Amino acid dipepetide frequency for Enterobacteria phage T4 (Bacteriophage T4)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.731AlaAla: 4.731 ± 0.363
0.417AlaCys: 0.417 ± 0.077
3.244AlaAsp: 3.244 ± 0.292
5.057AlaGlu: 5.057 ± 0.379
2.429AlaPhe: 2.429 ± 0.188
3.861AlaGly: 3.861 ± 0.315
1.341AlaHis: 1.341 ± 0.177
4.604AlaIle: 4.604 ± 0.278
5.002AlaLys: 5.002 ± 0.338
5.365AlaLeu: 5.365 ± 0.301
1.269AlaMet: 1.269 ± 0.157
3.353AlaAsn: 3.353 ± 0.316
2.121AlaPro: 2.121 ± 0.189
2.755AlaGln: 2.755 ± 0.235
2.827AlaArg: 2.827 ± 0.236
4.06AlaSer: 4.06 ± 0.284
2.9AlaThr: 2.9 ± 0.341
4.187AlaVal: 4.187 ± 0.27
0.906AlaTrp: 0.906 ± 0.12
2.302AlaTyr: 2.302 ± 0.209
0.0AlaXaa: 0.0 ± 0.0
Cys
0.689CysAla: 0.689 ± 0.098
0.181CysCys: 0.181 ± 0.067
0.816CysAsp: 0.816 ± 0.106
0.834CysGlu: 0.834 ± 0.13
0.381CysPhe: 0.381 ± 0.087
0.834CysGly: 0.834 ± 0.125
0.326CysHis: 0.326 ± 0.077
0.761CysIle: 0.761 ± 0.111
0.761CysLys: 0.761 ± 0.124
0.725CysLeu: 0.725 ± 0.113
0.272CysMet: 0.272 ± 0.071
0.562CysAsn: 0.562 ± 0.105
0.616CysPro: 0.616 ± 0.107
0.326CysGln: 0.326 ± 0.073
0.544CysArg: 0.544 ± 0.099
0.906CysSer: 0.906 ± 0.126
0.435CysThr: 0.435 ± 0.09
0.544CysVal: 0.544 ± 0.097
0.127CysTrp: 0.127 ± 0.05
0.526CysTyr: 0.526 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
3.679AspAla: 3.679 ± 0.262
0.689AspCys: 0.689 ± 0.096
4.422AspAsp: 4.422 ± 0.331
4.495AspGlu: 4.495 ± 0.365
3.389AspPhe: 3.389 ± 0.24
4.296AspGly: 4.296 ± 0.267
0.906AspHis: 0.906 ± 0.111
4.894AspIle: 4.894 ± 0.331
4.767AspLys: 4.767 ± 0.309
4.767AspLeu: 4.767 ± 0.29
1.903AspMet: 1.903 ± 0.206
2.827AspAsn: 2.827 ± 0.206
2.048AspPro: 2.048 ± 0.225
1.16AspGln: 1.16 ± 0.132
2.084AspArg: 2.084 ± 0.214
3.897AspSer: 3.897 ± 0.272
3.099AspThr: 3.099 ± 0.206
4.296AspVal: 4.296 ± 0.294
1.16AspTrp: 1.16 ± 0.166
3.426AspTyr: 3.426 ± 0.245
0.0AspXaa: 0.0 ± 0.0
Glu
4.731GluAla: 4.731 ± 0.34
0.942GluCys: 0.942 ± 0.145
3.915GluAsp: 3.915 ± 0.338
5.021GluGlu: 5.021 ± 0.305
3.172GluPhe: 3.172 ± 0.244
3.752GluGly: 3.752 ± 0.286
1.359GluHis: 1.359 ± 0.156
5.999GluIle: 5.999 ± 0.334
5.419GluLys: 5.419 ± 0.396
6.761GluLeu: 6.761 ± 0.389
2.048GluMet: 2.048 ± 0.22
3.716GluAsn: 3.716 ± 0.295
1.613GluPro: 1.613 ± 0.165
2.465GluGln: 2.465 ± 0.239
2.592GluArg: 2.592 ± 0.251
4.096GluSer: 4.096 ± 0.26
4.259GluThr: 4.259 ± 0.289
4.586GluVal: 4.586 ± 0.314
1.196GluTrp: 1.196 ± 0.163
3.552GluTyr: 3.552 ± 0.276
0.0GluXaa: 0.0 ± 0.0
Phe
2.193PheAla: 2.193 ± 0.179
0.544PheCys: 0.544 ± 0.109
3.081PheAsp: 3.081 ± 0.254
3.498PheGlu: 3.498 ± 0.212
1.377PhePhe: 1.377 ± 0.143
2.9PheGly: 2.9 ± 0.23
0.725PheHis: 0.725 ± 0.134
3.462PheIle: 3.462 ± 0.246
4.096PheLys: 4.096 ± 0.324
2.392PheLeu: 2.392 ± 0.217
1.377PheMet: 1.377 ± 0.187
3.009PheAsn: 3.009 ± 0.197
1.106PhePro: 1.106 ± 0.13
1.45PheGln: 1.45 ± 0.176
2.066PheArg: 2.066 ± 0.195
3.353PheSer: 3.353 ± 0.24
2.556PheThr: 2.556 ± 0.233
2.664PheVal: 2.664 ± 0.259
0.598PheTrp: 0.598 ± 0.097
2.102PheTyr: 2.102 ± 0.197
0.0PheXaa: 0.0 ± 0.0
Gly
2.755GlyAla: 2.755 ± 0.235
0.58GlyCys: 0.58 ± 0.104
3.716GlyAsp: 3.716 ± 0.296
3.48GlyGlu: 3.48 ± 0.268
2.846GlyPhe: 2.846 ± 0.219
3.516GlyGly: 3.516 ± 0.398
0.634GlyHis: 0.634 ± 0.12
4.223GlyIle: 4.223 ± 0.294
4.857GlyLys: 4.857 ± 0.337
4.749GlyLeu: 4.749 ± 0.318
1.994GlyMet: 1.994 ± 0.215
3.19GlyAsn: 3.19 ± 0.308
1.794GlyPro: 1.794 ± 0.211
2.247GlyGln: 2.247 ± 0.236
2.501GlyArg: 2.501 ± 0.175
4.096GlySer: 4.096 ± 0.323
3.987GlyThr: 3.987 ± 0.445
4.296GlyVal: 4.296 ± 0.296
1.106GlyTrp: 1.106 ± 0.149
2.936GlyTyr: 2.936 ± 0.214
0.0GlyXaa: 0.0 ± 0.0
His
0.779HisAla: 0.779 ± 0.107
0.217HisCys: 0.217 ± 0.059
0.942HisAsp: 0.942 ± 0.116
1.106HisGlu: 1.106 ± 0.162
1.106HisPhe: 1.106 ± 0.138
0.906HisGly: 0.906 ± 0.153
0.544HisHis: 0.544 ± 0.101
1.432HisIle: 1.432 ± 0.164
1.577HisLys: 1.577 ± 0.224
1.432HisLeu: 1.432 ± 0.152
0.362HisMet: 0.362 ± 0.083
0.562HisAsn: 0.562 ± 0.111
1.015HisPro: 1.015 ± 0.131
0.562HisGln: 0.562 ± 0.105
0.924HisArg: 0.924 ± 0.136
1.559HisSer: 1.559 ± 0.215
0.834HisThr: 0.834 ± 0.137
0.924HisVal: 0.924 ± 0.13
0.29HisTrp: 0.29 ± 0.072
0.725HisTyr: 0.725 ± 0.111
0.0HisXaa: 0.0 ± 0.0
Ile
4.948IleAla: 4.948 ± 0.352
0.761IleCys: 0.761 ± 0.12
5.492IleAsp: 5.492 ± 0.305
5.456IleGlu: 5.456 ± 0.376
2.664IlePhe: 2.664 ± 0.193
3.752IleGly: 3.752 ± 0.275
1.396IleHis: 1.396 ± 0.144
5.836IleIle: 5.836 ± 0.375
6.797IleLys: 6.797 ± 0.42
4.749IleLeu: 4.749 ± 0.305
1.667IleMet: 1.667 ± 0.169
5.039IleAsn: 5.039 ± 0.307
3.009IlePro: 3.009 ± 0.217
2.701IleGln: 2.701 ± 0.235
3.389IleArg: 3.389 ± 0.274
4.912IleSer: 4.912 ± 0.293
4.513IleThr: 4.513 ± 0.278
4.35IleVal: 4.35 ± 0.258
0.652IleTrp: 0.652 ± 0.119
3.081IleTyr: 3.081 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
5.764LysAla: 5.764 ± 0.393
1.069LysCys: 1.069 ± 0.137
5.474LysAsp: 5.474 ± 0.331
6.054LysGlu: 6.054 ± 0.468
4.114LysPhe: 4.114 ± 0.316
4.187LysGly: 4.187 ± 0.227
1.976LysHis: 1.976 ± 0.232
6.235LysIle: 6.235 ± 0.38
5.655LysLys: 5.655 ± 0.38
6.434LysLeu: 6.434 ± 0.33
2.429LysMet: 2.429 ± 0.185
4.368LysAsn: 4.368 ± 0.341
2.556LysPro: 2.556 ± 0.216
2.628LysGln: 2.628 ± 0.226
3.734LysArg: 3.734 ± 0.289
5.274LysSer: 5.274 ± 0.382
4.368LysThr: 4.368 ± 0.278
4.586LysVal: 4.586 ± 0.308
1.106LysTrp: 1.106 ± 0.14
3.444LysTyr: 3.444 ± 0.29
0.0LysXaa: 0.0 ± 0.0
Leu
5.166LeuAla: 5.166 ± 0.318
0.87LeuCys: 0.87 ± 0.123
4.731LeuAsp: 4.731 ± 0.364
5.147LeuGlu: 5.147 ± 0.412
3.226LeuPhe: 3.226 ± 0.259
4.114LeuGly: 4.114 ± 0.281
1.196LeuHis: 1.196 ± 0.155
5.437LeuIle: 5.437 ± 0.313
6.253LeuLys: 6.253 ± 0.346
4.821LeuLeu: 4.821 ± 0.363
2.338LeuMet: 2.338 ± 0.216
4.894LeuAsn: 4.894 ± 0.244
2.918LeuPro: 2.918 ± 0.27
2.411LeuGln: 2.411 ± 0.201
3.371LeuArg: 3.371 ± 0.237
4.984LeuSer: 4.984 ± 0.278
4.024LeuThr: 4.024 ± 0.33
4.531LeuVal: 4.531 ± 0.279
0.834LeuTrp: 0.834 ± 0.128
2.936LeuTyr: 2.936 ± 0.207
0.0LeuXaa: 0.0 ± 0.0
Met
2.048MetAla: 2.048 ± 0.197
0.344MetCys: 0.344 ± 0.07
1.359MetAsp: 1.359 ± 0.159
1.577MetGlu: 1.577 ± 0.16
1.196MetPhe: 1.196 ± 0.135
1.396MetGly: 1.396 ± 0.145
0.344MetHis: 0.344 ± 0.081
1.939MetIle: 1.939 ± 0.194
3.244MetLys: 3.244 ± 0.263
2.048MetLeu: 2.048 ± 0.214
1.033MetMet: 1.033 ± 0.143
1.758MetAsn: 1.758 ± 0.18
0.689MetPro: 0.689 ± 0.108
0.906MetGln: 0.906 ± 0.116
1.251MetArg: 1.251 ± 0.158
2.084MetSer: 2.084 ± 0.248
1.722MetThr: 1.722 ± 0.145
1.178MetVal: 1.178 ± 0.146
0.199MetTrp: 0.199 ± 0.063
0.942MetTyr: 0.942 ± 0.136
0.018MetXaa: 0.018 ± 0.016
Asn
3.607AsnAla: 3.607 ± 0.292
0.544AsnCys: 0.544 ± 0.095
3.389AsnAsp: 3.389 ± 0.277
3.969AsnGlu: 3.969 ± 0.272
2.646AsnPhe: 2.646 ± 0.208
4.006AsnGly: 4.006 ± 0.358
0.761AsnHis: 0.761 ± 0.104
4.441AsnIle: 4.441 ± 0.258
4.676AsnLys: 4.676 ± 0.291
4.277AsnLeu: 4.277 ± 0.235
1.522AsnMet: 1.522 ± 0.196
3.498AsnAsn: 3.498 ± 0.264
2.429AsnPro: 2.429 ± 0.21
1.613AsnGln: 1.613 ± 0.188
2.574AsnArg: 2.574 ± 0.188
3.879AsnSer: 3.879 ± 0.257
2.791AsnThr: 2.791 ± 0.268
3.208AsnVal: 3.208 ± 0.267
0.743AsnTrp: 0.743 ± 0.129
2.519AsnTyr: 2.519 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
1.849ProAla: 1.849 ± 0.198
0.453ProCys: 0.453 ± 0.087
