Enterobacteria phage T4 (Bacteriophage T4)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 278 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P39492|Y10C_BPT4 Uncharacterized 6.6 kDa protein in Gp24-hoc intergenic region OS=Enterobacteria phage T4 OX=10665 GN=y10C PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.05YY3 pKa = 10.82AIKK6 pKa = 9.76PWWAARR12 pKa = 11.84WEE14 pKa = 4.45TVEE17 pKa = 4.62PEE19 pKa = 4.09PEE21 pKa = 3.97EE22 pKa = 3.69PVYY25 pKa = 10.68TDD27 pKa = 3.56EE28 pKa = 4.06EE29 pKa = 4.68TVYY32 pKa = 11.1NEE34 pKa = 3.68PTINDD39 pKa = 4.85LIDD42 pKa = 3.12MEE44 pKa = 4.59MGHH47 pKa = 7.03DD48 pKa = 3.76YY49 pKa = 11.32SRR51 pKa = 4.75

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P39499|Y13F_BPT4 Uncharacterized 10.1 kDa protein in cd-pseT intergenic region OS=Enterobacteria phage T4 OX=10665 GN=y13F PE=4 SV=1
MM1 pKa = 7.58LNNNVVYY8 pKa = 10.39LGYY11 pKa = 9.93PGLPPNKK18 pKa = 10.07LEE20 pKa = 4.08GLMLEE25 pKa = 4.68LRR27 pKa = 11.84TVGPSFGLEE36 pKa = 3.62FRR38 pKa = 11.84FQDD41 pKa = 3.39TPRR44 pKa = 11.84RR45 pKa = 11.84GKK47 pKa = 10.32NYY49 pKa = 8.62TQMHH53 pKa = 6.53ILKK56 pKa = 9.79QRR58 pKa = 11.84FKK60 pKa = 8.99TRR62 pKa = 11.84AFVMHH67 pKa = 6.2YY68 pKa = 10.0KK69 pKa = 9.78PRR71 pKa = 11.84KK72 pKa = 8.48EE73 pKa = 3.95KK74 pKa = 10.73FF75 pKa = 3.01

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

273

5

278

55174

26

1289

198.5

22.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.273 ± 0.169

1.116 ± 0.071

6.22 ± 0.105

7.119 ± 0.171

4.455 ± 0.112

5.95 ± 0.195

1.78 ± 0.083

7.516 ± 0.128

8.145 ± 0.249

7.42 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.095

5.709 ± 0.135

3.445 ± 0.092

3.308 ± 0.092

4.35 ± 0.099

6.673 ± 0.147

5.628 ± 0.214

6.34 ± 0.098

1.45 ± 0.047

4.372 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski