 5.1LIEE214 pKa = 5.04SGAGSDD220 pKa = 3.65VVYY223 pKa = 10.77GGAGNDD229 pKa = 3.54TIYY232 pKa = 11.09YY233 pKa = 7.61GTGNDD238 pKa = 3.87TIYY241 pKa = 11.2GGDD244 pKa = 3.91GNDD247 pKa = 5.62LIDD250 pKa = 5.33DD251 pKa = 4.2EE252 pKa = 6.39DD253 pKa = 4.01GVRR256 pKa = 11.84YY257 pKa = 8.12TGSNEE262 pKa = 3.46IHH264 pKa = 6.41GGEE267 pKa = 4.47GNDD270 pKa = 3.45TVYY273 pKa = 11.01SGDD276 pKa = 3.52GSEE279 pKa = 4.58TIYY282 pKa = 11.37GDD284 pKa = 4.03GGDD287 pKa = 4.43DD288 pKa = 3.52NLFGEE293 pKa = 5.17GGNDD297 pKa = 3.29TLFGGTGNDD306 pKa = 3.74VLWGGSGDD314 pKa = 4.35DD315 pKa = 3.87YY316 pKa = 11.74LAGEE320 pKa = 5.38DD321 pKa = 4.02GNDD324 pKa = 3.38WLVGEE329 pKa = 5.21AGNDD333 pKa = 3.7TLIGGAGNDD342 pKa = 3.5SLYY345 pKa = 11.18GGTGNDD351 pKa = 3.41SMLGGDD357 pKa = 4.34GDD359 pKa = 3.82DD360 pKa = 3.27TFYY363 pKa = 11.58YY364 pKa = 10.42FAGDD368 pKa = 3.74GLDD371 pKa = 3.86TIADD375 pKa = 3.83FGFGNTGSTTDD386 pKa = 4.87GISTNNDD393 pKa = 3.53FIDD396 pKa = 3.88LSSYY400 pKa = 9.61YY401 pKa = 10.78DD402 pKa = 3.28AMNEE406 pKa = 3.68LRR408 pKa = 11.84ADD410 pKa = 3.82FLDD413 pKa = 5.51DD414 pKa = 5.58GILNQSNAADD424 pKa = 3.8YY425 pKa = 11.63SNNTQFQPGDD435 pKa = 3.63GIVFQGATTSDD446 pKa = 3.57FTTDD450 pKa = 2.95STGVVCFTTGTQIRR464 pKa = 11.84TPDD467 pKa = 3.34GDD469 pKa = 3.77RR470 pKa = 11.84PIEE473 pKa = 3.93EE474 pKa = 4.87LKK476 pKa = 11.27VGDD479 pKa = 4.69LVEE482 pKa = 4.44TLDD485 pKa = 5.55GGPQPIKK492 pKa = 10.32WIGTSHH498 pKa = 6.7YY499 pKa = 10.93DD500 pKa = 3.3EE501 pKa = 5.27AALIEE506 pKa = 4.21NPKK509 pKa = 9.99LRR511 pKa = 11.84PIVVSARR518 pKa = 11.84VFGAEE523 pKa = 3.54RR524 pKa = 11.84DD525 pKa = 3.96LVVSRR530 pKa = 11.84QHH532 pKa = 6.91AFLLPKK538 pKa = 10.21EE539 pKa = 4.49DD540 pKa = 3.75LLARR544 pKa = 11.84AIQLVKK550 pKa = 10.44MNVAGVRR557 pKa = 11.84IAHH560 pKa = 5.28GRR562 pKa = 11.84KK563 pKa = 8.72RR564 pKa = 11.84VTYY567 pKa = 8.41HH568 pKa = 6.71HH569 pKa = 6.94IMFEE573 pKa = 3.95KK574 pKa = 10.75HH575 pKa = 4.67EE576 pKa = 4.79LIYY579 pKa = 10.92SEE581 pKa = 5.61GIATEE586 pKa = 4.04SMYY589 pKa = 10.52PGPQALRR596 pKa = 11.84ALCPEE601 pKa = 3.94AMEE604 pKa = 4.28SLLRR608 pKa = 11.84VFPEE612 pKa = 4.18FKK614 pKa = 10.58GIEE617 pKa = 4.07TRR619 pKa = 11.84EE620 pKa = 4.15GAEE623 pKa = 3.65AAYY626 pKa = 10.17GPTARR631 pKa = 11.84EE632 pKa = 4.26MIDD635 pKa = 3.15MSNWRR640 pKa = 11.84AEE642 pKa = 3.89AAAA645 pKa = 4.4

Molecular weight: 68.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4N4T5|A0A1M4N4T5_9RHOB Uncharacterized protein OS=Donghicola eburneus OX=393278 GN=KARMA_3320 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4047

0

4047

1262338

30

1800

311.9

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.628 ± 0.052

0.914 ± 0.013

6.166 ± 0.04

6.199 ± 0.036

3.707 ± 0.024

8.455 ± 0.039

2.027 ± 0.02

5.392 ± 0.025

3.521 ± 0.033

9.815 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.944 ± 0.015

2.843 ± 0.022

4.808 ± 0.027

3.376 ± 0.023

6.211 ± 0.039

5.42 ± 0.026

5.659 ± 0.028

7.193 ± 0.032

1.348 ± 0.016

2.372 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski