Streptomyces abyssalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5131 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E7JUN6|A0A1E7JUN6_9ACTN DUF126 domain-containing protein OS=Streptomyces abyssalis OX=933944 GN=AN215_02480 PE=4 SV=1
MM1 pKa = 7.16STGDD5 pKa = 3.67DD6 pKa = 3.85ALEE9 pKa = 3.85VWIDD13 pKa = 3.4QDD15 pKa = 3.99LCTGDD20 pKa = 5.01GICAQYY26 pKa = 10.9APEE29 pKa = 4.23VFEE32 pKa = 5.9LDD34 pKa = 3.05IDD36 pKa = 3.63GLAYY40 pKa = 10.39VKK42 pKa = 10.61SAEE45 pKa = 5.11DD46 pKa = 3.96EE47 pKa = 4.19LLQDD51 pKa = 4.34KK52 pKa = 10.59GATTPVPLPLLTDD65 pKa = 3.6VVDD68 pKa = 4.12SAKK71 pKa = 10.23EE72 pKa = 3.92CPGDD76 pKa = 4.18CIHH79 pKa = 6.28VRR81 pKa = 11.84RR82 pKa = 11.84VSDD85 pKa = 3.47SVEE88 pKa = 4.23VYY90 pKa = 10.85GPDD93 pKa = 3.98ADD95 pKa = 3.76EE96 pKa = 5.16HH97 pKa = 5.9GQQ99 pKa = 3.36

Molecular weight: 10.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E7JQ50|A0A1E7JQ50_9ACTN MarR family transcriptional regulator OS=Streptomyces abyssalis OX=933944 GN=AN215_13205 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.8GRR40 pKa = 11.84SRR42 pKa = 11.84LSAA45 pKa = 3.74

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5131

0

5131

1675583

37

4135

326.6

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.17 ± 0.049

0.813 ± 0.011

5.844 ± 0.025

6.289 ± 0.035

2.781 ± 0.021

10.057 ± 0.036

2.281 ± 0.016

3.007 ± 0.023

2.322 ± 0.031

10.057 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.879 ± 0.014

1.736 ± 0.017

5.951 ± 0.029

2.808 ± 0.02

8.06 ± 0.037

5.398 ± 0.025

5.75 ± 0.021

8.321 ± 0.03

1.448 ± 0.014

2.029 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski