Microvirga aerophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7161 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A512C525|A0A512C525_9RHIZ IS110 family transposase OS=Microvirga aerophila OX=670291 GN=MAE02_70050 PE=4 SV=1
MM1 pKa = 7.95AILLGNDD8 pKa = 3.21WPNLIRR14 pKa = 11.84GTNASDD20 pKa = 4.12SIWGYY25 pKa = 11.63GDD27 pKa = 5.87DD28 pKa = 4.85DD29 pKa = 4.99DD30 pKa = 6.81LYY32 pKa = 11.25GYY34 pKa = 10.89DD35 pKa = 4.8GDD37 pKa = 4.45DD38 pKa = 4.94DD39 pKa = 5.67IYY41 pKa = 11.38GGWGYY46 pKa = 11.3DD47 pKa = 3.3VLVGGNGDD55 pKa = 4.0DD56 pKa = 3.97YY57 pKa = 11.89LNGGDD62 pKa = 5.35GDD64 pKa = 4.47DD65 pKa = 4.53DD66 pKa = 5.55LYY68 pKa = 11.72GGAAQDD74 pKa = 3.9RR75 pKa = 11.84LIGGAGYY82 pKa = 10.7DD83 pKa = 3.76LLFGQSGNDD92 pKa = 3.51DD93 pKa = 4.53LYY95 pKa = 11.36GQSGYY100 pKa = 11.12DD101 pKa = 3.53DD102 pKa = 5.16LFGGSGLDD110 pKa = 3.59YY111 pKa = 11.31LNGGSSDD118 pKa = 3.66DD119 pKa = 3.77WLDD122 pKa = 3.73GGSGRR127 pKa = 11.84DD128 pKa = 3.31WLVGGTGVDD137 pKa = 2.29IFYY140 pKa = 10.36FKK142 pKa = 10.48RR143 pKa = 11.84GSSGITTASADD154 pKa = 4.06TIADD158 pKa = 3.26WRR160 pKa = 11.84PRR162 pKa = 11.84LDD164 pKa = 3.86WIDD167 pKa = 3.19MSIRR171 pKa = 11.84GTSGNHH177 pKa = 5.77RR178 pKa = 11.84EE179 pKa = 4.18ASTTATSIEE188 pKa = 3.95AAAAQAEE195 pKa = 4.55SRR197 pKa = 11.84FTSASIVHH205 pKa = 5.56VFLYY209 pKa = 10.49NAAADD214 pKa = 3.87RR215 pKa = 11.84GYY217 pKa = 11.39LLSDD221 pKa = 4.13LNNDD225 pKa = 2.91NRR227 pKa = 11.84YY228 pKa = 5.97EE229 pKa = 4.05TGVIILGAGRR239 pKa = 11.84AADD242 pKa = 3.58MDD244 pKa = 4.2YY245 pKa = 11.2RR246 pKa = 11.84WILL249 pKa = 3.35

Molecular weight: 26.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A512BW53|A0A512BW53_9RHIZ Uncharacterized protein OS=Microvirga aerophila OX=670291 GN=MAE02_38510 PE=4 SV=1
MM1 pKa = 7.13EE2 pKa = 4.1VSVRR6 pKa = 11.84EE7 pKa = 4.05VLSGITTMRR16 pKa = 11.84DD17 pKa = 2.96MVKK20 pKa = 10.52AFQNEE25 pKa = 3.96AHH27 pKa = 6.79CRR29 pKa = 11.84RR30 pKa = 11.84LVQAMVWPKK39 pKa = 10.88GRR41 pKa = 11.84ICPACGYY48 pKa = 9.46RR49 pKa = 11.84RR50 pKa = 11.84SIALTGRR57 pKa = 11.84EE58 pKa = 3.66NGKK61 pKa = 9.37RR62 pKa = 11.84ARR64 pKa = 11.84SGLYY68 pKa = 8.38QCSSGTCRR76 pKa = 11.84FQFTVTTRR84 pKa = 11.84TPLHH88 pKa = 5.73ATKK91 pKa = 10.73LPLRR95 pKa = 11.84TWLSGLWLMLQSDD108 pKa = 4.34KK109 pKa = 11.27GISSIRR115 pKa = 11.84LAEE118 pKa = 4.14ALGVSQPTAWRR129 pKa = 11.84MGHH132 pKa = 6.73ALRR135 pKa = 11.84LMVGRR140 pKa = 11.84EE141 pKa = 3.55RR142 pKa = 11.84ALDD145 pKa = 3.65GVVEE149 pKa = 4.01IDD151 pKa = 3.35GLYY154 pKa = 10.73LGGKK158 pKa = 7.69PRR160 pKa = 11.84RR161 pKa = 11.84EE162 pKa = 3.7VDD164 pKa = 3.22YY165 pKa = 10.97SPPGRR170 pKa = 11.84GRR172 pKa = 11.84KK173 pKa = 8.15GQPNDD178 pKa = 3.26LHH180 pKa = 6.71EE181 pKa = 5.36GGVTT185 pKa = 3.28

Molecular weight: 20.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7161

0

7161

1921182

39

3586

268.3

29.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.74 ± 0.035

0.815 ± 0.009

5.523 ± 0.028

5.864 ± 0.03

3.649 ± 0.02

8.397 ± 0.035

2.113 ± 0.015

5.173 ± 0.021

3.273 ± 0.027

10.193 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.014

2.616 ± 0.017

5.316 ± 0.02

3.376 ± 0.019

7.406 ± 0.037

5.648 ± 0.022

5.368 ± 0.025

7.528 ± 0.023

1.385 ± 0.012

2.251 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski