Candidatus Phycosocius bacilliformis 
Average proteome isoelectric point is 6.58 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 3121 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A2P2EDJ2|A0A2P2EDJ2_9PROT Uncharacterized protein OS=Candidatus Phycosocius bacilliformis OX=1445552 GN=PbB2_02819 PE=4 SV=1MM1 pKa = 7.17  SQEE4 pKa = 4.09  QSNQAYY10 pKa = 9.49  YY11 pKa = 10.91  AGRR14 pKa = 11.84  TDD16 pKa = 3.19  AFNGVGQQTIADD28 pKa = 3.76  QSSNLQTDD36 pKa = 3.57  YY37 pKa = 11.58  DD38 pKa = 3.55  QGYY41 pKa = 9.78  RR42 pKa = 11.84  DD43 pKa = 4.1  ANSEE47 pKa = 3.95  IKK49 pKa = 10.47  LDD51 pKa = 5.24  QDD53 pKa = 4.31  TEE55 pKa = 3.84  WDD57 pKa = 3.32  
 6.47 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.78 
IPC2_protein 3.681 
IPC_protein 3.592 
Toseland    3.389 
ProMoST     3.706 
Dawson      3.617 
Bjellqvist  3.872 
Wikipedia   3.617 
Rodwell     3.439 
Grimsley    3.312 
Solomon     3.579 
Lehninger   3.541 
Nozaki      3.77 
DTASelect   3.999 
Thurlkill   3.49 
EMBOSS      3.617 
Sillero     3.732 
Patrickios  1.863 
IPC_peptide 3.567 
IPC2_peptide  3.694 
IPC2.peptide.svr19  3.768 
 Protein with the highest isoelectric point: 
>tr|A0A2P2EDU5|A0A2P2EDU5_9PROT NAD(P) transhydrogenase subunit alpha part 1 OS=Candidatus Phycosocius bacilliformis OX=1445552 GN=pntAA PE=3 SV=1MM1 pKa = 7.5  GLACGCLTLSLALASPACAQSAKK24 pKa = 10.18  RR25 pKa = 11.84  GQEE28 pKa = 3.56  LTFNDD33 pKa = 3.76  MFEE36 pKa = 4.68  SPNAAAPQAVAFAAGPNNLRR56 pKa = 11.84  FVLDD60 pKa = 4.2  RR61 pKa = 11.84  SQPRR65 pKa = 11.84  VVLLQFDD72 pKa = 4.3  GDD74 pKa = 4.17  PEE76 pKa = 4.31  VWALTSSWGPRR87 pKa = 11.84  GDD89 pKa = 3.2  EE90 pKa = 4.45  FLRR93 pKa = 11.84  NDD95 pKa = 3.07  IGEE98 pKa = 4.33  VIVRR102 pKa = 11.84  ITTLGGVTVYY112 pKa = 11.1  GPLGTNGGLAASQGKK127 pKa = 9.65  AKK129 pKa = 10.41  SLPAPSRR136 pKa = 11.84  SEE138 pKa = 3.81  GTLQATLQKK147 pKa = 10.64  ALRR150 pKa = 11.84  WFDD153 pKa = 3.53  RR154 pKa = 11.84  FSSRR158 pKa = 11.84  SIRR161 pKa = 11.84  VEE163 pKa = 3.63  ASGGLPPALVHH174 pKa = 6.23  EE175 pKa = 4.62  ALEE178 pKa = 4.36  RR179 pKa = 11.84  AAQGMSRR186 pKa = 11.84  APRR189 pKa = 11.84  GYY191 pKa = 9.62  FGRR194 pKa = 11.84  PEE196 pKa = 4.07  RR197 pKa = 11.84  KK198 pKa = 8.74  VKK200 pKa = 10.68  LIRR203 pKa = 11.84  VQRR206 pKa = 11.84  SFDD209 pKa = 3.52  QPSVRR214 pKa = 11.84  WQSGVLTIGVVPGAGYY230 pKa = 10.7  AGRR233 pKa = 11.84  PSSAAVLLALTRR245 pKa = 11.84  SRR247 pKa = 4.21  
 26.24 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.354 
IPC2_protein 9.458 
IPC_protein 10.321 
Toseland    10.584 
ProMoST     10.57 
Dawson      10.672 
Bjellqvist  10.423 
Wikipedia   10.906 
Rodwell     10.76 
Grimsley    10.73 
Solomon     10.818 
Lehninger   10.789 
Nozaki      10.584 
DTASelect   10.409 
Thurlkill   10.584 
EMBOSS      10.994 
Sillero     10.613 
Patrickios  10.496 
IPC_peptide 10.833 
IPC2_peptide  9.589 
IPC2.peptide.svr19  8.747 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        3121 
0
3121 
996773
31
1742
319.4
34.54
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        12.814 ± 0.071
0.862 ± 0.017
5.755 ± 0.031
5.173 ± 0.038
3.703 ± 0.026
8.56 ± 0.042
1.968 ± 0.023
5.151 ± 0.033
3.467 ± 0.033
10.06 ± 0.046
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.412 ± 0.021
2.714 ± 0.03
5.394 ± 0.035
3.642 ± 0.027
6.733 ± 0.04
5.549 ± 0.031
5.405 ± 0.032
7.119 ± 0.039
1.417 ± 0.018
2.101 ± 0.021
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here