Saimiriine herpesvirus 2 (SaHV-2) (Herpesvirus saimiri)
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9Q1U7|Q9Q1U7_SHV2 ORF50b protein short form OS=Saimiriine herpesvirus 2 OX=10381 PE=4 SV=1
MM1 pKa = 7.29 NNEE4 pKa = 3.96 EE5 pKa = 4.14 EE6 pKa = 4.21 PVYY9 pKa = 11.0 EE10 pKa = 4.72 EE11 pKa = 3.82 IHH13 pKa = 6.32 PLRR16 pKa = 11.84 PQLVDD21 pKa = 3.62 TEE23 pKa = 4.5 NLDD26 pKa = 3.57 LDD28 pKa = 4.42 IYY30 pKa = 11.52 DD31 pKa = 4.39 DD32 pKa = 4.25 VCFLEE37 pKa = 5.02 DD38 pKa = 3.65 NSYY41 pKa = 11.93 DD42 pKa = 3.93 DD43 pKa = 4.3 ACCVYY48 pKa = 10.38 TPLKK52 pKa = 10.03 KK53 pKa = 10.67 SSVEE57 pKa = 3.77 YY58 pKa = 10.65 NQLKK62 pKa = 7.82 TQTEE66 pKa = 4.05 NLEE69 pKa = 3.74 VHH71 pKa = 6.55 IYY73 pKa = 10.49 EE74 pKa = 4.87 EE75 pKa = 4.53 IVV77 pKa = 2.99
Molecular weight: 9.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.86
IPC2_protein 3.884
IPC_protein 3.808
Toseland 3.63
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.643
Grimsley 3.541
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.037
Thurlkill 3.668
EMBOSS 3.694
Sillero 3.923
Patrickios 0.248
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|O40641|O40641_SHV2 Thymidine kinase OS=Saimiriine herpesvirus 2 OX=10381 GN=orf21 PE=3 SV=1
MM1 pKa = 7.48 LSVIKK6 pKa = 10.65 QRR8 pKa = 11.84 DD9 pKa = 3.82 KK10 pKa = 11.35 EE11 pKa = 4.44 VLAHH15 pKa = 6.66 LPNKK19 pKa = 10.23 RR20 pKa = 11.84 KK21 pKa = 9.73 IEE23 pKa = 4.03 GNKK26 pKa = 9.85 AHH28 pKa = 7.15 LEE30 pKa = 4.33 TYY32 pKa = 10.34 KK33 pKa = 10.94 KK34 pKa = 9.52 LAKK37 pKa = 8.8 YY38 pKa = 7.35 TVSASIFKK46 pKa = 10.11 FLSISHH52 pKa = 7.1 PCPLRR57 pKa = 11.84 AKK59 pKa = 8.15 TQLFFEE65 pKa = 4.61 VSLGNRR71 pKa = 11.84 IADD74 pKa = 3.5 CVMLTSCGEE83 pKa = 3.8 TRR85 pKa = 11.84 ICYY88 pKa = 9.91 VIEE91 pKa = 5.04 LKK93 pKa = 9.68 TCMTSNLDD101 pKa = 4.08 LISDD105 pKa = 3.47 IRR107 pKa = 11.84 KK108 pKa = 8.14 SQRR111 pKa = 11.84 SQGLCQLADD120 pKa = 3.54 TVNFIHH126 pKa = 6.91 NYY128 pKa = 9.98 APLGRR133 pKa = 11.84 QAWTVLPILIFKK145 pKa = 8.45 SQKK148 pKa = 7.13 TLKK151 pKa = 8.48 TLHH154 pKa = 6.58 IEE156 pKa = 4.28 TPKK159 pKa = 10.59 FPVNLTHH166 pKa = 6.84 TSEE169 pKa = 4.31 EE170 pKa = 4.15 KK171 pKa = 10.67 LSCFLWSRR179 pKa = 11.84 ADD181 pKa = 3.27 VEE183 pKa = 4.21 IRR185 pKa = 11.84 KK186 pKa = 9.68 KK187 pKa = 10.08 IHH189 pKa = 6.44 LAPKK193 pKa = 9.5 PKK195 pKa = 10.16 RR196 pKa = 11.84 ILKK199 pKa = 9.3 WDD201 pKa = 3.71 SLLDD205 pKa = 3.7 STSTEE210 pKa = 3.56 HH211 pKa = 6.05 SAYY214 pKa = 9.45 RR215 pKa = 11.84 QKK217 pKa = 11.03 LIEE220 pKa = 4.22 RR221 pKa = 11.84 NKK223 pKa = 10.25 KK224 pKa = 9.85 KK225 pKa = 10.71 CFTLQNQTSKK235 pKa = 10.7 FRR237 pKa = 11.84 DD238 pKa = 3.52 RR239 pKa = 11.84 PNKK242 pKa = 9.86 KK243 pKa = 10.71 GNDD246 pKa = 3.43 QLRR249 pKa = 11.84 ARR251 pKa = 11.84 QANARR256 pKa = 11.84 PCKK259 pKa = 10.09 KK260 pKa = 9.73 KK261 pKa = 9.4 QHH263 pKa = 5.06 NNKK266 pKa = 9.67 RR267 pKa = 11.84 LRR269 pKa = 11.84 NNRR272 pKa = 11.84 KK273 pKa = 9.16 HH274 pKa = 5.69 GGKK277 pKa = 9.77 VSRR280 pKa = 11.84 LATATSFFSEE290 pKa = 4.19 AAIPNYY296 pKa = 10.22 SVPTHH301 pKa = 6.0 KK302 pKa = 10.54 RR303 pKa = 3.06
Molecular weight: 34.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.202
IPC2_protein 9.472
IPC_protein 9.472
Toseland 10.54
ProMoST 10.058
Dawson 10.628
Bjellqvist 10.218
Wikipedia 10.73
Rodwell 11.228
Grimsley 10.643
Solomon 10.657
Lehninger 10.643
Nozaki 10.526
DTASelect 10.204
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.54
Patrickios 10.935
IPC_peptide 10.657
IPC2_peptide 8.931
IPC2.peptide.svr19 8.46
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
78
1
79
34276
66
2477
433.9
49.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.085 ± 0.201
2.728 ± 0.208
4.598 ± 0.215
5.89 ± 0.553
4.805 ± 0.177
3.901 ± 0.278
2.693 ± 0.096
7.206 ± 0.227
6.78 ± 0.274
10.331 ± 0.239
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.217 ± 0.104
5.549 ± 0.16
5.071 ± 0.231
3.898 ± 0.188
3.314 ± 0.183
8.423 ± 0.191
6.71 ± 0.241
5.972 ± 0.203
1.044 ± 0.064
3.784 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here