Sporothrix schenckii (strain ATCC 58251 / de Perez 2211183) (Rose-picker s disease fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Ophiostomatales; Ophiostomataceae; Sporothrix; Sporothrix schenckii

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U7PNR3|U7PNR3_SPOS1 Uncharacterized protein OS=Sporothrix schenckii (strain ATCC 58251 / de Perez 2211183) OX=1391915 GN=HMPREF1624_06558 PE=4 SV=1
MM1 pKa = 8.1SYY3 pKa = 11.59GNDD6 pKa = 3.71NNDD9 pKa = 2.76SGYY12 pKa = 10.86GGNSGGFGGNNNDD25 pKa = 3.78DD26 pKa = 3.8SSFGGSGGRR35 pKa = 11.84GGRR38 pKa = 11.84NDD40 pKa = 3.93DD41 pKa = 3.71NSFGSSGNSGNSGSDD56 pKa = 3.01SYY58 pKa = 12.18GSGNSGSGGFGGSSGDD74 pKa = 3.66DD75 pKa = 3.45SYY77 pKa = 12.21GSSGNSGNRR86 pKa = 11.84SGRR89 pKa = 11.84GDD91 pKa = 3.78DD92 pKa = 4.3DD93 pKa = 5.56SYY95 pKa = 11.92GSSGNSGNRR104 pKa = 11.84GGRR107 pKa = 11.84GGDD110 pKa = 3.47DD111 pKa = 3.62SYY113 pKa = 12.06GSSGNSGNSGSGGFGGSSGDD133 pKa = 3.66DD134 pKa = 3.45SYY136 pKa = 12.21GSSGNSGSRR145 pKa = 11.84GGRR148 pKa = 11.84GGDD151 pKa = 3.49DD152 pKa = 3.62SYY154 pKa = 12.06GSSGNSGNSGSDD166 pKa = 3.01SYY168 pKa = 12.18GSGNSGSGGFGGSSGDD184 pKa = 3.66DD185 pKa = 3.45SYY187 pKa = 12.21GSSGNSGNRR196 pKa = 11.84GGRR199 pKa = 11.84GGDD202 pKa = 3.47DD203 pKa = 3.62SYY205 pKa = 11.99GSSGNTGSGGYY216 pKa = 9.84GGNNNDD222 pKa = 4.38NNDD225 pKa = 3.26SNYY228 pKa = 11.1

Molecular weight: 21.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U7PP33|U7PP33_SPOS1 Uncharacterized protein OS=Sporothrix schenckii (strain ATCC 58251 / de Perez 2211183) OX=1391915 GN=HMPREF1624_07174 PE=4 SV=1
MM1 pKa = 7.3HH2 pKa = 7.26WHH4 pKa = 7.11HH5 pKa = 6.2STQTRR10 pKa = 11.84STGGILSPKK19 pKa = 8.99TRR21 pKa = 11.84TTRR24 pKa = 11.84TTHH27 pKa = 5.78RR28 pKa = 11.84HH29 pKa = 5.55HH30 pKa = 6.64GHH32 pKa = 6.82HH33 pKa = 6.46GHH35 pKa = 6.89HH36 pKa = 6.31SHH38 pKa = 6.92NGHH41 pKa = 5.01THH43 pKa = 5.01RR44 pKa = 11.84HH45 pKa = 4.76GLGLSTAGATTTTARR60 pKa = 11.84RR61 pKa = 11.84SPGTGLFHH69 pKa = 7.36RR70 pKa = 11.84RR71 pKa = 11.84VHH73 pKa = 5.25TPRR76 pKa = 11.84TKK78 pKa = 10.42VRR80 pKa = 11.84TARR83 pKa = 11.84TTTRR87 pKa = 11.84RR88 pKa = 11.84TGGGGGLFGTRR99 pKa = 11.84HH100 pKa = 5.2HH101 pKa = 6.0HH102 pKa = 5.29TKK104 pKa = 10.2RR105 pKa = 11.84SHH107 pKa = 5.42VLPVTTTTRR116 pKa = 11.84RR117 pKa = 11.84KK118 pKa = 7.93PTVGDD123 pKa = 4.01RR124 pKa = 11.84ISGAFHH130 pKa = 7.32KK131 pKa = 10.41IKK133 pKa = 10.66GSITGQPRR141 pKa = 11.84EE142 pKa = 3.95KK143 pKa = 10.71AIGDD147 pKa = 3.39RR148 pKa = 11.84RR149 pKa = 11.84MHH151 pKa = 5.28GTTRR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84WW159 pKa = 3.02

Molecular weight: 17.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8673

0

8673

4783716

29

6939

551.6

59.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.983 ± 0.034

1.013 ± 0.009

6.081 ± 0.021

5.302 ± 0.026

3.322 ± 0.014

7.64 ± 0.029

2.399 ± 0.011

3.892 ± 0.015

3.976 ± 0.02

8.398 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.009

3.476 ± 0.015

6.362 ± 0.027

4.032 ± 0.025

6.29 ± 0.023

8.278 ± 0.036

6.277 ± 0.018

6.432 ± 0.019

1.276 ± 0.008

2.521 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski