Candidatus Phycosocius bacilliformis
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3121 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P2EDJ2|A0A2P2EDJ2_9PROT Uncharacterized protein OS=Candidatus Phycosocius bacilliformis OX=1445552 GN=PbB2_02819 PE=4 SV=1
MM1 pKa = 7.17 SQEE4 pKa = 4.09 QSNQAYY10 pKa = 9.49 YY11 pKa = 10.91 AGRR14 pKa = 11.84 TDD16 pKa = 3.19 AFNGVGQQTIADD28 pKa = 3.76 QSSNLQTDD36 pKa = 3.57 YY37 pKa = 11.58 DD38 pKa = 3.55 QGYY41 pKa = 9.78 RR42 pKa = 11.84 DD43 pKa = 4.1 ANSEE47 pKa = 3.95 IKK49 pKa = 10.47 LDD51 pKa = 5.24 QDD53 pKa = 4.31 TEE55 pKa = 3.84 WDD57 pKa = 3.32
Molecular weight: 6.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.681
IPC_protein 3.592
Toseland 3.389
ProMoST 3.706
Dawson 3.617
Bjellqvist 3.872
Wikipedia 3.617
Rodwell 3.439
Grimsley 3.312
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.999
Thurlkill 3.49
EMBOSS 3.617
Sillero 3.732
Patrickios 1.863
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A2P2EDU5|A0A2P2EDU5_9PROT NAD(P) transhydrogenase subunit alpha part 1 OS=Candidatus Phycosocius bacilliformis OX=1445552 GN=pntAA PE=3 SV=1
MM1 pKa = 7.5 GLACGCLTLSLALASPACAQSAKK24 pKa = 10.18 RR25 pKa = 11.84 GQEE28 pKa = 3.56 LTFNDD33 pKa = 3.76 MFEE36 pKa = 4.68 SPNAAAPQAVAFAAGPNNLRR56 pKa = 11.84 FVLDD60 pKa = 4.2 RR61 pKa = 11.84 SQPRR65 pKa = 11.84 VVLLQFDD72 pKa = 4.3 GDD74 pKa = 4.17 PEE76 pKa = 4.31 VWALTSSWGPRR87 pKa = 11.84 GDD89 pKa = 3.2 EE90 pKa = 4.45 FLRR93 pKa = 11.84 NDD95 pKa = 3.07 IGEE98 pKa = 4.33 VIVRR102 pKa = 11.84 ITTLGGVTVYY112 pKa = 11.1 GPLGTNGGLAASQGKK127 pKa = 9.65 AKK129 pKa = 10.41 SLPAPSRR136 pKa = 11.84 SEE138 pKa = 3.81 GTLQATLQKK147 pKa = 10.64 ALRR150 pKa = 11.84 WFDD153 pKa = 3.53 RR154 pKa = 11.84 FSSRR158 pKa = 11.84 SIRR161 pKa = 11.84 VEE163 pKa = 3.63 ASGGLPPALVHH174 pKa = 6.23 EE175 pKa = 4.62 ALEE178 pKa = 4.36 RR179 pKa = 11.84 AAQGMSRR186 pKa = 11.84 APRR189 pKa = 11.84 GYY191 pKa = 9.62 FGRR194 pKa = 11.84 PEE196 pKa = 4.07 RR197 pKa = 11.84 KK198 pKa = 8.74 VKK200 pKa = 10.68 LIRR203 pKa = 11.84 VQRR206 pKa = 11.84 SFDD209 pKa = 3.52 QPSVRR214 pKa = 11.84 WQSGVLTIGVVPGAGYY230 pKa = 10.7 AGRR233 pKa = 11.84 PSSAAVLLALTRR245 pKa = 11.84 SRR247 pKa = 4.21
Molecular weight: 26.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.458
IPC_protein 10.321
Toseland 10.584
ProMoST 10.57
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.76
Grimsley 10.73
Solomon 10.818
Lehninger 10.789
Nozaki 10.584
DTASelect 10.409
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.613
Patrickios 10.496
IPC_peptide 10.833
IPC2_peptide 9.589
IPC2.peptide.svr19 8.747
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3121
0
3121
996773
31
1742
319.4
34.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.814 ± 0.071
0.862 ± 0.017
5.755 ± 0.031
5.173 ± 0.038
3.703 ± 0.026
8.56 ± 0.042
1.968 ± 0.023
5.151 ± 0.033
3.467 ± 0.033
10.06 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.412 ± 0.021
2.714 ± 0.03
5.394 ± 0.035
3.642 ± 0.027
6.733 ± 0.04
5.549 ± 0.031
5.405 ± 0.032
7.119 ± 0.039
1.417 ± 0.018
2.101 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here