Arthrobacter phage Beans
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A222ZJ07|A0A222ZJ07_9CAUD Uncharacterized protein OS=Arthrobacter phage Beans OX=2015815 GN=16 PE=4 SV=1
MM1 pKa = 7.89 ADD3 pKa = 3.48 GVLSFPFRR11 pKa = 11.84 LDD13 pKa = 3.07 TTGAIATVADD23 pKa = 4.2 GSDD26 pKa = 3.26 AYY28 pKa = 11.2 VDD30 pKa = 3.2 EE31 pKa = 5.93 HH32 pKa = 6.36 IAKK35 pKa = 10.28 LVLTNIGEE43 pKa = 4.61 CPMSPEE49 pKa = 4.09 YY50 pKa = 10.59 GVPDD54 pKa = 3.61 PTFAALHH61 pKa = 6.3 IGDD64 pKa = 4.04 VQAGLSTFGPSGVRR78 pKa = 11.84 VTAVEE83 pKa = 4.09 MTPFSDD89 pKa = 3.77 TQSVASIAWAYY100 pKa = 9.99 EE101 pKa = 3.61 NALGVTNNGG110 pKa = 3.22
Molecular weight: 11.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.948
IPC_protein 3.859
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.579
Solomon 3.846
Lehninger 3.795
Nozaki 3.999
DTASelect 4.202
Thurlkill 3.732
EMBOSS 3.821
Sillero 3.986
Patrickios 1.914
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.896
Protein with the highest isoelectric point:
>tr|A0A222ZJY6|A0A222ZJY6_9CAUD Uncharacterized protein OS=Arthrobacter phage Beans OX=2015815 GN=51 PE=4 SV=1
MM1 pKa = 7.21 TTPQNNPQAKK11 pKa = 8.68 QRR13 pKa = 11.84 KK14 pKa = 4.49 TTQRR18 pKa = 11.84 RR19 pKa = 11.84 AAAGRR24 pKa = 11.84 SAQKK28 pKa = 9.63 PRR30 pKa = 11.84 KK31 pKa = 8.57 RR32 pKa = 11.84 TALPKK37 pKa = 10.16 AAPKK41 pKa = 10.07 PLEE44 pKa = 4.37 PLTALGPLWPDD55 pKa = 3.73 PLPLEE60 pKa = 4.01 YY61 pKa = 10.05 HH62 pKa = 6.54 AEE64 pKa = 3.83 IEE66 pKa = 3.96 IDD68 pKa = 3.4 VRR70 pKa = 11.84 LWDD73 pKa = 3.57 WQKK76 pKa = 9.44 TRR78 pKa = 11.84 ISANDD83 pKa = 3.47 RR84 pKa = 11.84 LDD86 pKa = 3.49 PRR88 pKa = 11.84 EE89 pKa = 4.06 KK90 pKa = 10.64 NRR92 pKa = 11.84 RR93 pKa = 11.84 TQIWRR98 pKa = 11.84 SAAEE102 pKa = 4.04 AAVLAAGIDD111 pKa = 3.72 PLSWARR117 pKa = 11.84 VVYY120 pKa = 8.97 WVRR123 pKa = 11.84 WPDD126 pKa = 3.0 NRR128 pKa = 11.84 KK129 pKa = 9.69 RR130 pKa = 11.84 EE131 pKa = 4.11 TSNLQPTAKK140 pKa = 10.31 AIVDD144 pKa = 3.72 GMVDD148 pKa = 3.93 ARR150 pKa = 11.84 VLPDD154 pKa = 4.64 DD155 pKa = 4.36 RR156 pKa = 11.84 DD157 pKa = 3.71 EE158 pKa = 4.54 MVDD161 pKa = 3.56 GPDD164 pKa = 3.63 ARR166 pKa = 11.84 RR167 pKa = 11.84 IYY169 pKa = 10.48 PNGPHH174 pKa = 6.57 RR175 pKa = 11.84 VIIQLWRR182 pKa = 11.84 RR183 pKa = 11.84 AA184 pKa = 3.44
Molecular weight: 21.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.56
IPC_protein 10.365
Toseland 10.789
ProMoST 10.496
Dawson 10.847
Bjellqvist 10.555
Wikipedia 11.067
Rodwell 11.008
Grimsley 10.877
Solomon 11.008
Lehninger 10.965
Nozaki 10.745
DTASelect 10.555
Thurlkill 10.76
EMBOSS 11.184
Sillero 10.774
Patrickios 10.745
IPC_peptide 11.008
IPC2_peptide 9.253
IPC2.peptide.svr19 8.863
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
15703
42
875
215.1
23.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.367 ± 0.509
0.675 ± 0.092
6.196 ± 0.287
6.496 ± 0.397
2.764 ± 0.164
7.827 ± 0.386
1.618 ± 0.132
4.744 ± 0.229
4.286 ± 0.196
8.075 ± 0.313
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.331 ± 0.158
3.324 ± 0.186
5.139 ± 0.32
3.611 ± 0.191
6.394 ± 0.334
5.566 ± 0.269
6.827 ± 0.349
7.788 ± 0.321
1.528 ± 0.153
2.445 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here