Hubei tombus-like virus 26

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG47|A0A1L3KG47_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 26 OX=1923273 PE=4 SV=1
MM1 pKa = 7.24SQDD4 pKa = 3.69EE5 pKa = 4.91YY6 pKa = 11.0IKK8 pKa = 10.11TMPPKK13 pKa = 9.43YY14 pKa = 10.17SRR16 pKa = 11.84FLGVPIYY23 pKa = 10.59NVNNNVKK30 pKa = 10.36CFIKK34 pKa = 10.43KK35 pKa = 9.97EE36 pKa = 4.2KK37 pKa = 10.25ISLTKK42 pKa = 8.99YY43 pKa = 6.14TAPRR47 pKa = 11.84LIQHH51 pKa = 5.71NTMAYY56 pKa = 8.24NIEE59 pKa = 3.72VGRR62 pKa = 11.84YY63 pKa = 8.46IKK65 pKa = 10.33QLEE68 pKa = 4.1HH69 pKa = 7.28KK70 pKa = 9.54IYY72 pKa = 10.8QKK74 pKa = 11.04DD75 pKa = 3.58SSSQKK80 pKa = 10.68YY81 pKa = 7.4EE82 pKa = 3.79FCKK85 pKa = 10.37TDD87 pKa = 3.6LPEE90 pKa = 3.59MARR93 pKa = 11.84RR94 pKa = 11.84IMYY97 pKa = 9.13KK98 pKa = 10.02ASKK101 pKa = 8.91FQNPKK106 pKa = 9.86FVCIDD111 pKa = 3.57HH112 pKa = 6.43KK113 pKa = 11.1QFEE116 pKa = 4.27AHH118 pKa = 7.01LNGQHH123 pKa = 6.59INLEE127 pKa = 3.9NAFINWHH134 pKa = 5.39YY135 pKa = 10.6RR136 pKa = 11.84SPYY139 pKa = 9.27LQRR142 pKa = 11.84LLDD145 pKa = 3.53ARR147 pKa = 11.84TIFGTTTSGIKK158 pKa = 10.21YY159 pKa = 6.15RR160 pKa = 11.84TSMTRR165 pKa = 11.84TSGHH169 pKa = 5.62VKK171 pKa = 9.67TSFGNSILNLAFIKK185 pKa = 10.56HH186 pKa = 5.29VLKK189 pKa = 10.46QLKK192 pKa = 8.78IRR194 pKa = 11.84KK195 pKa = 8.95FEE197 pKa = 4.09VMVNGDD203 pKa = 3.56DD204 pKa = 4.36SIIIIEE210 pKa = 4.3NRR212 pKa = 11.84CHH214 pKa = 7.32LNEE217 pKa = 4.25SQLKK221 pKa = 10.07RR222 pKa = 11.84EE223 pKa = 3.89FRR225 pKa = 11.84KK226 pKa = 10.66LNMEE230 pKa = 4.19TKK232 pKa = 9.79IDD234 pKa = 3.71QITNNIYY241 pKa = 10.46DD242 pKa = 3.61VEE244 pKa = 4.33FCRR247 pKa = 11.84MKK249 pKa = 10.47ICIDD253 pKa = 3.54NAGKK257 pKa = 9.85PMMYY261 pKa = 9.08LTPDD265 pKa = 4.08RR266 pKa = 11.84IKK268 pKa = 10.84KK269 pKa = 9.93IFAQTHH275 pKa = 4.91KK276 pKa = 10.56PMRR279 pKa = 11.84RR280 pKa = 11.84NDD282 pKa = 3.52YY283 pKa = 10.86VRR285 pKa = 11.84DD286 pKa = 3.31LAYY289 pKa = 10.56ANSCIYY295 pKa = 10.77KK296 pKa = 10.23NLEE299 pKa = 3.9NYY301 pKa = 10.2HH302 pKa = 6.61SVFTDD307 pKa = 3.46IYY309 pKa = 9.94NHH311 pKa = 5.99YY312 pKa = 10.27NSLATTQLNKK322 pKa = 10.34YY323 pKa = 9.74LKK325 pKa = 10.47LEE327 pKa = 3.95PLILSLYY334 pKa = 9.46KK335 pKa = 10.44AASPDD340 pKa = 3.57PRR342 pKa = 11.84VSDD345 pKa = 3.72PMPHH349 pKa = 5.09YY350 pKa = 7.08TQPKK354 pKa = 8.25LSPPRR359 pKa = 11.84SMPNFKK365 pKa = 10.49LAVPKK370 pKa = 10.64SKK372 pKa = 10.73LSAGSQRR379 pKa = 11.84FLSRR383 pKa = 11.84MVSSSRR389 pKa = 11.84KK390 pKa = 9.19FIFPEE395 pKa = 4.17VFQEE399 pKa = 3.85TDD401 pKa = 2.56ATNITRR407 pKa = 11.84HH408 pKa = 5.28VYY410 pKa = 10.01INHH413 pKa = 6.02HH414 pKa = 5.26TQTLTVTHH422 pKa = 7.04GPRR425 pKa = 11.84PPEE428 pKa = 3.97PTSFPNPNLPFPTPMM443 pKa = 4.86

Molecular weight: 51.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG47|A0A1L3KG47_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 26 OX=1923273 PE=4 SV=1
MM1 pKa = 7.79ALPKK5 pKa = 10.44ASEE8 pKa = 4.21TLSPQDD14 pKa = 3.75FPNPPKK20 pKa = 9.88TLAPIPSLPDD30 pKa = 3.48SLPKK34 pKa = 10.2SLKK37 pKa = 9.97HH38 pKa = 6.37SRR40 pKa = 11.84KK41 pKa = 8.27CTPGTFDD48 pKa = 3.31HH49 pKa = 6.49QRR51 pKa = 11.84LASEE55 pKa = 4.43LEE57 pKa = 4.23GVVTCNNTYY66 pKa = 8.1THH68 pKa = 6.74LHH70 pKa = 5.85DD71 pKa = 4.12TVNIHH76 pKa = 6.7TNLQDD81 pKa = 3.43EE82 pKa = 4.9LNAHH86 pKa = 6.88LEE88 pKa = 3.93LAMALKK94 pKa = 9.77PRR96 pKa = 11.84NVDD99 pKa = 4.23FYY101 pKa = 10.5RR102 pKa = 11.84QAIGEE107 pKa = 4.32SQNFLNRR114 pKa = 11.84YY115 pKa = 7.85NVNLPSARR123 pKa = 11.84LLLIRR128 pKa = 11.84NSLDD132 pKa = 3.26FLSSLTQTEE141 pKa = 4.0QLIHH145 pKa = 6.46SKK147 pKa = 9.71VEE149 pKa = 3.83KK150 pKa = 8.04TQKK153 pKa = 10.38FSGLKK158 pKa = 9.38YY159 pKa = 10.0IKK161 pKa = 10.04EE162 pKa = 4.07NSLSKK167 pKa = 10.85VNHH170 pKa = 6.35ILSKK174 pKa = 10.17IASRR178 pKa = 11.84PDD180 pKa = 3.23FRR182 pKa = 11.84LKK184 pKa = 10.56AHH186 pKa = 6.25VSRR189 pKa = 11.84SYY191 pKa = 11.56KK192 pKa = 10.56LYY194 pKa = 9.18TQLKK198 pKa = 8.3SHH200 pKa = 6.37IHH202 pKa = 4.6YY203 pKa = 10.45QYY205 pKa = 10.33TLTLIPITLIFSILTLPLTILRR227 pKa = 11.84ALQTRR232 pKa = 11.84WRR234 pKa = 11.84ASSTGTTTTVFLNPMFSYY252 pKa = 10.77VLPKK256 pKa = 10.36FSATSQNKK264 pKa = 8.35PLKK267 pKa = 10.42ASEE270 pKa = 3.67IYY272 pKa = 10.19LRR274 pKa = 11.84HH275 pKa = 4.97TAYY278 pKa = 10.56AEE280 pKa = 4.0TSAPCRR286 pKa = 11.84KK287 pKa = 9.14MNILKK292 pKa = 10.34LCLLSTLAFLVFLSIMLITMM312 pKa = 4.96

Molecular weight: 35.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

755

312

443

377.5

43.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.901 ± 0.717

1.457 ± 0.105

3.311 ± 0.451

3.974 ± 0.27

4.636 ± 0.283

2.119 ± 0.312

3.841 ± 0.003

6.887 ± 0.674

7.947 ± 0.733

10.861 ± 2.921

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.914 ± 0.597

6.49 ± 0.628

6.49 ± 0.241

3.974 ± 0.077

5.43 ± 0.376

8.212 ± 1.04

7.815 ± 0.893

3.841 ± 0.19

0.265 ± 0.034

4.636 ± 0.669
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski