Amino acid dipepetide frequency for Anaerobiospirillum thomasii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.531AlaAla: 6.531 ± 0.123	1.303AlaCys: 1.303 ± 0.046	4.325AlaAsp: 4.325 ± 0.076	3.396AlaGlu: 3.396 ± 0.086	3.503AlaPhe: 3.503 ± 0.084	4.797AlaGly: 4.797 ± 0.098	1.558AlaHis: 1.558 ± 0.047	6.152AlaIle: 6.152 ± 0.092	4.898AlaLys: 4.898 ± 0.075	9.829AlaLeu: 9.829 ± 0.135	2.535AlaMet: 2.535 ± 0.06	3.259AlaAsn: 3.259 ± 0.078	2.41AlaPro: 2.41 ± 0.067	3.3AlaGln: 3.3 ± 0.073	3.574AlaArg: 3.574 ± 0.077	5.556AlaSer: 5.556 ± 0.08	4.083AlaThr: 4.083 ± 0.073	5.557AlaVal: 5.557 ± 0.088	0.466AlaTrp: 0.466 ± 0.028	2.674AlaTyr: 2.674 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.284CysAla: 1.284 ± 0.044	0.245CysCys: 0.245 ± 0.017	0.965CysAsp: 0.965 ± 0.035	0.837CysGlu: 0.837 ± 0.035	0.688CysPhe: 0.688 ± 0.028	1.099CysGly: 1.099 ± 0.045	0.434CysHis: 0.434 ± 0.027	1.087CysIle: 1.087 ± 0.036	0.925CysLys: 0.925 ± 0.03	1.355CysLeu: 1.355 ± 0.042	0.363CysMet: 0.363 ± 0.023	0.735CysAsn: 0.735 ± 0.031	0.606CysPro: 0.606 ± 0.031	0.496CysGln: 0.496 ± 0.027	0.684CysArg: 0.684 ± 0.031	1.209CysSer: 1.209 ± 0.041	0.796CysThr: 0.796 ± 0.027	0.939CysVal: 0.939 ± 0.032	0.074CysTrp: 0.074 ± 0.009	0.535CysTyr: 0.535 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	4.526AspAla: 4.526 ± 0.079	0.853AspCys: 0.853 ± 0.033	4.014AspAsp: 4.014 ± 0.091	4.622AspGlu: 4.622 ± 0.091	3.247AspPhe: 3.247 ± 0.068	3.882AspGly: 3.882 ± 0.07	0.895AspHis: 0.895 ± 0.035	5.122AspIle: 5.122 ± 0.093	5.009AspLys: 5.009 ± 0.087	5.923AspLeu: 5.923 ± 0.101	1.613AspMet: 1.613 ± 0.049	3.638AspAsn: 3.638 ± 0.081	1.925AspPro: 1.925 ± 0.06	1.108AspGln: 1.108 ± 0.044	2.996AspArg: 2.996 ± 0.064	3.962AspSer: 3.962 ± 0.076	3.282AspThr: 3.282 ± 0.07	3.95AspVal: 3.95 ± 0.062	0.36AspTrp: 0.36 ± 0.024	2.413AspTyr: 2.413 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	4.336GluAla: 4.336 ± 0.084	0.794GluCys: 0.794 ± 0.037	3.235GluAsp: 3.235 ± 0.072	3.022GluGlu: 3.022 ± 0.087	2.491GluPhe: 2.491 ± 0.061	3.219GluGly: 3.219 ± 0.068	1.718GluHis: 1.718 ± 0.05	4.591GluIle: 4.591 ± 0.072	3.975GluLys: 3.975 ± 0.084	6.285GluLeu: 6.285 ± 0.104	1.471GluMet: 1.471 ± 0.042	3.293GluAsn: 3.293 ± 0.063	1.633GluPro: 1.633 ± 0.049	2.741GluGln: 2.741 ± 0.055	2.686GluArg: 2.686 ± 0.062	4.05GluSer: 4.05 ± 0.081	2.219GluThr: 2.219 ± 0.05	3.518GluVal: 3.518 ± 0.068	0.301GluTrp: 0.301 ± 0.02	2.137GluTyr: 2.137 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.636PheAla: 3.636 ± 0.079	0.705PheCys: 0.705 ± 0.033	3.263PheAsp: 3.263 ± 0.075	2.697PheGlu: 2.697 ± 0.058	1.987PhePhe: 1.987 ± 0.052	2.854PheGly: 2.854 ± 0.06	0.591PheHis: 0.591 ± 0.026	3.921PheIle: 3.921 ± 0.078	3.224PheLys: 3.224 ± 0.066	3.769PheLeu: 3.769 ± 0.077	1.214PheMet: 1.214 ± 0.04	2.496PheAsn: 2.496 ± 0.055	1.007PhePro: 1.007 ± 0.037	0.674PheGln: 0.674 ± 0.031	1.401PheArg: 1.401 ± 0.038	3.038PheSer: 3.038 ± 0.06	2.55PheThr: 2.55 ± 0.052	2.992PheVal: 2.992 ± 0.073	0.276PheTrp: 0.276 ± 0.02	1.608PheTyr: 1.608 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.849GlyAla: 4.849 ± 0.105	1.061GlyCys: 1.061 ± 0.036	3.144GlyAsp: 3.144 ± 0.073	2.82GlyGlu: 2.82 ± 0.065	3.297GlyPhe: 3.297 ± 0.061	3.916GlyGly: 3.916 ± 0.091	1.376GlyHis: 1.376 ± 0.044	4.996GlyIle: 4.996 ± 0.087	3.691GlyLys: 3.691 ± 0.079	5.708GlyLeu: 5.708 ± 0.095	1.78GlyMet: 1.78 ± 0.051	2.612GlyAsn: 2.612 ± 0.059	1.547GlyPro: 1.547 ± 0.047	2.134GlyGln: 2.134 ± 0.05	3.048GlyArg: 3.048 ± 0.071	4.729GlySer: 4.729 ± 0.087	3.361GlyThr: 3.361 ± 0.072	4.428GlyVal: 4.428 ± 0.081	0.419GlyTrp: 0.419 ± 0.024	2.515GlyTyr: 2.515 ± 0.066	0.0GlyXaa: 0.0 ± 0.0
His	1.312HisAla: 1.312 ± 0.046	0.305HisCys: 0.305 ± 0.019	1.278HisAsp: 1.278 ± 0.054	1.178HisGlu: 1.178 ± 0.041	0.838HisPhe: 0.838 ± 0.038	1.337HisGly: 1.337 ± 0.051	0.429HisHis: 0.429 ± 0.029	1.594HisIle: 1.594 ± 0.047	1.441HisLys: 1.441 ± 0.044	1.834HisLeu: 1.834 ± 0.046	0.497HisMet: 0.497 ± 0.024	1.168HisAsn: 1.168 ± 0.042	0.744HisPro: 0.744 ± 0.03	0.604HisGln: 0.604 ± 0.028	0.778HisArg: 0.778 ± 0.033	1.246HisSer: 1.246 ± 0.036	1.017HisThr: 1.017 ± 0.035	1.123HisVal: 1.123 ± 0.037	0.113HisTrp: 0.113 ± 0.011	0.705HisTyr: 0.705 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.765IleAla: 6.765 ± 0.099	1.31IleCys: 1.31 ± 0.045	5.525IleAsp: 5.525 ± 0.095	4.921IleGlu: 4.921 ± 0.078	3.194IlePhe: 3.194 ± 0.073	4.478IleGly: 4.478 ± 0.089	1.138IleHis: 1.138 ± 0.04	5.38IleIle: 5.38 ± 0.106	5.79IleLys: 5.79 ± 0.101	6.308IleLeu: 6.308 ± 0.098	1.654IleMet: 1.654 ± 0.05	4.011IleAsn: 4.011 ± 0.086	2.594IlePro: 2.594 ± 0.056	1.98IleGln: 1.98 ± 0.053	3.139IleArg: 3.139 ± 0.075	5.53IleSer: 5.53 ± 0.089	3.901IleThr: 3.901 ± 0.071	4.925IleVal: 4.925 ± 0.086	0.414IleTrp: 0.414 ± 0.024	2.823IleTyr: 2.823 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	6.132LysAla: 6.132 ± 0.097	0.786LysCys: 0.786 ± 0.031	5.241LysAsp: 5.241 ± 0.09	5.18LysGlu: 5.18 ± 0.092	2.053LysPhe: 2.053 ± 0.057	4.198LysGly: 4.198 ± 0.081	1.171LysHis: 1.171 ± 0.038	4.823LysIle: 4.823 ± 0.088	5.178LysLys: 5.178 ± 0.086	5.772LysLeu: 5.772 ± 0.097	1.613LysMet: 1.613 ± 0.042	3.689LysAsn: 3.689 ± 0.076	1.864LysPro: 1.864 ± 0.053	2.081LysGln: 2.081 ± 0.054	2.907LysArg: 2.907 ± 0.066	4.758LysSer: 4.758 ± 0.088	3.791LysThr: 3.791 ± 0.067	4.129LysVal: 4.129 ± 0.076	0.422LysTrp: 0.422 ± 0.021	2.12LysTyr: 2.12 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	6.83LeuAla: 6.83 ± 0.105	2.018LeuCys: 2.018 ± 0.054	6.438LeuAsp: 6.438 ± 0.102	5.818LeuGlu: 5.818 ± 0.097	4.164LeuPhe: 4.164 ± 0.079	5.956LeuGly: 5.956 ± 0.112	1.937LeuHis: 1.937 ± 0.058	6.876LeuIle: 6.876 ± 0.101	7.504LeuLys: 7.504 ± 0.11	8.63LeuLeu: 8.63 ± 0.131	2.521LeuMet: 2.521 ± 0.062	5.428LeuAsn: 5.428 ± 0.096	3.622LeuPro: 3.622 ± 0.068	3.03LeuGln: 3.03 ± 0.059	4.017LeuArg: 4.017 ± 0.075	8.318LeuSer: 8.318 ± 0.121	5.072LeuThr: 5.072 ± 0.084	5.565LeuVal: 5.565 ± 0.089	0.635LeuTrp: 0.635 ± 0.032	3.569LeuTyr: 3.569 ± 0.073	0.0LeuXaa: 0.0 ± 0.0
Met	2.462MetAla: 2.462 ± 0.053	0.363MetCys: 0.363 ± 0.021	1.325MetAsp: 1.325 ± 0.036	1.199MetGlu: 1.199 ± 0.044	0.943MetPhe: 0.943 ± 0.029	1.544MetGly: 1.544 ± 0.049	0.688MetHis: 0.688 ± 0.029	1.658MetIle: 1.658 ± 0.042	1.195MetLys: 1.195 ± 0.034	2.941MetLeu: 2.941 ± 0.059	0.63MetMet: 0.63 ± 0.03	0.991MetAsn: 0.991 ± 0.039	1.274MetPro: 1.274 ± 0.045	1.429MetGln: 1.429 ± 0.042	1.301MetArg: 1.301 ± 0.048	2.142MetSer: 2.142 ± 0.053	1.542MetThr: 1.542 ± 0.045	1.58MetVal: 1.58 ± 0.044	0.163MetTrp: 0.163 ± 0.017	0.734MetTyr: 0.734 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.967AsnAla: 3.967 ± 0.073	0.693AsnCys: 0.693 ± 0.027	2.895AsnAsp: 2.895 ± 0.061	2.839AsnGlu: 2.839 ± 0.055	2.14AsnPhe: 2.14 ± 0.049	3.173AsnGly: 3.173 ± 0.07	0.792AsnHis: 0.792 ± 0.031	4.218AsnIle: 4.218 ± 0.089	3.402AsnLys: 3.402 ± 0.078	4.775AsnLeu: 4.775 ± 0.086	1.325AsnMet: 1.325 ± 0.045	2.728AsnAsn: 2.728 ± 0.073	1.796AsnPro: 1.796 ± 0.048	1.456AsnGln: 1.456 ± 0.045	2.024AsnArg: 2.024 ± 0.05	3.381AsnSer: 3.381 ± 0.084	2.894AsnThr: 2.894 ± 0.061	3.007AsnVal: 3.007 ± 0.062	0.302AsnTrp: 0.302 ± 0.02	1.889AsnTyr: 1.889 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.298ProAla: 2.298 ± 0.061	0.473ProCys: 0.473 ± 0.025	2.137ProAsp: 2.137 ± 0.055	2.157ProGlu: 2.157 ± 0.059	1.558ProPhe: 1.558 ± 0.04	1.791ProGly: 1.791 ± 0.054	0.654ProHis: 0.654 ± 0.03	1.839ProIle: 1.839 ± 0.052	1.925ProLys: 1.925 ± 0.053	3.296ProLeu: 3.296 ± 0.077	0.74ProMet: 0.74 ± 0.03	1.328ProAsn: 1.328 ± 0.041	0.909ProPro: 0.909 ± 0.039	1.273ProGln: 1.273 ± 0.055	1.171ProArg: 1.171 ± 0.04	2.156ProSer: 2.156 ± 0.054	1.447ProThr: 1.447 ± 0.042	2.776ProVal: 2.776 ± 0.06	0.336ProTrp: 0.336 ± 0.022	1.421ProTyr: 1.421 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.648GlnAla: 2.648 ± 0.073	0.469GlnCys: 0.469 ± 0.025	2.008GlnAsp: 2.008 ± 0.052	2.171GlnGlu: 2.171 ± 0.061	0.986GlnPhe: 0.986 ± 0.037	2.231GlnGly: 2.231 ± 0.048	0.517GlnHis: 0.517 ± 0.02	2.717GlnIle: 2.717 ± 0.055	2.745GlnLys: 2.745 ± 0.055	2.761GlnLeu: 2.761 ± 0.064	0.985GlnMet: 0.985 ± 0.039	1.806GlnAsn: 1.806 ± 0.062	0.762GlnPro: 0.762 ± 0.036	1.313GlnGln: 1.313 ± 0.044	1.87GlnArg: 1.87 ± 0.06	2.889GlnSer: 2.889 ± 0.066	1.798GlnThr: 1.798 ± 0.057	1.99GlnVal: 1.99 ± 0.051	0.217GlnTrp: 0.217 ± 0.017	1.175GlnTyr: 1.175 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	3.653ArgAla: 3.653 ± 0.081	0.467ArgCys: 0.467 ± 0.024	2.618ArgAsp: 2.618 ± 0.058	2.742ArgGlu: 2.742 ± 0.062	2.092ArgPhe: 2.092 ± 0.046	2.446ArgGly: 2.446 ± 0.062	1.109ArgHis: 1.109 ± 0.038	3.156ArgIle: 3.156 ± 0.066	2.306ArgLys: 2.306 ± 0.052	4.433ArgLeu: 4.433 ± 0.083	1.199ArgMet: 1.199 ± 0.042	2.016ArgAsn: 2.016 ± 0.046	1.467ArgPro: 1.467 ± 0.049	1.955ArgGln: 1.955 ± 0.055	2.247ArgArg: 2.247 ± 0.065	2.9ArgSer: 2.9 ± 0.063	1.884ArgThr: 1.884 ± 0.05	2.952ArgVal: 2.952 ± 0.068	0.307ArgTrp: 0.307 ± 0.022	1.906ArgTyr: 1.906 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	5.608SerAla: 5.608 ± 0.093	1.083SerCys: 1.083 ± 0.034	4.586SerAsp: 4.586 ± 0.077	3.559SerGlu: 3.559 ± 0.078	3.731SerPhe: 3.731 ± 0.082	4.611SerGly: 4.611 ± 0.079	1.393SerHis: 1.393 ± 0.038	5.559SerIle: 5.559 ± 0.088	4.393SerLys: 4.393 ± 0.072	7.675SerLeu: 7.675 ± 0.113	1.964SerMet: 1.964 ± 0.05	3.037SerAsn: 3.037 ± 0.069	1.893SerPro: 1.893 ± 0.052	2.681SerGln: 2.681 ± 0.069	3.192SerArg: 3.192 ± 0.063	5.324SerSer: 5.324 ± 0.102	3.489SerThr: 3.489 ± 0.071	4.822SerVal: 4.822 ± 0.078	0.562SerTrp: 0.562 ± 0.028	2.84SerTyr: 2.84 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	4.307ThrAla: 4.307 ± 0.088	0.644ThrCys: 0.644 ± 0.036	3.552ThrAsp: 3.552 ± 0.069	2.543ThrGlu: 2.543 ± 0.081	2.26ThrPhe: 2.26 ± 0.059	3.5ThrGly: 3.5 ± 0.069	1.072ThrHis: 1.072 ± 0.043	3.788ThrIle: 3.788 ± 0.063	2.623ThrLys: 2.623 ± 0.054	5.969ThrLeu: 5.969 ± 0.09	1.197ThrMet: 1.197 ± 0.036	2.095ThrAsn: 2.095 ± 0.061	1.919ThrPro: 1.919 ± 0.053	2.221ThrGln: 2.221 ± 0.058	2.037ThrArg: 2.037 ± 0.051	3.047ThrSer: 3.047 ± 0.066	2.936ThrThr: 2.936 ± 0.103	3.802ThrVal: 3.802 ± 0.079	0.349ThrTrp: 0.349 ± 0.021	1.869ThrTyr: 1.869 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	5.26ValAla: 5.26 ± 0.09	1.052ValCys: 1.052 ± 0.032	4.031ValAsp: 4.031 ± 0.076	3.554ValGlu: 3.554 ± 0.071	2.81ValPhe: 2.81 ± 0.066	3.843ValGly: 3.843 ± 0.086	1.211ValHis: 1.211 ± 0.037	5.124ValIle: 5.124 ± 0.091	4.557ValLys: 4.557 ± 0.074	6.299ValLeu: 6.299 ± 0.09	1.682ValMet: 1.682 ± 0.046	3.241ValAsn: 3.241 ± 0.075	2.351ValPro: 2.351 ± 0.058	2.151ValGln: 2.151 ± 0.07	2.73ValArg: 2.73 ± 0.062	4.847ValSer: 4.847 ± 0.09	3.453ValThr: 3.453 ± 0.072	4.173ValVal: 4.173 ± 0.097	0.365ValTrp: 0.365 ± 0.024	2.235ValTyr: 2.235 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.415TrpAla: 0.415 ± 0.02	0.104TrpCys: 0.104 ± 0.011	0.393TrpAsp: 0.393 ± 0.027	0.292TrpGlu: 0.292 ± 0.02	0.315TrpPhe: 0.315 ± 0.02	0.405TrpGly: 0.405 ± 0.024	0.192TrpHis: 0.192 ± 0.017	0.414TrpIle: 0.414 ± 0.025	0.313TrpLys: 0.313 ± 0.022	0.734TrpLeu: 0.734 ± 0.03	0.19TrpMet: 0.19 ± 0.014	0.235TrpAsn: 0.235 ± 0.019	0.213TrpPro: 0.213 ± 0.014	0.343TrpGln: 0.343 ± 0.023	0.335TrpArg: 0.335 ± 0.024	0.526TrpSer: 0.526 ± 0.028	0.237TrpThr: 0.237 ± 0.018	0.38TrpVal: 0.38 ± 0.026	0.075TrpTrp: 0.075 ± 0.011	0.271TrpTyr: 0.271 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.887TyrAla: 2.887 ± 0.065	0.628TyrCys: 0.628 ± 0.03	2.366TyrAsp: 2.366 ± 0.057	2.167TyrGlu: 2.167 ± 0.055	1.605TyrPhe: 1.605 ± 0.041	2.15TyrGly: 2.15 ± 0.049	0.666TyrHis: 0.666 ± 0.028	2.764TyrIle: 2.764 ± 0.059	2.814TyrLys: 2.814 ± 0.063	3.655TyrLeu: 3.655 ± 0.066	0.931TyrMet: 0.931 ± 0.032	1.891TyrAsn: 1.891 ± 0.054	1.15TyrPro: 1.15 ± 0.035	1.093TyrGln: 1.093 ± 0.041	1.719TyrArg: 1.719 ± 0.049	2.391TyrSer: 2.391 ± 0.061	1.978TyrThr: 1.978 ± 0.056	2.361TyrVal: 2.361 ± 0.054	0.225TyrTrp: 0.225 ± 0.017	1.447TyrTyr: 1.447 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2368 proteins (796788 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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