2.719ProAsp: 2.719 ± 0.231
3.172ProGlu: 3.172 ± 0.271
1.559ProPhe: 1.559 ± 0.162
2.392ProGly: 2.392 ± 0.244
0.562ProHis: 0.562 ± 0.096
2.012ProIle: 2.012 ± 0.225
2.392ProLys: 2.392 ± 0.234
2.157ProLeu: 2.157 ± 0.185
0.761ProMet: 0.761 ± 0.115
2.012ProAsn: 2.012 ± 0.178
0.997ProPro: 0.997 ± 0.155
1.015ProGln: 1.015 ± 0.122
1.269ProArg: 1.269 ± 0.185
2.211ProSer: 2.211 ± 0.215
2.139ProThr: 2.139 ± 0.228
2.574ProVal: 2.574 ± 0.213
0.689ProTrp: 0.689 ± 0.118
1.522ProTyr: 1.522 ± 0.165
0.0ProXaa: 0.0 ± 0.0
Gln
2.229GlnAla: 2.229 ± 0.211
0.29GlnCys: 0.29 ± 0.076
1.595GlnAsp: 1.595 ± 0.186
2.392GlnGlu: 2.392 ± 0.245
1.667GlnPhe: 1.667 ± 0.178
1.794GlnGly: 1.794 ± 0.202
0.453GlnHis: 0.453 ± 0.1
2.574GlnIle: 2.574 ± 0.191
2.338GlnLys: 2.338 ± 0.236
2.682GlnLeu: 2.682 ± 0.239
0.924GlnMet: 0.924 ± 0.121
1.649GlnAsn: 1.649 ± 0.181
1.16GlnPro: 1.16 ± 0.136
0.979GlnGln: 0.979 ± 0.149
1.903GlnArg: 1.903 ± 0.17
2.084GlnSer: 2.084 ± 0.206
2.012GlnThr: 2.012 ± 0.222
2.084GlnVal: 2.084 ± 0.2
0.634GlnTrp: 0.634 ± 0.107
1.631GlnTyr: 1.631 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
2.664ArgAla: 2.664 ± 0.25
0.526ArgCys: 0.526 ± 0.112
2.682ArgAsp: 2.682 ± 0.209
3.571ArgGlu: 3.571 ± 0.277
1.939ArgPhe: 1.939 ± 0.178
2.954ArgGly: 2.954 ± 0.203
0.689ArgHis: 0.689 ± 0.118
3.136ArgIle: 3.136 ± 0.219
3.407ArgLys: 3.407 ± 0.278
3.625ArgLeu: 3.625 ± 0.279
0.961ArgMet: 0.961 ± 0.132
2.102ArgAsn: 2.102 ± 0.18
1.251ArgPro: 1.251 ± 0.167
1.667ArgGln: 1.667 ± 0.177
1.976ArgArg: 1.976 ± 0.189
2.646ArgSer: 2.646 ± 0.232
2.556ArgThr: 2.556 ± 0.235
2.737ArgVal: 2.737 ± 0.186
0.761ArgTrp: 0.761 ± 0.13
1.649ArgTyr: 1.649 ± 0.194
0.0ArgXaa: 0.0 ± 0.0
Ser
3.697SerAla: 3.697 ± 0.27
0.707SerCys: 0.707 ± 0.139
3.734SerAsp: 3.734 ± 0.257
4.169SerGlu: 4.169 ± 0.281
2.9SerPhe: 2.9 ± 0.231
4.567SerGly: 4.567 ± 0.26
1.287SerHis: 1.287 ± 0.175
5.147SerIle: 5.147 ± 0.331
5.854SerLys: 5.854 ± 0.335
4.93SerLeu: 4.93 ± 0.29
1.577SerMet: 1.577 ± 0.184
3.534SerAsn: 3.534 ± 0.255
2.537SerPro: 2.537 ± 0.211
1.921SerGln: 1.921 ± 0.15
2.846SerArg: 2.846 ± 0.224
5.456SerSer: 5.456 ± 0.377
4.114SerThr: 4.114 ± 0.332
3.915SerVal: 3.915 ± 0.254
1.033SerTrp: 1.033 ± 0.13
2.809SerTyr: 2.809 ± 0.22
0.0SerXaa: 0.0 ± 0.0
Thr
3.915ThrAla: 3.915 ± 0.368
0.507ThrCys: 0.507 ± 0.105
3.371ThrAsp: 3.371 ± 0.243
3.571ThrGlu: 3.571 ± 0.276
2.501ThrPhe: 2.501 ± 0.211
3.933ThrGly: 3.933 ± 0.375
1.124ThrHis: 1.124 ± 0.145
3.969ThrIle: 3.969 ± 0.328
3.915ThrLys: 3.915 ± 0.242
3.824ThrLeu: 3.824 ± 0.283
1.124ThrMet: 1.124 ± 0.152
3.117ThrAsn: 3.117 ± 0.265
2.61ThrPro: 2.61 ± 0.267
1.976ThrGln: 1.976 ± 0.234
2.556ThrArg: 2.556 ± 0.25
3.299ThrSer: 3.299 ± 0.321
2.954ThrThr: 2.954 ± 0.269
4.604ThrVal: 4.604 ± 0.403
0.797ThrTrp: 0.797 ± 0.136
2.61ThrTyr: 2.61 ± 0.205
0.0ThrXaa: 0.0 ± 0.0
Val
3.281ValAla: 3.281 ± 0.251
0.906ValCys: 0.906 ± 0.138
3.716ValAsp: 3.716 ± 0.25
5.111ValGlu: 5.111 ± 0.372
2.664ValPhe: 2.664 ± 0.232
3.571ValGly: 3.571 ± 0.299
1.069ValHis: 1.069 ± 0.125
4.567ValIle: 4.567 ± 0.244
5.619ValLys: 5.619 ± 0.317
4.495ValLeu: 4.495 ± 0.232
2.066ValMet: 2.066 ± 0.233
4.151ValAsn: 4.151 ± 0.286
1.903ValPro: 1.903 ± 0.188
2.048ValGln: 2.048 ± 0.246
2.664ValArg: 2.664 ± 0.243
4.205ValSer: 4.205 ± 0.248
3.842ValThr: 3.842 ± 0.311
4.259ValVal: 4.259 ± 0.294
0.779ValTrp: 0.779 ± 0.104
2.465ValTyr: 2.465 ± 0.177
0.0ValXaa: 0.0 ± 0.0
Trp
0.743TrpAla: 0.743 ± 0.118
0.127TrpCys: 0.127 ± 0.043
0.834TrpAsp: 0.834 ± 0.13
0.906TrpGlu: 0.906 ± 0.124
0.906TrpPhe: 0.906 ± 0.129
0.417TrpGly: 0.417 ± 0.096
0.217TrpHis: 0.217 ± 0.067
1.142TrpIle: 1.142 ± 0.145
1.559TrpLys: 1.559 ± 0.193
0.997TrpLeu: 0.997 ± 0.124
0.471TrpMet: 0.471 ± 0.087
1.069TrpAsn: 1.069 ± 0.154
0.417TrpPro: 0.417 ± 0.087
0.58TrpGln: 0.58 ± 0.093
0.471TrpArg: 0.471 ± 0.081
1.069TrpSer: 1.069 ± 0.113
0.689TrpThr: 0.689 ± 0.122
0.852TrpVal: 0.852 ± 0.112
0.272TrpTrp: 0.272 ± 0.07
0.761TrpTyr: 0.761 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.262TyrAla: 3.262 ± 0.223
0.471TyrCys: 0.471 ± 0.085
2.972TyrAsp: 2.972 ± 0.242
2.61TyrGlu: 2.61 ± 0.243
1.867TyrPhe: 1.867 ± 0.199
2.356TyrGly: 2.356 ± 0.217
0.888TyrHis: 0.888 ± 0.119
3.353TyrIle: 3.353 ± 0.239
3.262TyrLys: 3.262 ± 0.296
2.972TyrLeu: 2.972 ± 0.229
1.16TyrMet: 1.16 ± 0.132
2.682TyrAsn: 2.682 ± 0.249
1.667TyrPro: 1.667 ± 0.162
1.667TyrGln: 1.667 ± 0.167
1.957TyrArg: 1.957 ± 0.224
2.664TyrSer: 2.664 ± 0.228
2.483TyrThr: 2.483 ± 0.19
3.027TyrVal: 3.027 ± 0.244
0.616TyrTrp: 0.616 ± 0.105
1.776TyrTyr: 1.776 ± 0.211
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.018XaaCys: 0.018 ± 0.016
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 278 proteins (55174 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski