Candidatus Electrothrix communis
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1454 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S3QYK6|A0A3S3QYK6_9DELT Septal ring factor EnvC activator of murein hydrolases AmiA and AmiB (Fragment) OS=Candidatus Electrothrix communis OX=1859133 GN=VT98_13112 PE=4 SV=1
MM1 pKa = 7.19 VAGNAQAVEE10 pKa = 3.94 HH11 pKa = 6.93 RR12 pKa = 11.84 IGGGANYY19 pKa = 6.93 WTSVDD24 pKa = 6.02 DD25 pKa = 4.52 IDD27 pKa = 5.88 LDD29 pKa = 5.18 KK30 pKa = 11.02 IDD32 pKa = 5.49 DD33 pKa = 4.44 DD34 pKa = 4.52 GFSFLASYY42 pKa = 8.11 QFWPGLIGLEE52 pKa = 4.13 LDD54 pKa = 4.02 LEE56 pKa = 4.49 IVPDD60 pKa = 3.73 QYY62 pKa = 11.94 GEE64 pKa = 3.91 TAYY67 pKa = 10.52 APQAYY72 pKa = 9.98 ALIGKK77 pKa = 9.43 AIYY80 pKa = 9.97 AGAGIGINYY89 pKa = 9.31 RR90 pKa = 11.84 DD91 pKa = 4.01 GDD93 pKa = 4.01 LADD96 pKa = 4.18 EE97 pKa = 4.85 PFFSLRR103 pKa = 11.84 AGLNLEE109 pKa = 4.3 VLPGIYY115 pKa = 9.13 WDD117 pKa = 3.09 IYY119 pKa = 9.8 GNYY122 pKa = 9.22 RR123 pKa = 11.84 FNDD126 pKa = 3.63 TTDD129 pKa = 4.49 LEE131 pKa = 4.41 DD132 pKa = 3.9 HH133 pKa = 6.33 STDD136 pKa = 3.39 IDD138 pKa = 3.94 SDD140 pKa = 4.39 TIFLGSAVRR149 pKa = 11.84 IAII152 pKa = 4.09
Molecular weight: 16.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.719
IPC_protein 3.719
Toseland 3.49
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.706
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.126
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.846
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.78
Protein with the highest isoelectric point:
>tr|A0A3S3RB49|A0A3S3RB49_9DELT Transcription-repair-coupling factor OS=Candidatus Electrothrix communis OX=1859133 GN=mfd PE=3 SV=1
MM1 pKa = 7.38 IRR3 pKa = 11.84 GEE5 pKa = 4.34 VKK7 pKa = 9.79 QQADD11 pKa = 3.31 IGTEE15 pKa = 3.5 ISGCFQLKK23 pKa = 9.08 TGQLQHH29 pKa = 6.55 HH30 pKa = 6.71 NFRR33 pKa = 11.84 FSWIQQIFQNRR44 pKa = 11.84 GADD47 pKa = 3.07 IAADD51 pKa = 3.62 KK52 pKa = 11.19 NLLARR57 pKa = 11.84 AAQEE61 pKa = 4.01 MTDD64 pKa = 3.29 QAGYY68 pKa = 10.93 GGFAVGPGHH77 pKa = 7.36 RR78 pKa = 11.84 DD79 pKa = 3.43 DD80 pKa = 4.55 RR81 pKa = 11.84 HH82 pKa = 6.37 SDD84 pKa = 3.39 PASGEE89 pKa = 4.15 GKK91 pKa = 10.35 LSNNRR96 pKa = 11.84 HH97 pKa = 5.69 APSSGRR103 pKa = 11.84 SQHH106 pKa = 5.59 RR107 pKa = 11.84 QIHH110 pKa = 5.48 RR111 pKa = 11.84 HH112 pKa = 5.26 TGTGHH117 pKa = 4.47 NQIRR121 pKa = 11.84 IDD123 pKa = 3.64 KK124 pKa = 9.51 KK125 pKa = 8.37 GSRR128 pKa = 11.84 MAACLQLSAEE138 pKa = 4.69 IYY140 pKa = 10.3 AALHH144 pKa = 4.67 KK145 pKa = 10.47 HH146 pKa = 5.33 GLLFLRR152 pKa = 11.84 DD153 pKa = 4.09 RR154 pKa = 11.84 LPKK157 pKa = 10.21 LRR159 pKa = 11.84 NRR161 pKa = 11.84 DD162 pKa = 3.37 IQSLLVQIFHH172 pKa = 7.03 RR173 pKa = 11.84 RR174 pKa = 11.84 QPGFSQADD182 pKa = 3.49 HH183 pKa = 6.86 NDD185 pKa = 2.85 FHH187 pKa = 8.7 ARR189 pKa = 11.84 LQFNHH194 pKa = 6.21 NLSTEE199 pKa = 4.43 IICISGKK206 pKa = 10.44 LSAFALLKK214 pKa = 9.42 RR215 pKa = 11.84 TYY217 pKa = 9.62 ILRR220 pKa = 11.84 RR221 pKa = 11.84 SSVFVTGRR229 pKa = 11.84 LFLLYY234 pKa = 10.54 CLLLSTNFFIFSRR247 pKa = 11.84 RR248 pKa = 11.84 ILFYY252 pKa = 11.32 NLLLCCNFFHH262 pKa = 7.92 LSRR265 pKa = 11.84 FVLVYY270 pKa = 10.81 SLL272 pKa = 4.19
Molecular weight: 31.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.531
IPC_protein 10.218
Toseland 10.452
ProMoST 10.16
Dawson 10.584
Bjellqvist 10.306
Wikipedia 10.789
Rodwell 10.804
Grimsley 10.643
Solomon 10.672
Lehninger 10.643
Nozaki 10.482
DTASelect 10.292
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.482
IPC_peptide 10.672
IPC2_peptide 9.487
IPC2.peptide.svr19 8.539
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1454
0
1454
300695
39
1036
206.8
23.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.033 ± 0.075
1.384 ± 0.03
5.373 ± 0.057
6.91 ± 0.071
4.24 ± 0.052
7.334 ± 0.065
2.102 ± 0.033
6.276 ± 0.052
5.659 ± 0.075
10.399 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.69 ± 0.035
3.616 ± 0.045
4.276 ± 0.057
4.218 ± 0.045
5.546 ± 0.054
5.962 ± 0.062
5.198 ± 0.056
6.856 ± 0.051
1.062 ± 0.022
2.865 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here